Date published: 2025-9-6
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Lamin B1 Antibody (8D1) - Western Blotting - Image 400707
Lamin B1 抗体 (8D1) HRP: sc-56144 HRP. CCRF-CEM (A), WR19L (B), MOLT-4 (C), Jurkat (D), Ramos (E) 和 NIH/3T3 (F) 全细胞裂解液中 Lamin B1 表达的直接 Western 印迹分析.
Lamin B1 抗体 (8D1) HRP: sc-56144 HRP. CCRF-CEM (A), WR19L (B), MOLT-4 (C), Jurkat (D), Ramos (E) 和 NIH/3T3 (F) 全细胞裂解液中 Lamin B1 表达的直接 Western 印迹分析.

Lamin B1 抗体 (8D1): sc-56144

4.8(12)
寫評論提問

说明书
  • Lamin B1抗体(8D1)是小鼠单克隆IgG1 κ, 在34篇文献中引用，规格为200 µg/ml
  • 针对从HeLahuman上皮样癌中纯化的核
  • 抗-Lamin B1 抗体 (8D1) 推荐用于 WB, IP, IF 和 IHC(P)，检测mouse, rat 和human 来源的 farnesylated and endoproteolysed Lamin B1; 不会与 Lamin B2产生交联反应
  • 抗Lamin B1抗体（8D1）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取Lamin B1 (8D1): sc-56144的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP 和 m-IgG1 BP-HRP 是 Lamin B1 抗体 (8D1) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与Lamin B1 抗体 (8D1) 组合成套装提供。 (请参阅下面的订购信息)。

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描述
基因信息
说明书与实验方案
研究信息

関連項目

此外 Lamin 抗体包括 Lamin A, Lamin B1, Lamin B2 和 Lamin C

Lamin B1 抗体 (8D1) 是一种小鼠单克隆 IgG1 kappa 轻链抗体，可通过免疫印迹 (WB)、免疫沉淀 (IP)、免疫荧光 (IF) 和免疫组化等应用检测小鼠、大鼠和人类样本中的 Lamin B1。抗维生素 B1 抗体 (8D1) 有非共轭型和多种共轭型，包括琼脂糖、辣根过氧化物酶 (HRP)、藻红蛋白 (PE)、异硫氰酸荧光素 (FITC) 和多种 Alexa Fluor® 共轭物。层蛋白 B1 是核薄层的重要组成部分，核薄层是一种纤维网，为核膜提供结构支持，在维持细胞形态方面发挥着重要作用。Lamin B1 对染色质组织和基因表达调控至关重要，因为 Lamin B1 与染色质和其他核蛋白相互作用，影响 DNA 复制和修复等细胞过程。Lamin B1 会发生翻译后修饰，包括法尼基化和磷酸化，这对其在细胞核内的功能和定位至关重要。编码层粘连蛋白 B1 的基因突变与常染色体显性成人型白质营养不良症（ALD）有关，ALD 是一种以进行性神经功能衰退和脱髓鞘为特征的疾病，强调了层粘连蛋白 B1 在维持神经完整性和功能方面的重要性。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

订购信息

产品名称产品编号规格价格数量收藏夹

Lamin B1 抗体 (8D1)

sc-56144
200 µg/ml
RMB2377.00

Lamin B1 (8D1): m-IgG Fc BP-HRP 套装

sc-526876
200 µg Ab; 10 µg BP
RMB2662.00

Lamin B1 (8D1): m-IgG1 BP-HRP 套装

sc-532249
200 µg Ab; 20 µg BP
RMB2662.00

Lamin B1 抗体 (8D1) AC

sc-56144 AC
500 µg/ml, 25% agarose
RMB3129.00

Lamin B1 抗体 (8D1) HRP

sc-56144 HRP
200 µg/ml
RMB2377.00

Lamin B1 抗体 (8D1) FITC

sc-56144 FITC
200 µg/ml
RMB2482.00

Lamin B1 抗体 (8D1) PE

sc-56144 PE
200 µg/ml
RMB2580.00

Lamin B1 抗体 (8D1) Alexa Fluor® 488

sc-56144 AF488
200 µg/ml
RMB2685.00

Lamin B1 抗体 (8D1) Alexa Fluor® 546

sc-56144 AF546
200 µg/ml
RMB2685.00

Lamin B1 抗体 (8D1) Alexa Fluor® 594

sc-56144 AF594
200 µg/ml
RMB2685.00

Lamin B1 抗体 (8D1) Alexa Fluor® 647

sc-56144 AF647
200 µg/ml
RMB2685.00

Lamin B1 抗体 (8D1) Alexa Fluor® 680

sc-56144 AF680
200 µg/ml
RMB2685.00

Lamin B1 抗体 (8D1) Alexa Fluor® 790

sc-56144 AF790
200 µg/ml
RMB2685.00

Please what is the price in AUD 

Asked by: Dr Magtouf Gatei
Thank you for contacting Santa Cruz Biotechnology. For a price quote and ship date please email [email protected].
Answered by: BlakeJ
Date published: 2025-07-28

Which is the epitope in Lamin B1 recognized by this antibody?

Asked by: cote13
Anti-Lamin B1 Antibody (8D1): sc-56144 was raised against nuclei purified from HeLa human epitheloid carcinoma. No epitope mapping has been performed for this antibody yet, so the exact binding site is not yet known.
Answered by: Tech Support Europe
Date published: 2021-06-25

I am using Lamin B1 (8D1): sc-56144 monoclonal antibody to stain paraffin embedded tissue. Do you recommend antigen retrieval when using this antibody?

Asked by: Germaine
We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-02-24
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Rated 5 out of 5 by from works well for WBWorks well on neonatal mouse lungs at concentration of 30ug. Antibody dilution will be 1:500 for overnight at 4oC
Date published: 2024-12-13
Rated 5 out of 5 by from よく検出できました。抗体サンプルをいただき、試しました。WBで良く検出できました。引き続き商品を購入し実験に使用する予定です。
Date published: 2022-04-18
Rated 5 out of 5 by from The antibody is very nice for WBLamin B1 was used as the loading control of nucles protein, its very nice for WB. The banding is very clear,I have introduce this product to my colleague. Thank you very much!
Date published: 2021-03-10
Rated 5 out of 5 by from Useful for IFI compared B-10 (sc-374015), A-11 (sc-377000) and 8D1 (sc-56144) for IF of both MEF wt and MEF Lmnb1-/-. B-10 is most brightest but 8D1 didn't labels other filaments in Lmnb1-/- background at neither -20C MeOH 10min or 4%PFA 15min fixation, followed 1:500 dilution / 4C overnight reaction.
Date published: 2020-09-04
Rated 5 out of 5 by from 功能很出色用这个抗体做核质分离的内参，非常好用，常温孵育1h，条带都很清晰，免疫荧光染色效果也和好，亮度很不错！
Date published: 2017-07-20
Rated 5 out of 5 by from specific band1:1000 conceration was used,so relatively cost effective. Very specific band at 67 kda without non-specifcbands.
Date published: 2017-07-08
Rated 5 out of 5 by from positive signalI run the western blot use Hela cell for positive control,I got strong signal.No background but strong band.
Date published: 2017-07-08
Rated 5 out of 5 by from Western BlotSatisfactory Western Blot data of Lamin B1 expression in human brain tissue,recommend use 1:1000 dilution for WB,
Date published: 2017-05-13
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Lamin B1 抗体 (8D1) is rated 4.8 out of 5 by 12.
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