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Integrin β1/ITGB1 Antibody (JB1B) - Western Blotting - Image 378220
Integrin β1抗体 (JB1B): sc-59829. 对 HeLa (A), U-87 MG (B), A549 (C) 和 A-375 (D) 全细胞裂解液中 Integrin β1 的表达进行 Western 印迹分析. 所用检测试剂: m-IgGκ BP-HRP: sc-516102.
Integrin β1抗体 (JB1B): sc-59829. 对 HeLa (A), U-87 MG (B), A549 (C) 和 A-375 (D) 全细胞裂解液中 Integrin β1 的表达进行 Western 印迹分析. 所用检测试剂: m-IgGκ BP-HRP: sc-516102.

Integrin β1/ITGB1 抗体 (JB1B): sc-59829

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寫評論提問

说明书
  • Integrin β1/ITGB1抗体(JB1B)是小鼠单克隆IgG2a κ, 在20篇文献中引用，规格为200 µg/ml
  • 免疫human物种的Integrin β1
  • Integrin β1/ITGB1 抗体 (JB1B) 推荐用于WB, IP, IF 和 FCM，检测human 来源的 Integrin β1
  • 抗Integrin β1/ITGB1抗体（JB1B）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取Integrin β1/ITGB1 (JB1B): sc-59829的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP、 2a BP-HRP">m-IgG2a BP-HRP和m-IgGκ BP-HRP是Integrin β1/ITGB1 Antibody (JB1B) 适用于 WB 应用。 的首选辅助检测试剂。这些试剂现与Integrin β1/ITGB1 Antibody (JB1B) 打包提供（请参阅下面的订购信息）。

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描述
基因信息
说明书与实验方案
研究信息

関連項目

附加Integrin抗体，包括Integrin α 1, Integrin α 11, Integrin α 2, Integrin α 3, Integrin α 4, Integrin α 5, Integrin α 6, Integrin α 7, Integrin α 8, Integrin α 9, Integrin α E, Integrin α IIb, Integrin α IIIa, Integrin α IIIb, Integrin α L和Integrin α M

整合素β1抗体（JB1B）是一种小鼠单克隆IgG2a kappa轻链抗体，可通过蛋白质印迹（WB）、免疫沉淀（IP）、免疫荧光（IF）和流式细胞术（FCM）检测人类整合素β1。Integrin β1 (JB1B) 抗体有非结合型和多种结合型，包括琼脂糖、辣根过氧化物酶 (HRP)、藻红蛋白 (PE)、异硫氰酸荧光素 (FITC) 和多种 Alexa Fluor® 结合物。Integrin β1 在细胞粘附和信号传导中起着至关重要的作用，是细胞外基质相互作用的关键组成部分。整合素β1主要位于细胞表面，与各种α亚基形成异二聚体，从而与纤维蛋白原、胶原和纤维蛋白结合。这种结合对于细胞迁移、增殖和存活等过程至关重要，因为结合有助于细胞外环境与细胞内信号通路之间的沟通。整合素β1能够聚集并激活信号级联，这对于维持组织完整性和应对机械应力至关重要，因此整合素β1在伤口愈合和免疫反应中发挥着重要作用。抗整合素β1抗体（JB1B）使研究人员能够深入了解整合素在细胞行为中的动态作用及其在包括癌症和纤维化在内的各种疾病中的影响。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

订购信息

产品名称产品编号规格价格数量收藏夹

Integrin β1/ITGB1 抗体 (JB1B)

