Date published: 2025-9-8
访问圣克鲁斯动物健康

021-6093-6350

SCBT Portrait Logo
Seach Input
联系我们
登陆账户
快速订购
购物车 (0)
JunB Antibody (C-11) - Western Blotting - Image 377618
Jun B 抗体 (C-11): sc-8051. U-251-MG (A), TK-1 (B) 和 A-10 (C) 全细胞裂解液及 NIH/3T3 核提取物 (D) 中 Jun B 表达的 Western 印迹分析.
Jun B 抗体 (C-11): sc-8051. U-251-MG (A), TK-1 (B) 和 A-10 (C) 全细胞裂解液及 NIH/3T3 核提取物 (D) 中 Jun B 表达的 Western 印迹分析.

JunB 抗体 (C-11): sc-8051

4.3(10)
寫評論提問

说明书
  • JunB 抗体 C-11 是小鼠单克隆 IgG1 κ，JunB抗体, 在160篇文献中引用，规格为200 µg/ml
  • 特异性抗原位于mouse物种的Jun B的C-terminus附近的氨基酸210-222之间
  • JunB 抗体 (C-11) 推荐用于 WB, IP, IF, IHC(P) 和 ELISA，检测mouse, rat 和human 来源的 Jun B
  • 抗JunB抗体（C-11）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 还可偶联TRITC 用于IF, IHC(P) 和FCM
  • 可用于凝胶迁移和ChIP的TransCruz试剂(sc-8051 X, 200 µg/0.1 ml)
  • 关于如何获取JunB (C-11): sc-8051的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP, m-IgG1 BP-HRP 和 m-IgGκ BP-HRP 是 JunB 抗体 (C-11) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与JunB 抗体 (C-11) 组合成套装提供。 (请参阅下面的订购信息)。

快捷链接

相关产品
描述
基因信息
说明书与实验方案
研究信息

関連項目

此外 Jun 抗体 包括 c-Jun, Jun B和Jun D

Jun B 抗体（C-11）是一种小鼠单克隆 IgG1 kappa 轻链抗体，可通过免疫印迹（WB）、免疫沉淀（IP）、免疫荧光（IF）、免疫组织化学（IHC）和酶联免疫吸附测定（ELISA）等方法检测小鼠、大鼠和人类的 Jun B。Jun B (C-11)抗体有非结合型和多种结合型，包括琼脂糖、辣根过氧化物酶、藻红蛋白、异硫氰酸荧光素和多种Alexa Fluor®结合物。Jun B在调节基因表达方面起着至关重要的作用，是AP-1转录复合物的重要组成部分，而AP-1转录复合物对于细胞增殖、分化、凋亡等多种细胞过程至关重要。Jun B可与c-Fos和Fos基因家族的其他成员形成同源二聚体或异源二聚体，从而与特定的DNA序列结合并调节靶基因转录。Jun B与这些蛋白的相互作用以及对转录的调节对于细胞对生长因子和应激信号的反应至关重要，因此Jun B在致癌过程和细胞信号通路中扮演着重要角色。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

JunB 抗体 (C-11) 参考文献:

