Date published: 2025-9-7
访问圣克鲁斯动物健康

021-6093-6350

SCBT Portrait Logo
Seach Input
联系我们
登陆账户
快速订购
购物车 (0)
Legumain Antibody (B-8) - Western Blotting - Image 356926
Legumain 抗体 (B-8): sc-133234. HT-29 (A), HeLa (B), RAW 264. 7 (C), Neuro-2A (D), RPE-J (E) 和 RIN-m5F (F) 全细胞裂解液中 Legumain 表达的 Western 印迹分析.
Legumain 抗体 (B-8): sc-133234. HT-29 (A), HeLa (B), RAW 264. 7 (C), Neuro-2A (D), RPE-J (E) 和 RIN-m5F (F) 全细胞裂解液中 Legumain 表达的 Western 印迹分析.

Legumain 抗体 (B-8): sc-133234

4.9(7)
寫評論提問

说明书
  • Legumain 抗体 B-8 是小鼠单克隆 IgG1 κ，Legumain抗体, 在9篇文献中引用，规格为200 µg/ml
  • 免疫human来源Legumain近N-terminus的21-320氨基酸
  • Legumain 抗体 (B-8) 推荐用于 WB, IP, IF, IHC(P) 和 ELISA，检测mouse, rat 和human 来源的 precursor and mature Legumain
  • 抗Legumain抗体（B-8）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取Legumain (B-8): sc-133234的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP, m-IgG1 BP-HRP 和 m-IgGκ BP-HRP 是 Legumain 抗体 (B-8) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与Legumain 抗体 (B-8) 组合成套装提供。 (请参阅下面的订购信息)。

快捷链接

相关产品
描述
基因信息
蛋白序列
说明书与实验方案
研究信息

関連項目

Legumain抗体（B-8）是一种IgG1 κ小鼠单克隆Legumain抗体（也称为Legumain抗体），可通过WB、IP、IF、IHC(P)和ELISA等方法检测小鼠、大鼠和人类来源的Legumain蛋白。Legumain抗体（B-8）既有非偶联抗Legumain抗体形式，也有多种偶联形式的抗Legumain抗体，包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®偶联物。Legumain，也被称为LGMN、AEP（天冬氨酰内肽酶）或PRSC1，是一种由433个氨基酸组成的蛋白质，它定位于溶酶体，属于肽酶C13家族。Legumain在胎盘、心脏和肾脏中的表达量特别高，且无处不在，它作为一种半胱氨酸蛋白酶，专门催化天冬氨酰和天冬氨酸键的水解。此外，Legumain被认为参与了溶酶体/内体系统中MHC II类抗原呈递的细菌蛋白的处理过程。Legumain以树突状细胞中产生的前体和完全成熟的活性酶两种形式存在。Legumain的过度表达可能与实体瘤的形成有关，这表明Legumain在致癌过程中发挥作用。由于选择性剪接事件，Legumain存在多种异构体。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

Legumain 抗体 (B-8) 参考文献:

