Date published: 2025-9-6
访问圣克鲁斯动物健康

021-6093-6350

SCBT Portrait Logo
Seach Input
联系我们
登陆账户
快速订购
购物车 (0)
LIMK-1 Antibody (H-12) - Western Blotting - Image 356924
LIMK-1 抗体 (H-12): sc-515585. U-87 MG (A), Ramos (B) 和 NRK (C) 全细胞裂解液中 LIMK-1 表达的 Western 印迹分析.
LIMK-1 抗体 (H-12): sc-515585. U-87 MG (A), Ramos (B) 和 NRK (C) 全细胞裂解液中 LIMK-1 表达的 Western 印迹分析.

LIMK-1 抗体 (H-12): sc-515585

5.0(6)
寫評論提問

说明书
  • LIMK-1抗体(H-12)是小鼠单克隆IgG1 κ, 在5篇文献中引用，规格为200 µg/ml
  • 特异性抗原位于human物种的LIMK-1的C-terminus的氨基酸624-647之间
  • 抗-LIMK-1 抗体 (H-12) 推荐用于 WB, IP, IF 和 ELISA，检测mouse, rat 和human 来源的 LIMK-1 long and short forms
  • 抗LIMK-1抗体（H-12）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取LIMK-1 (H-12): sc-515585的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP、 1 BP-HRP">m-IgG1 BP-HRP和m-IgGκ BP-HRP是LIMK-1 Antibody (H-12) 适用于 WB 应用。 的首选辅助检测试剂。这些试剂现与LIMK-1 Antibody (H-12) 打包提供（请参阅下面的订购信息）。

快捷链接

相关产品
描述
基因信息
说明书与实验方案
研究信息

関連項目

附加LIMK抗体，包括LIMK-1和LIMK-2

LIMK-1抗体（H-12）是一种小鼠单克隆IgG1 kappa轻链抗体，可通过蛋白质印迹（WB）、免疫沉淀（IP）、免疫荧光（IF）和酶联免疫吸附（ELISA）等方法检测小鼠、大鼠和人类样本中的LIMK-1。LIMK-1 (H-12) 抗体有非结合型和多种结合型可供选择，包括琼脂糖、辣根过氧化物酶 (HRP)、藻红蛋白 (PE)、异硫氰酸荧光素 (FITC) 和多种 Alexa Fluor® 结合物。LIMK-1是LIM激酶家族的一员，在调节肌动蛋白细胞骨架动态方面发挥着关键作用，而肌动蛋白细胞骨架动态对于细胞运动、形态发生和神经元分化至关重要。LIMK-1磷酸化并灭活Cofilin，后者是一种切断肌动蛋白丝的蛋白，可促进F-肌动蛋白结构的稳定。这种调节作用可维持细胞结构，并促进细胞对外部信号的反应。LIMK-1影响细胞命运决定和生长控制途径，凸显了发育过程的重要性。LIMK-1和LIMK-2的不同表达表明它们在发育过程中扮演着不同的角色，其中LIMK-1通过干扰促分裂原活化蛋白激酶激活的下游事件，专门抑制PC12细胞中的神经分化。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

LIMK-1 抗体 (H-12) 参考文献:

  1. 头部, 颈部和脊柱：LIMK-1 在海马中的作用  |  Sarmiere, PD. and Bamburg, JR. 2002. Neuron. 35: 3-5. PMID: 12123600
  2. LIMK和肌动蛋白细胞骨架对脊椎形态和突触功能的调节。  |  Meng, Y., et al. 2003. Rev Neurosci. 14: 233-40. PMID: 14513866
  3. LIM激酶作为精原细胞中肌动蛋白动力学的调节因子。  |  Takahashi, H., et al. 2003. Cytogenet Genome Res. 103: 290-8. PMID: 15051950
  4. 在LIM激酶抑制剂的合理设计和开发方面取得的最新进展还不足以进入临床试验。  |  Manetti, F. 2018. Eur J Med Chem. 155: 445-458. PMID: 29908439
  5. 抑制LIM激酶1和LIM激酶2可限制胶质母细胞瘤的侵袭。  |  Chen, J., et al. 2020. Cancer Res. 80: 69-78. PMID: 31641031
  6. 抑制 LIM 激酶可减少膀胱平滑肌的收缩和增殖。  |  Yu, Q., et al. 2021. Acta Pharm Sin B. 11: 1914-1930. PMID: 34386328
  7. 一种双极光和LIM激酶抑制剂可减少胶质母细胞瘤的增殖和侵袭。  |  Rybin, MJ., et al. 2022. Bioorg Med Chem Lett. 61: 128614. PMID: 35151865
  8. Par3/LIM激酶/Cofilin途径通过TAS2R14介导人类呼吸道平滑肌松弛。  |  Woo, JA., et al. 2023. Am J Respir Cell Mol Biol. 68: 417-429. PMID: 36662576
  9. 通过细胞外基质-整合素相互作用激活LIMK/Cofilin信号通路对于成熟和血管化心脏类器官的产生至关重要。  |  Noh, JM., et al. 2023. Cells. 12: PMID: 37626839
  10. LIM激酶2通过经典和非经典Wnt途径之间的相互作用激活心脏成纤维细胞并加剧梗死后左心室重塑。  |  Gong, C., et al. 2024. Pharmacol Res. 208: 107347. PMID: 39153710

