Date published: 2025-9-7
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IP3R-II Antibody (A-5) - Western Blotting - Image 363448
IP3R-II 抗体 (A-5): sc-398434. Hep G2 (A), Jurkat (B) 和 NIH/3T3 (C) 全细胞裂解液及大鼠胸腺组织提取物 (D) 中 IP3R-II 表达的 Western 印迹分析.
IP3R-II 抗体 (A-5): sc-398434. Hep G2 (A), Jurkat (B) 和 NIH/3T3 (C) 全细胞裂解液及大鼠胸腺组织提取物 (D) 中 IP3R-II 表达的 Western 印迹分析.

IP3R-II 抗体 (A-5): sc-398434

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说明书
  • IP3R-II抗体(A-5)是小鼠单克隆IgG2b κ, 在25篇文献中引用，规格为200 µg/ml
  • 特异性抗原位于human物种的IP3R-II的C-terminus的氨基酸2666-2695之间
  • IP3R-II 抗体 (A-5) 推荐用于WB, IP, IF 和 ELISA ，检测 mouse, rat, human 和 来源的IP3R-II; 也和以下物种反应，包括： 和 equine, canine, bovine, porcine and avian
  • 抗IP3R-II抗体（A-5）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取IP3R-II (A-5): sc-398434的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP、 2b BP-HRP">m-IgG2b BP-HRP和m-IgGκ BP-HRP是IP3R-II Antibody (A-5) 适用于 WB 应用。 的首选辅助检测试剂。这些试剂现与IP3R-II Antibody (A-5) 打包提供（请参阅下面的订购信息）。

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描述
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说明书与实验方案
研究信息

関連項目

额外IP3R抗体，包括IP3R-I, IP3R-II和IP3R-III

IP3R-II抗体（A-5）是一种IgG2b κ小鼠单克隆IP3R-II抗体（也被称为IP3R-II抗体或ITPR2抗体），能够通过蛋白质印迹（WB）、免疫沉淀（IP）、免疫荧光（IF）和酶联免疫吸附测定（ELISA）检测来自小鼠、大鼠和人类的IP3R-II。IP3R-II抗体（A-5）既有非偶联形式的抗IP3R-II抗体，也有多种偶联形式的抗IP3R-II抗体，包括琼脂糖、辣根过氧化物酶（HRP）、藻红蛋白（PE）、异硫氰酸荧光素（FITC）和多种Alexa Fluor®偶联物。肌醇1,4,5-三磷酸（IP3）作为第二信使，对激素、生长因子和神经递质等无数细胞外刺激产生反应。受体酪氨酸激酶通过激活磷脂酶（如磷脂酶C，PLC）等间接增加细胞内IP3水平，将磷脂酰肌醇-4,5-二磷酸转化为IP3和二酰甘油（DAG）。肌醇1,4,5-三磷酸受体（IP3R）在各种细胞类型中作为肌醇三磷酸（IP3）门控的钙释放通道起作用。已描述了三种IP3受体亚型，分别命名为IP3R-I、IP3R-II和IP3R-III。IP3R-I是在神经元组织和中枢神经系统中表达的主要IP3R亚型，但在肝脏中也有高水平表达。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

IP3R-II 抗体 (A-5) 参考文献:

  1. 在局灶性缺血性中风后，星形胶质细胞中IP₃R2介导的Ca²⁺信号传导途径的破坏可减轻神经元死亡和脑损伤，同时减少行为缺陷。  |  Li, H., et al. 2015. Cell Calcium. 58: 565-76. PMID: 26433454
  2. DPP4的非酶功能通过IGF-2R/PKA/SP1/ERp29/IPCR2途径介导的Treg功能和M1小胶质细胞极化损伤促进糖尿病相关的认知功能障碍。  |  Hui, Y., et al. 2023. Metabolism. 138: 155340. PMID: 36302455
  3. IP3R2介导的Ca2+释放通过激活NLRP3/Caspase-1/GSDMD途径促进LPS诱导的心肌细胞热解。  |  Wu, QR., et al. 2024. Cell Death Discov. 10: 91. PMID: 38378646
  4. IP3R2通过线粒体-ER接触调节缺氧光感受器损伤中的Ca2+转移。  |  Xu, L., et al. 2024. Exp Eye Res. 245: 109965. PMID: 38851477
  5. 短暂局灶性脑缺血后大鼠脑内 IP3 受体和 IP3-3-kinase mRNA 表达的原位杂交。  |  Zhang, SX., et al. 1995. Brain Res Mol Brain Res. 32: 252-60. PMID: 7500836
  6. 果蝇中 1,4,5-三磷酸肌醇受体的表达表明 IP3 信号在肌肉发育和成体化学感觉功能中发挥作用。  |  Raghu, P. and Hasan, G. 1995. Dev Biol. 171: 564-77. PMID: 7556937
  7. WB 大鼠肝上皮细胞中 I 型和 III 型三磷酸肌醇受体的异构体。  |  Joseph, SK., et al. 1995. J Biol Chem. 270: 23310-6. PMID: 7559486
  8. 在胰岛, 肾脏, 胃肠道和其他组织中表达的第三种三磷酸肌醇受体亚型 IP3R-3 的序列和功能特征。  |  Blondel, O., et al. 1993. J Biol Chem. 268: 11356-63. PMID: 8388391
  9. 与 1,4,5-三磷酸肌醇受体-FKBP12 复合物缔合作用的钙调素调节 Ca2+ 通量。  |  Cameron, AM., et al. 1995. Cell. 83: 463-72. PMID: 8521476
  10. 缺乏 1 型肌醇-1,4,5-三磷酸受体的小鼠共济失调和癫痫发作。  |  Matsumoto, M., et al. 1996. Nature. 379: 168-71. PMID: 8538767
  11. 通过酪氨酸磷酸化调节 1,4,5-三磷酸肌醇受体。  |  Jayaraman, T., et al. 1996. Science. 272: 1492-4. PMID: 8633244
  12. 淋巴细胞凋亡：3 型 1,4,5-三磷酸肌醇受体增加的调解作用。  |  Khan, AA., et al. 1996. Science. 273: 503-7. PMID: 8662540