  1. 巴西多发性骨髓瘤患者的N-RAS和K-RAS基因突变。  |  Ortega, MM., et al. 2006. Leuk Lymphoma. 47: 285-9. PMID: 16321859
  2. 过表达 c-jun, junB 或 junD 对细胞生长的影响各不相同。  |  Castellazzi, M., et al. 1991. Proc Natl Acad Sci U S A. 88: 8890-4. PMID: 1924349
  3. JUN：癌基因和转录因子。  |  Vogt, PK. and Bos, TJ. 1990. Adv Cancer Res. 55: 1-35. PMID: 2166997
  4. 小 GTPase N-Ras 可调节成纤维细胞细胞外基质的合成, 增殖和迁移。  |  Fuentes-Calvo, I., et al. 2013. Biochim Biophys Acta. 1833: 2734-2744. PMID: 23871832
  5. jun-D：jun 基因家族的第三个成员。  |  Ryder, K., et al. 1989. Proc Natl Acad Sci U S A. 86: 1500-3. PMID: 2493644
  6. fos 相关基因的产物 fra-1 与 Jun 共同结合到 AP-1 位点：转录因子 AP-1 由多种蛋白质复合物组成。  |  Cohen, DR., et al. 1989. Genes Dev. 3: 173-84. PMID: 2497053
  7. junD的特征：jun原癌基因家族的新成员。  |  Hirai, SI., et al. 1989. EMBO J. 8: 1433-9. PMID: 2504580
  8. 禽类细胞中癌基因 jun 的同源物。  |  Nishimura, T. and Vogt, PK. 1988. Oncogene. 3: 659-63. PMID: 2577867
  9. 禽肉瘤病毒 17 携带癌基因 jun。  |  Maki, Y., et al. 1987. Proc Natl Acad Sci U S A. 84: 2848-52. PMID: 3033666
  10. Fos 和 Jun：AP-1 的联系。  |  Curran, T. and Franza, BR. 1988. Cell. 55: 395-7. PMID: 3141060
  11. 血清生长因子诱导原癌基因 c-jun  |  Ryder, K. and Nathans, D. 1988. Proc Natl Acad Sci U S A. 85: 8464-7. PMID: 3186736
  12. 甲状腺肿瘤的N-ras突变，特别涉及滤泡型。  |  Oyama, T., et al. 1995. Pathol Int. 45: 45-50. PMID: 7704243

订购信息

产品名称产品编号规格价格数量收藏夹

JunB 抗体 (C-11)

sc-8051
200 µg/ml
RMB2377.00

JunB (C-11): m-IgG Fc BP-HRP 套装

sc-528216
200 µg Ab; 10 µg BP
RMB2662.00

JunB (C-11): m-IgGκ BP-HRP 套装

sc-520539
200 µg Ab, 40 µg BP
RMB2662.00

JunB (C-11): m-IgG1 BP-HRP 套装

sc-542821
200 µg Ab; 20 µg BP
RMB2662.00

JunB 抗体 (C-11) X

sc-8051 X
200 µg/0.1 ml
RMB2377.00

JunB 抗体 (C-11) AC

sc-8051 AC
500 µg/ml, 25% agarose
RMB3129.00

JunB 抗体 (C-11) HRP

sc-8051 HRP
200 µg/ml
RMB2377.00

JunB 抗体 (C-11) FITC

sc-8051 FITC
200 µg/ml
RMB2482.00

JunB 抗体 (C-11) PE

sc-8051 PE
200 µg/ml
RMB2580.00

JunB 抗体 (C-11) Alexa Fluor® 488

sc-8051 AF488
200 µg/ml
RMB2685.00

JunB 抗体 (C-11) Alexa Fluor® 546

sc-8051 AF546
200 µg/ml
RMB2685.00

JunB 抗体 (C-11) Alexa Fluor® 594

sc-8051 AF594
200 µg/ml
RMB2685.00

JunB 抗体 (C-11) Alexa Fluor® 647

sc-8051 AF647
200 µg/ml
RMB2685.00

JunB 抗体 (C-11) Alexa Fluor® 680

sc-8051 AF680
200 µg/ml
RMB2685.00

JunB 抗体 (C-11) Alexa Fluor® 790

sc-8051 AF790
200 µg/ml
RMB2685.00

JunB 抗体 (C-11) TRITC

sc-8051 TRITC
200 µg/ml
RMB2407.00

JunB (C-11) 中和勝肽

sc-8051 P
100 µg/0.5 ml
RMB511.00

Can you recommend a negative control to use with Jun B Antibody (C-11): sc-8051?

