Date published: 2025-9-6
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Lamin A/C Antibody (636) - Western Blotting - Image 390724
Lamin A/C 抗体 (636) Alexa Fluor® 594: sc-7292 AF594. C32 (A), PC-3 (B), HeLa (C) 和 A-431 (D) 全细胞裂解液中 Lamin A/C 表达的直接荧光西部印迹分析. 用 UltraCruz® 阻断试剂: sc-516214 阻断.
Lamin A/C 抗体 (636) Alexa Fluor® 594: sc-7292 AF594. C32 (A), PC-3 (B), HeLa (C) 和 A-431 (D) 全细胞裂解液中 Lamin A/C 表达的直接荧光西部印迹分析. 用 UltraCruz® 阻断试剂: sc-516214 阻断.

Lamin A/C 抗体 (636): sc-7292

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说明书
  • Lamin A/C抗体(636)是小鼠单克隆IgG2b κ, 在468篇文献中引用，规格为200 µg/ml
  • 免疫制备的猪核纤层蛋白
  • 抗-Lamin A/C 抗体 (636) 推荐用于 WB, IP, IF, IHC(P) 和 FCM，检测mouse, rat 和human 来源的 Lamin A and Lamin C
  • Lamin A/C抗体（636）可与琼脂糖结合用于IP，与藻红蛋白结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 594 用于IF, IHC(P) 和 FCM
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 可用于ChIP的TransCruz试剂(sc-7292 X, 200 µg/0.1 ml)
  • 关于如何获取Lamin A/C (636): sc-7292的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP 和 m-IgG2b BP-HRP 是 Lamin A/C 抗体 (636) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与Lamin A/C 抗体 (636) 组合成套装提供。 (请参阅下面的订购信息)。

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描述
基因信息
说明书与实验方案
研究信息

関連項目

额外Lamin抗体，包括Lamin A, Lamin B1, Lamin B2和Lamin C

Lamin A/C 抗体（636）是一种IgG2b κ小鼠单克隆Lamin A/C抗体，可通过WB、IP、IF、IHC(P)和FCM检测来自小鼠、大鼠和人的Lamin A和Lamin C。Lamin A/C抗体（636）有偶联和非偶联抗Lamin A/C抗体两种形式。已经被发现是一种独特的半胱氨酸蛋白酶家族，该家族在序列、结构和底物特异性方面与任何先前描述的蛋白酶家族不同。这个被称为Ced-3/ICE的家族由ICE、CPP32、ICH-1/Nedd-2、Tx、Mch2、Mch3（ICE-LAP3或CMH-1）、Mch4和ICE-LAP6组成。Ced-3/ICE家族成员作为凋亡机制的关键组成部分，可破坏对细胞寿命至关重要的特定目标蛋白。核纤层蛋白对于维持核膜完整性和细胞形态至关重要。核Lamin A被Mch2切割，但不被CPP32切割。核Lamin B因ICE家族中一个未识别的成员导致的凋亡而碎片化。Lamin C是Lamin A的剪接变体，仅在羧基端有所不同。Lamins A和C在前566个氨基酸中相同，而Lamin C仅在六个独特的羧基端氨基酸中有所不同。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

Lamin A/C 抗体 (636) 参考文献:

