Date published: 2025-9-8
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LIFR Antibody (A-10) - Western Blotting - Image 404921
LIFR 抗体 (A-10): sc-515337. HeLa (A) 和 Sol8 (B) 全细胞裂解液中 LIFR 表达的荧光西部印迹分析. 用 UltraCruz® 阻断试剂阻断: sc-516214. 所用检测试剂: m-IgG1 BP-CFL 488: sc-533661.
LIFR 抗体 (A-10): sc-515337. HeLa (A) 和 Sol8 (B) 全细胞裂解液中 LIFR 表达的荧光西部印迹分析. 用 UltraCruz® 阻断试剂阻断: sc-516214. 所用检测试剂: m-IgG1 BP-CFL 488: sc-533661.

LIFR 抗体 (A-10): sc-515337

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寫評論提問

说明书
  • LIFR 抗体 A-10 是小鼠单克隆 IgG1 κ，LIFR抗体, 在20篇文献中引用，规格为200 µg/ml
  • 免疫human物种的LIFR的氨基酸878-1097
  • LIFR 抗体 (A-10) 推荐用于 WB, IP, IF 和 ELISA，检测mouse, rat 和human 来源的 LIFR
  • 抗LIFR抗体（A-10）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取LIFR (A-10): sc-515337的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP和 1 BP-HRP">m-IgG1 BP-HRP是LIFR Antibody (A-10) for WB applications. 的首选辅助检测试剂，这些试剂现与LIFR Antibody (A-10) 打包提供（请参阅下面的订购信息）。

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描述
基因信息
说明书与实验方案
研究信息

関連項目

LIFR抗体（A-10）是一种小鼠单克隆IgG1 kappa轻链抗体，可通过蛋白质印迹（WB）、免疫沉淀（IP）、免疫荧光（IF）和酶联免疫吸附测定（ELISA）检测小鼠、大鼠和人类来源的LIFR蛋白。LIFR抗体（A-10）有非结合型和多种结合型可供选择，包括琼脂糖、辣根过氧化物酶（HRP）、藻红蛋白（PE）、异硫氰酸荧光素（FITC）和多种Alexa Fluor®结合物。LIFR蛋白在调节白血病抑制因子（LIF）的作用方面发挥着关键作用，而LIF对于胚胎干细胞的维持和分化以及免疫反应的调节等各种生物过程至关重要。 LIFR主要位于细胞表面，与gp130形成信号复合物，后者是多种细胞因子的共同受体成分。 这种复合物对于传递影响细胞存活、增殖和分化的信号至关重要。在炎症和组织修复过程中，LIF和LIFR之间的相互作用尤为重要，因为LIFR有助于调节免疫反应，并在压力条件下促进特定细胞类型的存活。了解LIFR的功能和信号通路有助于了解其在癌症和自身免疫性疾病等各种疾病中的作用，使抗LIFR抗体（A-10）成为研究人员研究这些关键生物学过程的宝贵工具。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

LIFR 抗体 (A-10) 参考文献:

  1. IL-6 信号转导子 gp130 的分子克隆和表达。  |  Hibi, M., et al. 1990. Cell. 63: 1149-57. PMID: 2261637
  2. 白细胞介素-6 会触发其受体与可能的信号转导物 gp130 的结合。  |  Taga, T., et al. 1989. Cell. 58: 573-81. PMID: 2788034
  3. 人白细胞介素-6（BSF-2/IFN beta 2）受体的克隆与表达。  |  Yamasaki, K., et al. 1988. Science. 241: 825-8. PMID: 3136546
  4. 白血病抑制因子通过 LIFR-Hippo-YAP 通路促进胃癌细胞增殖, 迁移和侵袭。  |  Bian, SB., et al. 2021. Ann N Y Acad Sci. 1484: 74-89. PMID: 32827446
  5. 由细胞外赖氨酸乙酰化介导的白血病抑制因子受体同源二聚体化通过 PDPK1/AKT/GCN5 轴促进前列腺癌的进展。  |  Ding, Y., et al. 2022. Clin Transl Med. 12: e676. PMID: 35172032
  6. 产前雄激素诱导多囊卵巢综合征模型大鼠胚胎着床部位白血病抑制因子（LIF）、LIFR、gp130和IL11的表达改变。  |  Javidan, M., et al. 2022. Biochem Biophys Res Commun. 605: 24-30. PMID: 35306361
  7. 小鼠 IL-11 受体α-链的克隆；高亲和力结合和信号转导需要 gp130。  |  Hilton, DJ., et al. 1994. EMBO J. 13: 4765-75. PMID: 7957045
  8. 可溶性人类 IL-6 受体。蛋白水解裂解位点的突变特征。  |  Müllberg, J., et al. 1994. J Immunol. 152: 4958-68. PMID: 8176214
  9. 细胞因子信号转导  |  Kishimoto, T., et al. 1994. Cell. 76: 253-62. PMID: 8293462
  10. LIFR beta 和 gp130 作为三方 CNTF 受体的异源二聚体信号转导子。  |  Davis, S., et al. 1993. Science. 260: 1805-8. PMID: 8390097
  11. 白细胞介素 6 信号转导子 gp130 的关键细胞质区域在细胞因子受体家族中是保守的。  |  Murakami, M., et al. 1991. Proc Natl Acad Sci U S A. 88: 11349-53. PMID: 1662392
  12. IL-6 诱导的 gp130 同源二聚化及相关的酪氨酸激酶激活。  |  Murakami, M., et al. 1993. Science. 260: 1808-10. PMID: 8511589

