Date published: 2026-10-24

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JAK1 Antibody (B-3) - Western Blotting - Image 362255
JAK1 抗体 (B-3): sc-376996. Jurkat (A), T-47D (B) 和 HEK293 (C) 全细胞裂解液中 JAK1 表达的 Western 印迹分析.
JAK1 抗体 (B-3): sc-376996. Jurkat (A), T-47D (B) 和 HEK293 (C) 全细胞裂解液中 JAK1 表达的 Western 印迹分析.

JAK1 抗体 (B-3): sc-376996

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寫評論提問
说明书
  • JAK1 抗体 B-3 是小鼠单克隆 IgG1 κ，JAK1抗体, 在24篇文献中引用，规格为200 µg/ml
  • 免疫原氨基酸序列270-375位于human物种的JAK1的N-terminus
  • JAK1 抗体 (B-3) 推荐用于WB, IP, IF 和 ELISA，检测human 来源的 JAK1
  • 抗JAK1抗体（B-3）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • m-IgG Fc BP-HRP、 1 BP-HRP">m-IgG1 BP-HRP和m-IgGκ BP-HRP是JAK1 Antibody (B-3) 适用于 WB 应用。 的首选辅助检测试剂。这些试剂现与JAK1 Antibody (B-3) 打包提供（请参阅下面的订购信息）。
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额外JAK抗体，包括JAK1, JAK2和JAK3

JAK1抗体（B-3）是一种IgG1 κ小鼠单克隆JAK1抗体（也被称为Janus激酶1抗体），能够通过WB、IP、IF和ELISA检测人类来源的JAK1蛋白。JAK1抗体（B-3）以非偶联的抗JAK1抗体形式提供，同时也以多种偶联形式的抗JAK1抗体提供，包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®偶联物。JAK1（Janus激酶1）属于非受体Janus酪氨酸激酶家族，在淋巴造血系统中调节活化细胞因子受体下游的一系列细胞功能。免疫学刺激，如干扰素和细胞因子，会诱导Stat转录因子招募到与细胞因子受体相关的JAK1。然后，JAK1使近端Stat因子磷酸化，这些因子随后二聚化，转位到细胞核并结合到目标基因启动子上游的顺式元件以调节转录。配体结合后，JAK1以相互依赖的方式发生酪氨酸磷酸化和催化激活。位于1022和1023位的酪氨酸残基的磷酸化被认为在催化事件的激活中起作用。典型的JAK-Stat途径通过刺激增殖、分化、存活和对病原体的宿主抗性，是维持正常免疫系统所必需的。改变JAK-Stat信号以减少细胞因子诱导的促炎反应代表了抗炎治疗的一个有吸引力的目标。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

JAK1 抗体 (B-3) 参考文献:

  1. 芦荟素通过抑制 RAW264.7 细胞中的 JAK1-STAT1/3 激活和 ROS 生成来抑制脂多糖诱导的炎症。  |  Ma, Y., et al. 2018. Int J Mol Med. 42: 1925-1934. PMID: 30066904
  2. 靶向 IFNGR/IL6R 或下游 JAK1/JAK2 以控制 GvHD。  |  Ashami, K., et al. 2018. Oncotarget. 9: 35721-35722. PMID: 30515263
  3. Filgotinib，一种JAK1抑制剂，用于治疗难治性类风湿性关节炎。  |  Singh, JA. 2019. JAMA. 322: 309-311. PMID: 31334773
  4. 使用JAK1/2抑制剂可改善因干扰素过度表达而导致的鼠自身免疫性胆管炎。  |  Shao, T., et al. 2022. Cell Mol Immunol. 19: 1130-1140. PMID: 36042351
  5. 巨噬细胞新基因缺乏会通过JAK1-STAT1信号传导加剧急性心肌梗死后心肌重塑和炎症。  |  Zhang, J., et al. 2023. Cell Mol Life Sci. 80: 324. PMID: 37824022
  6. JAK1 促进 HDV 复制，是抗病毒治疗的潜在靶标。  |  Heuschkel, MJ., et al. 2024. J Hepatol. 80: 220-231. PMID: 37925078
  7. 合成双JAK1/TYK2抑制剂在炎症性肠病中的治疗潜力。  |  Cui, X., et al. 2024. Int Immunopharmacol. 126: 111238. PMID: 37988912
  8. 多物种靶向siRNA调节皮肤中的JAK1。  |  Tang, Q., et al. 2024. Mol Ther Nucleic Acids. 35: 102117. PMID: 38304729
  9. JAK1变异个体受包括自身免疫、特应性、结肠炎和皮炎在内的综合征特征影响。  |  Horesh, ME., et al. 2024. J Exp Med. 221: PMID: 38563820
  10. 联合靶向JAK1/STAT6/GAS6/TAM信号通路可提高Ewing肉瘤的化疗效果。  |  Yu, L., et al. 2024. Nat Commun. 15: 5292. PMID: 38906855

