Date published: 2025-9-6
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Lamin B1 Antibody (B-10) - Western Blotting - Image 392766
Lamin B1 抗体 (B-10) Alexa Fluor® 488: sc-374015 AF488. HL-60 (A), C2C12 (B), F9 (C) 和 CCRF-CEM (D) 全细胞裂解液中 Lamin B1 表达的直接荧光西部印迹分析. 用 UltraCruz® 阻断试剂: sc-516214 阻断. 用 Cruz Marker MW Tag-Alexa Fluor® 647 检测 Cruz Marker™ 分子量标准: sc-516791.
Lamin B1 抗体 (B-10) Alexa Fluor® 488: sc-374015 AF488. HL-60 (A), C2C12 (B), F9 (C) 和 CCRF-CEM (D) 全细胞裂解液中 Lamin B1 表达的直接荧光西部印迹分析. 用 UltraCruz® 阻断试剂: sc-516214 阻断. 用 Cruz Marker MW Tag-Alexa Fluor® 647 检测 Cruz Marker™ 分子量标准: sc-516791.

Lamin B1 抗体 (B-10): sc-374015

4.5(53)
寫評論提問

说明书
  • Lamin B1抗体(B-10)是小鼠单克隆IgG1 κ, 在314篇文献中引用，规格为200 µg/ml
  • 特异性抗原位于mouse物种的Lamin B11的C-terminus的氨基酸559-586之间
  • 抗-Lamin B1 抗体 (B-10) 推荐用于 WB, IP, IF 和 ELISA，检测mouse, rat 和human 来源的 Lamin B1
  • 抗Lamin B1抗体（B-10）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取Lamin B1 (B-10): sc-374015的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP和m-IgGκ BP-HRP是Lamin B1 Antibody (B-10) 适用于 WB 应用。 的首选辅助检测试剂，这些试剂现与Lamin B1 Antibody (B-10) 打包提供（请参阅下面的订购信息）。

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描述
基因信息
说明书与实验方案
研究信息

関連項目

额外Lamin抗体，包括Lamin A, Lamin B1, Lamin B2和Lamin C

Lamin B1抗体（B-10）是一种IgG1 κ小鼠单克隆Lamin B1抗体，可通过WB、IP、IF和ELISA检测小鼠、大鼠和人类来源的Lamin B1。Lamin B1抗体（B-10）可以以非共轭抗Lamin B1抗体的形式提供，也可以以多种共轭形式的抗Lamin B1抗体提供，包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®共轭物。已发现一种独特的半胱氨酸蛋白酶家族，其序列、结构和底物特异性与先前描述的任何蛋白酶家族均不同。这个被称为Ced-3/ICE的家族是凋亡机制的关键组成部分，能够破坏对细胞寿命至关重要的特定靶蛋白。核纤层蛋白作为核纤层的一部分，对维持核膜和细胞形态的完整性至关重要，核纤层是核膜内核质侧的一层纤维层，被认为能够为核膜提供框架，也可能与染色质相互作用。B型核纤层蛋白会经历一系列修饰过程，如法尼基化和磷酸化。核纤层蛋白的磷酸化增加发生在核膜解体之前，可能在调节核纤层蛋白关联中发挥作用。核纤层蛋白B在凋亡过程中被一个尚未确定的ICE家族成员所裂解。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

订购信息

产品名称产品编号规格价格数量收藏夹

Lamin B1 抗体 (B-10)

sc-374015
200 µg/ml
RMB2377.00

Lamin B1 (B-10): m-IgG Fc BP-HRP 套装

sc-529634
200 µg Ab; 10 µg BP
RMB2662.00

Lamin B1 (B-10): m-IgGκ BP-HRP 套装

sc-522588
200 µg Ab, 40 µg BP
RMB2662.00

Lamin B1 抗体 (B-10) AC

sc-374015 AC
500 µg/ml, 25% agarose
RMB3129.00

Lamin B1 抗体 (B-10) HRP

sc-374015 HRP
200 µg/ml
RMB2377.00

Lamin B1 抗体 (B-10) FITC

sc-374015 FITC
200 µg/ml
RMB2482.00

Lamin B1 抗体 (B-10) PE

sc-374015 PE
200 µg/ml
RMB2580.00

Lamin B1 抗体 (B-10) Alexa Fluor® 488

sc-374015 AF488
200 µg/ml
RMB2685.00

Lamin B1 抗体 (B-10) Alexa Fluor® 546

sc-374015 AF546
200 µg/ml
RMB2685.00

Lamin B1 抗体 (B-10) Alexa Fluor® 594

sc-374015 AF594
200 µg/ml
RMB2685.00

Lamin B1 抗体 (B-10) Alexa Fluor® 647

sc-374015 AF647
200 µg/ml
RMB2685.00

Lamin B1 抗体 (B-10) Alexa Fluor® 680

sc-374015 AF680
200 µg/ml
RMB2685.00

Lamin B1 抗体 (B-10) Alexa Fluor® 790

sc-374015 AF790
200 µg/ml
RMB2685.00

Lamin B1 (B-10) 中和勝肽

sc-374015 P
100 µg/0.5 ml
RMB511.00

Lamin B1 抗体 (B-10) sc-374015について。 200μg/mlで、量は何マイクロリットルでしょうか？

Asked by: okada
ご質問ありがとうございます。体積は1mlとなります。宜しくお願い致します。 Thank you for your question. The volume is 1 ml. Thank you for your cooperation.
Answered by: Jiajun Z
Date published: 2024-07-03

I will do fluorescent WB from subcellular fractionation. which Lamin B is suitable for me for nucleus HK?

