Date published: 2025-9-8
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Ku70 Antibody (E-5) - Western Blotting - Image 377430
Ku70 抗体 (E-5): sc-17789. 在 Jurkat (A) 和 HL-60 (B) 核提取物以及 Daudi 全细胞裂解液 (C) 中 Ku70 表达的 Western 印迹分析.
Ku70 抗体 (E-5): sc-17789. 在 Jurkat (A) 和 HL-60 (B) 核提取物以及 Daudi 全细胞裂解液 (C) 中 Ku70 表达的 Western 印迹分析.

Ku70 抗体 (E-5): sc-17789

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寫評論提問

说明书
  • Ku70 抗体 E-5 是小鼠单克隆 IgG1 κ，Ku70抗体, 在93篇文献中引用，规格为200 µg/ml
  • 针对氨基酸302-609的抗原，在Ku70的C-terminus处与human来源的Ku蛋白进行比对
  • Ku70 抗体 (E-5) 推荐用于 WB, IP, IF, IHC(P) 和 ELISA，检测mouse, rat 和human 来源的 Ku70
  • 抗Ku70抗体（E-5）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取Ku70 (E-5): sc-17789的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP, m-IgG1 BP-HRP 和 m-IgGκ BP-HRP 是 Ku70 抗体 (E-5) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与Ku70 抗体 (E-5) 组合成套装提供。 (请参阅下面的订购信息)。

快捷链接

相关产品
描述
基因信息
蛋白序列
说明书与实验方案
研究信息

関連項目

此外 Ku Lupus Antigen 抗体 包括 Ku86

Ku70抗体（E-5）是一种鼠单克隆IgG1 kappa轻链抗体，可通过蛋白质印迹（WB）、免疫沉淀（IP）、免疫荧光（IF）、免疫组织化学和酶联免疫吸附测定（ELISA）检测小鼠、大鼠和人源Ku70蛋白。抗Ku70抗体（E-5）有非结合型和多种结合型，包括琼脂糖、辣根过氧化物酶（HRP）、藻红蛋白（PE）、异硫氰酸荧光素（FITC）和多种Alexa Fluor®结合物。Ku70是Ku异源二聚体的重要组成部分，与Ku86一起主要位于细胞核中，在DNA修复和基因组稳定性维护方面发挥着重要作用。Ku70对于非同源末端连接（NHEJ）途径至关重要，该途径可修复DNA双链断裂，这种常见的损伤形式如果得不到修复，会导致突变和癌症。Ku70能够与DNA末端结合并招募其他修复因子，这凸显了Ku70在细胞对DNA损伤的反应中的重要性，使Ku70成为维持基因组完整性的关键因素。Ku70与多种细胞过程有关，包括转录调控和细胞信号传导，这进一步凸显了Ku70在细胞稳态和应激反应中的多面作用。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

Ku70 抗体 (E-5) 参考文献:

  1. 利用 BioID2 鉴定 Ku70 结构域特异性相互作用者  |  Abbasi, S. and Schild-Poulter, C. 2021. Cells. 10: PMID: 33799447
  2. Ku70抑制G1期停滞祖细胞B细胞的替代末端连接。  |  Liang, Z., et al. 2021. Proc Natl Acad Sci U S A. 118: PMID: 34006647
  3. Ku70作为细胞质DNA传感器在先天免疫中的作用。  |  Sui, H., et al. 2021. Front Cell Infect Microbiol. 11: 761983. PMID: 34746031
  4. 综合分析揭示前列腺癌伊维菌素的靶点是 FOXA1 和 Ku70/Ku80。  |  Lv, S., et al. 2022. Cell Death Dis. 13: 754. PMID: 36050295
  5. 信号诱导的增强子激活需要Ku70读取拓扑异构酶1-DNA共价复合物。  |  Tan, Y., et al. 2023. Nat Struct Mol Biol. 30: 148-158. PMID: 36747093
  6. Parkin对Ku70的泛素化作用促进晶状体上皮细胞的凋亡。  |  Zhang, W., et al. 2023. FEBS J. 290: 3828-3842. PMID: 37000041
  7. UBE4B与ITCH E3泛素连接酶相互作用，诱导Ku70和c-FLIPL的多聚泛素化，并增强神经母细胞瘤的凋亡。  |  Le Clorennec, C., et al. 2023. Cell Death Dis. 14: 739. PMID: 37957138
  8. Ku70感知细胞质DNA并组装肿瘤抑制信号复合物。  |  Pandey, A., et al. 2024. Sci Adv. 10: eadh3409. PMID: 38277448
  9. Ku70的非经典作用通过FOXP3依赖的机制在肺腺癌中促进Treg抑制功能。  |  Huang, Q., et al. 2024. J Clin Invest. 134: PMID: 39446493
  10. 核刺猬蛋白在DNA损伤反应中介导XRCC6/Ku70的S-棕榈酰化。  |  Chen, Y., et al. 2024. Exp Hematol Oncol. 13: 109. PMID: 39497152

