Date published: 2026-10-24

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Ku70 Antibody (N3H10) - Western Blotting - Image 15529
Ku70 抗体 (N3H10): sc-56129. K-562 (A), HeLa (B) 和 C32 (C) 全细胞裂解液中 Ku70 表达的 Western 印迹分析.
Ku70 抗体 (N3H10): sc-56129. K-562 (A), HeLa (B) 和 C32 (C) 全细胞裂解液中 Ku70 表达的 Western 印迹分析.

Ku70 抗体 (N3H10): sc-56129

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寫評論提問
说明书
  • Ku70抗体(N3H10)是小鼠单克隆IgG2b κ, 在22篇文献中引用，规格为200 µg/ml
  • 针对PSE1-PL human胎盘细胞
  • Ku70 抗体 (N3H10) 推荐用于WB, IP, IF 和 IHC(P) ，检测 mouse, rat, human 和 Xenopus来源的Ku70
  • 抗Ku70抗体（N3H10）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • m-IgG Fc BP-HRP和 2b BP-HRP">m-IgG2b BP-HRP是Ku70 Antibody (N3H10) 适用于 WB 和 IHC(P) 应用。 的首选辅助检测试剂，这些试剂现与Ku70 Antibody (N3H10) 打包提供（请参阅下面的订购信息）。
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関連項目

附加Ku Lupus Antigen抗体，包括Ku70和Ku86

Ku70抗体（N3H10）是一种小鼠单克隆IgG2b kappa轻链抗体，可通过蛋白质印迹（WB）、免疫沉淀（IP）、免疫荧光（IF）和免疫组织化学等方法检测小鼠、大鼠和人类样本中的Ku70。抗Ku70抗体（N3H10）有非结合型和多种结合型，包括琼脂糖、辣根过氧化物酶（HRP）、藻红蛋白（PE）、异硫氰酸荧光素（FITC）和多种Alexa Fluor®结合物。Ku70是Ku异二聚体的重要组成部分，与Ku86一起在细胞对DNA损伤的反应中发挥着至关重要的作用，特别是在非同源末端连接（NHEJ）修复途径中。该途径通过修复DNA双链断裂来维持基因组的稳定性，而DNA双链断裂可能由电离辐射和氧化应激等多种因素引起。Ku70与DNA末端结合有助于修复因子的募集，并保护DNA免受降解。Ku70还调节转录和细胞信号传导，在细胞过程中发挥着多方面的作用。Ku70 (N3H10)抗体使研究人员能够研究DNA修复机制及其在癌症等疾病中的影响，因为DNA修复途径在癌症中经常失调。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

Ku70 抗体 (N3H10) 参考文献:

  1. Ku70 在 Mcl-1 的去泛素化和抑制细胞凋亡中的作用  |  Wang, B., et al. 2014. Cell Death Differ. 21: 1160-9. PMID: 24769731
  2. Ku70是组蛋白去乙酰化酶抑制剂trichostatin A诱导细胞凋亡的关键。  |  Meng, J., et al. 2015. Mol Med Rep. 12: 581-6. PMID: 25695595
  3. Bax诱导的细胞凋亡通过诱发肺气肿缩短了DNA修复缺陷Ku70基因敲除小鼠的寿命。  |  Matsuyama, S., et al. 2016. Exp Biol Med (Maywood). 241: 1265-71. PMID: 27302174
  4. Ku70和Ku80参与人类巨噬细胞和单核细胞中LPS诱导的促炎细胞因子产生。  |  Sun, H., et al. 2020. Aging (Albany NY). 12: 20432-20444. PMID: 33109771
  5. Ku70调节减轻小鼠过敏性哮喘特征并恢复肺部线粒体功能。  |  Rehman, R., et al. 2021. Mitochondrion. 57: 76-87. PMID: 33359362
  6. ATG5与Ku70的新型相互作用可在基因毒性应激时促进DNA修复。  |  Demirbag-Sarikaya, S., et al. 2022. Sci Rep. 12: 8134. PMID: 35581289
  7. Ku70影响辐射后人类淋巴细胞和T细胞急性淋巴细胞白血病中染色体易位的频率。  |  Cheng, Z., et al. 2022. Radiat Oncol. 17: 144. PMID: 35986335
  8. Ku70核定位信号的乙酰化可减少与importin-α的相互作用。  |  Fujimoto, H., et al. 2023. Biochem Biophys Rep. 33: 101418. PMID: 36620088
  9. Ku70 S155磷酸化特异性BioID2相互作用组分析鉴定了Ku与TRIP12在DNA损伤反应中的关联。  |  Abbasi, S., et al. 2023. Int J Mol Sci. 24: PMID: 37108203
  10. (-)-表没食子儿茶素-3-没食子酸酯通过分离胃癌细胞中的c-FLIP/Ku70复合物诱导细胞凋亡。  |  Shahriari Felordi, M., et al. 2023. J Cell Mol Med. 27: 2572-2582. PMID: 37537749

