Date published: 2025-9-9
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LAL Antibody (9G7F12) - Western Blotting - Image 15568
LAL 抗体 (9G7F12): sc-58374. 人脑 (A), 人肺 (B) 和人脾 (C) 组织提取物以及 HeLa (D) 全细胞裂解液中 LAL 表达的 Western 印迹分析.
LAL 抗体 (9G7F12): sc-58374. 人脑 (A), 人肺 (B) 和人脾 (C) 组织提取物以及 HeLa (D) 全细胞裂解液中 LAL 表达的 Western 印迹分析.

LAL 抗体 (9G7F12): sc-58374

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说明书
  • LAL 抗体 9G7F12 是小鼠单克隆 IgG1 κ，LAL抗体, 在6篇文献中引用，规格为200 µg/ml
  • 针对纯化的截短重组human的LAL
  • LAL 抗体 (9G7F12) 推荐用于 WB, IP, IF 和 IHC(P)，检测mouse, rat 和human 来源的 Lipase A
  • 抗LAL抗体（9G7F12）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取LAL (9G7F12): sc-58374的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgGκ BP-HRP 是 LAL 抗体 (9G7F12) 在 WB and IHC(P) 应用中的首选二抗检测试剂。该试剂现与LAL 抗体 (9G7F12) 组合成套装提供。 (请参阅下面的订购信息)。其他m-IgGκ BP 偶联产品，请参阅我们的小鼠 IgG 结合蛋白完整列表。

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描述
基因信息
说明书与实验方案
研究信息

関連項目

LAL 抗体（9G7F12）是一种小鼠单克隆 IgG1 kappa 轻链抗体，可通过免疫印迹（WB）、免疫沉淀（IP）、免疫荧光（IF）和免疫组织化学方法检测小鼠、大鼠和人源的 LAL 蛋白。抗 LAL 抗体 (9G7F12) 有非共轭型和多种共轭型，包括琼脂糖、辣根过氧化物酶 (HRP)、藻红蛋白 (PE)、异硫氰酸荧光素 (FITC) 和多种 Alexa Fluor® 共轭物。LAL 蛋白是脂肪酶基因家族的成员，在脂质代谢中，特别是在溶酶体内水解甘油三酯和胆固醇酯的过程中发挥着重要作用。LAL 酶活性对维持脂质平衡至关重要，LAL 基因缺陷可导致严重的代谢紊乱，如沃尔曼病和胆固醇酯贮积症。LAL 有六个潜在的 N-糖基化位点和一个潜在的 O-糖基化位点，这些位点对稳定性和功能非常重要，因为这些翻译后修饰可影响酶的活性和底物识别。LAL 的过量表达也与动脉粥样硬化的发生有关，这凸显了它在正常生理和疾病状态下的重要性。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

LAL 抗体 (9G7F12) 参考文献:

  1. 溶酶体酸脂肪酶缺陷小鼠：白色和棕色脂肪减少, 肝脾肿大严重, 寿命缩短。  |  Du, H., et al. 2001. J Lipid Res. 42: 489-500. PMID: 11290820
  2. 小鼠溶酶体酸性脂肪酶缺乏症的酶疗法。  |  Du, H., et al. 2001. Hum Mol Genet. 10: 1639-48. PMID: 11487567
  3. α/β水解酶折叠。  |  Ollis, DL., et al. 1992. Protein Eng. 5: 197-211. PMID: 1409539
  4. 在小鼠裂殖酵母中生产重组人溶酶体酸性脂肪酶：开发一种饲料批量发酵和纯化工艺。  |  Ikeda, S., et al. 2004. J Biosci Bioeng. 98: 366-73. PMID: 16233721
  5. 用洛伐他汀和依折麦布治疗一名患有胆固醇酯贮积症的青少年的血脂异常。  |  Tadiboyina, VT., et al. 2005. Lipids Health Dis. 4: 26. PMID: 16255772
  6. TRP-ML1 可调节溶酶体 pH 值和酸性溶酶体脂质水解活性。  |  Soyombo, AA., et al. 2006. J Biol Chem. 281: 7294-301. PMID: 16361256
  7. 甘露糖化酶和甘露糖受体在酶替代疗法中的作用。  |  Du, H., et al. 2005. Am J Hum Genet. 77: 1061-74. PMID: 16380916
  8. 每种脂肪酶对有机磷抑制剂都有独特的敏感性。  |  Quistad, GB., et al. 2006. Toxicol Sci. 91: 166-72. PMID: 16449251
  9. 自噬通过溶酶体酸性脂肪酶调节巨噬细胞泡沫细胞中的胆固醇外排。  |  Ouimet, M., et al. 2011. Cell Metab. 13: 655-67. PMID: 21641547
  10. 脂质代谢及其他方面的溶酶体酸性脂肪酶。  |  Li, F. and Zhang, H. 2019. Arterioscler Thromb Vasc Biol. 39: 850-856. PMID: 30866656
  11. 对溶酶体酸性脂肪酶缺乏症的最新见解。  |  Korbelius, M., et al. 2023. Trends Mol Med. 29: 425-438. PMID: 37028992
  12. 小鼠溶酶体酸性脂肪酶基因的靶向干扰：大量胆固醇酯和甘油三酯贮存的长期存活。  |  Du, H., et al. 1998. Hum Mol Genet. 7: 1347-54. PMID: 9700186