  1. 天冬酰胺酰内肽酶在体外和体内的多步自激活。  |  Li, DN., et al. 2003. J Biol Chem. 278: 38980-90. PMID: 12860980
  2. 控制人类 B 淋巴细胞溶酶体中髓鞘碱性蛋白加工的是胰蛋白酶 G，而不是天冬酰胺特异性内切蛋白酶。  |  Burster, T., et al. 2004. J Immunol. 172: 5495-503. PMID: 15100291
  3. Legumain 表达与结直肠癌临床病理学和生物学变量的关系  |  Murthy, RV., et al. 2005. Clin Cancer Res. 11: 2293-9. PMID: 15788679
  4. PIKE-L 通过抑制天冬酰胺内肽酶对 SET 的蛋白水解作用起到神经保护作用。  |  Liu, Z., et al. 2008. Mol Cell. 29: 665-78. PMID: 18374643
  5. 半胱氨酸蛋白酶和豆蛋白酶介导的蛋白质分解--最新进展和细胞生物学挑战。  |  Brix, K., et al. 2015. Protoplasma. 252: 755-74. PMID: 25398648
  6. 癌症中豆豆蛋白酶过表达的临床病理学意义：系统综述和荟萃分析。  |  Zhen, Y., et al. 2015. Sci Rep. 5: 16599. PMID: 26607955
  7. 以 Legumain 为靶点作为癌症的新型治疗策略  |  Mai, CW., et al. 2017. Curr Drug Targets. 18: 1259-1268. PMID: 27993111
  8. 天冬酰胺酰内肽酶 Legumain 介导 M2 巨噬细胞减轻阻塞性肾病肾间质纤维化的作用。  |  Wang, D., et al. 2018. Kidney Int. 94: 91-101. PMID: 29656902
  9. Legumain 介导的用于肿瘤靶向放射治疗的 131I 标记制剂的自组装。  |  Wang, Q., et al. 2022. J Mater Chem B. 10: 2251-2259. PMID: 35297450
  10. 活性豆豆蛋白酶通过调节上皮-间质转化促进神经母细胞瘤的侵袭和迁移。  |  Zhang, M., et al. 2022. Open Life Sci. 17: 676-685. PMID: 35800070
  11. 编码假定半胱氨酸蛋白酶（PRSC1）的人类 cDNA 的分子克隆及其在 14q32.1 的染色体定位。  |  Tanaka, T., et al. 1996. Cytogenet Cell Genet. 74: 120-3. PMID: 8893817
  12. 哺乳动物豆豆蛋白酶（一种天冬酰胺酰内肽酶）的克隆, 分离和表征。  |  Chen, JM., et al. 1997. J Biol Chem. 272: 8090-8. PMID: 9065484

订购信息

产品名称产品编号规格价格数量收藏夹

Legumain 抗体 (B-8)

sc-133234
200 µg/ml
RMB2377.00

Legumain (B-8): m-IgG Fc BP-HRP 套装

sc-528779
200 µg Ab; 10 µg BP
RMB2662.00

Legumain (B-8): m-IgGκ BP-HRP 套装

sc-521286
200 µg Ab, 40 µg BP
RMB2662.00

Legumain (B-8): m-IgG1 BP-HRP 套装

sc-543123
200 µg Ab; 20 µg BP
RMB2662.00

Legumain 抗体 (B-8) AC

sc-133234 AC
500 µg/ml, 25% agarose
RMB3129.00

Legumain 抗体 (B-8) HRP

sc-133234 HRP
200 µg/ml
RMB2377.00

Legumain 抗体 (B-8) FITC

sc-133234 FITC
200 µg/ml
RMB2482.00

Legumain 抗体 (B-8) PE

sc-133234 PE
200 µg/ml
RMB2580.00

Legumain 抗体 (B-8) Alexa Fluor® 488

sc-133234 AF488
200 µg/ml
RMB2685.00

Legumain 抗体 (B-8) Alexa Fluor® 546

sc-133234 AF546
200 µg/ml
RMB2685.00

Legumain 抗体 (B-8) Alexa Fluor® 594

sc-133234 AF594
200 µg/ml
RMB2685.00

Legumain 抗体 (B-8) Alexa Fluor® 647

sc-133234 AF647
200 µg/ml
RMB2685.00

Legumain 抗体 (B-8) Alexa Fluor® 680

sc-133234 AF680
200 µg/ml
RMB2685.00

Legumain 抗体 (B-8) Alexa Fluor® 790

sc-133234 AF790
200 µg/ml
RMB2685.00

Thank you for your support. I purchased Legumain antibodies at your company. I stained with immunofluorescent histochemistry, but I can't stain it well. I use 4% paraformaldehyde for tissue fixation, but is it better to fix it with methanol?

