Date published: 2025-9-9
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Ku70 Antibody (A-9) - Western Blotting - Image 353986
Ku70 抗体 (A-9): sc-5309. 对 SK-MEL-24 (A) 和 MDA-MB-435S (F) 全细胞裂解液以及 A-431 (B), K-562 (C), Jurkat (D) 和 MCF7 (E) 核提取物中 Ku70 的表达进行 Western 印迹分析.
Ku70 抗体 (A-9): sc-5309. 对 SK-MEL-24 (A) 和 MDA-MB-435S (F) 全细胞裂解液以及 A-431 (B), K-562 (C), Jurkat (D) 和 MCF7 (E) 核提取物中 Ku70 的表达进行 Western 印迹分析.

Ku70 抗体 (A-9): sc-5309

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说明书
  • Ku70抗体(A-9)是小鼠单克隆IgG2a κ, 在78篇文献中引用，规格为200 µg/ml
  • 针对氨基酸的 302-609 Ku70 human 起源的 Ku 蛋白
  • Ku70 抗体 (A-9) 推荐用于WB, IP, IF, IHC(P), FCM 和 ELISA，检测human 来源的 Ku70
  • 抗Ku70抗体（A-9）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取Ku70 (A-9): sc-5309的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP, m-IgG2a BP-HRP 和 m-IgGκ BP-HRP 是 Ku70 抗体 (A-9) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与Ku70 抗体 (A-9) 组合成套装提供。 (请参阅下面的订购信息)。

快捷链接

相关产品
描述
基因信息
蛋白序列
说明书与实验方案
研究信息

関連項目

此外 Ku Lupus Antigen 抗体 包括 Ku86

Ku70抗体（A-9）是一种小鼠单克隆IgG2a kappa轻链抗体，可通过多种应用检测人类来源的Ku70蛋白，包括蛋白质印迹、免疫沉淀、免疫荧光、免疫组织化学、流式细胞术（FCM）和酶联免疫吸附测定。Ku70 (A-9) 抗体有非结合型和多种结合型，如琼脂糖、辣根过氧化物酶、藻红蛋白、异硫氰酸荧光素和多种 Alexa Fluor® 结合物。Ku70 蛋白位于细胞核中，在 DNA 修复和基因组稳定性维护中发挥着关键作用。Ku70与Ku86形成异二聚体复合物，对于识别和结合DNA双链断裂至关重要，从而促进非同源末端连接（NHEJ）修复途径。该功能对于细胞对DNA损伤的反应至关重要，因为Ku70有助于防止突变并保持基因组的完整性，这在癌症和其他遗传疾病中尤为重要。Ku70蛋白还参与转录调控，影响细胞周期控制和细胞凋亡相关基因的表达。在研究中使用Ku70 (A-9)单克隆抗体可以深入了解DNA修复机制和Ku70在各种疾病中的影响，使Ku70 (A-9)抗体成为研究细胞对DNA损伤反应的宝贵工具。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

Ku70 抗体 (A-9) 参考文献:

  1. 一种 DNA 结合型核自身抗原 Ku 的 70-kD 成分在细胞表面的表达。  |  Prabhakar, BS., et al. 1990. J Clin Invest. 86: 1301-5. PMID: 2212014
  2. 自身抗原 Ku 与形成多聚蛋白-DNA 复合物的 NF IV 蛋白没有区别。  |  Stuiver, MH., et al. 1990. J Exp Med. 172: 1049-54. PMID: 2212941
  3. 编码 p70 (Ku) 狼疮自身抗原 cDNA 的分子克隆。  |  Reeves, WH. and Sthoeger, ZM. 1989. J Biol Chem. 264: 5047-52. PMID: 2466842
  4. 从 cDNA 提取的 Ku 抗原 86-kDa 亚基氨基酸序列。  |  Yaneva, M., et al. 1989. J Biol Chem. 264: 13407-11. PMID: 2760028
  5. 克隆编码 70 kDa 新型人类甲状腺自身抗原的 cDNA 并确定其特征。  |  Chan, JY., et al. 1989. J Biol Chem. 264: 3651-4. PMID: 2917966
  6. CIRKIL通过与Ku70相互作用加剧心脏缺血/再灌注损伤。  |  Xiao, H., et al. 2022. Circ Res. 130: e3-e17. PMID: 35105170
  7. 风湿病患者自身抗体识别的 DNA 结合蛋白抗原 Ku 的特征。  |  Mimori, T., et al. 1986. J Biol Chem. 261: 2274-8. PMID: 3511059
  8. SETD4 介导的 KU70 甲基化抑制细胞凋亡。  |  Wang, Y., et al. 2022. Cell Rep. 39: 110794. PMID: 35545041
  9. Ku70与YAP结合改变PARP1泛素化以调节基因组稳定性和肿瘤发生  |  Shu, Y., et al. 2024. Cancer Res. 84: 2836-2855. PMID: 38862269
  10. 多发性肌炎-硬皮病重叠患者血清中自身抗体识别的高分子量酸性核蛋白的特征。  |  Mimori, T., et al. 1981. J Clin Invest. 68: 611-20. PMID: 7276162
  11. 增强子 1 结合因子是一种 Ku 相关蛋白，是 RNA 聚合酶 I 转录启动的正向调节因子。  |  Hoff, CM., et al. 1994. Proc Natl Acad Sci U S A. 91: 762-6. PMID: 8290597
  12. DNA 依赖性蛋白激酶：对 DNA 末端的要求以及与 Ku 抗原的关联。  |  Gottlieb, TM. and Jackson, SP. 1993. Cell. 72: 131-42. PMID: 8422676

