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Ku70 Antibody (N3H10) - Western Blotting - Image 15529
Ku70 抗体 (N3H10): sc-56129. K-562 (A), HeLa (B) 和 C32 (C) 全细胞裂解液中 Ku70 表达的 Western 印迹分析.
Ku70 抗体 (N3H10): sc-56129. K-562 (A), HeLa (B) 和 C32 (C) 全细胞裂解液中 Ku70 表达的 Western 印迹分析.

Ku70 抗体 (N3H10): sc-56129

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寫評論提問

说明书
  • Ku70抗体(N3H10)是小鼠单克隆IgG2b κ, 在22篇文献中引用，规格为200 µg/ml
  • 针对PSE1-PL human胎盘细胞
  • Ku70 抗体 (N3H10) 推荐用于WB, IP, IF 和 IHC(P) ，检测 mouse, rat, human 和 Xenopus来源的Ku70
  • 抗Ku70抗体（N3H10）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取Ku70 (N3H10): sc-56129的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP 和 m-IgG2b BP-HRP 是 Ku70 抗体 (N3H10) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与Ku70 抗体 (N3H10) 组合成套装提供。 (请参阅下面的订购信息)。

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描述
基因信息
说明书与实验方案
研究信息

関連項目

此外 Ku Lupus Antigen 抗体包括 Ku70 和 Ku86

Ku70抗体（N3H10）是一种小鼠单克隆IgG2b kappa轻链抗体，可通过蛋白质印迹（WB）、免疫沉淀（IP）、免疫荧光（IF）和免疫组织化学等方法检测小鼠、大鼠和人类样本中的Ku70。抗Ku70抗体（N3H10）有非结合型和多种结合型，包括琼脂糖、辣根过氧化物酶（HRP）、藻红蛋白（PE）、异硫氰酸荧光素（FITC）和多种Alexa Fluor®结合物。Ku70是Ku异二聚体的重要组成部分，与Ku86一起在细胞对DNA损伤的反应中发挥着至关重要的作用，特别是在非同源末端连接（NHEJ）修复途径中。该途径通过修复DNA双链断裂来维持基因组的稳定性，而DNA双链断裂可能由电离辐射和氧化应激等多种因素引起。Ku70与DNA末端结合有助于修复因子的募集，并保护DNA免受降解。Ku70还调节转录和细胞信号传导，在细胞过程中发挥着多方面的作用。Ku70 (N3H10)抗体使研究人员能够研究DNA修复机制及其在癌症等疾病中的影响，因为DNA修复途径在癌症中经常失调。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

订购信息

产品名称产品编号规格价格数量收藏夹

Ku70 抗体 (N3H10)

sc-56129
200 µg/ml
RMB2377.00

Ku70 (N3H10): m-IgG Fc BP-HRP 套装

sc-525649
200 µg Ab; 10 µg BP
RMB2662.00

Ku70 (N3H10): m-IgG2b BP-HRP 套装

sc-549605
200 µg Ab; 10 µg BP
RMB2662.00

Ku70 抗体 (N3H10) AC

sc-56129 AC
500 µg/ml, 25% agarose
RMB3129.00

Ku70 抗体 (N3H10) HRP

sc-56129 HRP
200 µg/ml
RMB2377.00

Ku70 抗体 (N3H10) FITC

sc-56129 FITC
200 µg/ml
RMB2482.00

Ku70 抗体 (N3H10) PE

sc-56129 PE
200 µg/ml
RMB2580.00

Ku70 抗体 (N3H10) Alexa Fluor® 488

sc-56129 AF488
200 µg/ml
RMB2685.00

Ku70 抗体 (N3H10) Alexa Fluor® 546

sc-56129 AF546
200 µg/ml
RMB2685.00

Ku70 抗体 (N3H10) Alexa Fluor® 594

sc-56129 AF594
200 µg/ml
RMB2685.00

Ku70 抗体 (N3H10) Alexa Fluor® 647

sc-56129 AF647
200 µg/ml
RMB2685.00

Ku70 抗体 (N3H10) Alexa Fluor® 680

sc-56129 AF680
200 µg/ml
RMB2685.00

Ku70 抗体 (N3H10) Alexa Fluor® 790

sc-56129 AF790
200 µg/ml
RMB2685.00

I would like to request a FREE 10 µg sample. How can I proceed? Thanks, Jacky

Asked by: Jacky01
Thank you for your question. Please contact Technical Service for more information on how to receive a 10 μg sample of our well characterized monoclonal antibody.
Answered by: Technical Support
Date published: 2018-12-05

I am using Ku-70 (N3H10): sc-56129 monoclonal antibody to stain paraffin embedded tissue. Do you recommend antigen retrieval when using this antibody?

Asked by: cjMara
We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-02-24
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Rated 5 out of 5 by from Better than PolyThis monoclonal antibody produced strong bands with no background. IHCP stain was nice and specific
Date published: 2017-01-26
Rated 5 out of 5 by from Good specificity shown by using transfectedGood specificity shown by using transfected and non-transfected 293T cells for over-expression of the human Ku-70 protein via Western Blot analysis. -SCBT QC
Date published: 2015-05-18
Rated 5 out of 5 by from Various publications support the use of thisVarious publications support the use of this antibody in WB using human embryonic kidney (HEK) 293T, normal human fibroblast strains (MRC-5 and WI-38), human osteosarcoma strain (U-2 OS), human lung adenocarcinoma cell line A549 and p53-deficient lung carcinoma H1299 cells. -SCBT Publication Review
Date published: 2015-04-25
Rated 5 out of 5 by from It has been reported in literature that thisIt has been reported in literature that this antibody is suitable for use in WB using Xenopus sperm. -SCBT Publication Review
Date published: 2015-03-24
Rated 5 out of 5 by from Publishable data obtained by IP and WB usingPublishable data obtained by IP and WB using human H358 and H322 NSCLC cell lines. -SCBT Publication Review
Date published: 2015-03-14
Rated 5 out of 5 by from Positive detection of endogenous expression of KuPositive detection of endogenous expression of Ku-70 protein by WB in K-562, HeLa and C32 cell lines. -SCBT QC
Date published: 2014-07-15
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Ku70 抗体 (N3H10) is rated 5.0 out of 5 by 6.
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