Date published: 2025-9-6
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Lyn Antibody (H-6) - Western Blotting - Image 374440
Lyn 抗体 (H-6): sc-7274. 在 HeLa (A), 3T3-L1 (B), HEL 92. 1. 7 (C), K-562 (D), Raji (E) 和 A-431 (F) 全细胞裂解液中进行 Lyn 表达的 Western 印迹分析. 所用检测试剂: m-IgGκ BP-HRP: sc-516102.
Lyn 抗体 (H-6): sc-7274. 在 HeLa (A), 3T3-L1 (B), HEL 92. 1. 7 (C), K-562 (D), Raji (E) 和 A-431 (F) 全细胞裂解液中进行 Lyn 表达的 Western 印迹分析. 所用检测试剂: m-IgGκ BP-HRP: sc-516102.

Lyn 抗体 (H-6): sc-7274

4.9(17)
寫評論提問

说明书
  • Lyn 抗体 H-6 是小鼠单克隆 IgG2a κ，Lyn抗体, 在116篇文献中引用，规格为200 µg/ml
  • 特异性抗原位于human物种的Lyn的N-terminus附近的氨基酸44-63之间
  • Lyn 抗体 (H-6) 推荐用于 WB, IP, IF, IHC(P) 和 ELISA，检测mouse, rat 和human 来源的 Lyn p56/p53
  • 抗Lyn抗体（H-6）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取Lyn (H-6): sc-7274的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP 和 m-IgGκ BP-HRP 是 Lyn 抗体 (H-6) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与Lyn 抗体 (H-6) 组合成套装提供。 (请参阅下面的订购信息)。

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描述
基因信息
说明书与实验方案
研究信息

関連項目

Lyn抗体（H-6）是一种小鼠单克隆IgG2a kappa轻链抗体，可通过WB、IP、IF、IHCP和ELISA检测小鼠、大鼠和人类的Lyn蛋白。Lyn（H-6）单克隆抗体有非结合型和多种结合型，包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®结合物。Lyn蛋白是Src家族非受体酪氨酸激酶的成员，在调节细胞增殖、分化、凋亡等过程中发挥着关键作用，因此Lyn对于正常的免疫功能和造血功能至关重要。Lyn主要存在于造血组织中，对于B细胞和T细胞的发展和调节至关重要，它会影响免疫球蛋白的产生和免疫反应的信号传导通路。人类Lyn基因位于8q12染色体上，编码一种505个氨基酸的蛋白质，该蛋白质通常处于非活性状态，但在细胞分裂等细胞活动中可以被激活，这凸显了Lyn在细胞周期调节和免疫系统功能中的重要性。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

Lyn 抗体 (H-6) 参考文献:

  1. Src 家族激酶：结构与功能。  |  Tatosyan, AG. and Mizenina, OA. 2000. Biochemistry (Mosc). 65: 49-58. PMID: 10702640
  2. 白细胞中 Src 激酶介导的信号传导。  |  Korade-Mirnics, Z. and Corey, SJ. 2000. J Leukoc Biol. 68: 603-13. PMID: 11073097
  3. Src 激酶活化和功能的精选一瞥。  |  Bjorge, JD., et al. 2000. Oncogene. 19: 5620-35. PMID: 11114743
  4. 林激酶与脂质筏关联的结构-功能分析以及 Fcepsilon 受体 I 聚合后早期信号事件的启动。  |  Kovárová, M., et al. 2001. Mol Cell Biol. 21: 8318-28. PMID: 11713268
  5. 基质刚性通过具有机械响应性的 EPHA2/LYN 复合物控制上皮-间充质可塑性和肿瘤转移  |  Fattet, L., et al. 2020. Dev Cell. 54: 302-316.e7. PMID: 32574556
  6. LYN激酶通过调节c-JUN和THBS1，使间质成纤维细胞促进白血病细胞的存活。  |  Vom Stein, AF., et al. 2023. Nat Commun. 14: 1330. PMID: 36899005
  7. 常活性 Lyn 激酶可导致皮肤小血管血管炎和肝纤维化综合征。  |  de Jesus, AA., et al. 2023. Nat Commun. 14: 1502. PMID: 36932076
  8. Thy-1 介导的大鼠肥大细胞活化：Thy-1 膜微域的作用。  |  Dráberová, L., et al. 1996. Immunology. 87: 141-8. PMID: 8666426
  9. 通过 I 型 Fc epsilon 受体激活的大鼠嗜碱性粒细胞白血病细胞中 Syk 和 Lyn 蛋白酪氨酸激酶的直接相互作用。  |  Amoui, M., et al. 1997. Eur J Immunol. 27: 321-8. PMID: 9022035
  10. Lyn，一种类 src 酪氨酸激酶。  |  Hibbs, ML. and Dunn, AR. 1997. Int J Biochem Cell Biol. 29: 397-400. PMID: 9202419
  11. 深入了解 Src 激酶的功能：结构比较。  |  Williams, JC., et al. 1998. Trends Biochem Sci. 23: 179-84. PMID: 9612082

