Date published: 2025-9-8

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L3MBTL 抗体 (D-9): sc-166905

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说明书
  • L3MBTL 抗体 D-9 是小鼠单克隆 IgG2a κ,L3MBTL抗体, 在1篇文献中引用,规格为200 µg/ml
  • 特异性抗原位于human物种的L3MBTL的内部区域内的氨基酸408-425之间
  • L3MBTL 抗体 (D-9) 推荐用于WB, IP, IF 和 ELISA,检测human 来源的 L3MBTL
  • 抗L3MBTL抗体(D-9)可与琼脂糖结合用于IP;与HRP结合用于WB、IHC(P)和ELISA;与藻红蛋白FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取L3MBTL (D-9): sc-166905免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgGκ BP-HRP是L3MBTL Antibody (D-9) 适用于 WB 应用。 的首选辅助检测试剂。该试剂现可与L3MBTL Antibody (D-9) 搭配使用(请参阅下面的订购信息)。有关其它 m-IgGκ BP 结合物,请参阅我们完整的小鼠 IgG 结合蛋白列表。

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関連項目

L3MBTL抗体(D-9)是一种IgG2a κ小鼠单克隆L3MBTL抗体(也称为L3MBTL抗体),可通过WB、IP、IF和ELISA检测人源L3MBTL蛋白。L3MBTL抗体(D-9)既可以是非偶联的抗L3MBTL抗体形式,也可以是多种偶联形式的抗L3MBTL抗体,包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®偶联物。L3MBTL是Polycomb蛋白家族的成员,在大蛋白复合物中作为转录抑制因子发挥作用。L3MBTL包含三个称为MBT重复的100个残基重复序列,以及一个由芳香族氨基酸排列的空腔内的C端α螺旋结构。该蛋白在造血组织中经历单等位基因甲基化,并在大多数人类成年正常组织中表达。在间期,L3MBTL定位于细胞核,并与有丝分裂细胞中的凝缩染色体完全结合。L3MBTL与Trithorax组蛋白共同负责基因活性的协调调控。人类L3MBTL基因位于20号染色体上的一个区域,该区域在髓系恶性肿瘤患者中经常被删除,已被提议作为候选的20q肿瘤抑制基因,暗示L3MBTL在某些髓系白血病病例中的表达。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

L3MBTL 抗体 (D-9) 参考文献:

  1. 果蝇致死(3)恶性脑瘤(l(3)mbt)蛋白的人类同源物与有丝分裂染色体缔合作用。  |  Koga, H., et al. 1999. Oncogene. 18: 3799-809. PMID: 10445843
  2. 人类 L(3)MBT 多能团蛋白是一种转录抑制因子,与 TEL(ETV6)存在物理和官能团上的相互作用。  |  Boccuni, P., et al. 2003. J Biol Chem. 278: 15412-20. PMID: 12588862
  3. 恶性脑肿瘤重复序列:带有配体/肽结合口袋的三叶螺旋桨结构。  |  Wang, WK., et al. 2003. Structure. 11: 775-89. PMID: 12842041
  4. 类中性结合蛋白 2(SCML2)中恶性脑肿瘤(MBT)重复序列的晶体结构。  |  Sathyamurthy, A., et al. 2003. J Biol Chem. 278: 46968-73. PMID: 12952983
  5. l(3)恶性脑瘤和果蝇生殖细胞形成所需的三个新基因  |  Yohn, CB., et al. 2003. Genetics. 165: 1889-900. PMID: 14704174
  6. 人类 L3MBTL 基因的印迹,该基因是位于人类骨髓恶性肿瘤中被删除的 20 号染色体区域的多聚酶家族成员。  |  Li, J., et al. 2004. Proc Natl Acad Sci U S A. 101: 7341-6. PMID: 15123827
  7. 正常和恶性造血细胞中 L3MBTL 基因的结构完整性和表达。  |  MacGrogan, D., et al. 2004. Genes Chromosomes Cancer. 41: 203-13. PMID: 15334543
  8. 髓系恶性肿瘤患者中的印迹多聚酶基因 L3MBTL(一种候选的 20q 肿瘤抑制基因)的特征。  |  Bench, AJ., et al. 2004. Br J Haematol. 127: 509-18. PMID: 15566354
  9. L3MBtl是印迹 L3MBTL 的小鼠直向同源物,它显示出复杂的替代剪接模式,并逃避基因组印迹。  |  Li, J., et al. 2005. Genomics. 86: 489-94. PMID: 16081246

