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Laminin α-2 Antibody (B-4) - Western Blotting - Image 380809 — 层粘蛋白 α-2 抗体 (B-4): sc-55605. Caki-1 (A), WI-38 (B), NIH/3T3 (C) 和 PC-12 (D) 全细胞裂解液及大鼠胚胎组织提取物 (E) 中层粘蛋白 α-2 表达的 Western 印迹分析.

层粘蛋白 α-2 抗体 (B-4): sc-55605. Caki-1 (A), WI-38 (B), NIH/3T3 (C) 和 PC-12 (D) 全细胞裂解液及大鼠胚胎组织提取物 (E) 中层粘蛋白 α-2 表达的 Western 印迹分析.

Laminin α-2 抗体 (B-4): sc-55605

Name: Laminin &alpha;-2抗体 (B-4)
Brand: SCBIO
SKU: sc-55605
Price: 2377.0 CNY
Availability: InStock

5.0(2)

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说明书

Laminin alpha-2 抗体 B-4 是小鼠单克隆 IgG₁ κ，Laminin α-2抗体, 在13篇文献中引用，规格为200 µg/ml
免疫human来源Laminin α-2近C-terminus的2811-2940氨基酸
Laminin alpha-2 抗体 (B-4) 推荐用于 WB, IP, IF 和 ELISA，检测mouse, rat 和human 来源的 Laminin α-2
抗Laminin alpha-2抗体（B-4）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
还可偶联Alexa Fluor^® 488, Alexa Fluor^® 546, Alexa Fluor^® 594 和 Alexa Fluor^® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
还可偶联Alexa Fluor^® 680 和 Alexa Fluor^® 790，用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR^®/Odyssey^®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
关于如何获取Laminin α-2 (B-4): sc-55605的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
m-IgG Fc BP-HRP、 1 BP-HRP">m-IgG₁ BP-HRP和m-IgGκ BP-HRP是Laminin α-2 Antibody (B-4) 适用于 WB 应用。的首选辅助检测试剂。这些试剂现与Laminin α-2 Antibody (B-4) 打包提供（请参阅下面的订购信息）。

查看产品引用文献(13)

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Laminin α-2 抗体 (B-4) 参考文献:

层粘连蛋白{α}1链可纠正因缺乏层粘连蛋白{α}2链而导致的男性不育症。 | Häger, M., et al. 2005. Am J Pathol. 167: 823-33. PMID: 16127160
层粘连蛋白α2链可促进胶质母细胞瘤干细胞生长。 | Lathia, JD., et al. 2012. Ann Neurol. 72: 766-78. PMID: 23280793
分泌层粘连蛋白α2的成纤维细胞可增强骨骼肌细胞片的治疗效果。 | Uchinaka, A., et al. 2017. Eur J Cardiothorac Surg. 51: 457-464. PMID: 27663298
胶原6和层粘连蛋白α2先天性肌肉萎缩症患者的电阻抗肌电图：一项为期两年的横断面分析。 | Nichols, C., et al. 2018. Muscle Nerve. 57: 54-60. PMID: 28224647
提高层粘连蛋白-α2相关先天性肌营养不良症DyW小鼠模型中表型评估的可重复性。 | Willmann, R., et al. 2017. J Neuromuscul Dis. 4: 115-126. PMID: 28550268
LAMA2基因突变更新：更全面地了解层粘连蛋白-α2变异体及其相关表型。 | Oliveira, J., et al. 2018. Hum Mutat. 39: 1314-1337. PMID: 30055037
PDGFRβ阳性细胞介导的纤维粘连蛋白和层粘连蛋白α2的卒中后重塑有助于组织修复和功能恢复。 | Shibahara, T., et al. 2023. J Cereb Blood Flow Metab. 43: 518-530. PMID: 36514952
骨骼肌中层粘连蛋白-α2链的缺乏会导致多种细胞机制失调。 | Martins, SG., et al. 2024. Life Sci Alliance. 7: PMID: 39379105
骨骼肌中保存的梅罗辛M链（或层粘连蛋白-α2）表达将沃克-沃堡综合征与福山型肌营养不良症和梅罗辛缺乏型先天性肌营养不良症区分开来。 | Voit, T., et al. 1995. Neuropediatrics. 26: 148-55. PMID: 7477753
先天性肌营养不良症伴层粘连蛋白α2链缺乏：鉴定新的中间表型以及临床发现与肌肉免疫组织化学的相关性。 | Herrmann, R., et al. 1996. Eur J Pediatr. 155: 968-76. PMID: 8911899

