Date published: 2025-9-7
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MAP-2 Antibody (A-4) - Western Blotting - Image 374293
MAP-2 抗体 (A-4): sc-74421. 非转染: sc-117752 (A) 和小鼠 MAP-2 转染: sc-121505 (B) 全细胞裂解液中 MAP-2 表达的近红外 western 印迹分析. 用 UltraCruz® 阻断试剂: sc-516214 阻断. 所用检测试剂: m-IgGκ BP-CFL 680: sc-516180.
MAP-2 抗体 (A-4): sc-74421. 非转染: sc-117752 (A) 和小鼠 MAP-2 转染: sc-121505 (B) 全细胞裂解液中 MAP-2 表达的近红外 western 印迹分析. 用 UltraCruz® 阻断试剂: sc-516214 阻断. 所用检测试剂: m-IgGκ BP-CFL 680: sc-516180.

MAP-2 抗体 (A-4): sc-74421

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说明书
  • MAP-2 抗体 A-4 是小鼠单克隆 IgG1 κ，MAP-2抗体, 在66篇文献中引用，规格为200 µg/ml
  • 免疫human物种的MAP-2的氨基酸1-300
  • MAP-2 抗体 (A-4) 推荐用于 WB, IP, IF, IHC(P) 和 ELISA，检测mouse, rat 和human 来源的 MAP-2
  • 抗MAP-2抗体（A-4）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取MAP-2 (A-4): sc-74421的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP, m-IgG1 BP-HRP 和 m-IgGκ BP-HRP 是 MAP-2 抗体 (A-4) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与MAP-2 抗体 (A-4) 组合成套装提供。 (请参阅下面的订购信息)。

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相关产品
描述
基因信息
蛋白序列
说明书与实验方案
研究信息

関連項目

此外 MAP 抗体包括 MAP-1B, MAP-2, MAP-4, MAP-6D1 和 MAP-1S

MAP-2抗体（A-4）是一种小鼠单克隆IgG1 kappa轻链抗体，可通过蛋白质印迹（WB）、免疫沉淀（IP）、免疫荧光（IF）、石蜡包埋切片免疫组织化学（IHCP）和酶联免疫吸附测定（ELISA）检测小鼠、大鼠和人类MAP-2蛋白。MAP-2 (A-4) 抗体有非结合型和多种结合型，包括琼脂糖、辣根过氧化物酶 (HRP)、藻红蛋白 (PE)、异硫氰酸荧光素 (FITC) 和多种 Alexa Fluor® 结合物。MAP-2蛋白在稳定微管方面发挥着关键作用，微管是细胞骨架的重要组成部分，提供结构支撑并促进细胞内运输。MAP-2主要位于神经元细胞体和树突中，对于维持神经元结构的完整性和调节微管动态至关重要。MAP-2结合微管蛋白并促进微管组装的能力对于神经元的发育和功能尤为重要，有助于确保细胞形状和信号传导通路正常。此外，MAP-2还会经历多种翻译后修饰，包括磷酸化，这会影响其与其他蛋白的结合亲和力和功能相互作用，从而影响微管的稳定性和动态性。抗MAP-2抗体（A-4）在多种应用中的多功能性使其成为研究人员在各种生物学背景下研究细胞骨架动态和神经元功能的宝贵工具。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

MAP-2 抗体 (A-4) 参考文献:

  1. 了解微管功能和细胞组织：综述。  |  MacRae, TH. 1992. Biochem Cell Biol. 70: 835-41. PMID: 1297349
  2. 免疫化学证据表明，磷酸化 MAP5 (MAP1B) 片段与阿尔茨海默病的神经纤维缠结结合。  |  Hasegawa, M., et al. 1990. Neuron. 4: 909-18. PMID: 2361014
  3. 活体大鼠脑中微管相关蛋白2的多种磷酸盐。  |  Tsuyama, S., et al. 1987. J Biol Chem. 262: 10886-92. PMID: 3611094
  4. 增加肝脏白细胞介素-1、花生四烯酸和活性氧，可增强肠道半胱氨酸蛋白酶对小鼠曼氏血吸虫感染的保护作用。  |  Tallima, H. and El Ridi, R. 2023. PLoS Negl Trop Dis. 17: e0011164. PMID: 36920999
  5. 微管相关蛋白2与肌动蛋白丝的相互作用。  |  Sattilaro, RF. 1986. Biochemistry. 25: 2003-9. PMID: 3707926
  6. 微管蛋白相关蛋白在微管成核和伸长中的作用  |  Murphy, DB., et al. 1977. J Mol Biol. 117: 33-52. PMID: 599568
  7. 微管相关蛋白在控制微管组装中的作用  |  Maccioni, RB. and Cambiazo, V. 1995. Physiol Rev. 75: 835-64. PMID: 7480164
  8. 脑微管相关蛋白对体内微管动态不稳定性的调节  |  Dhamodharan, R. and Wadsworth, P. 1995. J Cell Sci. 108 (Pt 4): 1679-89. PMID: 7615685
  9. 丝裂原活化蛋白激酶信号转导途径。  |  Davis, RJ. 1993. J Biol Chem. 268: 14553-6. PMID: 8325833
  10. 微管相关蛋白 MAP1B 和 MAP2 对微管动力学调控的差异。  |  Vandecandelaere, A., et al. 1996. Cell Motil Cytoskeleton. 35: 134-46. PMID: 8894283
  11. 大鼠脑中MAP 2激酶的细胞表达。  |  Jacobowitz, DM., et al. 1996. Histochem Cell Biol. 106: 303-10. PMID: 8897071
  12. 微管相关蛋白和体外刺激微管蛋白组装。  |  Sloboda, RD., et al. 1976. Biochemistry. 15: 4497-505. PMID: 974072