订购信息

产品名称产品编号规格价格数量收藏夹

Ku70 抗体 (E-5)

sc-17789
200 µg/ml
RMB2377.00

Ku70 (E-5): m-IgG Fc BP-HRP 套装

sc-528315
200 µg Ab; 10 µg BP
RMB2662.00

Ku70 (E-5): m-IgGκ BP-HRP 套装

sc-520659
200 µg Ab, 40 µg BP
RMB2662.00

Ku70 (E-5): m-IgG1 BP-HRP 套装

sc-542862
200 µg Ab; 20 µg BP
RMB2662.00

Ku70 抗体 (E-5) AC

sc-17789 AC
500 µg/ml, 25% agarose
RMB3129.00

Ku70 抗体 (E-5) HRP

sc-17789 HRP
200 µg/ml
RMB2377.00

Ku70 抗体 (E-5) FITC

sc-17789 FITC
200 µg/ml
RMB2482.00

Ku70 抗体 (E-5) PE

sc-17789 PE
200 µg/ml
RMB2580.00

Ku70 抗体 (E-5) Alexa Fluor® 488

sc-17789 AF488
200 µg/ml
RMB2685.00

Ku70 抗体 (E-5) Alexa Fluor® 546

sc-17789 AF546
200 µg/ml
RMB2685.00

Ku70 抗体 (E-5) Alexa Fluor® 594

sc-17789 AF594
200 µg/ml
RMB2685.00

Ku70 抗体 (E-5) Alexa Fluor® 647

sc-17789 AF647
200 µg/ml
RMB2685.00

Ku70 抗体 (E-5) Alexa Fluor® 680

sc-17789 AF680
200 µg/ml
RMB2685.00

Ku70 抗体 (E-5) Alexa Fluor® 790

sc-17789 AF790
200 µg/ml
RMB2685.00

Not sure if I am in the right place but can any of this be used to correct defective genes? An example would be TSC1 on chromosome 9, and TSC2 on chromosome 16. Thanks for a reply from anybody.

Asked by: momof4spoiled1s
Thank you for your question. It would be helpful if you could call 800-457-3801, allowing for a more interactive discussion of this and other related questions.
Answered by: Technical Service
Date published: 2024-03-28

Can this antibody be used for ChIP?

Asked by: zire
Thank you for your question. Ku70 Antibody (E-5) is recommended for detection of Ku70 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA. It has not been tested in ChIP applications.
Answered by: Tech Support Europe
Date published: 2023-02-28

Can this antibody be used for co-precipitation of Ku70 with other proteins rather than just immunoprecipitation? especially, the interaction between Ku70 and HDAC, or you can recommend a Ku70 that can do HDAC interaction.

Asked by: gongping1125
Thank you for your question. This antibody has not been tested for Co-IP, so we can not say for sure that it will work for that use. It is recommended and warrantied for standard IP use. A good choice for co-IP may be sc-5309 as it does have previous publications for Co-IP use.
Answered by: Tech Service
Date published: 2019-08-29

Can this Ku-70 mouse monoclonal antibody be used in IF or IHC with mouse tissue or cells? Will there be non-specific staining?

Asked by: cjMara
Thank you for your inquiry. The use of mouse monoclonal antibodies with mouse samples is very common and typically poses no problem. To eliminate any potential cross-reactivity of an anti-mouse secondary detection reagent, our Ku-70 (E-5): sc-17789 is available in a variety of direct conjugations, such as HRP, PE, FITC, AlexaFluor 488 and AlexaFluor 647.
Answered by: Technical Support 7
Date published: 2017-01-19
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Rated 5 out of 5 by from Great antibodyWorks very well by western blot (1:1000 dilution); I saw a clear band at the expected molecular weight
Date published: 2022-02-16
Rated 5 out of 5 by from works greatI used this anti-Ku70 both in WB and IFA, IFA dilute at 1:200, it works very well, there is no non-specific binding , great！
Date published: 2021-12-31
Rated 5 out of 5 by from Good antibody for westernGood specificity and signal strength. Induced upon dsDNA break formation
Date published: 2021-09-14
Rated 5 out of 5 by from Quite clear band for wb of rat protein1:200 diluted for wb of nuclear protein,the band is quite strong
Date published: 2018-05-15
Rated 5 out of 5 by from very strong signalVery robust signal [Western Blots] in mouse spleen tissue.
Date published: 2018-04-19
Rated 5 out of 5 by from Great Immunofluorescence stainingI tried a sample of this antibody on cultured human fibroblasts fixed in Histochoice. The staining was great! I used it at a dilution of 1:200.
Date published: 2017-12-05
Rated 5 out of 5 by from Works for WB/IF/PLAWorks great at 1:1000 for WB, 1:200 for IF. Incredibly pleased with this product!
Date published: 2017-08-30
Rated 5 out of 5 by from GreatThe antibody is pretty good for IF staining. The background is very low.
Date published: 2017-08-02
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Ku70 抗体 (E-5) is rated 4.7 out of 5 by 31.
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