订购信息

产品名称产品编号规格价格数量收藏夹

LIMK-1 抗体 (H-12)

sc-515585
200 µg/ml
RMB2377.00

LIMK-1 (H-12): m-IgG Fc BP-HRP 套装

sc-531428
200 µg Ab; 10 µg BP
RMB2662.00

LIMK-1 (H-12): m-IgGκ BP-HRP 套装

sc-525116
200 µg Ab, 40 µg BP
RMB2662.00

LIMK-1 (H-12): m-IgG1 BP-HRP 套装

sc-544549
200 µg Ab; 20 µg BP
RMB2662.00

LIMK-1 抗体 (H-12) AC

sc-515585 AC
500 µg/ml, 25% agarose
RMB3129.00

LIMK-1 抗体 (H-12) HRP

sc-515585 HRP
200 µg/ml
RMB2377.00

LIMK-1 抗体 (H-12) FITC

sc-515585 FITC
200 µg/ml
RMB2482.00

LIMK-1 抗体 (H-12) PE

sc-515585 PE
200 µg/ml
RMB2580.00

LIMK-1 抗体 (H-12) Alexa Fluor® 488

sc-515585 AF488
200 µg/ml
RMB2685.00

LIMK-1 抗体 (H-12) Alexa Fluor® 546

sc-515585 AF546
200 µg/ml
RMB2685.00

LIMK-1 抗体 (H-12) Alexa Fluor® 594

sc-515585 AF594
200 µg/ml
RMB2685.00

LIMK-1 抗体 (H-12) Alexa Fluor® 647

sc-515585 AF647
200 µg/ml
RMB2685.00

LIMK-1 抗体 (H-12) Alexa Fluor® 680

sc-515585 AF680
200 µg/ml
RMB2685.00

LIMK-1 抗体 (H-12) Alexa Fluor® 790

sc-515585 AF790
200 µg/ml
RMB2685.00

LIMK-1 (H-12) 中和勝肽

sc-515585 P
100 µg/0.5 ml
RMB511.00

What application is the blocking peptide sc-515585 P appropriate for?

Asked by: Dr Ninau Qelp
Thank you for your question. The blocking peptide is intended for use as a negative control, by pre-adsorbing the mouse monoclonal antibody against the antigen. For full protocol details, please contact our Technical Services Department or view our online protocol here: https://www.scbt.com/scbt/resources/protocols/peptide-neutralization
Answered by: Technical Support
Date published: 2017-02-27
  • y_2025, m_8, d_30, h_9CST
  • bvseo_bulk, prod_bvqa, vn_bulk_3.0.42
  • cp_1, bvpage1
  • co_hasquestionsanswers, tq_1
  • loc_zh_CN, sid_515585, prod, sort_[SortEntry(order=LAST_APPROVED_ANSWER_SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-4.0.0
  • CLOUD, getContent, 95ms
  • QUESTIONS, PRODUCT
Rated 5 out of 5 by from Good for western blotThis product works well in our western blot, shows clear bands with very good quality!
Date published: 2023-11-22
Rated 5 out of 5 by from signal band at 72 kDaThe antibody diluted 1:600 and incubate the antibody at 4°C overnight, I mainly use this antibody for detecting LIMK-1 in HeLa cells extract. Specific signal band at 72 kDa and no nonspecific bands seen.
Date published: 2017-07-29
Rated 5 out of 5 by from strong WB resultvery useful in the experiment. The analysis results of Western blot were very strong and clear.
Date published: 2017-06-03
Rated 5 out of 5 by from Strong stainingStrong staining in IF in human localized prostate cancer tissue,use the dilution 1:300.
Date published: 2017-05-28
Rated 5 out of 5 by from Great substitute to try outHi There, Back to last year I ordered Limk-1, clone 40/00-1B11-1-1 Cat. No. MAB 10750 from Millipore EMD, tried several times, it didn't give me signal, when I saw your promotion called, Ms. Jennifer Chung she responded in very helpful manner, she agreed to send me a sample of two different LIMK-1 antibodies and one blocking peptide to try out. Thanks Ms. Chung! I will let you know the outcome of the experiment with Santa Cruz antibodies: LIMK-1(H12) sc-515585 and sc-515585p. Amy Zhu UCSD
Date published: 2017-01-12
Rated 5 out of 5 by from Robust band seen in WB using embryonic stem cells.The antibody wered used in western blot and the band appeared very quickly. The band is very clear and I was very glad to see this result.
Date published: 2017-01-10
  • y_2025, m_8, d_30, h_9
  • bvseo_bulk, prod_bvrr, vn_bulk_3.0.42
  • cp_1, bvpage1
  • co_hasreviews, tv_0, tr_6
  • loc_zh_CN, sid_515585, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-4.0.0
  • CLOUD, getReviews, 19ms
  • REVIEWS, PRODUCT
LIMK-1 抗体 (H-12) is rated 5.0 out of 5 by 6.
  • y_2025, m_8, d_30, h_9
  • bvseo_bulk, prod_bvrr, vn_bulk_3.0.42
  • cp_1, bvpage1
  • co_hasreviews, tv_0, tr_6
  • loc_zh_CN, sid_515585, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-4.0.0
  • CLOUD, getAggregateRating, 107ms
  • REVIEWS, PRODUCT
021-6093-6350
访问 Santa Cruz Animal Health|访问 San Juan Ranch
Santa Cruz Biotechnology, Inc. 是生物医学研究市场产品开发的世界领导者。请拨打0086-021-60936350联系我们。
版权所有© 2007-2025 Santa Cruz Biotechnology, Inc. 保留所有权利。”Santa Cruz Biotechnology” 和Santa Cruz Biotechnology, Inc.的商标，”Santa Cruz Animal Health”, “San Juan Ranch”, “Supplement of Champions”, San Juan Ranch的商标，”UltraCruz”, “ChemCruz”, “ImmunoCruz”, “ExactaCruz”, 和 “EZ Touch”是Santa Cruz Biotechnology, Inc.的注册商标。所有商标均为其各自所有者的财产。