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Lamin B1 抗体 (C-12): sc-365214

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说明书
  • Lamin B1抗体(C-12)是小鼠单克隆IgG2b κ, 在33篇文献中引用,规格为200 µg/ml
  • 特异性抗原位于human物种的Lamin B11的C-terminus的氨基酸559-584之间
  • 抗-Lamin B1 抗体 (C-12) 推荐用于 WB, IP, IF, IHC(P) 和 ELISA,检测mouse, rat 和human 来源的 Lamin B1; 也和以下物种反应,包括: canine
  • 抗Lamin B1抗体(C-12)可与琼脂糖结合用于IP;与HRP结合用于WB、IHC(P)和ELISA;与藻红蛋白FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取Lamin B1 (C-12): sc-365214免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP是Lamin B1 Antibody (C-12) 适用于 WB 和 IHC(P) 应用。 的首选辅助检测试剂。该试剂现与Lamin B1 Antibody (C-12) 搭配使用(请参阅下面的订购信息)。

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関連項目

Lamin B1抗体(C-12)是一种IgG2b κ小鼠单克隆Lamin B1抗体,可通过WB、IP、IF、IHC(P)和ELISA检测小鼠、大鼠和人类来源的Lamin B1。Lamin B1抗体(C-12)可以以非共轭抗Lamin B1抗体的形式提供,也可以以多种共轭形式的抗Lamin B1抗体提供,包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®共轭物。已发现一种独特的半胱氨酸蛋白酶家族,其序列、结构和底物特异性与先前描述的任何蛋白酶家族均不同。这个被称为Ced-3/ICE的家族是凋亡机制的关键组成部分,能够破坏对细胞寿命至关重要的特定靶蛋白。核纤层蛋白作为核纤层的一部分,对维持核膜和细胞形态的完整性至关重要。核纤层是核膜内核质侧的一层纤维层,被认为能够为核膜提供框架,也可能与染色质相互作用。B型核纤层蛋白会经历一系列修饰过程,如法尼基化和磷酸化。核纤层蛋白的磷酸化增加发生在核膜解体之前,可能在调节核纤层蛋白的关联中发挥作用。核纤层蛋白B在凋亡过程中被一个尚未确定的ICE家族成员所裂解。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

订购信息

产品名称产品编号规格价格数量收藏夹

Lamin B1 抗体 (C-12)

sc-365214
200 µg/ml
RMB2377.00

Lamin B1 (C-12): m-IgG Fc BP-HRP 套装

sc-525868
200 µg Ab; 10 µg BP
RMB2662.00

Lamin B1 抗体 (C-12) AC

sc-365214 AC
500 µg/ml, 25% agarose
RMB3129.00

Lamin B1 抗体 (C-12) HRP

sc-365214 HRP
200 µg/ml
RMB2377.00

Lamin B1 抗体 (C-12) FITC

sc-365214 FITC
200 µg/ml
RMB2482.00

Lamin B1 抗体 (C-12) PE

sc-365214 PE
200 µg/ml
RMB2580.00

Lamin B1 抗体 (C-12) Alexa Fluor® 488

sc-365214 AF488
200 µg/ml
RMB2685.00

Lamin B1 抗体 (C-12) Alexa Fluor® 546

sc-365214 AF546
200 µg/ml
RMB2685.00

Lamin B1 抗体 (C-12) Alexa Fluor® 594

sc-365214 AF594
200 µg/ml
RMB2685.00

Lamin B1 抗体 (C-12) Alexa Fluor® 647

sc-365214 AF647
200 µg/ml
RMB2685.00

Lamin B1 抗体 (C-12) Alexa Fluor® 680

sc-365214 AF680
200 µg/ml
RMB2685.00

Lamin B1 抗体 (C-12) Alexa Fluor® 790

sc-365214 AF790
200 µg/ml
RMB2685.00

Lamin B1 (C-12) 中和勝肽

sc-365214 P
100 µg/0.5 ml
RMB511.00

In Ihren Referenzabbildungen finden sich welche, bei denen 2 LaminB1 Banden sind.  Kann das eine modifizierte z.B. farnesylierte Form von LmnB1 sein? 

Asked by: Nestaubitz
Vielen Dank für Ihre Anfrage. Ja, Farnesylgruppen binden kovalent an die Cysteinylreste von Proteinen und können durchaus eine deutliche Doppelbande hervorrufen. Dieser Effekt kann auch von anderen posttranslationalen Modifikationen des Proteins hervorgerufen werden. The western blot analysis of Lamin B1 expression in PC-12 whole cell lysate (A) and rat brain tissue extract (B) shows two bands with the latter is sample. Can this be due to prenylation? Yes, prenylation is one of multiple possible post-translational modifications a protein may experience. Prenylation and other modifications can cause the migration behavior of the protein to be altered.
Answered by: SCBT Heidelberg Support
Date published: 2018-12-30

What application is the blocking peptide sc-365214 P appropriate for?

Asked by: jerojero
Thank you for your question. The blocking peptide is intended for use as a negative control, by pre-adsorbing the mouse monoclonal antibody against the antigen. For full protocol details, please contact our Technical Services Department or view our online protocol here: https://www.scbt.com/scbt/resources/protocols/peptide-neutralization
Answered by: Technical Support
Date published: 2017-02-24
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Rated 3 out of 5 by from This Ab is useful for human but not for mouse.I checked α-Lamin B1 antibodies from Santa Cruz (B-10, 8D1, A-11, C-5 and C-12). This antibody doesn't react with mouse cells. The intensity of signal is strong in human cell. But it also detect other bands.
Date published: 2021-11-10
Rated 5 out of 5 by from Very good antibody for nuclear extracts in WB!We've got a good signal in nuclear extracts from human microglia cell line
Date published: 2021-05-03
Rated 5 out of 5 by from Worked great!We used a Rat fibroblast cell line and we sometimes have to test a few antibodies to find one that works. So Santa Cruz's trial sample programs are great opportunity for us. We initially tested non-conjugated one and worked very well. We purchased conjugated version and it worked even better.
Date published: 2018-03-03
Rated 5 out of 5 by from Nice IF stain of nuclear membraneWe used this antibody for Immunofluorescence staining in HeLa cells. We were able to get nice signaling of the nuclear membrane with nice intensity
Date published: 2017-02-09
Rated 5 out of 5 by from Good quality antibody for human paraffin samplesGood quality antibody for human paraffin samples. Specific and sensitive -SCBT QC
Date published: 2015-02-25
Rated 4 out of 5 by from Works well for IF applicationsWorks well for IF applications, positive in HeLa. -SCBT QC
Date published: 2015-01-27
Rated 4 out of 5 by from Great monoclonal antibodyGreat monoclonal antibody! Specific bands in Y79 whole cell lysate. -SCBT QC
Date published: 2013-08-26
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Lamin B1 抗体 (C-12) is rated 4.4 out of 5 by 7.
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