Date published: 2025-9-8
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JunD Antibody (D-9) - Western Blotting - Image 377621
Jun D 抗体 (D-9): sc-271938. SK-MEL-24 (A), Sol8 (B), C2C12 (C), A-10 (D) 和 PC-12 (E) 全细胞裂解液中 Jun D 表达的 Western 印迹分析.
Jun D 抗体 (D-9): sc-271938. SK-MEL-24 (A), Sol8 (B), C2C12 (C), A-10 (D) 和 PC-12 (E) 全细胞裂解液中 Jun D 表达的 Western 印迹分析.

JunD 抗体 (D-9): sc-271938

4.8(5)
寫評論提問

说明书
  • JunD 抗体 D-9 是小鼠单克隆 IgG1 κ，JunD抗体, 在16篇文献中引用，规格为200 µg/ml
  • 特异性抗原位于mouse物种的Jun D的C-terminus的氨基酸316-341之间
  • JunD 抗体 (D-9) 推荐用于 WB, IP, IF, IHC(P) 和 ELISA，检测mouse, rat 和human 来源的 Jun D; 也和以下物种反应，包括： bovine
  • 抗JunD抗体（D-9）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 可用于凝胶迁移和ChIP的TransCruz试剂(sc-271938 X, 200 µg/0.1 ml)
  • 关于如何获取JunD (D-9): sc-271938的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • 目前，我们还没有完成JunD 抗体 (D-9)的首选二抗检测试剂的鉴定。这项工作正在进行中。

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描述
基因信息
说明书与实验方案
研究信息

関連項目

此外 Jun 抗体 包括 c-Jun, Jun B和Jun D

Jun D抗体（D-9）是一种IgG1 κ小鼠单克隆Jun D抗体（也称为JUND抗体），可通过WB、IP、IF、IHC(P)和ELISA检测小鼠、大鼠和人类来源的Jun D蛋白。Jun D抗体（D-9）既有非偶联抗Jun D抗体形式，也有多种偶联形式的抗Jun D抗体，包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®偶联物。激活蛋白-1（AP-1）转录因子由Jun/Jun同二聚体或Fos/Jun异二聚体复合物组成。同二聚体和异二聚体结合于存在于众多启动子中的TGACTCA共识序列，并最初被鉴定为佛波酯肿瘤启动子反应元件（TRE）。Jun B和Jun D在其C末端区域中与c-Jun几乎相同，都涉及二聚化和DNA结合，而它们的N末端结构域则涉及转录激活，有所不同。这三种都与c-Fos和Fos基因家族的其他成员形成异二聚体。研究表明，Jun D的两种形式可能是由于翻译的内部起始造成的。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

JunD 抗体 (D-9) 参考文献:

  1. JunD 保护细胞免于 p53 依赖性衰老和凋亡。  |  Weitzman, JB., et al. 2000. Mol Cell. 6: 1109-19. PMID: 11106750
  2. 迷走神经和颈交感干横断后大鼠神经系统中 c-JUN、JUN B 和 JUN D 蛋白的表达  |  Herdegen, T., et al. 1991. Neuroscience. 45: 413-22. PMID: 1762686
  3. 人外周血粒细胞中 c-JUN、JUN B 和 jun D 原癌基因的表达  |  Mollinedo, F., et al. 1991. Biochem J. 273(Pt 2): 477-9. PMID: 1899335
  4. 过表达 c-jun, junB 或 junD 对细胞生长的影响各不相同。  |  Castellazzi, M., et al. 1991. Proc Natl Acad Sci U S A. 88: 8890-4. PMID: 1924349
  5. JUN：癌基因和转录因子。  |  Vogt, PK. and Bos, TJ. 1990. Adv Cancer Res. 55: 1-35. PMID: 2166997
  6. jun-D：jun 基因家族的第三个成员。  |  Ryder, K., et al. 1989. Proc Natl Acad Sci U S A. 86: 1500-3. PMID: 2493644
  7. fos 相关基因的产物 fra-1 与 Jun 共同结合到 AP-1 位点：转录因子 AP-1 由多种蛋白质复合物组成。  |  Cohen, DR., et al. 1989. Genes Dev. 3: 173-84. PMID: 2497053
  8. junD的特征：jun原癌基因家族的新成员。  |  Hirai, SI., et al. 1989. EMBO J. 8: 1433-9. PMID: 2504580
  9. Fos 和 Jun：AP-1 的联系。  |  Curran, T. and Franza, BR. 1988. Cell. 55: 395-7. PMID: 3141060
  10. 血清生长因子诱导原癌基因 c-jun  |  Ryder, K. and Nathans, D. 1988. Proc Natl Acad Sci U S A. 85: 8464-7. PMID: 3186736
  11. 抗雌激素他莫昔芬诱导大鼠子宫中的c-fos和jun-B，但不诱导c-jun或jun-D原癌基因。  |  Nephew, KP., et al. 1993. Endocrinology. 133: 419-22. PMID: 8319588
  12. 循环, 静止和受刺激成纤维细胞中 Jun 和 Fos 蛋白表达及 AP-1 活性的变化。  |  Lallemand, D., et al. 1997. Oncogene. 14: 819-30. PMID: 9047389

