Date published: 2025-9-6
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IRF-7 Antibody (F-1) - Western Blotting - Image 375104
IRF-7 抗体 (F-1): sc-74471. 对 Raji (A) 和 THP-1 (B) 全细胞裂解液中 IRF-7 表达的 Western 印迹分析.
IRF-7 Antibody (F-1) - Western Blotting - Image 375104
IRF-7 抗体 (F-1): sc-74471. 对 Raji (A) 和 THP-1 (B) 全细胞裂解液中 IRF-7 表达的 Western 印迹分析.
IRF-7 Antibody (F-1) - Western Blotting - Image 17030
IRF-7 抗体 (F-1): sc-74471. Jurkat (A), PMA 处理的 Jurkat (B) 和 Raji (C) 全细胞裂解液中 IRF-7 表达的 Western 印迹分析.
IRF-7 Antibody (F-1) - Western Blotting - Image 377062
IRF-7 抗体 (F-1): sc-74471. GA-10 全细胞裂解液中 IRF-7 表达的 Western 印迹分析.
IRF-7 抗体 (F-1): sc-74471. 对 Raji (A) 和 THP-1 (B) 全细胞裂解液中 IRF-7 表达的 Western 印迹分析.

IRF-7 抗体 (F-1): sc-74471

4.4(8)
寫評論提問

说明书
  • IRF-7抗体(F-1)是小鼠单克隆IgG2a κ, 在22篇文献中引用，规格为200 µg/ml
  • 免疫human物种的IRF-7的氨基酸1-246
  • 抗-IRF-7 抗体 (F-1) 推荐用于 WB, IP, IF 和 ELISA，检测mouse, rat 和human 来源的 IRF-7
  • 抗IRF-7抗体（F-1）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 可用于凝胶迁移和ChIP的TransCruz试剂(sc-74471 X, 200 µg/0.1 ml)
  • 关于如何获取IRF-7 (F-1): sc-74471的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP和 2a BP-HRP">m-IgG2a BP-HRP是IRF-7 Antibody (F-1) 适用于 WB 应用。 的首选辅助检测试剂。这些试剂现与IRF-7 Antibody (F-1) 打包提供（请参阅下面的订购信息）。

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相关产品
描述
基因信息
蛋白序列
说明书与实验方案
研究信息

関連項目

附加IRF抗体，包括IRF-1, IRF-2, IRF-3, IRF-4, IRF-5, IRF-6, IRF-7, IRF-2BP1和IRF-2BP

IRF-7抗体（F-1）是一种小鼠单克隆IgG2a kappa轻链抗体，可通过蛋白质印迹（WB）、免疫沉淀（IP）、免疫荧光（IF）和酶联免疫吸附测定（ELISA）等方法检测小鼠、大鼠和人类样本中的IRF-7。抗IRF-7抗体（F-1）有非结合型和多种结合型，包括琼脂糖、辣根过氧化物酶（HRP）、藻红蛋白（PE）、异硫氰酸荧光素（FITC）和多种Alexa Fluor®结合物。干扰素调节因子7（IRF-7）通过调节I型干扰素在免疫反应中发挥关键作用，而I型干扰素对于抗病毒防御至关重要。IRF-7主要位于细胞核中，在病毒感染时激活，对于干扰素-α和干扰素-β基因的转录激活至关重要。这种核定位使IRF-7能够快速响应病毒信号并启动免疫反应，增强宿主抵抗感染的能力。IRF-7与干扰素信号传导通路中的其他蛋白相互作用，包括IRF-3，这进一步强调了IRF-7在调节免疫反应中的重要性。IRF-7形成的蛋白复合物在微调免疫反应中起着关键作用，这使得IRF-7成为免疫学和病毒学研究的重要目标。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