订购信息

产品名称产品编号规格价格数量收藏夹

MAP-2 抗体 (A-4)

sc-74421
200 µg/ml
RMB2377.00

MAP-2 (A-4): m-IgG Fc BP-HRP 套装

sc-528663
200 µg Ab; 10 µg BP
RMB2662.00

MAP-2 (A-4): m-IgGκ BP-HRP 套装

sc-521108
200 µg Ab, 40 µg BP
RMB2662.00

MAP-2 (A-4): m-IgG1 BP-HRP 套装

sc-543058
200 µg Ab; 20 µg BP
RMB2662.00

MAP-2 抗体 (A-4) AC

sc-74421 AC
500 µg/ml, 25% agarose
RMB3129.00

MAP-2 抗体 (A-4) HRP

sc-74421 HRP
200 µg/ml
RMB2377.00

MAP-2 抗体 (A-4) FITC

sc-74421 FITC
200 µg/ml
RMB2482.00

MAP-2 抗体 (A-4) PE

sc-74421 PE
200 µg/ml
RMB2580.00

MAP-2 抗体 (A-4) Alexa Fluor® 488

sc-74421 AF488
200 µg/ml
RMB2685.00

MAP-2 抗体 (A-4) Alexa Fluor® 546

sc-74421 AF546
200 µg/ml
RMB2685.00

MAP-2 抗体 (A-4) Alexa Fluor® 594

sc-74421 AF594
200 µg/ml
RMB2685.00

MAP-2 抗体 (A-4) Alexa Fluor® 647

sc-74421 AF647
200 µg/ml
RMB2685.00

MAP-2 抗体 (A-4) Alexa Fluor® 680

sc-74421 AF680
200 µg/ml
RMB2685.00

MAP-2 抗体 (A-4) Alexa Fluor® 790

sc-74421 AF790
200 µg/ml
RMB2685.00

Can MAP-2 (A-4): sc-74421 monoclonal antibody be used to stain formalin-fixed, paraffin-embedded (FFPE) tissue sections?

Asked by: jenniferc
Thank you for your question. Yes, MAP-2 (A-4): sc-74421 monoclonal antibody has been validated for use in IHC with paraffin-embedded sections. We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support 2
Date published: 2017-01-11

What secondary is best to use with MAP-2 (A-4): sc-74421 monoclonal antibody?

Asked by: jenniferc
Thank you for your question. While most anti-mouse IgG secondary antibodies are appropriate for use with MAP-2 (A-4): sc-74421, we recommend using one our exclusive Mouse IgG Binding Proteins as a secondary detection reagent. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Answered by: Technical Support 2
Date published: 2017-01-11
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Rated 5 out of 5 by from Excellent!It beautifully works very well in IF of mouse primary neurons!
Date published: 2025-01-27
Rated 3 out of 5 by from Buoi risultati in immunofluorescenzaHo ottenuto buoni risultati in immunofluorescenza testando questo anticorpo su campioni (ratto) fissati in paraformaldeide 4%
Date published: 2022-07-05
Rated 5 out of 5 by from Worked as advertisedI bought this product because I saw a previous review that said this antibody works in ICC on neurons derived from iPSCs. It does. I followed the recommended protocol, 1:200 dilution.
Date published: 2020-03-11
Rated 3 out of 5 by from Used to stain dendritesUsed at 1:500 to stain dendrites in Immunocytochemistry. May stain axons
Date published: 2019-10-05
Rated 5 out of 5 by from Excellent functionThe MAP-2 Antibody (A-4): sc-74421 provides good data for a multitude of applications including WB, IP, IF, and IHC(P). This antibody has also been cited several times for further verification.
Date published: 2019-02-20
Rated 4 out of 5 by from Good antibodyThis antibody worked very well with fixed frozen mouse tissue sections for IF
Date published: 2018-10-03
Rated 3 out of 5 by from Detected multiple bandsI tested this antibody on mouse brain lysates, detected a single strong band in WB (1:200 dilution).
Date published: 2018-07-09
Rated 5 out of 5 by from Works for human NPCsWorks well for direct immunofluorescence staining of PFA-fixed human NPCs
Date published: 2018-06-06
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MAP-2 抗体 (A-4) is rated 4.5 out of 5 by 23.
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