订购信息

产品名称	产品编号	规格	价格	数量	收藏夹
Laminin α-2 抗体 (B-4)	sc-55605	200 µg/ml	RMB2377.00
Laminin α-2 (B-4): m-IgG Fc BP-HRP 套装	sc-528612	200 µg Ab; 10 µg BP	RMB2662.00
Laminin α-2 (B-4): m-IgGκ BP-HRP 套装	sc-521032	200 µg Ab, 40 µg BP	RMB2662.00
Laminin α-2 (B-4): m-IgG₁ BP-HRP 套装	sc-543027	200 µg Ab; 20 µg BP	RMB2662.00
Laminin α-2 抗体 (B-4) AC	sc-55605 AC	500 µg/ml, 25% agarose	RMB3129.00
Laminin α-2 抗体 (B-4) HRP	sc-55605 HRP	200 µg/ml	RMB2377.00
Laminin α-2 抗体 (B-4) FITC	sc-55605 FITC	200 µg/ml	RMB2482.00
Laminin α-2 抗体 (B-4) PE	sc-55605 PE	200 µg/ml	RMB2580.00
Laminin α-2 抗体 (B-4) Alexa Fluor^® 488	sc-55605 AF488	200 µg/ml	RMB2685.00
Laminin α-2 抗体 (B-4) Alexa Fluor^® 546	sc-55605 AF546	200 µg/ml	RMB2685.00
Laminin α-2 抗体 (B-4) Alexa Fluor^® 594	sc-55605 AF594	200 µg/ml	RMB2685.00
Laminin α-2 抗体 (B-4) Alexa Fluor^® 647	sc-55605 AF647	200 µg/ml	RMB2685.00
Laminin α-2 抗体 (B-4) Alexa Fluor^® 680	sc-55605 AF680	200 µg/ml	RMB2685.00
Laminin α-2 抗体 (B-4) Alexa Fluor^® 790	sc-55605 AF790	200 µg/ml	RMB2685.00

Hello, My name is zeynab. I have this antibody and I want to do immunostaining for NET using brain sample. what concentration of primary and secondary antibody you are suggesting. best zeynab

Asked by: tabrizi

Thank you for your question. Primary antibody we recommend (1:50-1:500) and secondary (1:25-1:100) .

Answered by: BlakeJ

Date published: 2024-11-22

Hello  I would like to know if it’s recommended to use BSA or non fat milk as a diluent for the antibody and to block the membrane ?  I’m using antibody for laminin alpha 2 subunit (sc-55605) Thank you in advance 

Asked by: S Tlep

For a diluent, we recommend using PBS. You can use either BSA, non-fat milk, or our UltraCruz Blocking Reagent sc-516214 as a blocking reagent.

Answered by: Dallas Tech Service

Date published: 2021-06-18

I am using the LiCor/Odyssey system. Which secondary antibody do you recommend using with Laminin α-2, sc-55605?

Asked by: TinTin

We recommend using one of our our exclusive Mouse IgG Binding Proteins (m-IgGκ) conjugated to CFL 680 or CFL 790. They are suitable for use with Near-Infrared (NIR) detection systems, such as LI-COR/Odyssey and other comparable systems. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety

Answered by: Tech Service

Date published: 2017-01-27

Hello, I have a question about Laminin α-2 Antibody (B-4): sc-55605; I want to know if this antibody can used in 2 western blot and Immunohistochemistry techniques?

Asked by: Mahdieh

Thank you for your question. Laminin α-2 Antibody (B-4) is a mouse monoclonal that is recommended for detection of Laminin α-2 of mouse, rat and human origin by WB (Western blot) and IF (immunofluorescence or immunohistochemistry on fresh or frozen tissues or cells fixed in methanol or acetone). This antibody has not yet been tested on formalin-fixed paraffin-embedded tissue sections.

Answered by: Technical Support

Date published: 2016-12-14

Rated 5 out of 5 by Germaine from Clear strong bands in WBVery clean and distinct strong bands in Caki-1 and HeLa whole cell lysates and rat lung tissue extract.

Date published: 2016-12-23

Rated 5 out of 5 by LF from Great Western blot data of Laminin αGreat Western blot data of Laminin α-2 expression in mouse embryo tissue extract. -SCBT QC

Date published: 2013-06-30

Laminin α-2 抗体 (B-4) is rated 5.0 out of 5 by 2.

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种属	基因名称	基因ID	染色体位置	亚型（mRNA）登录号	蛋白质登录号	OMIM™ 号码
Human	LAMA2	Entrez Gene ID: 3908	6q22.33	XM_005266981, XM_005266982, XM_011535820, XM_017010851, XM_017010852, XM_017010853, NM_000426, NM_001079823	UniProt ID: P24043	607855
Mouse	Lama2	Entrez Gene ID: 16773	10 A4	XM_006512566, XM_011243134, XM_011243135, XM_017313815, NM_008481	UniProt ID: Q60675	N/A

Laminin α-2 抗体 (B-4): sc-55605

Laminin α-2 抗体 (B-4) 参考文献:

Laminin α-2 抗体 (B-4) 规格

替代产品名称: Laminin α-2

产品克隆名称: B-4

分类: 一抗

Clonality: 单克隆抗体

抗体同型: IgG₁ kappa light chain

氨基酸: 2811-2940

抗原表位靶标: Laminin α-2

抗原表位种属: human

产品反应性/检测: Laminin α-2

种属反应性/检测: mouse, rat, human

其它哺乳动物反应性: N/A

其它非哺乳动物反应性: N/A

应用: WB, IP, IF, ELISA

瓶的信息: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

研究引文: 13篇各种科学出版物的引文

Laminin α-2 抗体 (B-4)可选的偶联物/形式: AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin

優選的 Laminin α-2 抗體是 Laminin α-2 抗體 (4H8-2): sc-59854.