订购信息

产品名称产品编号规格价格数量收藏夹

IP3R-II 抗体 (A-5)

sc-398434
200 µg/ml
RMB2377.00

IP3R-II (A-5): m-IgG Fc BP-HRP 套装

sc-530795
200 µg Ab; 10 µg BP
RMB2662.00

IP3R-II (A-5): m-IgGκ BP-HRP 套装

sc-524338
200 µg Ab, 40 µg BP
RMB2662.00

IP3R-II (A-5): m-IgG2b BP-HRP 套装

sc-550227
200 µg Ab; 10 µg BP
RMB2662.00

IP3R-II 抗体 (A-5) AC

sc-398434 AC
500 µg/ml, 25% agarose
RMB3129.00

IP3R-II 抗体 (A-5) HRP

sc-398434 HRP
200 µg/ml
RMB2377.00

IP3R-II 抗体 (A-5) FITC

sc-398434 FITC
200 µg/ml
RMB2482.00

IP3R-II 抗体 (A-5) PE

sc-398434 PE
200 µg/ml
RMB2580.00

IP3R-II 抗体 (A-5) Alexa Fluor® 488

sc-398434 AF488
200 µg/ml
RMB2685.00

IP3R-II 抗体 (A-5) Alexa Fluor® 546

sc-398434 AF546
200 µg/ml
RMB2685.00

IP3R-II 抗体 (A-5) Alexa Fluor® 594

sc-398434 AF594
200 µg/ml
RMB2685.00

IP3R-II 抗体 (A-5) Alexa Fluor® 647

sc-398434 AF647
200 µg/ml
RMB2685.00

IP3R-II 抗体 (A-5) Alexa Fluor® 680

sc-398434 AF680
200 µg/ml
RMB2685.00

IP3R-II 抗体 (A-5) Alexa Fluor® 790

sc-398434 AF790
200 µg/ml
RMB2685.00

IP3R-II (A-5) 中和勝肽

sc-398434 P
100 µg/0.5 ml
RMB511.00

Have any researchers used this antibody to perform WB on rat heart tissue?

Asked by: 慈悲客栈
Thank you for your question. sc-398434 is recommended for detection of rat samples by WB. Please refer to its citations by visiting the link: https://www.scbt.com/zh/p/ip3r-ii-antibody-a-5#citations.
Answered by: Technical Service SH
Date published: 2025-08-19

这是鼠源的嘛

Asked by: zzzz
感谢您的提问。IP3R-II 抗体 (A-5): sc-398434是小鼠来源。 Thank you for your question. The origin of IP3R-II antibody (A-5): sc-398434 is mouse.
Answered by: Technical Service SH
Date published: 2025-08-07

I need to use an antibody to detect the protein in samples of avian origin. Do you know if this mouse monoclonal antibody will work?

Asked by: jerojero
Please contact our Technical Service Department and we would be happy to perform sequence analysis and review our data to answer any questions you may have about additional species reactivity.
Answered by: Technical Support 14
Date published: 2017-02-04
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Rated 5 out of 5 by from IP3R-II antibodyThis mouse antibody was very good and gave me a good experience. I will consider this company's product next time.
Date published: 2023-12-21
Rated 4 out of 5 by from Worked well!Worked quite well in WT HEK293T cells, which are known to express IP3R2. Used at 1:1000 dilution in 3% BSA in TBS-T (0.1%) for 24hrs at 4C. Loaded 40,80,120ug whole cell lysate per well (extracted with 1% Triton lysis buffer)
Date published: 2021-08-25
Rated 5 out of 5 by from Very nice Ab!1h blocking in mPBST, IP3RII ab 1:1000 ON at 4°C, secondary ab 1h at RT; nice signal detected at the expected size, no unspecific bands in this region
Date published: 2017-11-27
Rated 5 out of 5 by from Results show is very good1:200 dilution at WB, the background is always clean and there is a strong and clear band.
Date published: 2017-10-27
Rated 5 out of 5 by from good antibodyI got this ab a month ago...it worked well in my cell system (kidney cells)
Date published: 2017-09-16
Rated 4 out of 5 by from hard to get high molecular protein bandsWith sc-398434 mouse monoclonal antibody we've got good bands
Date published: 2017-03-16
Rated 5 out of 5 by from specific resultsIP3R-II gives clean and specific results in WB when used detect mouse tissues.
Date published: 2017-03-11
Rated 5 out of 5 by from I am satisfied with the result.I used this antibody in human lung tissue to do Western blot dilution 1:1000, the effect is very good. I am satisfied with the result.
Date published: 2017-02-09
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IP3R-II 抗体 (A-5) is rated 4.8 out of 5 by 11.
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