Date published: 2026-10-26

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MAP-2 Antibody (A-8) - Immunohistochemistry - Image 157167
MAP-2 抗体 (A-8): sc-74422. 对福尔马林固定, 石蜡包埋的人脑组织进行免疫过氧化物酶染色, 显示神经元细胞的神经髓和细胞质染色.
MAP-2 抗体 (A-8): sc-74422. 对福尔马林固定, 石蜡包埋的人脑组织进行免疫过氧化物酶染色, 显示神经元细胞的神经髓和细胞质染色.

MAP-2 抗体 (A-8): sc-74422

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说明书
  • MAP-2抗体(A-8)是小鼠单克隆IgG2a κ, 在13篇文献中引用，规格为200 µg/ml
  • 免疫human物种的MAP-2的氨基酸1-300
  • 推荐用于 mouse, rat 和 human 来源的MAP-2 WB, IP, IF, IHC(P) 和 ELISA检测
  • 看 MAP-2 (A-4): sc-74421，可查看有标记的 MAP-2 抗体，包括AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 和 790
  • 2a BP-HRP">m-IgG2a BP-HRP是MAP-2 Antibody (A-8) 适用于 WB 和 IHC(P) 应用。 的首选辅助检测试剂。该试剂现与MAP-2 Antibody (A-8) 打包提供（请参阅下面的订购信息）。
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相关产品
描述
基因信息
蛋白序列
说明书与实验方案
研究信息

関連項目

附加MAP抗体，包括MAP-1B, MAP-2, MAP-4, MAP-6D1和MAP-1S

微管是细胞骨架网络的主要组成部分，与微管相关蛋白（MAP）相互作用。微管相关蛋白可分为结构和动态两类。结构微管相关蛋白包括MAP-1A、MAP-1B、MAP-2A、MAP-2B和MAP-2C，可刺激微管蛋白组装，增强微管稳定性，影响细胞内微管的空间分布。MAP-1和MAP-2均被认为是通过抑制微管的动态不稳定性来参与微管解聚的。MAP蛋白对微管动态不稳定的抑制被认为与MAP的磷酸化有关。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

MAP-2 抗体 (A-8) 参考文献:

  1. 微管相关蛋白2（MAP-2）在神经元生长、可塑性和变性中的作用。  |  Johnson, GV. and Jope, RS. 1992. J Neurosci Res. 33: 505-12. PMID: 1484385
  2. MAP-2在人类腺垂体和垂体腺瘤中的表达。一项免疫组织化学研究。  |  Rotondo, F., et al. 2005. Pituitary. 8: 75-9. PMID: 16195780
  3. 重复经颅磁刺激对大鼠脑缺血再灌注损伤后三磷酸腺苷含量和微管相关蛋白-2表达的影响。  |  Feng, HL., et al. 2008. Chin Med J (Engl). 121: 1307-12. PMID: 18713553
  4. 钙网蛋白和微管相关蛋白-2（MAP-2）免疫组化在赫氏包虫病诊断中的应用  |  Yang, WI. and Oh, JT. 2013. J Pediatr Surg. 48: 2112-7. PMID: 24094966
  5. 微管相关蛋白MAP-1和MAP-2的微异质性以及单个亚组分的差异性磷酸化。  |  Herrmann, H., et al. 1985. J Biol Chem. 260: 5797-803. PMID: 2985613
  6. 响应面法优化欧斑木多糖MAP-2的提取工艺并评估其作为α-葡萄糖苷酶抑制剂的潜力。  |  Yang, Y., et al. 2022. Foods. 11: PMID: 36360143
  7. 5-HT1A激动剂和地塞米松逆转了成年大鼠脑中对对氯苯丙胺引起的MAP-2和突触素免疫反应的丧失。  |  Azmitia, EC., et al. 1995. Brain Res. 677: 181-92. PMID: 7552242
  8. 被动回避训练可增强大鼠大脑皮层神经元中乙酰胆碱受体和共表达的PKC伽马和MAP-2的免疫反应性。  |  Van der Zee, EA., et al. 1994. Cereb Cortex. 4: 376-90. PMID: 7950310
  9. 新生小鼠触须毛囊去除后小鼠桶状区域的树突状模式：MAP-2免疫组织化学。  |  Majewska, B. and Skangiel-Kramska, J. 1996. Acta Neurobiol Exp (Wars). 56: 561-6. PMID: 8768307
  10. 通过大鼠局灶性脑缺血后微管相关蛋白 2、生长相关蛋白 43 和细胞周期蛋白 D1 的免疫反应识别神经元损伤和可塑性  |  Li, Y., et al. 1998. Stroke. 29: 1972-80; discussion 1980-1. PMID: 9731626

订购信息

产品名称产品编号规格价格数量收藏夹

MAP-2 抗体 (A-8)

sc-74422
200 µg/ml
CNY2422.00

MAP-2 (A-8): m-IgG2a BP-HRP 套装

sc-546009
200 µg Ab; 10 µg BP
CNY2715.00

I am using MAP-2 (A-8): sc-74422 monoclonal antibody to stain paraffin embedded tissue. Do you recommend antigen retrieval when using this antibody?

Asked by: AbPolly
We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2020-08-11
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Rated 5 out of 5 by yuzaij from observed target bandRat brian tissue extracts (50ug),1:800 diluted the primary, and 1:5000 diluted the secondary,The WB result is expected bands at 280kDa.
Date published: 2017-07-02
Rated 5 out of 5 by laoxu from worked good qualityI tried different antibody dilutions and to optimize it, but I always get the same (very strong) bands from 1:500 to 1:800 (Hela cell).
Date published: 2017-07-01
Rated 5 out of 5 by HF from Positive signal in WB in mouse transfectedPositive signal in WB in mouse transfected 293T whole cell lysates. -SCBT QC
Date published: 2015-03-19
Rated 5 out of 5 by ET from Excellent immunoperoxidase neuropil and cytoplasmicExcellent immunoperoxidase neuropil and cytoplasmic staining in formalin fixed, paraffin-embedded human brain tissue. -SCBT QC
Date published: 2015-03-16
Rated 5 out of 5 by MS from Robust signal in WB in human transfectedRobust signal in WB in human transfected 293T whole cell lysates. -SCBT QC
Date published: 2015-01-10
Rated 5 out of 5 by FL from Worked well in WB in mouse and rat brainWorked well in WB in mouse and rat brain tissue extracts. -SCBT QC
Date published: 2015-01-08
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MAP-2 抗体 (A-8) is rated 5.0 out of 5 by 6.
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