订购信息

产品名称产品编号规格价格数量收藏夹

JAK1 抗体 (B-3)

sc-376996
200 µg/ml
CNY2422.00

JAK1 (B-3): m-IgG Fc BP-HRP 套装

sc-529953
200 µg Ab; 10 µg BP
CNY2715.00

JAK1 (B-3): m-IgGκ BP-HRP 套装

sc-523144
200 µg Ab, 40 µg BP
CNY2715.00

JAK1 (B-3): m-IgG1 BP-HRP 套装

sc-543783
200 µg Ab; 20 µg BP
CNY2715.00

JAK1 抗体 (B-3) AC

sc-376996 AC
500 µg/ml, 25% agarose
CNY3189.00

JAK1 抗体 (B-3) HRP

sc-376996 HRP
200 µg/ml
CNY2422.00

JAK1 抗体 (B-3) FITC

sc-376996 FITC
200 µg/ml
CNY2527.00

JAK1 抗体 (B-3) PE

sc-376996 PE
200 µg/ml
CNY2625.00

JAK1 抗体 (B-3) Alexa Fluor® 488

sc-376996 AF488
200 µg/ml
CNY2738.00

JAK1 抗体 (B-3) Alexa Fluor® 546

sc-376996 AF546
200 µg/ml
CNY2738.00

JAK1 抗体 (B-3) Alexa Fluor® 594

sc-376996 AF594
200 µg/ml
CNY2738.00

JAK1 抗体 (B-3) Alexa Fluor® 647

sc-376996 AF647
200 µg/ml
CNY2738.00

JAK1 抗体 (B-3) Alexa Fluor® 680

sc-376996 AF680
200 µg/ml
CNY2738.00

JAK1 抗体 (B-3) Alexa Fluor® 790

sc-376996 AF790
200 µg/ml
CNY2738.00

Can JAK1 (B-3): sc-376996 monoclonal antibody be used in IF or IHC with mouse tissue or cells? Will there be non-specific staining?

Asked by: Cweed
Thank you for your inquiry. The use of mouse monoclonal antibodies with mouse samples is very common and typically poses no problem. To eliminate any potential cross-reactivity of an anti-mouse secondary detection reagent, JAK1 (B-3): sc-376996 is available in a variety of direct conjugations, such as HRP, PE, FITC and AlexaFluors.
Answered by: Technical Support
Date published: 2017-02-24
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Rated 1 out of 5 by hannao from JAK1(B-3) antibody in rat cardiomyocytesvery weak signal in nuclei of cultures rat cardiomyocytes,
Date published: 2017-12-12
Rated 5 out of 5 by wally from goodLarge bands observed by Western Blot using Jurkat and MOLT-4 whole cell lysates. High background and some non-specific bands observed. -SCBT QC
Date published: 2017-09-07
Rated 5 out of 5 by dchan from Excellent antibody for westernsprobed at 1/500X dilution for 90min and used in conjunction with the Kappa-binding-HRP as secondary and the blot turned out perfect. Excellent antibody for western blotting.
Date published: 2017-06-23
Rated 5 out of 5 by Yoki from excellent in WB!the four lines in the right is the sample transfected JAK1 vector in human HeLa cells and the left lines is the Endogenous JAK1 . the figure is detected just several seconds after WB autoradiography. so the antibody is so good in wb.
Date published: 2017-04-26
Rated 5 out of 5 by TiffanyTan from Good for IP western blotI used this antibody for my IP WB on human samples, and it gave me strong band
Date published: 2017-03-30
Rated 5 out of 5 by LF from Produced superb immunofluorescence cytoplasmicProduced superb immunofluorescence cytoplasmic staining in methanol-fixed HeLa cells. -SCBT QC
Date published: 2015-05-03
Rated 4 out of 5 by QZ from Large bands observed by Western Blot usingLarge bands observed by Western Blot using Jurkat and MOLT-4 whole cell lysates. High background and some non-specific bands observed. -SCBT QC
Date published: 2015-02-02
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JAK1 抗体 (B-3) is rated 4.3 out of 5 by 7.
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