订购信息

产品名称产品编号规格价格数量收藏夹

L3MBTL 抗体 (D-9)

sc-166905
200 µg/ml
RMB2377.00

L3MBTL (D-9): m-IgGκ BP-HRP 套装

sc-521792
200 µg Ab, 40 µg BP
RMB2662.00

L3MBTL 抗体 (D-9) AC

sc-166905 AC
500 µg/ml, 25% agarose
RMB3129.00

L3MBTL 抗体 (D-9) HRP

sc-166905 HRP
200 µg/ml
RMB2377.00

L3MBTL 抗体 (D-9) FITC

sc-166905 FITC
200 µg/ml
RMB2482.00

L3MBTL 抗体 (D-9) PE

sc-166905 PE
200 µg/ml
RMB2580.00

L3MBTL 抗体 (D-9) Alexa Fluor® 488

sc-166905 AF488
200 µg/ml
RMB2685.00

L3MBTL 抗体 (D-9) Alexa Fluor® 546

sc-166905 AF546
200 µg/ml
RMB2685.00

L3MBTL 抗体 (D-9) Alexa Fluor® 594

sc-166905 AF594
200 µg/ml
RMB2685.00

L3MBTL 抗体 (D-9) Alexa Fluor® 647

sc-166905 AF647
200 µg/ml
RMB2685.00

L3MBTL 抗体 (D-9) Alexa Fluor® 680

sc-166905 AF680
200 µg/ml
RMB2685.00

L3MBTL 抗体 (D-9) Alexa Fluor® 790

sc-166905 AF790
200 µg/ml
RMB2685.00

L3MBTL (D-9) 中和勝肽

sc-166905 P
100 µg/0.5 ml
RMB511.00

I wonder which L3MBTL paralog this antibody recognizes. According to Uniprot there are 4 L3MBTL proteins with comparable sizes. Thanks for the reply,

Asked by: aktanalp
Thank you for your question. This mouse monoclonal, L3MBTL (D-9): sc-166905 is specific for an epitope mapping between amino acids 408-425 within an internal region of L3MBTL of human origin. According to Uniprot, (Q9Y468) this antibody has an epitope that is conserved in all 5 of the described isoforms of human L3MBTL.
Answered by: Technical Support
Date published: 2017-05-16

What application is the blocking peptide sc-166905 P appropriate for?

Asked by: Germaine
Thank you for your question. The blocking peptide is intended for use as a negative control, by pre-adsorbing the mouse monoclonal antibody against the antigen. For full protocol details, please contact our Technical Services Department or view our online protocol here: https://www.scbt.com/scbt/resources/protocols/peptide-neutralization
Answered by: Technical Support
Date published: 2017-02-27
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Rated 4 out of 5 by from IP and WB data was goodIP and WB data was good. no issues.
Date published: 2015-09-10
Rated 5 out of 5 by from Satisfactory Western Blot data of L3MBTLSatisfactory Western Blot data of L3MBTL expression in U-87 MG whole cell lysate. -SCBT QC
Date published: 2015-02-23
Rated 5 out of 5 by from Great Western Blot data of L3MBTL expressionGreat Western Blot data of L3MBTL expression in non-transfected and human L3MBTL transfected 293T whole cell lysates. -SCBT QC
Date published: 2013-09-18
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L3MBTL 抗体 (D-9) is rated 4.7 out of 5 by 3.
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