订购信息

产品名称产品编号规格价格数量收藏夹

LIFR 抗体 (A-10)

sc-515337
200 µg/ml
RMB2377.00

LIFR (A-10): m-IgG Fc BP-HRP 套装

sc-528025
200 µg Ab; 10 µg BP
RMB2662.00

LIFR (A-10): m-IgG1 BP-HRP 套装

sc-533398
200 µg Ab; 20 µg BP
RMB2662.00

LIFR 抗体 (A-10) AC

sc-515337 AC
500 µg/ml, 25% agarose
RMB3129.00

LIFR 抗体 (A-10) HRP

sc-515337 HRP
200 µg/ml
RMB2377.00

LIFR 抗体 (A-10) FITC

sc-515337 FITC
200 µg/ml
RMB2482.00

LIFR 抗体 (A-10) PE

sc-515337 PE
200 µg/ml
RMB2580.00

LIFR 抗体 (A-10) Alexa Fluor® 488

sc-515337 AF488
200 µg/ml
RMB2685.00

LIFR 抗体 (A-10) Alexa Fluor® 546

sc-515337 AF546
200 µg/ml
RMB2685.00

LIFR 抗体 (A-10) Alexa Fluor® 594

sc-515337 AF594
200 µg/ml
RMB2685.00

LIFR 抗体 (A-10) Alexa Fluor® 647

sc-515337 AF647
200 µg/ml
RMB2685.00

LIFR 抗体 (A-10) Alexa Fluor® 680

sc-515337 AF680
200 µg/ml
RMB2685.00

LIFR 抗体 (A-10) Alexa Fluor® 790

sc-515337 AF790
200 µg/ml
RMB2685.00

What was the dilution used to detect LIFR in HeLa cells?

Asked by: Valeria
Thank you for your question. Depending on your samples and experimental setup we recommend a starting dilution of 1:100 for 40–60 µg whole cell lysate, 10–20 µg nuclear extract, 5-10 µg transfected lysate, or 10–20 ng purified protein per lane. Otherwise, the antibody's dilution range is between 1:100 and 1:1000.
Answered by: Tech Support Europe
Date published: 2023-03-21

I'd like to know which part of amino acid sequence (AA878-1097) this antibody specifically binds to.

Asked by: RhoA
Thank you for your question. LIFR (A-10): sc-515337 is a mouse monoclonal antibody raised against a recombinant protein corresponding to amino acids 878-1097 of LIFR of human origin. The exact epitope (amino acid sequence) recognized by this antibody has not been determined.
Answered by: Technical Support
Date published: 2017-05-02

I need to use an antibody to detect the protein in samples of avian origin. Do you know if sc-515337: LIFR (A-10) mouse monoclonal antibody will work?

Asked by: TinTin
Please contact our Technical Service Department and we would be happy to perform sequence analysis and review our data to answer any questions you may have about additional species reactivity.
Answered by: Technical Support
Date published: 2017-03-06

We want an antibody to block mouse LIFR on cells. Which antibody would we choose? Thanks!

Asked by: Frances
Thank you for your question. Santa Cruz Biotechnology does not test any of our LIFR antibodies for biological activity, or use in blocking or neutralizing research applications. And we are not aware of any publications citing any of our antibodies for this type of application.
Answered by: Technical Support
Date published: 2017-01-31
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Rated 5 out of 5 by from Good for Western BlotAntibody works for Western blot with OP9 Bone Marrow Stromal cell lysate in published research. -SCBT QC
Date published: 2023-09-14
Rated 4 out of 5 by from LIFR in HEK-293 cellsI asked for a sample of the LIFR antibody (A-10): SC-515337 for Western Blot, I tried it with HEK-293 cells (dilution 1:500), and I obtained the protein band in another molecular weight (45 kDa)
Date published: 2023-03-03
Rated 5 out of 5 by from Highly recommendedI have got a strong band in my western blotting experiment.
Date published: 2017-08-30
Rated 5 out of 5 by from 挺好的用来做WB 的，条带是跑出来了，但趋势不对，可能是样品本身蛋白的问题，得再做一些试试看吧，希望结果不错吧
Date published: 2017-08-01
Rated 5 out of 5 by from 很好申请的试用装很不错，还可用于兔来源的组织。感觉效果跑出来还可以，就是目标条带不在预测的位置，不知道是怎么回事。
Date published: 2017-02-17
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LIFR 抗体 (A-10) is rated 4.8 out of 5 by 5.
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