Date published: 2025-9-11
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Macrophage Marker Antibody (25F9) - Flow Cytometry - Image 14461
巨噬细胞标记物 (25F9): sc-52698. 用巨噬细胞标记物 (25F9) 染色 U-937 细胞, 然后用 PE 结合的山羊抗小鼠 IgG: sc-3738 进行间接 FCM 分析. 黑线直方图代表同型对照, 正常小鼠 IgG1: sc-3877.
Macrophage Marker Antibody (25F9) - Flow Cytometry - Image 14461
巨噬细胞标记物 (25F9): sc-52698. 用巨噬细胞标记物 (25F9) 染色 U-937 细胞, 然后用 PE 结合的山羊抗小鼠 IgG: sc-3738 进行间接 FCM 分析. 黑线直方图代表同型对照, 正常小鼠 IgG1: sc-3877.
巨噬细胞标记物 (25F9): sc-52698. 用巨噬细胞标记物 (25F9) 染色 U-937 细胞, 然后用 PE 结合的山羊抗小鼠 IgG: sc-3738 进行间接 FCM 分析. 黑线直方图代表同型对照, 正常小鼠 IgG1: sc-3877.

Macrophage Marker 抗体 (25F9): sc-52698

4.0(1)
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说明书
  • Macrophage Marker 抗体 25F9 是小鼠单克隆 IgG1，提供 100 µg/ml
  • 推荐用于 human 和 porcine 来源的mature macrophages WB, IF, IHC(P) 和 FCM检测
  • 看 Macrophage Marker (MAC387): sc-66204，可查看有标记的 Macrophage Marker 抗体，包括AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 和 790
  • 目前，我们还没有完成Macrophage Marker 抗体 (25F9)的首选二抗检测试剂的鉴定。这项工作正在进行中。

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    関連項目

    此外 Macrophage 抗体包括 和 Monocyte Markers

    血液由固体成分和液体成分组成，固体成分包括红细胞、白细胞和血小板，液体成分称为血浆，是蛋白质和盐的缓冲溶液。先天性和适应性免疫反应依赖于白细胞的功能，白细胞是有核的白细胞，可以破坏入侵细胞并清除碎片。白细胞也称为多形核白细胞，包括粒细胞、单核细胞和肥大细胞前体。巨噬细胞是组织定位的分化细胞，来自循环单核细胞。与循环中性粒细胞一起，巨噬细胞是吞噬细胞，吞噬抗体包被的病原体，随后在细胞内囊泡中降解。组织定位的巨噬细胞可以靶向一系列细菌病原体，而不需要预先暴露。

    仅限研究使用。不适用于诊断和治疗用途。

    Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

    LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

    Macrophage Marker 抗体 (25F9) 参考文献:

    1. LRP5：一种新型抗炎巨噬细胞标记物，可有效调节迁移和吞噬作用。  |  Ma, Y. 2016. J Mol Cell Cardiol. 91: 61-2. PMID: 26739212
    2. F4/80作为巨噬细胞的主要标志物：腹膜和脾脏的案例。  |  Dos Anjos Cassado, A. 2017. Results Probl Cell Differ. 62: 161-179. PMID: 28455709
    3. 巨噬细胞标志物易位蛋白（TSPO）在促炎性"M1"人巨噬细胞中下调。  |  Narayan, N., et al. 2017. PLoS One. 12: e0185767. PMID: 28968465
    4. M2巨噬细胞标志物CD206：急性髓细胞白血病的新型预后指标。  |  Xu, ZJ., et al. 2020. Oncoimmunology. 9: 1683347. PMID: 32002295
    5. 前庭神经鞘瘤体积和肿瘤生长与巨噬细胞标记表达相关。  |  Leisz, S., et al. 2022. Cancers (Basel). 14: PMID: 36139588
    6. 验证巨噬细胞标记基因（MMGS）在肺腺癌预后和免疫治疗反应中的有效性。  |  Song, P., et al. J Immunother. 46: 205-215. PMID: 37220007
    7. 通过单细胞和整体RNA测序的综合分析，基于巨噬细胞标记基因的新型特征的鉴定和验证，可用于预测肾透明细胞癌的预后和药物反应。  |  Chen, X., et al. 2024. Aging (Albany NY). 16: 5676-5702. PMID: 38517387
    8. SFRP1可降低乳腺组织中WNT介导的M2巨噬细胞标记物的表达。  |  Gregory, KJ., et al. 2024. Cancer Immunol Immunother. 73: 86. PMID: 38554160
    9. 生物信息学分析揭示了巨噬细胞标记基因在胃癌中的预后意义。  |  Li, Z., et al. 2024. Front Biosci (Landmark Ed). 29: 172. PMID: 38812299
    10. 鉴定巨噬细胞标记基因以评估肝细胞癌的免疫浸润和治疗反应。  |  Li, T., et al. 2024. Heliyon. 10: e31881. PMID: 38845876

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    Macrophage Marker 抗体 (25F9)

    sc-52698
    100 µg/ml
    		RMB2377.00

    Macrophage maker antibody 25F9 is described as a "marker for mature macrophages" made against a cell lysate. This is a monoclonal antibody with a discrete antigen. Does it detect a specific protein? If so, what is it?

    Asked by: PaulS
    Thank you for your question. It would be helpful if you could call us, allowing for a more interactive discussion of this and other related questions.
    Answered by: Technical Support
    Date published: 2021-07-22

    is this offered biotinylated?

    Asked by: sallie
    Thank you for your question. It would be helpful if you could call us, allowing for a more interactive discussion of this and other related questions.
    Answered by: Technical Support
    Date published: 2021-07-22

    Could you please help me out in deciding that i can use this antibody to detect macrophage marker 25F9 on bovine macrophages, as this is a monoclonal antibody from human origin. Thus, if you could please let me know its cross reactivity.

    Asked by: Vivek30
    Thank you for your question. Macrophage Marker Antibody (25F9): sc-52698 is only recommended for detection of mature macrophages of human and porcine origin by WB, IF, IHC(P) and FCM. We do not have any data indicating cross-reactivity with bovine samples.
    Answered by: Technical Support Europe
    Date published: 2020-10-21

    Does this ab distinguish between suspension U937 cells and U937s differentiated into macrophages?

    Asked by: Ms Lisa
    Thank you for your question. We are not aware of any publications citing use of our Macrophage Marker Antibody (25F9): sc-52698 in U-937 cells for this purpose so we can not answer this question. The 25F9 clone recognizes a protein of 86 kDa on the cell surface and within the cytoplasm of mature macrophages. The antibody is associated with fully differentiated tissue macrophages both in normal and in diseased tissues, independently of the presence or absence of inflammation. The antigen is absent on freshly isolated monocytes and other blood cells.
    Answered by: Technical Support
    Date published: 2017-03-17

    How can Macrophage Marker (25F9): sc-52698 mouse monoclonal antibody be used for double or triple staining, if the other primary antibodies are also raised in mouse?

    Asked by: AbPolly
    In this case, we recommend using a directly conjugated mouse monoclonal primary antibody. This antibody is available conjugated to phycoerythrin (sc-52698 PE) or fluoroscein (sc-52698 FITC).
    Answered by: Technical Support
    Date published: 2017-02-25
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    Rated 4 out of 5 by from Good indirect FCM analysis of UGood indirect FCM analysis of U-937 cells stained with Macrophage marker . -SCBT QC
    Date published: 2013-09-19
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    Macrophage Marker 抗体 (25F9) is rated 4.0 out of 5 by 1.
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