Date published: 2025-9-8
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LIMK-1 Antibody (C-10) - Western Blotting - Image 356640
LIMK-1 抗体 (C-10): sc-28370. C3H/10T1/2 (A), NRK (B) 和 RIN-m5F (C) 全细胞裂解液中 LIMK-1 表达的 Western 印迹分析.
LIMK-1 Antibody (C-10) - Western Blotting - Image 356640
LIMK-1 抗体 (C-10): sc-28370. C3H/10T1/2 (A), NRK (B) 和 RIN-m5F (C) 全细胞裂解液中 LIMK-1 表达的 Western 印迹分析.
LIMK-1 Antibody (C-10) - Western Blotting - Image 8852
LIMK-1 抗体 (C-10): sc-28370. HeLa (A), A-431 (B), NIH/3T3 (C) 和 PC-12 (D) 全细胞裂解液中 LIMK-1 表达的 Western 印迹分析.
LIMK-1 Antibody (C-10) - Western Blotting - Image 127031
LIMK-1 抗体 (C-10): sc-28370. 未转染: sc-117752 (A) 和转染: sc-129011 (B) 293T 全细胞裂解液中 LIMK-1 表达的 Western 印迹分析.
LIMK-1 抗体 (C-10): sc-28370. C3H/10T1/2 (A), NRK (B) 和 RIN-m5F (C) 全细胞裂解液中 LIMK-1 表达的 Western 印迹分析.

LIMK-1 抗体 (C-10): sc-28370

3.5(6)
寫評論提問

说明书
  • LIMK-1 抗体 C-10 是小鼠单克隆 IgG2b κ，LIMK-1抗体, 在11篇文献中引用，规格为200 µg/ml
  • 免疫human物种的LIMK-1的氨基酸136-219
  • LIMK-1 抗体 (C-10) 推荐用于 WB, IP, IF 和 ELISA，检测mouse, rat 和human 来源的 LIMK-1
  • 抗LIMK-1抗体（C-10）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取LIMK-1 (C-10): sc-28370的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP和 2b BP-HRP">m-IgG2b BP-HRP是LIMK-1 Antibody (C-10) 适用于 WB 应用。 的首选辅助检测试剂，这些试剂现与LIMK-1 Antibody (C-10) 打包提供（请参阅下面的订购信息）。

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描述
基因信息
蛋白序列
说明书与实验方案
研究信息

関連項目

额外LIMK抗体，包括LIMK-2

LIMK-1抗体（C-10）是一种小鼠单克隆IgG2b kappa轻链抗体，可通过蛋白质印迹（WB）、免疫沉淀（IP）、免疫荧光（IF）和酶联免疫吸附测定（ELISA）检测小鼠、大鼠和人类的LIMK-1。LIMK-1 (C-10) 抗体有非结合型和多种结合型可供选择，包括琼脂糖、辣根过氧化物酶 (HRP)、藻红蛋白 (PE)、异硫氰酸荧光素 (FITC) 和多种 Alexa Fluor® 结合物。LIMK-1是LIM激酶家族的一员，通过磷酸化并灭活Cofilin（一种切断肌动蛋白丝的蛋白质）在调节肌动蛋白细胞骨架中起着至关重要的作用。这种调节对于维持细胞结构和运动至关重要，因为LIMK-1会影响细胞迁移、形态发生和神经元分化等过程。LIMK-1调节肌动蛋白动态的能力在发育生物学和癌症转移中尤为重要，因为细胞运动的变化会导致严重的生理变化。LIMK-1和LIMK-2的表达模式表明其在发育过程中具有不同的作用，LIMK-1在某些情况下会抑制神经元的分化。了解LIMK-1的功能和调节对于阐明其对细胞过程的贡献以及以异常细胞运动为特征的疾病的潜在治疗靶点至关重要。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

LIMK-1 抗体 (C-10) 参考文献:

  1. 头部, 颈部和脊柱：LIMK-1 在海马中的作用  |  Sarmiere, PD. and Bamburg, JR. 2002. Neuron. 35: 3-5. PMID: 12123600
  2. LIMK-1基因敲除小鼠脊柱形态异常和LTP增强  |  Meng, Y., et al. 2002. Neuron. 35: 121-33. PMID: 12123613
  3. miR-143-3p靶向LIM域激酶1抑制三阴性乳腺癌细胞的进展。  |  Li, D., et al. 2017. Am J Transl Res. 9: 2276-2285. PMID: 28559978
  4. MiR-519d-3p通过靶向LIM域激酶1抑制乳腺癌细胞的生长和运动。  |  Li, D., et al. 2018. Mol Cell Biochem. 444: 169-178. PMID: 29188531
  5. 6-Hydroxythiobinupharidine 通过降低 LIM Domain Kinase 1 的表达抑制 LM8 骨肉瘤细胞的迁移。  |  Yoshizawa, M., et al. 2019. Anticancer Res. 39: 6507-6513. PMID: 31810915
  6. HUNK通过直接磷酸化GEF-H1并激活RhoA/LIMK-1/CFL-1抑制CRC的上皮-间质转化。  |  Han, X., et al. 2023. Cell Death Dis. 14: 327. PMID: 37193711
  7. LIMK-1 和 LIMK-2，含 LIM 基调蛋白激酶家族的两个成员。  |  Nunoue, K., et al. 1995. Oncogene. 11: 701-10. PMID: 7651734
  8. 含有两个 N--terminal LIM motifs 的新型丝氨酸/苏氨酸激酶家族的鉴定和特征描述。  |  Okano, I., et al. 1995. J Biol Chem. 270: 31321-30. PMID: 8537403
  9. 比较发育中大鼠体内含有 LIM 矩阵的两种新型丝氨酸/苏氨酸激酶基因（LIMK-1 和 LIMK-2）的组织分布。  |  Mori, T., et al. 1997. Brain Res Mol Brain Res. 45: 247-54. PMID: 9149099
  10. LIM-kinase 1 的 LIM 结构域抑制活化的 Ras 诱导的 PC12 细胞神经元分化。  |  Higuchi, O., et al. 1997. Oncogene. 14: 1819-25. PMID: 9150388
  11. LIM 激酶 1 磷酸化的 Cofilin 及其在 Rac 介导的肌动蛋白重组中的作用  |  Yang, N., et al. 1998. Nature. 393: 809-12. PMID: 9655398
  12. 编码截短的 LIM 激酶 2 的新型转录本在进行减数分裂的雄性生殖细胞中特异性表达。  |  Takahashi, H., et al. 1998. Biochem Biophys Res Commun. 249: 138-45. PMID: 9705845

