Date published: 2025-9-6
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IRF-7 Antibody (G-8) - Western Blotting - Image 389896
IRF-7 抗体 (G-8) HRP: sc-74472 HRP. 对 Jurkat (A), Jurkat + PMA (B), Raji (C), NAMALWA (D) 和 GA-10 (E) 全细胞裂解液中 IRF-7 的表达进行直接 Western 印迹分析.
IRF-7 抗体 (G-8) HRP: sc-74472 HRP. 对 Jurkat (A), Jurkat + PMA (B), Raji (C), NAMALWA (D) 和 GA-10 (E) 全细胞裂解液中 IRF-7 的表达进行直接 Western 印迹分析.

IRF-7 抗体 (G-8): sc-74472

4.7(10)
寫評論提問

说明书
  • IRF-7 抗体 G-8 是小鼠单克隆 IgG2a κ，IRF-7抗体, 在29篇文献中引用，规格为200 µg/ml
  • 免疫human物种的IRF-7的氨基酸1-246
  • IRF-7 抗体 (G-8) 推荐用于WB, IP, IF, IHC(P) 和 ELISA，检测human 来源的 IRF-7
  • 抗IRF-7抗体（G-8）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 可用于凝胶迁移和ChIP的TransCruz试剂(sc-74472 X, 200 µg/0.1 ml)
  • 关于如何获取IRF-7 (G-8): sc-74472的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP 和 m-IgG2a BP-HRP 是 IRF-7 抗体 (G-8) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与IRF-7 抗体 (G-8) 组合成套装提供。 (请参阅下面的订购信息)。

快捷链接

相关产品
描述
基因信息
蛋白序列
说明书与实验方案
研究信息

関連項目

此外 IRF 抗体包括 IRF-1, IRF-2, IRF-3, IRF-4, IRF-5, IRF-6, IRF-7, IRF-2BP1 和 IRF-2BP

IRF-7抗体（G-8）是一种小鼠单克隆IgG2a kappa轻链抗体，可通过蛋白质印迹（WB）、免疫沉淀（IP）、免疫荧光（IF）、石蜡包埋切片免疫组织化学（IHCP）和酶联免疫吸附测定（ELISA）检测人源IRF-7蛋白。IRF-7 (G-8) 抗体有非结合型和多种结合型，包括琼脂糖、辣根过氧化物酶 (HRP)、藻红蛋白 (PE)、异硫氰酸荧光素 (FITC) 和多种 Alexa Fluor® 结合物。IRF-7 通过调节 I 型干扰素的表达在免疫反应中发挥关键作用，而 I 型干扰素对于抗病毒防御至关重要。IRF-7主要位于细胞核中，这种转录因子可激活干扰素-α和干扰素-β基因的表达，以应对病毒感染。核定位能够直接影响细胞转录机制，增强干扰素的产生，从而协调免疫反应。激活后的核转运强调了IRF-7在宿主防御病原体的信号通路中的重要性。抗IRF-7抗体（G-8）使研究人员能够深入了解免疫调节机制和干扰素在抗病毒免疫中的作用。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

IRF-7 抗体 (G-8) 参考文献:

  1. 干扰素调节因子：下一代。  |  Mamane, Y., et al. 1999. Gene. 237: 1-14. PMID: 10524230
  2. IRF-7介导的正反馈增强和多样化IFN诱导。  |  Levy, DE., et al. 2002. J Interferon Cytokine Res. 22: 87-93. PMID: 11846979
  3. 干扰素调节因子 7 调节 Epstein-Barr 病毒潜伏膜蛋白 1 的表达：一个调节回路。  |  Ning, S., et al. 2003. J Virol. 77: 9359-68. PMID: 12915551
  4. 卡波西氏肉瘤相关疱疹病毒编码的vIRF-3可刺激细胞IRF-3和IRF-7的转录活性。  |  Lubyova, B., et al. 2004. J Biol Chem. 279: 7643-54. PMID: 14668346
  5. 在病毒感染的先天反应中，IRF-5和IRF-7的全球和独特靶标。  |  Barnes, BJ., et al. 2004. J Biol Chem. 279: 45194-207. PMID: 15308637
  6. 通过细胞类型特异性蛋白质稳定性调节 IRF7。  |  Prakash, A. and Levy, DE. 2006. Biochem Biophys Res Commun. 342: 50-6. PMID: 16472772
  7. 原代巨噬细胞的转录重编程揭示了 IRF-3 和 IRF-7 不同的凋亡和抗肿瘤功能。  |  Goubau, D., et al. 2009. Eur J Immunol. 39: 527-40. PMID: 19152337
  8. IRF-7的自我抑制机制以及卡波西氏肉瘤相关疱疹病毒蛋白ORF45灭活IRF-7的可能模型。  |  Sathish, N., et al. 2011. J Biol Chem. 286: 746-56. PMID: 20980251
  9. apo IRF-3 和 IRF-7 DNA 结合域的结构：环 L1 对 DNA 结合的影响。  |  De Ioannes, P., et al. 2011. Nucleic Acids Res. 39: 7300-7. PMID: 21596780
  10. CCL7和IRF-7介导鼻病毒1B感染后的标志性炎症和IFN反应。  |  Girkin, J., et al. 2015. J Immunol. 194: 4924-30. PMID: 25847975
  11. Jak-STAT 通路和转录激活对 IFNs 及其他细胞外信号蛋白的反应。  |  Darnell, JE., et al. 1994. Science. 264: 1415-21. PMID: 8197455
  12. IRF-7，一种与 Epstein-Barr 病毒潜伏期相关的新型干扰素调节因子。  |  Zhang, L. and Pagano, JS. 1997. Mol Cell Biol. 17: 5748-57. PMID: 9315633