订购信息

产品名称产品编号规格价格数量收藏夹

IRF-7 抗体 (F-1)

sc-74471
200 µg/ml
RMB2377.00

IRF-7 (F-1): m-IgG Fc BP-HRP 套装

sc-525718
200 µg Ab; 10 µg BP
RMB2662.00

IRF-7 (F-1): m-IgG2a BP-HRP 套装

sc-546378
200 µg Ab; 10 µg BP
RMB2662.00

IRF-7 抗体 (F-1) X

sc-74471 X
200 µg/0.1 ml
RMB2377.00

IRF-7 抗体 (F-1) AC

sc-74471 AC
500 µg/ml, 25% agarose
RMB3129.00

IRF-7 抗体 (F-1) HRP

sc-74471 HRP
200 µg/ml
RMB2377.00

IRF-7 抗体 (F-1) FITC

sc-74471 FITC
200 µg/ml
RMB2482.00

IRF-7 抗体 (F-1) PE

sc-74471 PE
200 µg/ml
RMB2580.00

IRF-7 抗体 (F-1) Alexa Fluor® 488

sc-74471 AF488
200 µg/ml
RMB2685.00

IRF-7 抗体 (F-1) Alexa Fluor® 546

sc-74471 AF546
200 µg/ml
RMB2685.00

IRF-7 抗体 (F-1) Alexa Fluor® 594

sc-74471 AF594
200 µg/ml
RMB2685.00

IRF-7 抗体 (F-1) Alexa Fluor® 647

sc-74471 AF647
200 µg/ml
RMB2685.00

IRF-7 抗体 (F-1) Alexa Fluor® 680

sc-74471 AF680
200 µg/ml
RMB2685.00

IRF-7 抗体 (F-1) Alexa Fluor® 790

sc-74471 AF790
200 µg/ml
RMB2685.00

I use this antibody to detect IRF7 in mouse BMDCs. But I do not get bands at the predicted size (>50kDa) but a strong band at ~30kDa and a clear band at ~47kDa (clearly smaller than the 50kDa marker band). Is this IRF7 or something else?

Asked by: Patricia123
Thank you for your question. Please contact our technical service team directly so we can assist you with your experiment.
Answered by: Tech Support Europe
Date published: 2022-07-03

I would like to know if it antibody works very well to detect IRF7 in mouse astrocytes. It is possible to receive a free sample to test it? Thanks a lot,

Asked by: Paola13
Thank you for your question. We have not specifically tested this antibody in astrocytes, however the antibody is confirmed to be reactive with mouse IRF-7. Please contact our Technical Service Department for more information on how to receive a free 10 ug sample of this monoclonal antibody.
Answered by: Technical Support
Date published: 2019-04-24

What is the recommended secondary reagent to use with IRF-7 (F-1): sc-74471 monoclonal antibody for Western blot?

Asked by: cjMara
We recommend using one our exclusive Mouse IgG Binding Proteins as a secondary detection reagent. This primary antibody has a kappa light chain, so for Western blotting we would recommend m-IgGκ BP-HRP: sc-516102. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Answered by: Technical Support 5
Date published: 2017-01-20
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Rated 4 out of 5 by from Good antibodyThis antibody works well in western blot of whole cell extracts prepared from several human cell lines.
Date published: 2021-05-31
Rated 5 out of 5 by from Works well in WB.Works well with fibroblast lysate in 1:200 dilution.
Date published: 2018-05-17
Rated 5 out of 5 by from Good signal on western blotWorks well on western blot. Clear bands between 55kD and 72kD.
Date published: 2017-12-11
Rated 5 out of 5 by from Strong WBGreat product for western blot applications; strong signal
Date published: 2017-02-05
Rated 5 out of 5 by from Good alternative to polyclonalThe F-1 clone is a good alternative to the now discontinued polyclonal, IRF-7 (H-246): sc-9083. It is raised against the same epitope and has been featured in several publications.
Date published: 2016-12-29
Rated 2 out of 5 by from Detecting the IRFDetecting the IRF-7 on hela cells which is infected by virus but the band is not strong.
Date published: 2015-10-29
Rated 4 out of 5 by from Single band at correct mw with little backgroundSingle band at correct mw with little background in Jurkat, PMA-treated Jurkat and Raji whole cell lysates.
Date published: 2013-07-25
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IRF-7 抗体 (F-1) is rated 4.4 out of 5 by 8.
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