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Macrophage Marker 抗体 (MAC387): sc-66204

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说明书
  • Macrophage Marker 抗体 MAC387 是小鼠单克隆 IgG1 κ，Macrophage Marker抗体, 在24篇文献中引用，规格为200 µg/ml
  • 针对human来源的外周血单核细胞成分
  • Macrophage Marker 抗体 (MAC387) 推荐用于 WB, IP, IF, IHC(P), FCM 和 ELISA，检测mouse, rat 和human 来源的 macrophages
  • 抗Macrophage Marker抗体（MAC387）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取Macrophage Marker (MAC387): sc-66204的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • 目前，我们还没有完成Macrophage Marker 抗体 (MAC387)的首选二抗检测试剂的鉴定。这项工作正在进行中。

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说明书与实验方案
研究信息

関連項目

此外 Macrophage 抗体包括 和 Monocyte Markers

Macrophage Marker 抗体（MAC387）是一种小鼠单克隆 IgG1 kappa 轻链抗体，可通过免疫印迹（WB）、免疫沉淀（IP）、免疫荧光（IF）、石蜡包埋切片免疫组织化学（IHCP）、流式细胞术（FCM）和酶联免疫吸附试验（ELISA）检测小鼠、大鼠和人样本中的 Macrophage Marker 蛋白。抗巨噬细胞标志物抗体（MAC387）有非共轭型和与琼脂糖、辣根过氧化物酶（HRP）、藻红蛋白（PE）、异硫氰酸荧光素（FITC）和多种 Alexa Fluor® 染料共轭的两种形式，为各种实验应用提供了灵活性。巨噬细胞标记蛋白 (MAC387) 在免疫系统功能中发挥着关键作用，特别是在病原体和碎片的识别和清除方面，它能维持组织的平衡并触发免疫反应。表达 "巨噬细胞标记蛋白 "的巨噬细胞由循环中的单核细胞发育而来，作为主要的感染防御者驻留在组织中。巨噬细胞标记蛋白能快速吞噬和降解病原体，而无需事先致敏，因此这种蛋白是抵御各种感染病原体的先天免疫所必需的。巨噬细胞标志蛋白与特定信号分子的相互作用增强了免疫细胞之间的交流，协调了免疫反应，凸显了该蛋白在先天免疫和整体机体健康中的重要作用。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

Macrophage Marker 抗体 (MAC387) 参考文献:

  1. 人类成骨细胞样细胞在体外吞噬金属颗粒并表达巨噬细胞标记 CD68。  |  Heinemann, DE., et al. 2000. J Bone Joint Surg Br. 82: 283-9. PMID: 10755442
  2. 肠炎沙门氏菌血清型普拉伦沙门氏菌在鸡的脾脏巨噬细胞和生殖道中持续存在，无病携带。  |  Wigley, P., et al. 2001. Infect Immun. 69: 7873-9. PMID: 11705970
  3. 卡波西氏肉瘤病灶中的梭形细胞和卡波西氏肉瘤衍生肿瘤细胞表达成纤维细胞/巨噬细胞标记物1B10。  |  Simonart, T., et al. 2002. J Cutan Pathol. 29: 72-8. PMID: 12150136
  4. 单核细胞和巨噬细胞的异质性。  |  Gordon, S. and Taylor, PR. 2005. Nat Rev Immunol. 5: 953-64. PMID: 16322748
  5. 单核吞噬细胞系统。  |  Hume, DA. 2006. Curr Opin Immunol. 18: 49-53. PMID: 16338128
  6. 调节破骨细胞的分化  |  Roodman, GD. 2006. Ann N Y Acad Sci. 1068: 100-9. PMID: 16831910
  7. 人类外周血嗜酸性粒细胞型集落的异质性：嗜碱性粒细胞-嗜酸性粒细胞共同祖先的证据。  |  Denburg, JA., et al. 1985. Blood. 66: 312-8. PMID: 2410064
  8. 克隆人 T 细胞在被 HTLV-I 转化前后产生吞噬诱导因子和表达 4B4 抗原。  |  Margolick, JB., et al. 1988. Cell Immunol. 111: 196-203. PMID: 2827893
  9. 导致对 PPD 产生 '体外 '反应的免疫事件。  |  Scordamaglia, A., et al. 1987. Allergol Immunopathol (Madr). 15: 83-7. PMID: 3113220
  10. 人类肝脏和胃肠道巨噬细胞的免疫组织化学特征：正常和患病组织中CD4、HLA-DR、OKM1和成熟巨噬细胞标记物25F9的表达。  |  Hume, DA., et al. 1987. J Leukoc Biol. 42: 474-84. PMID: 3500252
  11. 对视网膜外膜中的 IgG, 补体 C3, III 型胶原和巨噬细胞标记物 Ki-M7 的免疫组织化学研究。  |  Shi, Y., et al. 1996. Yan Ke Xue Bao. 12: 10-4. PMID: 9275730
  12. 单克隆抗体 KUL01 对鸡单核细胞, 巨噬细胞和间变细胞的表征。  |  Mast, J., et al. 1998. Vet Immunol Immunopathol. 61: 343-57. PMID: 9613446