sc-59829
200 µg/ml
RMB2377.00

Integrin β1/ITGB1 (JB1B): m-IgG Fc BP-HRP 套装

sc-528639
200 µg Ab; 10 µg BP
RMB2662.00

Integrin β1/ITGB1 (JB1B): m-IgGκ BP-HRP 套装

sc-521071
200 µg Ab, 40 µg BP
RMB2662.00

Integrin β1/ITGB1 (JB1B): m-IgG2a BP-HRP 套装

sc-547132
200 µg Ab; 10 µg BP
RMB2662.00

Integrin β1/ITGB1 抗体 (JB1B) AC

sc-59829 AC
500 µg/ml, 25% agarose
RMB3129.00

Integrin β1/ITGB1 抗体 (JB1B) HRP

sc-59829 HRP
200 µg/ml
RMB2377.00

Integrin β1/ITGB1 抗体 (JB1B) FITC

sc-59829 FITC
200 µg/ml
RMB2482.00

Integrin β1/ITGB1 抗体 (JB1B) PE

sc-59829 PE
200 µg/ml
RMB2580.00

Integrin β1/ITGB1 抗体 (JB1B) Alexa Fluor® 488

sc-59829 AF488
200 µg/ml
RMB2685.00

Integrin β1/ITGB1 抗体 (JB1B) Alexa Fluor® 546

sc-59829 AF546
200 µg/ml
RMB2685.00

Integrin β1/ITGB1 抗体 (JB1B) Alexa Fluor® 594

sc-59829 AF594
200 µg/ml
RMB2685.00

Integrin β1/ITGB1 抗体 (JB1B) Alexa Fluor® 647

sc-59829 AF647
200 µg/ml
RMB2685.00

Integrin β1/ITGB1 抗体 (JB1B) Alexa Fluor® 680

sc-59829 AF680
200 µg/ml
RMB2685.00

Integrin β1/ITGB1 抗体 (JB1B) Alexa Fluor® 790

sc-59829 AF790
200 µg/ml
RMB2685.00

Can Integrin β1 (JB1B): sc-59829 monoclonal antibody be used in IF or IHC with mouse tissue or cells? Will there be non-specific staining?

Asked by: Randy McDonald
Thank you for your inquiry. The use of mouse monoclonal antibodies with mouse samples is very common and typically poses no problem. To eliminate any potential cross-reactivity of an anti-mouse secondary detection reagent, Integrin β1 (JB1B): sc-59829 is available in a variety of direct conjugations, such as HRP, PE, FITC and AlexaFluors.
Answered by: Technical Support
Date published: 2017-02-24
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Rated 3 out of 5 by from IHC Assay Review for Integrin B1 mABApplication: Immunohistochemistry. Protocol: Standard protocol for saliva smear staining. Dilutions tested: 1:10, 1:30, 1:90, 1:270. Results: Weak but specific epithelial and lymphocyte stain at 1:10. Residual low stain at 1:30.
Date published: 2017-08-31
Rated 5 out of 5 by from Very good antibodyWestern blot 1/500, very smooth experiment, very clean.
Date published: 2017-08-16
Rated 5 out of 5 by from Good effectI have used this antibody for western blot and seen good bands, the result is very good.
Date published: 2017-08-15
Rated 5 out of 5 by from good result.the Integrin beta 1 mouse clonal antibody. Works good in Western for human samples detection 1:600.
Date published: 2017-02-25
Rated 5 out of 5 by from Great stainingExcellent results in IF and also in Flow Cytometry!
Date published: 2017-01-17
Rated 5 out of 5 by from very goodsc-59829 at 1/500 staining Integrin beta 1 antibody in human transitional cell carcinoma of bladder by immunohistochemistry,this antibody staining is very good.
Date published: 2017-01-07
Rated 4 out of 5 by from Potent and very specific antibodyThis monoclonal antibody was independently tested in-house by Kinexus Bioinformatics Corporation and validated for use for Western blotting. There was medium detection of the ~138 kDa target protein in a HeLa/Jurkat cell lysate mix, but not detected in mouse tissues (brain/testes/thymus/muscle mix). No major cross-reactivities were observed at 2 µg/ml antibody concentration with off-target proteins in the mouse tissues tested or in the HeLa/Jurkat cell lysate mix.
Date published: 2016-11-10
Rated 5 out of 5 by from Publication with HT1080 cells in WBPublication with HT1080 cells in WB -SCBT Publication Review
Date published: 2015-04-08
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Integrin β1/ITGB1 抗体 (JB1B) is rated 4.8 out of 5 by 13.
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