Date published: 2025-9-7
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MAVS Antibody (E-3) - Western Blotting - Image 286130
MAVS 抗体 (E-3): sc-166583. HeLa (A) 和 CCD-1064Sk (B) 全细胞裂解液中 MAVS 表达的 Western 印迹分析.
MAVS 抗体 (E-3): sc-166583. HeLa (A) 和 CCD-1064Sk (B) 全细胞裂解液中 MAVS 表达的 Western 印迹分析.

MAVS 抗体 (E-3): sc-166583

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说明书
  • MAVS 抗体 E-3 是小鼠单克隆 IgG2a κ，MAVS抗体, 在111篇文献中引用，规格为200 µg/ml
  • 针对氨基酸1-135的抗原，在human起源的MAVS的N-terminal细胞质域内
  • MAVS 抗体 (E-3) 推荐用于WB, IP, IF, IHC(P) 和 ELISA，检测human 来源的 MAVS
  • 抗MAVS抗体（E-3）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取MAVS (E-3): sc-166583的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP, m-IgG2a BP-HRP 和 m-IgGκ BP-HRP 是 MAVS 抗体 (E-3) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与MAVS 抗体 (E-3) 组合成套装提供。 (请参阅下面的订购信息)。

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描述
基因信息
蛋白序列
说明书与实验方案
研究信息

関連項目

MAVS抗体（E-3）是一种IgG2a κ小鼠单克隆MAVS抗体（也称为IPS1抗体或VISA抗体），可通过WB、IP、IF、IHC（P）和ELISA检测人源MAVS蛋白。MAVS抗体（E-3）可提供非偶联抗MAVS抗体形式以及多种偶联形式的抗MAVS抗体，包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®偶联物。MAVS（线粒体抗病毒信号蛋白），也称为IPS1、KIAA1271、VISA或CARDIF，是一种540个氨基酸的蛋白质，含有一个CARD结构域和几个跨膜结构域，定位于线粒体外膜。MAVS在全身表达，在肝脏、心脏、胎盘、骨骼肌和外周血白细胞中表达最高，在检测双链（ds）病毒复制的蛋白质（如RIG-I）下游起作用，是针对ds病毒感染的适当免疫反应所必需的。MAVS被认为可激活导致抗病毒细胞因子诱导的途径，并可能保护细胞免受病毒诱导的凋亡。通过降解CARD和跨膜结构域的蛋白酶复合物的切割可灭活MAVS功能，从而阻止MAVS与其他蛋白质相互作用。由于选择性剪接事件，表达了三种MAVS同工型。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

MAVS 抗体 (E-3) 参考文献:

  1. 丙型肝炎病毒蛋白酶 NS3/4A 可将线粒体抗病毒信号蛋白从线粒体上切割下来，以逃避先天性免疫。  |  Li, XD., et al. 2005. Proc Natl Acad Sci U S A. 102: 17717-22. PMID: 16301520
  2. 丙型肝炎病毒感染期间 IFN-beta 启动子刺激因子 1 的病毒和治疗控制。  |  Loo, YM., et al. 2006. Proc Natl Acad Sci U S A. 103: 6001-6. PMID: 16585524
  3. 通过 NS3 蛋白酶依赖性和非依赖性机制抑制丙型肝炎病毒感染细胞中 dsRNA 诱导的信号传导。  |  Cheng, G., et al. 2006. Proc Natl Acad Sci U S A. 103: 8499-504. PMID: 16707574
  4. 丙型肝炎病毒 NS3-4A 蛋白质分解作用使 MAVS/IPS-1/VISA/Cardif-IKKepsilon 分子复合物与线粒体外膜分离。  |  Lin, R., et al. 2006. J Virol. 80: 6072-83. PMID: 16731946
  5. TRAF3 和 Cardif 之间的直接特异性相互作用对抗病毒反应的调节。  |  Saha, SK., et al. 2006. EMBO J. 25: 3257-63. PMID: 16858409
  6. 嗜肺军团菌通过 IPS-1 和 IRF3 在肺上皮细胞中诱导 IFNbeta，IPS-1 和 IRF3 还能控制细菌的复制。  |  Opitz, B., et al. 2006. J Biol Chem. 281: 36173-9. PMID: 16984921
  7. GB 病毒 B 通过 NS3/4A 介导的适配蛋白 MAVS 分裂破坏 RIG-I 信号传导。  |  Chen, Z., et al. 2007. J Virol. 81: 964-76. PMID: 17093192
  8. 通过肠上皮细胞中的 RIG-I/IPS-1 信号激活先天性免疫防御机制。  |  Hirata, Y., et al. 2007. J Immunol. 179: 5425-32. PMID: 17911629
  9. 鉴定干扰 RIGI/MAVS 信号通路的 MAVS 剪接变体。  |  Lad, SP., et al. 2008. Mol Immunol. 45: 2277-87. PMID: 18207245
  10. 乳酸通过靶向 MAVS 天然抑制 RLR 信号转导  |  Zhang, W., et al. 2019. Cell. 178: 176-189.e15. PMID: 31155231
  11. MAVS整合了葡萄糖代谢和RIG-I样受体信号传导。  |  He, QQ., et al. 2023. Nat Commun. 14: 5343. PMID: 37660168
  12. 表观遗传学扰动后CPT1A诱导促进MAVS的棕榈酰化和激活，从而增强抗肿瘤免疫。  |  Zhang, G., et al. 2023. Mol Cell. 83: 4370-4385.e9. PMID: 38016475