Asked by: Medania
Thank you for your question. It would be helpful if you could call us directly at 800-457-3801ext.1, allowing for a more interactive discussion of this and other related questions.
Answered by: Blake J
Date published: 2021-11-23

Hi, do the directly labelled antibodies still have the Fc part. Indeed, one aim to use them would be to use another antibody made in mouse simultaneously in IF

Asked by: Clairec
Thank you for your question. Anti-Lamin B1 Antibody (B-10): sc-374015 is a complete mouse monoclonal IgG1 antibody with both Fab and Fc regions.
Answered by: Tech Support Europe
Date published: 2021-04-28

Before using this Ab, dose the extraction of nuclear proteins require specific steps for efficient detection of lamin B1

Asked by: jaou
Thank you for your question. You can follow our recommended protocol, which can be found under this link: https://www.scbt.com/de/resources/protocols/western-immuno-blotting
Answered by: Technical Support Europe
Date published: 2020-10-28

I used to do ChIP by using anti-LaminB (M-20) sc-6217 with Dynabeads protein G, and it worked well. Which magnetic beads do you recommend for ChIP using Lamin B1 Antibody (B-10): sc-374015 ?

Asked by: Naoko
Thank you for your question. This Lamin B1 antibody should work with any anti-mouse IgG magnetic beads. Since you have protein G magnetic beads, this should work fine since protein G has a strong binding to mouse IgG1. If you have any further questions, please contact our Asia Technical Service team. You can reach them by phone at (86 21) 6093-6350, by email at: [email protected] or by live chat directly on our website, www.scbt.com
Answered by: Tech Service
Date published: 2017-09-05

I'm trying to use this Ab for IF in dermal fibroblasts, so far with no results. I use methanol fixation at -20C for 5 min, 0,3% triton RT 3min, FBS blocking for 30 min and primary Ab incubation at 4C ON. Should I do anything different for it to work?

Asked by: Sofiamp
Thank you for your question. A link to our recommended protocol for IF using this mouse monoclonal antibody, Lamin B1 (B-10): sc-374015, is provided below. We recommend using the primary antibody at a starting dilution of 1:50. If after consulting our protocol you continue to have negative results, please contact our Technical Service Department for help with troubleshooting the antibody. Please call our European Support office toll-free at 00800 4573 8000, email [email protected], or contact us by live chat. https://www.scbt.com/scbt/resources/protocols/immunofluorescence-cell-staining
Answered by: Technical Support
Date published: 2017-11-21

I am using the LiCor/Odyssey system. Which secondary antibody do you recommend using with Lamin B1 Antibody (B-10): sc-374015?

Asked by: cjMara
We recommend using one of our our exclusive Mouse IgG Binding Proteins (m-IgGκ) conjugated to CFL 680 or CFL 790. They are suitable for use with Near-Infrared (NIR) detection systems, such as LI-COR/Odyssey and other comparable systems. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Answered by: Technical Support 10
Date published: 2017-01-09
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Rated 5 out of 5 by from Works great for WBWorks well on neonatal mouse lungs at concentration of 30ug. Antibody dilution will be 1:500 for overnight at 4oC
Date published: 2024-12-13
Rated 5 out of 5 by from Good for WBA clear band around the target size is detected in the nuclear isolation sample.
Date published: 2024-03-27
Rated 5 out of 5 by from Great for WBThe antibody is good for western blot. The band is single and clear.
Date published: 2024-03-19
Rated 5 out of 5 by from Ottimo funzionamento in IF!Ho provato questo anticorpo in immunofluorescenza e funziona molto bene con una marcatura definita a livello della membrana nucleare
Date published: 2023-11-30
Rated 5 out of 5 by from Clear Band in Western BlotsThe antibody worked nicely in Western Blot analyses of human cell lysates using dilutions at 1:500-1:1000.
Date published: 2023-11-25
Rated 4 out of 5 by from Works nicely for WBIt works nicely in total cellular lysates and fractionation experiments in MCF-7 cells.
Date published: 2023-10-18
Rated 4 out of 5 by from Good antibodyWorked well with western blotting at 1:500 dilution.
Date published: 2023-04-14
Rated 4 out of 5 by from Good workingI successfully detected murine Lamin B, but not human cells (A549).
Date published: 2023-04-13
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Lamin B1 抗体 (B-10) is rated 4.5 out of 5 by 53.
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