订购信息

产品名称产品编号规格价格数量收藏夹

Lyn 抗体 (H-6)

sc-7274
200 µg/ml
RMB2377.00

Lyn (H-6): m-IgG Fc BP-HRP 套装

sc-528170
200 µg Ab; 10 µg BP
RMB2662.00

Lyn (H-6): m-IgGκ BP-HRP 套装

sc-520484
200 µg Ab, 40 µg BP
RMB2662.00

Lyn 抗体 (H-6) AC

sc-7274 AC
500 µg/ml, 25% agarose
RMB3129.00

Lyn 抗体 (H-6) HRP

sc-7274 HRP
200 µg/ml
RMB2377.00

Lyn 抗体 (H-6) FITC

sc-7274 FITC
200 µg/ml
RMB2482.00

Lyn 抗体 (H-6) PE

sc-7274 PE
200 µg/ml
RMB2580.00

Lyn 抗体 (H-6) Alexa Fluor® 488

sc-7274 AF488
200 µg/ml
RMB2685.00

Lyn 抗体 (H-6) Alexa Fluor® 546

sc-7274 AF546
200 µg/ml
RMB2685.00

Lyn 抗体 (H-6) Alexa Fluor® 594

sc-7274 AF594
200 µg/ml
RMB2685.00

Lyn 抗体 (H-6) Alexa Fluor® 647

sc-7274 AF647
200 µg/ml
RMB2685.00

Lyn 抗体 (H-6) Alexa Fluor® 680

sc-7274 AF680
200 µg/ml
RMB2685.00

Lyn 抗体 (H-6) Alexa Fluor® 790

sc-7274 AF790
200 µg/ml
RMB2685.00

Lyn (H-6) 中和勝肽

sc-7274 P
100 µg/0.5 ml
RMB511.00

Does this antibody has been tested for guinea pig protein analysis (WB)?

Asked by: ricardo0714
Thank you for your question. It would be helpful if you could call us, allowing for a more interactive discussion of this and other related questions.
Answered by: Technical Support
Date published: 2021-07-22

How Lyn (H-6): sc-7274 monoclonal antibody be used for double or triple staining, if the other primary antibodies are also raised in mouse?

Asked by: jenniferc
In this case, we recommend using a directly conjugated mouse monoclonal primary antibody. Lyn (H-6): sc-7274 monoclonal antibody is currently available conjugated to phycoerythrin (sc-7274 PE), fluorescein (sc-7274 FITC), Alexa Fluor® 488 (sc-7274 AF488) or Alexa Fluor® 647 (sc-7274 AF647).
Answered by: Technical Support 3
Date published: 2017-01-10
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Rated 5 out of 5 by from Western Blot and IHC on canine samplesWorked well on dog samples; 1:500 in Western Blot and 1:200 in IHC on FFPE tissue
Date published: 2022-07-13
Rated 5 out of 5 by from Excellent antibody for westernsProbed at 1/1000X dilution for 2 hrs and used with Kappa-binding HRP and the blot turned out perfect. Excellent antibody for westerns.
Date published: 2017-06-21
Rated 5 out of 5 by from WONDERFULstrong and clean band. WB in 293T cells (Dilution: 1:1000)
Date published: 2017-04-07
Rated 5 out of 5 by from Good for WBI used this antibody for my IP WB, and it gave me very strong band
Date published: 2017-03-30
Rated 5 out of 5 by from worked goodThis antibody worked very good for WB ,got strong bands at 55kda.
Date published: 2017-02-24
Rated 4 out of 5 by from WELL BY WBIT WORKED WELL BY WB IN LAB, WILL PURCHASE IT IN THE FUTURE
Date published: 2017-02-20
Rated 5 out of 5 by from Best Lyn antibodyVery clear western blot data; strong signal with low background; also nice IHC
Date published: 2017-01-12
Rated 5 out of 5 by from 做免疫荧光很不错！两周以前买了该抗体（LYN, H-6）, 用于小鼠肺组织免疫荧光实验（抗体稀释比：1:100），效果相当不错。
Date published: 2016-12-21
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Lyn 抗体 (H-6) is rated 4.9 out of 5 by 17.
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