订购信息

产品名称产品编号规格价格数量收藏夹

Ku70 抗体 (N3H10)

sc-56129
200 µg/ml
CNY2422.00

Ku70 (N3H10): m-IgG Fc BP-HRP 套装

sc-525649
200 µg Ab; 10 µg BP
CNY2715.00

Ku70 (N3H10): m-IgG2b BP-HRP 套装

sc-549605
200 µg Ab; 10 µg BP
CNY2715.00

Ku70 抗体 (N3H10) AC

sc-56129 AC
500 µg/ml, 25% agarose
CNY3189.00

Ku70 抗体 (N3H10) HRP

sc-56129 HRP
200 µg/ml
CNY2422.00

Ku70 抗体 (N3H10) FITC

sc-56129 FITC
200 µg/ml
CNY2527.00

Ku70 抗体 (N3H10) PE

sc-56129 PE
200 µg/ml
CNY2625.00

Ku70 抗体 (N3H10) Alexa Fluor® 488

sc-56129 AF488
200 µg/ml
CNY2738.00

Ku70 抗体 (N3H10) Alexa Fluor® 546

sc-56129 AF546
200 µg/ml
CNY2738.00

Ku70 抗体 (N3H10) Alexa Fluor® 594

sc-56129 AF594
200 µg/ml
CNY2738.00

Ku70 抗体 (N3H10) Alexa Fluor® 647

sc-56129 AF647
200 µg/ml
CNY2738.00

Ku70 抗体 (N3H10) Alexa Fluor® 680

sc-56129 AF680
200 µg/ml
CNY2738.00

Ku70 抗体 (N3H10) Alexa Fluor® 790

sc-56129 AF790
200 µg/ml
CNY2738.00

I would like to request a FREE 10 µg sample. How can I proceed? Thanks, Jacky

Asked by: Jacky01
Thank you for your question. Please contact Technical Service for more information on how to receive a 10 μg sample of our well characterized monoclonal antibody.
Answered by: Technical Support
Date published: 2018-12-05

I am using Ku-70 (N3H10): sc-56129 monoclonal antibody to stain paraffin embedded tissue. Do you recommend antigen retrieval when using this antibody?

Asked by: cjMara
We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-02-24
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Rated 5 out of 5 by jenniferc from Better than PolyThis monoclonal antibody produced strong bands with no background. IHCP stain was nice and specific
Date published: 2017-01-26
Rated 5 out of 5 by HF from Good specificity shown by using transfectedGood specificity shown by using transfected and non-transfected 293T cells for over-expression of the human Ku-70 protein via Western Blot analysis. -SCBT QC
Date published: 2015-05-18
Rated 5 out of 5 by JM from Various publications support the use of thisVarious publications support the use of this antibody in WB using human embryonic kidney (HEK) 293T, normal human fibroblast strains (MRC-5 and WI-38), human osteosarcoma strain (U-2 OS), human lung adenocarcinoma cell line A549 and p53-deficient lung carcinoma H1299 cells. -SCBT Publication Review
Date published: 2015-04-25
Rated 5 out of 5 by JM from It has been reported in literature that thisIt has been reported in literature that this antibody is suitable for use in WB using Xenopus sperm. -SCBT Publication Review
Date published: 2015-03-24
Rated 5 out of 5 by ET from Publishable data obtained by IP and WB usingPublishable data obtained by IP and WB using human H358 and H322 NSCLC cell lines. -SCBT Publication Review
Date published: 2015-03-14
Rated 5 out of 5 by LF from Positive detection of endogenous expression of KuPositive detection of endogenous expression of Ku-70 protein by WB in K-562, HeLa and C32 cell lines. -SCBT QC
Date published: 2014-07-15
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Ku70 抗体 (N3H10) is rated 5.0 out of 5 by 6.
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