订购信息

产品名称产品编号规格价格数量收藏夹

LIMK-1 抗体 (C-10)

sc-28370
200 µg/ml
RMB2377.00

LIMK-1 (C-10): m-IgG Fc BP-HRP 套装

sc-525529
200 µg Ab; 10 µg BP
RMB2662.00

LIMK-1 (C-10): m-IgG2b BP-HRP 套装

sc-549572
200 µg Ab; 10 µg BP
RMB2662.00

LIMK-1 抗体 (C-10) AC

sc-28370 AC
500 µg/ml, 25% agarose
RMB3129.00

LIMK-1 抗体 (C-10) HRP

sc-28370 HRP
200 µg/ml
RMB2377.00

LIMK-1 抗体 (C-10) FITC

sc-28370 FITC
200 µg/ml
RMB2482.00

LIMK-1 抗体 (C-10) PE

sc-28370 PE
200 µg/ml
RMB2580.00

LIMK-1 抗体 (C-10) Alexa Fluor® 488

sc-28370 AF488
200 µg/ml
RMB2685.00

LIMK-1 抗体 (C-10) Alexa Fluor® 546

sc-28370 AF546
200 µg/ml
RMB2685.00

LIMK-1 抗体 (C-10) Alexa Fluor® 594

sc-28370 AF594
200 µg/ml
RMB2685.00

LIMK-1 抗体 (C-10) Alexa Fluor® 647

sc-28370 AF647
200 µg/ml
RMB2685.00

LIMK-1 抗体 (C-10) Alexa Fluor® 680

sc-28370 AF680
200 µg/ml
RMB2685.00

LIMK-1 抗体 (C-10) Alexa Fluor® 790

sc-28370 AF790
200 µg/ml
RMB2685.00

Is it possible to receive a free sample of anti-LIMK1 sc-28370 ?

Asked by: ASR1209
Thank you for your question. If you have an interest in testing a free 10 µg sample of LIMK-1 (C-10): sc-28370 please contact our Technical Service by phone or email or by Live Chat.
Answered by: SCBT Heidelberg Support
Date published: 2018-11-27

Are there any issues with using sc-28370: LIMK-1 (C-10) mouse monoclonal primary antibody on mouse samples?

Asked by: Dr Ninau Qelp
Thank you for your inquiry. The use of mouse monoclonal antibodies with mouse samples is very common and typically poses no problem. To eliminate any potential cross-reactivity of an anti-mouse secondary detection reagent,we recommend using a directly conjugated primary antibody. Please contact our special orders department for pricing and availability of sc-28370 conjugates.
Answered by: Technical Support
Date published: 2017-03-07

Hi, I wish to get a free sample to try a preliminary assay and reconfirm the antibody is working well. Could you please let me know whether I can get it? Thanks.

Asked by: gowsy
Thank you for your question. We are currently offering a free 10 µg sample of one of our highly recommended mouse monoclonals, LIMK-1 Antibody (H-12): sc-515585 (https://www.scbt.com/scbt/product/limk-1-antibody-h-12). We are not offering a sample size of this specific product, LIMK-1 Antibody (C-10): sc-28370. Santa Cruz Biotechnology does warranty our antibodies for one year from the date of purchase. Please contact our Technical Service Department to inquire about a free sample. Please call us at (800) 457-3801 option 2, or email [email protected], or use our live chat.
Answered by: Technical Support
Date published: 2017-02-03
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Rated 1 out of 5 by from Poor signal and strong backgroundIt shows very poor signal in western blot using whole protein extracts from MCF7 and MB-231 cells. Lots of unspecific bands
Date published: 2019-09-18
Rated 5 out of 5 by from Reliable Antibody, Great for WBThis antibody is well-cited for a variety of applications including WB, IP, IF, and even ELISA. Strong, clear signal with little to no background. Works well in a variety of species including mouse, rat, and human samples.
Date published: 2017-02-07
Rated 3 out of 5 by from Worked with transfected lysate by WB withWorked with transfected lysate by WB with some background -SCBT QC
Date published: 2015-07-20
Rated 4 out of 5 by from Positive result in WB using HeLaPositive result in WB using HeLa, A-431, NIH/3T3 and PC-12 whole cell lysates. -SCBT QC
Date published: 2015-03-29
Rated 4 out of 5 by from Publishable data in IP using human MDAPublishable data in IP using human MDA-MA-468 -SCBT Publication Review
Date published: 2015-01-19
Rated 4 out of 5 by from Publishable data in WB using human HCC(hepatocellularPublishable data in WB using human HCC(hepatocellular carcinoma) specimens and adjacent non-cancerous specimens -SCBT Publication Review
Date published: 2014-01-20
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LIMK-1 抗体 (C-10) is rated 3.5 out of 5 by 6.
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