Asked by: jenni
We recommend using the blocking peptide sc-8051 P as a negative control. Antibody binding to antigen may be blocked/competed by pre-absorption with the blocking peptide. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/peptide-neutralization
Answered by: Technical Support 4
Date published: 2017-01-09
  • y_2025, m_9, d_7, h_4CST
  • bvseo_bulk, prod_bvqa, vn_bulk_3.0.42
  • cp_1, bvpage1
  • co_hasquestionsanswers, tq_1
  • loc_zh_CN, sid_8051, prod, sort_[SortEntry(order=LAST_APPROVED_ANSWER_SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-4.0.0
  • CLOUD, getContent, 148ms
  • QUESTIONS, PRODUCT
Rated 3 out of 5 by from SDS-PAGEIt worked OK, BUT it did not significantly better than previous one
Date published: 2018-03-30
Rated 4 out of 5 by from Worked goodI used on Western Blot for NIH3T3 cells with 1:200 dilution,worked good.
Date published: 2017-12-27
Rated 4 out of 5 by from Must use Santa Cruz secondary AbWe used this antibody several years ago. We requested a new sample and tested on HEK-293 cell lysates using a 1:500 dilution. We used Jackson Immuno Research anti-mouse IgG AF-680 secondary and imaged on the Licor Odyssey imager. No signal was detected. We requested a new sample along with the Santa Cruz secondary Ab (AF-680) that is specific for m-IgG Kappa Light Chain. It showed a nice band just under the 37 kD marker. Generally we have found that although whole IgG abs should still recognize these new mouse monoclonals, we get much better signal with the m-IgG Kappa Light Chain secondaries for most sample abs we have tested in both IHC and WB.
Date published: 2017-10-12
Rated 2 out of 5 by from cytoplasm stainingProduced immunofluorescence cytoplasm staining in methanol-fixed HK2 cells. why?
Date published: 2017-08-18
Rated 5 out of 5 by from Outstanding productStrong product for multiple applications; best available jun b antibody
Date published: 2017-01-10
Rated 5 out of 5 by from Good Antibody for IHCVersatile antibody showing great staining in human tissue samples as well as strong signal in WB and ELISA assays. Usable in human, mouse, and rat samples for a variety of applications.
Date published: 2016-12-20
Rated 5 out of 5 by from Produced beautiful immunofluorescence nuclearProduced beautiful immunofluorescence nuclear staining in methanol-fixed KNRK cells. -SCBT QC
Date published: 2015-05-01
Rated 5 out of 5 by from Worked great in our blotWorked great in our blot. We tested human
Date published: 2015-04-09
  • y_2025, m_9, d_7, h_4
  • bvseo_bulk, prod_bvrr, vn_bulk_3.0.42
  • cp_1, bvpage1
  • co_hasreviews, tv_0, tr_10
  • loc_zh_CN, sid_8051, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-4.0.0
  • CLOUD, getReviews, 18ms
  • REVIEWS, PRODUCT
JunB 抗体 (C-11) is rated 4.3 out of 5 by 10.
  • y_2025, m_9, d_7, h_4
  • bvseo_bulk, prod_bvrr, vn_bulk_3.0.42
  • cp_1, bvpage1
  • co_hasreviews, tv_0, tr_10
  • loc_zh_CN, sid_8051, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-4.0.0
  • CLOUD, getAggregateRating, 102ms
  • REVIEWS, PRODUCT
021-6093-6350
访问 Santa Cruz Animal Health|访问 San Juan Ranch
Santa Cruz Biotechnology, Inc. 是生物医学研究市场产品开发的世界领导者。请拨打0086-021-60936350联系我们。
版权所有© 2007-2025 Santa Cruz Biotechnology, Inc. 保留所有权利。”Santa Cruz Biotechnology” 和Santa Cruz Biotechnology, Inc.的商标，”Santa Cruz Animal Health”, “San Juan Ranch”, “Supplement of Champions”, San Juan Ranch的商标，”UltraCruz”, “ChemCruz”, “ImmunoCruz”, “ExactaCruz”, 和 “EZ Touch”是Santa Cruz Biotechnology, Inc.的注册商标。所有商标均为其各自所有者的财产。