订购信息

产品名称产品编号规格价格数量收藏夹

IRF-7 抗体 (G-8)

sc-74472
200 µg/ml
RMB2377.00

IRF-7 (G-8): m-IgG Fc BP-HRP 套装

sc-525719
200 µg Ab; 10 µg BP
RMB2662.00

IRF-7 (G-8): m-IgG2a BP-HRP 套装

sc-546379
200 µg Ab; 10 µg BP
RMB2662.00

IRF-7 抗体 (G-8) X

sc-74472 X
200 µg/0.1 ml
RMB2377.00

IRF-7 抗体 (G-8) AC

sc-74472 AC
500 µg/ml, 25% agarose
RMB3129.00

IRF-7 抗体 (G-8) HRP

sc-74472 HRP
200 µg/ml
RMB2377.00

IRF-7 抗体 (G-8) FITC

sc-74472 FITC
200 µg/ml
RMB2482.00

IRF-7 抗体 (G-8) PE

sc-74472 PE
200 µg/ml
RMB2580.00

IRF-7 抗体 (G-8) Alexa Fluor® 488

sc-74472 AF488
200 µg/ml
RMB2685.00

IRF-7 抗体 (G-8) Alexa Fluor® 546

sc-74472 AF546
200 µg/ml
RMB2685.00

IRF-7 抗体 (G-8) Alexa Fluor® 594

sc-74472 AF594
200 µg/ml
RMB2685.00

IRF-7 抗体 (G-8) Alexa Fluor® 647

sc-74472 AF647
200 µg/ml
RMB2685.00

IRF-7 抗体 (G-8) Alexa Fluor® 680

sc-74472 AF680
200 µg/ml
RMB2685.00

IRF-7 抗体 (G-8) Alexa Fluor® 790

sc-74472 AF790
200 µg/ml
RMB2685.00

Hi, Please do you know whether this antibody works fine for the detection of Equine IRF-7 protein using WB? Thank you

Asked by: Fatai
Thank you for your question. We have not tested this antibody, IRF-7 (G-8): sc-74472, in equine samples, and do not know if it will work for that species. The antibody is raised against an antigen corresponding to amino acids 1-246 of IRF-7 of human origin, which shares ~65% sequence identity with a predicted protein sequence for interferon regulatory factor 7-like in Equus caballus (protein accession number: XP_001494207). This is below our typical threshold for recommended cross-reactivity in another species, but it is significant and the antibody may have an epitope that would work for equine. For a limited time, we are offering a free 10 µg sample of this antibody. Please contact our Technical Service Department if you would like to request a free sample to test in your species. Please call us toll-free at (800) 457-3801 option 2, e-mail <[email protected]>, or use the live chat on our website.
Answered by: Technical Support
Date published: 2017-08-21

What is the recommended secondary reagent to be used with this mouse monoclonal primary?

Asked by: jerojero
We recommend using one our exclusive Mouse IgG Binding Proteins as a secondary detection reagent. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Answered by: Technical Support 9
Date published: 2017-01-17
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Rated 5 out of 5 by from Works well in WB.Works well with fibroblast lysate in 1:200 dilution.
Date published: 2018-05-17
Rated 4 out of 5 by from Bands in HEK293 cellsClear bands in HEK293 cell lysates using 20ug of protein (dilution 1:250)
Date published: 2018-02-20
Rated 5 out of 5 by from Good antibodyI used this antibody and nonspecific band were detected! It's a good one.
Date published: 2017-03-16
Rated 5 out of 5 by from Great IRF-7 antibodyIRF-7 (G-8) gave beautiful staining of HeLa cells for Immunofluorescence (IF).
Date published: 2017-01-23
Rated 5 out of 5 by from Great antibodyStrong antibody for both western blot and IF staining
Date published: 2017-01-23
Rated 4 out of 5 by from goodWorked well in WB in human 293T whole cell lysates, Strong band in WB
Date published: 2016-10-28
Rated 5 out of 5 by from Performed well in IF with methanolPerformed well in IF with methanol-fixed HeLa cells showed cytoplasmic and nuclear localization. -SCBT QC
Date published: 2015-05-13
Rated 5 out of 5 by from Worked well with formalin fixedWorked well with formalin fixed, paraffin-embedded human fallopian tube tissue showed nuclear staining of glandular cells at low and high magnification. -SCBT QC
Date published: 2015-01-05
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IRF-7 抗体 (G-8) is rated 4.7 out of 5 by 10.
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