订购信息

产品名称产品编号规格价格数量收藏夹

Macrophage Marker 抗体 (MAC387)

sc-66204
200 µg/ml
RMB2377.00

Macrophage Marker 抗体 (MAC387) AC

sc-66204 AC
500 µg/ml, 25% agarose
RMB3129.00

Macrophage Marker 抗体 (MAC387) HRP

sc-66204 HRP
200 µg/ml
RMB2377.00

Macrophage Marker 抗体 (MAC387) FITC

sc-66204 FITC
200 µg/ml
RMB2482.00

Macrophage Marker 抗体 (MAC387) PE

sc-66204 PE
200 µg/ml
RMB2580.00

Macrophage Marker 抗体 (MAC387) Alexa Fluor® 488

sc-66204 AF488
200 µg/ml
RMB2685.00

Macrophage Marker 抗体 (MAC387) Alexa Fluor® 546

sc-66204 AF546
200 µg/ml
RMB2685.00

Macrophage Marker 抗体 (MAC387) Alexa Fluor® 594

sc-66204 AF594
200 µg/ml
RMB2685.00

Macrophage Marker 抗体 (MAC387) Alexa Fluor® 647

sc-66204 AF647
200 µg/ml
RMB2685.00

Macrophage Marker 抗体 (MAC387) Alexa Fluor® 680

sc-66204 AF680
200 µg/ml
RMB2685.00

Macrophage Marker 抗体 (MAC387) Alexa Fluor® 790

sc-66204 AF790
200 µg/ml
RMB2685.00

Dose this antibody stain also microglia?

Asked by: Hadas
Thank you for your question. Please contact your local distributor or technical service for further assistance.
Answered by: Dallas Tech Service
Date published: 2021-04-07

Does this antibody stain all macrophages, meaning both M1 and M2? Thank you very much

Asked by: Ioannis
Thank you for your question. This Macrophage Marker Antibody (MAC387): sc-66204 does not stain all macrophages, but a subset of macrophages (PMID: 3309045).
Answered by: Technical Support
Date published: 2024-03-14

When probing tissues with this antibody, is it important that the tissues first be perfused and then post-fixed before embedding in OCT, or can I harvest the tissue, immediately snap-freeze in OCT, and then post-fix after cutting the tissue?

Asked by: nhamlin
Thank you for your question. Frozen tissue blocks may be stored at -70° C until ready for sectioning. Thaw slides at room temperature prior to fixing and staining. A link to our recommended protocol is provided below as a reference. https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-08-18

is the marker on the cell surface? thanks.

Asked by: RChu
Thank you for your question This clone recognizes an an intracytoplasmic epitope expressed by granulocytes, monocytes and by tissue macrophages. If you have any further questions, please contact Technical Service by phone, (800)-457-3801 option 2, email <[email protected]>, or by live chat directly on our website, www.scbt.com.
Answered by: Tech Service
Date published: 2017-06-23

Does this ab distinguish between suspension U937 cells and U937s that have been differentiated into macrophages? Thanks

Asked by: Ms Lisa
Thank you for your question. We are not aware of any publications citing use of our Macrophage Marker Antibody (MAC387): sc-66204 in U-937 cells so we can not answer this question. The MAC387 clone recognises the L1 or Calprotectin molecule, an intracytoplasmic antigen comprising of a 12kD alpha chain and a 14kD beta chain expressed by granulocytes, monocytes and by tissue macrophages.
Answered by: Technical Support
Date published: 2018-10-18

what is the kDa of this product sc-66204?

Asked by: Ambika
Thank you for your question. This Macrophage Marker Antibody (MAC387) is a mouse monoclonal recommended for detection of macrophages of mouse, rat and human origin. There is no standard expected molecular weight to be observed in kDa when using this antibody in Western blot, because the antibody does not detect a specific protein of a single size. Instead, it detects an antigen present in all macrophages.
Answered by: Technical Support
Date published: 2022-07-19

What is the recommended secondary reagent to be used with this mouse monoclonal primary?

Asked by: jerojero
We recommend using one our exclusive Mouse IgG Binding Proteins as a secondary detection reagent. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Answered by: Technical Support 9
Date published: 2017-01-24
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Rated 5 out of 5 by from very good antibodyI bought this antibody use for pig and rat tissue cyosections immunofluorescence stain. We got good result.
Date published: 2017-05-19
Rated 4 out of 5 by from positive cells in ducksWe detected positive cells (green signal in the picture; presumably macrophages) by IF in cryo-sections of nasal mucosa of ducks infected by the neuropathogenic schistosome Trichobilharzia regenti. Antibody dilution 1:100.
Date published: 2017-03-07
Rated 5 out of 5 by from Excellent Macrophage Marker Antibody!There are many clones to choose from as Macrophage Markers, but the MAC387 clone is proven to be versatile for many applications including WB, IF, IHC(P) and FCM. And is also proven to be reactive with a broad range of species.
Date published: 2017-01-05
Rated 5 out of 5 by from PerfectGot good results when used with the mouse retina section slide for immunohistochemistry .
Date published: 2016-12-11
Rated 5 out of 5 by from This antibody was used in a publication onThis antibody was used in a publication on rat RGCs - SCBT Publication Review
Date published: 2015-01-28
Rated 5 out of 5 by from IF publishable data with rat skeletal muscleIF publishable data with rat skeletal muscle - SCBT Publication Review
Date published: 2014-02-02
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Macrophage Marker抗体 (MAC387) is rated 4.8 out of 5 by 6.
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