订购信息

产品名称产品编号规格价格数量收藏夹

JunD 抗体 (D-9)

sc-271938
200 µg/ml
RMB2377.00

JunD 抗体 (D-9) X

sc-271938 X
200 µg/0.1 ml
RMB2377.00

JunD 抗体 (D-9) AC

sc-271938 AC
500 µg/ml, 25% agarose
RMB3129.00

JunD 抗体 (D-9) HRP

sc-271938 HRP
200 µg/ml
RMB2377.00

JunD 抗体 (D-9) FITC

sc-271938 FITC
200 µg/ml
RMB2482.00

JunD 抗体 (D-9) PE

sc-271938 PE
200 µg/ml
RMB2580.00

JunD 抗体 (D-9) Alexa Fluor® 488

sc-271938 AF488
200 µg/ml
RMB2685.00

JunD 抗体 (D-9) Alexa Fluor® 546

sc-271938 AF546
200 µg/ml
RMB2685.00

JunD 抗体 (D-9) Alexa Fluor® 594

sc-271938 AF594
200 µg/ml
RMB2685.00

JunD 抗体 (D-9) Alexa Fluor® 647

sc-271938 AF647
200 µg/ml
RMB2685.00

JunD 抗体 (D-9) Alexa Fluor® 680

sc-271938 AF680
200 µg/ml
RMB2685.00

JunD 抗体 (D-9) Alexa Fluor® 790

sc-271938 AF790
200 µg/ml
RMB2685.00

JunD (D-9) 中和勝肽

sc-271938 P
100 µg/0.5 ml
RMB511.00

In what dilution was this antibody used for IHC?

Asked by: Revital
Thank you for your question. We recommend a dilution range between 1:50 and 1:500 for IHC with this antibody. The ideal concentration is strongly dependent on your particular sample and experimental setup.
Answered by: Technical Support Europe
Date published: 2020-10-08

I have been using a rabbit polyclonal antibody against JunD (sc-74). I want to try this D-9 mouse monoclonal antibody to replace sc-74. Can you send me sample to try? Thank you very much.

Asked by: Tetsuaki Miyake
Thank you for your interest in our JunD antibody sc-271938. We do have a sample aliquot available of this antibody. To receive this sample or samples to other targets of interest, please contact Technical Service by phone, (800)-457-3801 option 2, email <[email protected]>, or by live chat directly on our website, www.scbt.com.
Answered by: Technical Service
Date published: 2017-04-19

What application is the blocking peptide sc-271938 P appropriate for?

Asked by: Professor Griffin
Thank you for your question. The blocking peptide is intended for use as a negative control, by pre-adsorbing the mouse monoclonal antibody against the antigen. For full protocol details, please contact our Technical Services Department or view our online protocol here: https://www.scbt.com/scbt/resources/protocols/peptide-neutralization
Answered by: Technical Support
Date published: 2017-02-24
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Rated 4 out of 5 by from GoodThe antibody in specific for JunD, tested for WV in MEFs and 293T
Date published: 2018-02-22
Rated 5 out of 5 by from Excellent specificityI checked this ab by comparison to very established rabbit polyclonal counterpart by Western blotting analysis. They detected endogenous JunD, and delta JunD in cell Lysander from C2C12 and HEK293T. There is no non specific reactivity @1:2500 dilution. Ectopically expressed JunD was detected as well.
Date published: 2017-05-16
Rated 5 out of 5 by from Works great for Western in PMA treated JurkatWorks great for Western in PMA treated Jurkat and PMA treated RAW 264.7 nuclear extracts. -SCBT QC
Date published: 2015-05-09
Rated 5 out of 5 by from Produced great immunofluorescence nuclearProduced great immunofluorescence nuclear staining in methanol-fixed HeLa cells. -SCBT QC
Date published: 2015-02-13
Rated 5 out of 5 by from Publication in WB and ChIP with human DU145Publication in WB and ChIP with human DU145. -SCBT QC
Date published: 2015-01-25
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JunD 抗体 (D-9) is rated 4.8 out of 5 by 5.
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