订购信息

产品名称产品编号规格价格数量收藏夹

Ku70 抗体 (A-9)

sc-5309
200 µg/ml
RMB2377.00

Ku70 (A-9): m-IgG Fc BP-HRP 套装

sc-528160
200 µg Ab; 10 µg BP
RMB2662.00

Ku70 (A-9): m-IgGκ BP-HRP 套装

sc-520468
200 µg Ab, 40 µg BP
RMB2662.00

Ku70 (A-9): m-IgG2a BP-HRP 套装

sc-547035
200 µg Ab; 10 µg BP
RMB2662.00

Ku70 抗体 (A-9) AC

sc-5309 AC
500 µg/ml, 25% agarose
RMB3129.00

Ku70 抗体 (A-9) HRP

sc-5309 HRP
200 µg/ml
RMB2377.00

Ku70 抗体 (A-9) FITC

sc-5309 FITC
200 µg/ml
RMB2482.00

Ku70 抗体 (A-9) PE

sc-5309 PE
200 µg/ml
RMB2580.00

Ku70 抗体 (A-9) Alexa Fluor® 488

sc-5309 AF488
200 µg/ml
RMB2685.00

Ku70 抗体 (A-9) Alexa Fluor® 546

sc-5309 AF546
200 µg/ml
RMB2685.00

Ku70 抗体 (A-9) Alexa Fluor® 594

sc-5309 AF594
200 µg/ml
RMB2685.00

Ku70 抗体 (A-9) Alexa Fluor® 647

sc-5309 AF647
200 µg/ml
RMB2685.00

Ku70 抗体 (A-9) Alexa Fluor® 680

sc-5309 AF680
200 µg/ml
RMB2685.00

Ku70 抗体 (A-9) Alexa Fluor® 790

sc-5309 AF790
200 µg/ml
RMB2685.00

Can you recommend a positive control to use with Ku-70 (A-9): sc-5309 monoclonal antibody for Western blot?

Asked by: jenniferc
We recommend SK-MEL-24 (sc-364259) or MDA-MB-435S (sc-364184) whole cell lysates and A-431 (sc-2122), K-562 (sc-2130), Jurkat (sc-2132) or MCF7 (sc-2149) nuclear extracts to use as positive controls as we have Western blot data showing specific detection of Ku-70 in these cell lines.
Answered by: Technical Support 9
Date published: 2017-01-11

What secondary is best to use with Ku-70 (A-9): sc-5309 monoclonal antibody?

Asked by: jenniferc
Thank you for your question. While most anti-mouse IgG secondary antibodies are appropriate for use with Ku-70 (A-9): sc-5309, we recommend using one our exclusive Mouse IgG Binding Proteins as a secondary detection reagent. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Answered by: Technical Support 9
Date published: 2017-01-11
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Rated 5 out of 5 by from Very good antibody!The antibody was tested in WB and gave a very clean band at the expected molecular weight.
Date published: 2022-09-19
Rated 5 out of 5 by from GoodGood antibody for western blot (1:2000 dilution) in lung cancer cell lines
Date published: 2018-07-19
Rated 5 out of 5 by from Great Immunofluorescence stainingI tried a sample of this antibody on human fibroblasts and used it at a dilution of 1:200. The staining looks strong and specific. I think this antibody produces stronger staining than the Ku70 E-5 antibody.
Date published: 2017-12-05
Rated 5 out of 5 by from Works greatUsed for western blot at 1:2500 in HeLa and 293T. Highly specific!
Date published: 2017-09-06
Rated 5 out of 5 by from suitable for WBwestern blot of MCF-7 cell lysate with dilution of 1:4000
Date published: 2017-08-26
Rated 5 out of 5 by from Excellent for western blotThe antibody detects a single band of the right size from HuH7 cell lysates at a 1:8000X dilution. It may even work with a higher dilution ratio.
Date published: 2017-07-26
Rated 5 out of 5 by from Great western and IHCSuperb western blot data; strong signal, low background; also great IHC staining
Date published: 2017-01-28
Rated 5 out of 5 by from Great WB data!The Ku-70 Antibody (A-9): sc-5309 provides excellent Western blot data showing clear band with minimal background. This antibody has been cited over 30 times for further verification.
Date published: 2017-01-11
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Ku70 抗体 (A-9) is rated 4.9 out of 5 by 20.
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