Date published: 2025-9-7
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MCM2 Antibody (E-8) - Western Blotting - Image 356630
MCM2 抗体 (E-8): sc-373702. Western 印迹分析 Jurkat 全细胞裂解液中 MCM2 的表达.
MCM2 抗体 (E-8): sc-373702. Western 印迹分析 Jurkat 全细胞裂解液中 MCM2 的表达.

MCM2 抗体 (E-8): sc-373702

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说明书
  • MCM2 抗体 E-8 是小鼠单克隆 IgG2b κ，MCM2抗体, 在22篇文献中引用，规格为200 µg/ml
  • 特异性抗原位于human物种的MCM2的N-terminus的氨基酸9-34之间
  • MCM2 抗体 (E-8) 推荐用于 WB, IP, IF 和 ELISA，检测mouse, rat 和human 来源的 MCM2
  • 抗MCM2抗体（E-8）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取MCM2 (E-8): sc-373702的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • 2b BP-HRP">m-IgG2b BP-HRP和m-IgGκ BP-HRP是MCM2 Antibody (E-8) 适用于 WB 应用。 的首选辅助检测试剂。这些试剂现与MCM2 Antibody (E-8) 打包提供（请参阅下面的订购信息）。

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描述
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说明书与实验方案
研究信息

関連項目

额外MCM抗体，包括MCM2, MCM3, MCM4, MCM5, MCM6和MCM7

MCM2抗体（E-8）是一种IgG2b κ小鼠单克隆MCM2抗体，可通过WB、IP、IF和ELISA检测来自小鼠、大鼠和人的MCM2蛋白。MCM2抗体（E-8）既有非偶联型抗MCM2抗体形式，也有多种偶联型抗MCM2抗体形式，包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®偶联物。微小染色体维持（MCM）蛋白家族，包括MCM2、MCM3、MCM4（Cdc21）、MCM5（Cdc46）、MCM6（Mis5）和MCM7（Cdc47），是DNA复制的调节因子，其作用是确保细胞周期中只发生一次复制。MCM蛋白的表达在细胞生长期间增加，在G1期到S期达到峰值。每个MCM蛋白都包含一个ATP结合基序，预计可介导依赖ATP的双链DNA开放。MCM蛋白受E2F转录因子的调节，E2F转录因子诱导MCM表达，并受蛋白激酶的调节，蛋白激酶与MCM蛋白相互作用以维持细胞的复制后状态。MCM2/MCM4复合物在体外作为Cdc2/cyclin B的底物发挥作用。MCM3的裂解（可被半胱天冬酶抑制剂阻止）导致MCM复合物（至少由MCM2-6组成）在凋亡期间失活。已显示由MCM4、MCM6和MCM7组成的复合物参与DNA解旋酶活性，而MCM5参与IFN-γ诱导的Stat1α转录激活。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

MCM2 抗体 (E-8) 参考文献:

  1. ORC/Cdc6/MCM2-7复合物--调节螺旋酶装载的新力量。  |  Riera, A., et al. 2013. Cell Cycle. 12: 2155-6. PMID: 23803736
  2. 衰老期 MCM2-7 蛋白的变化  |  Suzuki, Y., et al. 2019. Genes Genet Syst. 94: 123-132. PMID: 31092751
  3. MCM2通过Akt/β-catenin途径促进子宫内膜癌细胞的干细胞特性。  |  Chu, Y., et al. 2023. Neoplasma. 70: 610-620. PMID: 38053378
  4. MCM2-7的组装、活化和解旋酶作用：从非活性MCM2-7双六聚体到活性复制叉的过渡。  |  You, Z. and Masai, H. 2024. Biology (Basel). 13: PMID: 39194567
  5. 包括参与真核生物 DNA 复制起始过程的 MCM 蛋白在内的各种假定的依赖核酸的 ATP 酶中存在一组共同的保守基序。  |  Koonin, EV. 1993. Nucleic Acids Res. 21: 2541-7. PMID: 8332451
  6. DNA 螺旋酶的活性与 MCM4, -6 和 -7 蛋白复合物有关。  |  Ishimi, Y. 1997. J Biol Chem. 272: 24508-13. PMID: 9305914
  7. 在 G2 阶段抑制蛋白激酶可使哺乳动物的 Mcm 蛋白与染色质重新结合，恢复复制能力。  |  Coverley, D., et al. 1998. Exp Cell Res. 238: 63-9. PMID: 9457057
  8. 核复制因子 MCM3 在细胞凋亡过程中的选择性蛋白水解。  |  Schwab, BL., et al. 1998. Exp Cell Res. 238: 415-21. PMID: 9473350
  9. 细胞周期和染色质结合状态依赖的人类 MCM 异六聚体复合物磷酸化。cdc2 激酶的作用。  |  Fujita, M., et al. 1998. J Biol Chem. 273: 17095-101. PMID: 9642275
  10. E2F3 的活性在细胞周期中受到调控，是诱导 S 期所必需的。  |  Leone, G., et al. 1998. Genes Dev. 12: 2120-30. PMID: 9679057
  11. 在 IFN-gamma 诱导的转录激活过程中，Stat1alpha 对 MCM5 的招募依赖于 Ser727。  |  Zhang, JJ., et al. 1998. EMBO J. 17: 6963-71. PMID: 9843502
  12. 人类 T 细胞白血病病毒 1 型税蛋白可减轻小鼠 T 细胞系对白细胞介素-2 的依赖性。  |  Iwanaga, Y., et al. 1999. J Virol. 73: 1271-7. PMID: 9882331