订购信息

产品名称产品编号规格价格数量收藏夹

LAL 抗体 (9G7F12)

sc-58374
200 µg/ml
RMB2377.00

LAL (9G7F12): m-IgGκ BP-HRP 套装

sc-521054
200 µg Ab, 40 µg BP
RMB2662.00

LAL 抗体 (9G7F12) AC

sc-58374 AC
500 µg/ml, 25% agarose
RMB3129.00

LAL 抗体 (9G7F12) HRP

sc-58374 HRP
200 µg/ml
RMB2377.00

LAL 抗体 (9G7F12) FITC

sc-58374 FITC
200 µg/ml
RMB2482.00

LAL 抗体 (9G7F12) PE

sc-58374 PE
200 µg/ml
RMB2580.00

LAL 抗体 (9G7F12) Alexa Fluor® 488

sc-58374 AF488
200 µg/ml
RMB2685.00

LAL 抗体 (9G7F12) Alexa Fluor® 546

sc-58374 AF546
200 µg/ml
RMB2685.00

LAL 抗体 (9G7F12) Alexa Fluor® 594

sc-58374 AF594
200 µg/ml
RMB2685.00

LAL 抗体 (9G7F12) Alexa Fluor® 647

sc-58374 AF647
200 µg/ml
RMB2685.00

LAL 抗体 (9G7F12) Alexa Fluor® 680

sc-58374 AF680
200 µg/ml
RMB2685.00

LAL 抗体 (9G7F12) Alexa Fluor® 790

sc-58374 AF790
200 µg/ml
RMB2685.00

Hello, We bought the anti LIPA monoclonal antibody 9G7F12 and wouldlike to know what etpitope the antibody recognize and also I the antibody has been raised. We thank you very much for your supoport.

Asked by: Matthias12
Thank you for your question. It would be helpful if you could call us directly at 800-457-3801 ext. 1, allowing for a more interactive discussion of this and other related questions.
Answered by: Blake J
Date published: 2021-09-22

Hi, I purchased this antibody and used it on mouse cell line 3T3-l1. When doing a western blot I found a surprisingly strong signal around 100kDa and a weak signal around 50kDa.  Do you have any info if the 100kDa is LAL? Thanks,

Asked by: Ofer Moldavski
Thank you for your question. The predicted molecular weight of LAL is ~ 45 kDa. We do not have any data or information regarding a 100 kDa form of LAL. We also have not specifically tested this antibody in 3T3-L1 cell lysate. Please contact our Technical Service Department for support in troubleshooting this antibody.
Answered by: Technical Support
Date published: 2019-04-23

Can LAL (9G7F12): sc-58374 monoclonal antibody be used in IF or IHC with mouse tissue or cells? Will there be non-specific staining?

Asked by: Trav11
Thank you for your inquiry. The use of mouse monoclonal antibodies with mouse samples is very common and typically poses no problem. To eliminate any potential cross-reactivity of an anti-mouse secondary detection reagent, LAL (9G7F12): sc-58374 is available in a variety of direct conjugations, such as HRP, PE, FITC and AlexaFluors.
Answered by: Technical Support
Date published: 2017-02-24
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Rated 1 out of 5 by from 9G7F12 AC in IP of LAL protein in HepG2This antibody yields a lot of unspecific bands and the immunoprecipitation is not pure at all. It seems that the proteins stick to the beads. In contrast, the same antibody unconjugated is much better an yields much more pure sample in immunoprecipitation. Based on this, we would like to ask for refund
Date published: 2022-09-20
Rated 4 out of 5 by from IHC-PIt could work in human samples by IHC-P............
Date published: 2017-02-10
Rated 5 out of 5 by from Higly recommended for IHC(P)Beautiful cytoplasmic immunoperoxidase staining of formalin fixed, paraffin-embedded human duodenum tissue and human spleen.
Date published: 2017-02-01
Rated 3 out of 5 by from Works well in WBDetects a single band at a 1:250 dilution in HeLa cells.
Date published: 2016-11-29
Rated 5 out of 5 by from Produced exceptional immunoperoxidase cytoplasmicProduced exceptional immunoperoxidase cytoplasmic staining in formalin fixed, paraffin-embedded human small intestine tissue. -SCBT QC
Date published: 2015-02-16
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LAL 抗体 (9G7F12) is rated 3.8 out of 5 by 6.
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