Date published: 2025-9-7

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Kinectin 1 抗体 (A-12): sc-374577

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说明书
  • Kinectin 1 抗体 A-12 是小鼠单克隆 IgG3 (kappa轻链),Kinectin 1抗体,规格为200 µg/ml
  • 免疫原氨基酸序列1156-1345位于human物种的Kinectin 1的C-terminus附近
  • 推荐用于 human 来源的Kinectin 1 isoforms 1 and 2 WB, IP, IF, IHC(P) 和 ELISA检测
  • 关于如何获取Kinectin 1 (A-12): sc-374577免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG3 BP-HRP是Kinectin 1 Antibody (A-12) 适用于 WB 和 IHC(P) 应用。 的首选辅助检测试剂,该试剂现与Kinectin 1 Antibody (A-12) 搭配使用(请参阅下面的订购信息)。

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    Kinectin 1抗体(A-12)是一种IgG3 κ小鼠单克隆Kinectin 1抗体(也称为Kinectin 1抗体),通过蛋白质印迹法(WB)、免疫沉淀法(IP)、免疫荧光法(IF)、免疫组织化学法(IHC(P))和酶联免疫吸附测定法(ELISA)检测人源性Kinectin 1蛋白。Kinectin 1抗体(A-12)可作为非偶联抗Kinectin 1抗体使用。驱动蛋白家族的马达蛋白至少包含两种形式的传统驱动蛋白,它们由不同的基因编码,并被命名为普遍存在的驱动蛋白和神经元驱动蛋白。前者在所有细胞和组织中都有表达,而后者仅在神经细胞中表达。马达蛋白驱动蛋白是一个由两条重链和两条轻链组成的异四聚体。虽然驱动蛋白的重链具有运动活性,但有证据表明,驱动蛋白的轻链要么参与调节重链的活性,要么参与货物的结合。驱动蛋白驱动的囊泡运动依赖于驱动蛋白结合蛋白kinectin 1。Kinectin 1也被称为驱动蛋白受体,是内质网中的一种整体膜蛋白。尽管存在驱动蛋白与kinectin 1的相互作用,但关于kinectin 1是否参与微管依赖性运输仍存在大量争议。

    仅限研究使用。不适用于诊断和治疗用途。

    Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

    LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

    Kinectin 1 抗体 (A-12) 参考文献:

    1. 小鼠驱动蛋白轻链突变体中驱动蛋白重链的缺陷行为  |  Rahman, A., et al. 1999. J Cell Biol. 146: 1277-88. PMID: 10491391
    2. 在正常人的黑色素细胞中,驱动蛋白和激肽能与黑色素体表面缔合作用,并与微管形成联系。  |  Vancoillie, G., et al. 2000. J Invest Dermatol. 114: 421-9. PMID: 10692099
    3. KTN1 变异与酒精和药物依赖之间存在显著的、可复制的功能性关联。  |  Luo, X., et al. 2021. Addict Biol. 26: e12888. PMID: 32115811
    4. KTN1 变异与注意力缺陷多动障碍的风险。  |  Luo, X., et al. 2020. Am J Med Genet B Neuropsychiatr Genet. 183: 234-244. PMID: 32190980
    5. Rab18 通过与内质网上的 kinectin-1 相互作用来调节病灶粘附动力学。  |  Guadagno, NA., et al. 2020. J Cell Biol. 219: PMID: 32525992
    6. Kinectin 1 通过直接共同激活 NF-kappaB/p65 并增强其转录物活性来促进三阴性乳腺癌的生长。  |  Gao, L., et al. 2021. Signal Transduct Target Ther. 6: 250. PMID: 34219129
    7. 上调 KTN1 会促进肝细胞癌的发展。  |  Pan, J., et al. 2021. J Cancer. 12: 4791-4809. PMID: 34234850
    8. 一个重要的、功能性的和可复制的精神分裂症风险 KTN1 变异区块。  |  Mao, Q., et al. 2023. Sci Rep. 13: 3890. PMID: 36890161
    9. 驱动蛋白驱动囊泡运动的重要锚--Kinectin。  |  Kumar, J., et al. 1995. Science. 267: 1834-7. PMID: 7892610
    10. 大鼠中枢神经系统中神经元驱动蛋白重链的表达受发育调控  |  Vignali, G., et al. 1997. J Neurochem. 69: 1840-9. PMID: 9349526
    11. 小鼠的两种驱动蛋白轻链基因。编码蛋白的鉴定和特征描述。  |  Rahman, A., et al. 1998. J Biol Chem. 273: 15395-403. PMID: 9624122
    12. 无处不在的人类驱动蛋白重链的柄结构域的 C 端区域包含驱动蛋白轻链的结合位点。  |  Diefenbach, RJ., et al. 1998. Biochemistry. 37: 16663-70. PMID: 9843434

    订购信息

    产品名称产品编号规格价格数量收藏夹

    Kinectin 1 抗体 (A-12)

    sc-374577
    200 µg/ml
    RMB2377.00

    Kinectin 1 (A-12): m-IgG3 BP-HRP 套装

    sc-550579
    200 µg Ab; 40 µg BP
    RMB2662.00

    Can Kinectin 1 (A-12): sc-374577 monoclonal antibody be used to stain formalin-fixed, paraffin-embedded (FFPE) tissue sections?

    Asked by: Cweed
    Thank you for your question. Yes, Kinectin 1 (A-12): sc-374577 has been validated for use in IHC with paraffin-embedded sections. We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
    Answered by: Technical Support
    Date published: 2017-02-27
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    Rated 5 out of 5 by from good antibodyit worked really well at 1:400 in WB with a very low background
    Date published: 2018-11-29
    Rated 5 out of 5 by from Excellent antibody for immunoblotting!The SCBT Kinectin (sc-374577; A-12) antibody detects kinectin proteins at 150 and 250 kDa in HEK293 and HepG2 cell lysates respectively (see the attached Immunoblot). Researchers have reported 160kDa Kinectin protein in the ER fraction and 120kDa form in the mitochondria in mouse neuronal cells (see Santama et al 2004, PMID: 15316074). Also, note the unequal distribution of Kinectin isoforms in Hek293 and hepG2 cells. It’s an excellent antibody and gives very clean band. If you get lower/higher molecular band with this antibody then do not reject as nonspecific band, it might be something interesting (isoform or PTMs) and you must follow it. Who knows you may find something novel! Highly recommend this antibody for your research.
    Date published: 2018-03-02
    Rated 5 out of 5 by from Produced superb immunoperoxidase cytoplasmicProduced superb immunoperoxidase cytoplasmic staining in formalin fixed, paraffin-embedded human adrenal gland tissue. -SCBT QC
    Date published: 2015-05-18
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    Kinectin 1 抗体 (A-12) is rated 5.0 out of 5 by 4.
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