订购信息

产品名称产品编号规格价格数量收藏夹

MCM2 抗体 (E-8)

sc-373702
200 µg/ml
RMB2377.00

MCM2 (E-8): m-IgGκ BP-HRP 套装

sc-522465
200 µg Ab, 40 µg BP
RMB2662.00

MCM2 (E-8): m-IgG2b BP-HRP 套装

sc-549035
200 µg Ab; 10 µg BP
RMB2662.00

MCM2 抗体 (E-8) AC

sc-373702 AC
500 µg/ml, 25% agarose
RMB3129.00

MCM2 抗体 (E-8) HRP

sc-373702 HRP
200 µg/ml
RMB2377.00

MCM2 抗体 (E-8) FITC

sc-373702 FITC
200 µg/ml
RMB2482.00

MCM2 抗体 (E-8) PE

sc-373702 PE
200 µg/ml
RMB2580.00

MCM2 抗体 (E-8) Alexa Fluor® 488

sc-373702 AF488
200 µg/ml
RMB2685.00

MCM2 抗体 (E-8) Alexa Fluor® 546

sc-373702 AF546
200 µg/ml
RMB2685.00

MCM2 抗体 (E-8) Alexa Fluor® 594

sc-373702 AF594
200 µg/ml
RMB2685.00

MCM2 抗体 (E-8) Alexa Fluor® 647

sc-373702 AF647
200 µg/ml
RMB2685.00

MCM2 抗体 (E-8) Alexa Fluor® 680

sc-373702 AF680
200 µg/ml
RMB2685.00

MCM2 抗体 (E-8) Alexa Fluor® 790

sc-373702 AF790
200 µg/ml
RMB2685.00

MCM2 (E-8) 中和勝肽

sc-373702 P
100 µg/0.5 ml
RMB511.00

What application is the blocking peptide sc-373702 P appropriate for?

Asked by: DefinitelyNotMatt
Thank you for your question. The blocking peptide is intended for use as a negative control, by pre-adsorbing the mouse monoclonal antibody against the antigen. For full protocol details, please contact our Technical Services Department or view our online protocol here: https://www.scbt.com/scbt/resources/protocols/peptide-neutralization
Answered by: Technical Support
Date published: 2017-02-24
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Rated 4 out of 5 by from Good antibodyAntibody signal is good and clear upregulation is seen after x-ray irradiation, as expected.
Date published: 2021-07-28
Rated 5 out of 5 by from Stains specificallySteins specifically, colocalizes well with the nucleus. Minor cytoplasm staining detected. Verified specificity with blocking peptide and non dividing resting cells. 647 pairs well with 594 costaining with two different ABs. The typical bleedthrough of fluorescence 647 to 594 is 0, and 594 to 647 channel is only 5-10%. Excellent AB overall.
Date published: 2019-04-15
Rated 3 out of 5 by from MCM2 AbAntibody works for WB. But signal is quite weak. Protein was weakly detected at dilution 1:100 using HCT116 whole cell lysates. Band corresponding to MCM2 protein was observed around 110-120kDa.
Date published: 2018-07-23
Rated 5 out of 5 by from good featuresgood 基本没有杂带，牛奶封闭，一抗过夜，二抗牛奶室温两小时，30ug细胞蛋白，跑出条带如下图所示
Date published: 2018-03-11
Rated 5 out of 5 by from Works well for western blotWorks well for western of whole cell extracts at 1:1000 in 5% milk
Date published: 2018-01-16
Rated 5 out of 5 by from a fantastic antibody for WBThis antibody generates a clear and strong signal at the predicted position on the WB (1:500 dilution, 40ug HeLa extract).
Date published: 2017-09-18
Rated 5 out of 5 by from Good antibody!We got specific signal for MCM2 @ about 130kd in WB test.
Date published: 2017-08-22
Rated 5 out of 5 by from High specificity24hpf，2dpf，3dpf zebrefish embryos, Western blot, A clear single target band,indicates that MCM2 Antibody (E-8) the antibody with high specificity.
Date published: 2017-04-17
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MCM2 抗体 (E-8) is rated 4.5 out of 5 by 13.
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