  1. Lamin A/C在骨髓间充质干细胞迁移和分化中的关键作用。  |  Alcorta-Sevillano, N., et al. 2020. Cells. 9: PMID: 32466483
  2. Lamin A/C和免疫系统：一种中间丝，多种功能。  |  Saez, A., et al. 2020. Int J Mol Sci. 21: PMID: 32854281
  3. 核包膜蛋白和中间丝蛋白在一级和二级结构上的同源性。  |  McKeon, FD., et al. Nature. 319: 463-8. PMID: 3453101
  4. 核片蛋白 A 和 C 的 cDNA 测序揭示了与中间丝蛋白的一级和二级结构同源性。  |  Fisher, DZ., et al. 1986. Proc Natl Acad Sci U S A. 83: 6450-4. PMID: 3462705
  5. Q353R-Lamin A/C诱发的TEAD1捕获会导致扩张型心肌病。  |  Yamada, S., et al. 2023. Sci Adv. 9: eade7047. PMID: 37058558
  6. DNA损伤通过ATR介导的层粘连蛋白A/C磷酸化诱导核膜破裂。  |  Kovacs, MT., et al. 2023. Mol Cell. 83: 3659-3668.e10. PMID: 37832547
  7. 核片层蛋白的动态特性和可能功能。  |  Moir, RD., et al. 1995. Int Rev Cytol. 162B: 141-82. PMID: 8557486
  8. ICE-LAP3是草履虫细胞死亡蛋白Ced-3的哺乳动物同源物，在Fas和肿瘤坏死因子诱导的细胞凋亡过程中被激活。  |  Duan, H., et al. 1996. J Biol Chem. 271: 1621-5. PMID: 8576161
  9. ICE-LAP6 是 ICE/Ced-3 基因家族的一个新成员，它被细胞毒性 T 细胞蛋白酶颗粒酶 B 激活。  |  Duan, H., et al. 1996. J Biol Chem. 271: 16720-4. PMID: 8663294
  10. Mch2 alpha 而非 CPP32 对层粘蛋白 A 的裂解：多种白细胞介素 1 β-转化酶相关蛋白酶在细胞凋亡过程中具有不同的底物识别特性。  |  Takahashi, A., et al. 1996. Proc Natl Acad Sci U S A. 93: 8395-400. PMID: 8710882
  11. Mch4体外激活CPP32和Mch3，Mch4是一种新型人类凋亡半胱氨酸蛋白酶，含有两个类似FADD的结构域。  |  Fernandes-Alnemri, T., et al. 1996. Proc Natl Acad Sci U S A. 93: 7464-9. PMID: 8755496
  12. 凋亡过程中，板层蛋白酶解促进了核事件的发生。  |  Rao, L., et al. 1996. J Cell Biol. 135: 1441-55. PMID: 8978814

订购信息

产品名称产品编号规格价格数量收藏夹

Lamin A/C 抗体 (636)

sc-7292
200 µg/ml
RMB2377.00

Lamin A/C (636): m-IgG Fc BP-HRP 套装

sc-525435
200 µg Ab; 10 µg BP
RMB2662.00

Lamin A/C (636): m-IgG2b BP-HRP 套装

sc-549562
200 µg Ab; 10 µg BP
RMB2662.00

Lamin A/C 抗体 (636) X

sc-7292 X
200 µg/0.1 ml
RMB2377.00

Lamin A/C 抗体 (636) AC

sc-7292 AC
500 µg/ml, 25% agarose
RMB3129.00

Lamin A/C 抗体 (636) PE

sc-7292 PE
200 µg/ml
RMB2580.00

Lamin A/C 抗体 (636) Alexa Fluor® 546

sc-7292 AF546
200 µg/ml
RMB2685.00

Lamin A/C 抗体 (636) Alexa Fluor® 594

sc-7292 AF594
200 µg/ml
RMB2685.00

Lamin A/C 抗体 (636) Alexa Fluor® 680

sc-7292 AF680
200 µg/ml
RMB2685.00

Lamin A/C 抗体 (636) Alexa Fluor® 790

sc-7292 AF790
200 µg/ml
RMB2685.00

would you be able to tell me if this antibody was raised against the full length porcine lamin preparation and where if known the specific region the antibody is recognizing? many thanks Steve

Asked by: Steve
Thank you for your question. sc-7292 Lamin A/C (636) has not been epitope mapped. Here is a link for more information on the protein. https://www.uniprot.org/uniprotkb/P02545/entry
Answered by: BlakeJ
Date published: 2025-07-28

Hello, does this antibody recognise N-terminal or C-terminal part of the protein? Best wishes

Asked by: npnp22
Thank you for your question. No epitope mapping has been performed for this antibody as of yet, so its binding site is not known at this point.
Answered by: Tech Support Europe
Date published: 2023-02-28

What is the difference in specificity and efficacy or advantage/disadvantage of this product (sc-7292) compared to the Anti-Lamin A/C Antibody (E-1): sc-376248?