订购信息

产品名称产品编号规格价格数量收藏夹

MAVS 抗体 (E-3)

sc-166583
200 µg/ml
RMB2377.00

MAVS (E-3): m-IgG Fc BP-HRP 套装

sc-528998
200 µg Ab; 10 µg BP
RMB2662.00

MAVS (E-3): m-IgGκ BP-HRP 套装

sc-521667
200 µg Ab, 40 µg BP
RMB2662.00

MAVS (E-3): m-IgG2a BP-HRP 套装

sc-547231
200 µg Ab; 10 µg BP
RMB2662.00

MAVS 抗体 (E-3) AC

sc-166583 AC
500 µg/ml, 25% agarose
RMB3129.00

MAVS 抗体 (E-3) HRP

sc-166583 HRP
200 µg/ml
RMB2377.00

MAVS 抗体 (E-3) FITC

sc-166583 FITC
200 µg/ml
RMB2482.00

MAVS 抗体 (E-3) PE

sc-166583 PE
200 µg/ml
RMB2580.00

MAVS 抗体 (E-3) Alexa Fluor® 488

sc-166583 AF488
200 µg/ml
RMB2685.00

MAVS 抗体 (E-3) Alexa Fluor® 546

sc-166583 AF546
200 µg/ml
RMB2685.00

MAVS 抗体 (E-3) Alexa Fluor® 594

sc-166583 AF594
200 µg/ml
RMB2685.00

MAVS 抗体 (E-3) Alexa Fluor® 647

sc-166583 AF647
200 µg/ml
RMB2685.00

MAVS 抗体 (E-3) Alexa Fluor® 680

sc-166583 AF680
200 µg/ml
RMB2685.00

MAVS 抗体 (E-3) Alexa Fluor® 790

sc-166583 AF790
200 µg/ml
RMB2685.00

Can this antibody used to detect the aggregation of the MAVS when it is activated during the antiviral response in the cells?

Asked by: Mihai Sularea
Thank you for your question. Please contact our technical service team to discuss your experimental setup and advice on whether the antibody is suitable for your planned application.
Answered by: Tech Support Europe
Date published: 2022-09-05

During my experiments with viral infection I have observed two bands just in my infected cells (microglias), at 75 and a bigger one. What could be the cause of this? thank you

Asked by: Catarina
Thank you for your question. Please reach out to our technical service team directly so we can look into your experiment. Thanks!
Answered by: Tech Support Europe
Date published: 2022-09-05

Can this antibody be used for IF in lung tissus (costaining)?

Asked by: Yara S
Thank you for your question. MAVS Antibody (E-3) is recommended for detection of MAVS of human origin by WB, IP, IF, IHC(P) and ELISA, including human lung tissues.
Answered by: Tech Support Europe
Date published: 2021-01-14

can this antibody be used for WB in mouse cell line?

Asked by: MarX
Thank you for your question. We have not yet tested this antibody in mouse samples, and so it is currently only recommended for detection of human cells and tissues. Please check back for updates!
Answered by: Tech Service
Date published: 2019-11-04

Can this antibody be used in IHC with paraffin-embedded samples?

Asked by: AbPolly
Thank you for your question. Yes, this MAVS antibody sc-166583 has been validated for use in IHC with paraffin-embedded sections. We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support 8
Date published: 2017-01-04
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Rated 5 out of 5 by from Anti-MAVS (E-3) antibodyThe mouse monoclonal Anti-MAVS (E-3) was working very well in our Western Blotting.
Date published: 2023-10-13
Rated 5 out of 5 by from excellentThere is expected band in A549 cells when use 1/200X dilution.
Date published: 2022-07-02
Rated 5 out of 5 by from A good antibody against MAVSThis antibody works in Western blot well. It can detect endogenous protein level.
Date published: 2019-12-03
Rated 4 out of 5 by from Worked well if used in milkUsed at 1:1000 dilution in PBST gave very dirty blot, but when used in PBST 5% milk gave 1 clear band for MAVS in A549 and HaCaT cell lysate samples
Date published: 2017-10-30
Rated 5 out of 5 by from Good results in WBWe used this antibody for KO confirmation of MAVS in human BLaER1 cells (via CRISPR/Cas9). Single band at about 75 kDa. 1:1000 dilution. works fine!
Date published: 2017-10-09
Rated 5 out of 5 by from Worked great with IFClear staining of mitochondrial MAVS at 1:250 dilution at RT. Highly recommented for IF work.
Date published: 2017-09-30
Rated 5 out of 5 by from absolutely great AbThis antibody works great on my samples and showed great staining. With my mitochondrial staining, I can easily see whethere there is MAVS and how much MAVS are present.
Date published: 2017-08-21
Rated 5 out of 5 by from 功能很出色用了人源的MAVS抗体来检测CV细胞中的ＭＡＶＳ蛋白，等到了比较好的结果，出现的条带很清晰，对于此抗体推荐使用
Date published: 2017-08-15
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MAVS 抗体 (E-3) is rated 4.8 out of 5 by 20.
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