Date published: 2025-9-8
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Ku70 Antibody (G-7) - Western Blotting - Image 377604
Ku70 抗体 (G-7): sc-55505. HeLa (A), K-562 (B), Hep G2 (C), HEL 92. 1. 7 (D) 和 Jurkat (E) 全细胞裂解液及 HeLa 核提取物 (F) 中 Ku70 表达的 Western 印迹分析.
Ku70 抗体 (G-7): sc-55505. HeLa (A), K-562 (B), Hep G2 (C), HEL 92. 1. 7 (D) 和 Jurkat (E) 全细胞裂解液及 HeLa 核提取物 (F) 中 Ku70 表达的 Western 印迹分析.

Ku70 抗体 (G-7): sc-55505

5.0(4)
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说明书
  • Ku70抗体(G-7)是小鼠单克隆IgG1 κ, 在3篇文献中引用，规格为200 µg/ml
  • 免疫human物种的Ku-70的氨基酸302-609
  • 推荐用于 human 来源的Ku70 WB, IP, IF, IHC(P) 和 ELISA检测
  • 看 Ku70 (E-5): sc-17789，可查看有标记的 Ku70 抗体，包括AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 和 790
  • m-IgG Fc BP-HRP, m-IgG1 BP-HRP 和 m-IgGκ BP-HRP 是 Ku70 抗体 (G-7) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与Ku70 抗体 (G-7) 组合成套装提供。 (请参阅下面的订购信息)。

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研究信息

関連項目

附加Ku Lupus Antigen抗体，包括Ku70和Ku86

Ku蛋白位于细胞核内，由被称为Ku70（p70）和Ku86（p86）的亚基组成，也被称为Ku80或（p80）。Ku最初被描述为一种自身抗原，在硬皮病多肌炎重叠综合征患者中产生抗体，后来在其他风湿性疾病患者的血清中也发现了这种抗体。Ku蛋白的两个亚基都已被克隆，并提出了Ku的许多功能，包括细胞信号传导、DNA复制和转录激活。Ku通过其DNA结合活性参与Pol II指导的转录，作为DNA相关蛋白激酶的调节成分，使Pol II和转录因子Sp磷酸化。Ku蛋白还从U1小核RNA和人转铁蛋白受体基因启动子激活转录。一种由两个亚基组成的Ku相关蛋白增强子1结合因子（E1BF）已被鉴定为RNA聚合酶I转录起始的正调节因子。

仅限研究使用。不适用于诊断和治疗用途。

订购信息

产品名称产品编号规格价格数量收藏夹

Ku70 抗体 (G-7)

sc-55505
200 µg/ml
RMB2377.00

Ku70 (G-7): m-IgG Fc BP-HRP 套装

sc-537057
200 µg Ab; 10 µg BP
RMB2662.00

Ku70 (G-7): m-IgGκ BP-HRP 套装

sc-534242
200 µg Ab; 40 µg BP
RMB2662.00

Ku70 (G-7): m-IgG1 BP-HRP 套装

sc-544979
200 µg Ab; 20 µg BP
RMB2662.00

Can Ku-70 (G-7): sc-55505 monoclonal antibody be used to stain formalin-fixed, paraffin-embedded (FFPE) tissue sections?

Asked by: Trav11
Thank you for your question. Yes, Ku-70 (G-7): sc-55505 has been validated for use in IHC with paraffin-embedded sections. We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-02-27
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Rated 5 out of 5 by from Positive detection of endogenously expressed KuPositive detection of endogenously expressed Ku-70 protein by WB using HeLa, A431 and 293T whole cell lysates. -SCBT QC
Date published: 2015-04-28
Rated 5 out of 5 by from Good specificity shown by using transfectedGood specificity shown by using transfected and non-transfected 293T cells for over-expression of the human Ku-70 protein via Western Blot analysis. -SCBT QC
Date published: 2015-04-16
Rated 5 out of 5 by from Produced great immunoperoxidase nuclear stainingProduced great immunoperoxidase nuclear staining in formalin fixed, paraffin-embedded human appendix tissue. -SCBT QC
Date published: 2015-01-29
Rated 5 out of 5 by from Nuclear localization of the KuNuclear localization of the Ku-70 protein demonstrated by immunofluorescence staining of methanol fixed HeLa cells. -SCBT QC
Date published: 2015-01-07
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Ku70 抗体 (G-7) is rated 5.0 out of 5 by 4.
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