Asked by: missy
Thank you for your question. Anti-Lamin A/C Antibody (636) is a mouse monoclonal IgG2b κ recommended for detection of Lamin A and Lamin C of mouse, rat and human origin by WB, IP, IF, IHC(P) and FCM. Anti-Lamin A/C Antibody (E-1) is a mouse monoclonal IgG1 κ recommended for detection of Lamin A and Lamin C of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA; also reactive with additional species, including and equine, canine, bovine and porcine. If you are intending to use one or the other for detection of Lamin A and C from mouse, rat, or human in WB, IP, IF, or IHC(P), either antibody would perform well and with very similar results.
Answered by: Tech Support Europe
Date published: 2021-11-08

Can this antibody be used to detect claeved Lamin A / C?

Asked by: Warner
Thank you for your question. It would be helpful if you could call us, allowing for a more interactive discussion of this and other related questions.
Answered by: Technical Support
Date published: 2021-07-23

I used in the past Ab sc-376248 which is no longer available. I would like to test sc-7292 to verify if it is a valid substitute.

Asked by: GigiR
Thank you for your question. Our website was having technical issues today. sc-376248 is definitely still available and you can continue using this antibody. Please reach out to your distributor for information if you still would like to find out how to get a test sample of a different antibody.
Answered by: Tech Service
Date published: 2020-02-07

Can you send me the MSDS/SDS for the following product? Lamin A/C Antibody (636): sc-7292

Asked by: Lozinski
Thank you for your question. This antibody, sc-7292, Lamin A/C (636), is produced by Santa Cruz Biotechnology and is a non-hazardous products for which no SDS is required. Our primary antibodies are provided in a solution of 1x PBS, 0.1% sodium azide, and 0.1% gelatin. You can find the datasheet for this product by following the link below: https://datasheets.scbt.com/sc-7292.pdf Please contact Technical Service by phone, (800)-457-3801 option 2, email <[email protected]>, or by live chat directly on our website, www.scbt.com if you have any further questions.
Answered by: Tech Service
Date published: 2017-04-03

What is the recommended secondary reagent to be used with this mouse monoclonal primary for WB?

Asked by: Cweed
We recommend using one our exclusive Mouse IgG Binding Proteins as a secondary detection reagent. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Answered by: TechService7
Date published: 2016-12-16

I need to use an antibody to detect Lamin in porcine samples. Do you know if this mouse monoclonal antibody will work?

Asked by: Cweed
Please contact our Technical Service Department and we would be happy to perform sequence analysis and review our data to answer any questions you may have about additional species reactivity.
Answered by: TechService7
Date published: 2016-12-16
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Rated 5 out of 5 by from good for westernI am glad I bought this a few weeks ago. I did western with it. the band is good.
Date published: 2022-02-23
Rated 3 out of 5 by from Not suitable for mouse IFI compared to other company's mouse mAb for IF of both MEF wt and MEF LmnA/C-/-. 636 is not capable of IF with mouse embryonic fibroblasts in our condition (4%PFA 15min fixation followed 1:500 dilution / 4C overnight reaction).
Date published: 2021-01-30
Rated 5 out of 5 by from Great signalIt shows a great clean signal in western blot studies of nuclear extracts from rat liver samples
Date published: 2021-01-18
Rated 5 out of 5 by from Great bandsDillution 1/250 and it worked great in western blot!
Date published: 2019-04-01
Rated 5 out of 5 by from works very wellQuality is great and the price is very good given that all the items needed. It worked well with 1:500 dilution factor and for whole cell and cytoplasmic lysis
Date published: 2019-03-10
Rated 5 out of 5 by from nice and clear signalIt showed a clear and strong signal in WB of canine samples.
Date published: 2017-11-06
Rated 5 out of 5 by from work wellwork well in wb at 1:1000, HCT116 cells, SW480 cells
Date published: 2017-10-16
Rated 5 out of 5 by from Great signalI have used the Lamin A/C antibody for western blotting (1:500 dilutioin) and I get a good clean signal. I have used this for detection with nuclear extracts and cytoplasmic extracts and I get a clean signal in my nuclear extracts.
Date published: 2017-08-25
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Lamin A/C抗体 (636) is rated 4.9 out of 5 by 16.
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