Asked by: shigeta
Thank you for your question. Yes, you can try different fixation or antigen-Retrieval performed.Please contact our Technical Service Department of Japan for the consultation optimization suggestion and more information.
Answered by: Jiajun Z
Date published: 2024-12-09

I am using Legumain (B-8): sc-133234 monoclonal antibody to stain paraffin embedded tissue. Do you recommend antigen retrieval when using this antibody?

Asked by: cjMara
We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-02-25
  • y_2025, m_9, d_7, h_4CST
  • bvseo_bulk, prod_bvqa, vn_bulk_3.0.42
  • cp_1, bvpage1
  • co_hasquestionsanswers, tq_2
  • loc_zh_CN, sid_133234, prod, sort_[SortEntry(order=LAST_APPROVED_ANSWER_SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-4.0.0
  • CLOUD, getContent, 120ms
  • QUESTIONS, PRODUCT
Rated 5 out of 5 by from hippocampusit just works well at 1:1000 in rat hippocampus lysates.
Date published: 2021-03-30
Rated 4 out of 5 by from No Positivity in S. aurata and D. labrax tissuesAt the tested dilution (1:50, 1:200) and with the protocol in use in our laboratories, it was not possible to detect any positivity in Sparus aurata and Dicentrarchus labrax tissues
Date published: 2019-07-03
Rated 5 out of 5 by from gave strong bandLegumain antibody can diluted 1:800 for Hela cell detection by WB.We used Hela cell as positive control,it's shows positive band at 50kda.I suggest use 5% BSA in TBS (without Tween) for blocking at RT for 1hour.This blocking condition is optimized ,no background.
Date published: 2017-09-09
Rated 5 out of 5 by from worked succesfullyThis antibody worked succesfully at diluted 1:500 for Legumain protein detection. Blocking buffer was used 5% non-fat milk for 1 h ,stong band obtained at 55kda.
Date published: 2017-09-02
Rated 5 out of 5 by from clear bandThis antibody was used to WB assay in human placental cells. The dilution used was 1:1000. The main band was clear.
Date published: 2017-06-03
Rated 5 out of 5 by from Great immunoperoxidase cytoplasmic stainingGreat immunoperoxidase cytoplasmic staining in formalin fixed, paraffin-embedded human placenta tissue. -SCBT QC
Date published: 2015-01-13
  • y_2025, m_9, d_7, h_4
  • bvseo_bulk, prod_bvrr, vn_bulk_3.0.42
  • cp_1, bvpage1
  • co_hasreviews, tv_1, tr_6
  • loc_zh_CN, sid_133234, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-4.0.0
  • CLOUD, getReviews, 10ms
  • REVIEWS, PRODUCT
Legumain 抗体 (B-8) is rated 4.9 out of 5 by 7.
  • y_2025, m_9, d_7, h_4
  • bvseo_bulk, prod_bvrr, vn_bulk_3.0.42
  • cp_1, bvpage1
  • co_hasreviews, tv_1, tr_6
  • loc_zh_CN, sid_133234, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-4.0.0
  • CLOUD, getAggregateRating, 106ms
  • REVIEWS, PRODUCT
021-6093-6350
访问 Santa Cruz Animal Health|访问 San Juan Ranch
Santa Cruz Biotechnology, Inc. 是生物医学研究市场产品开发的世界领导者。请拨打0086-021-60936350联系我们。
版权所有© 2007-2025 Santa Cruz Biotechnology, Inc. 保留所有权利。”Santa Cruz Biotechnology” 和Santa Cruz Biotechnology, Inc.的商标，”Santa Cruz Animal Health”, “San Juan Ranch”, “Supplement of Champions”, San Juan Ranch的商标，”UltraCruz”, “ChemCruz”, “ImmunoCruz”, “ExactaCruz”, 和 “EZ Touch”是Santa Cruz Biotechnology, Inc.的注册商标。所有商标均为其各自所有者的财产。