Date published: 2025-9-6
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Integrin αV/β3/CD51/CD61 Antibody (23C6) - Flow Cytometry - Image 6076
Integrin αV/β3 (23C6) PE: sc-7312 PE. 人体外周血白细胞的 FCM 分析. 黑线直方图代表同型对照, 正常小鼠 IgG1: sc-2866.
Integrin αV/β3/CD51/CD61 Antibody (23C6) - Flow Cytometry - Image 6076
Integrin αV/β3 (23C6) PE: sc-7312 PE. 人体外周血白细胞的 FCM 分析. 黑线直方图代表同型对照, 正常小鼠 IgG1: sc-2866.
Integrin αV/β3/CD51/CD61 Antibody (23C6) - Immunohistochemistry - Image 156486
整合素 aV/b3 (23C6): sc-7312. 福尔马林固定, 石蜡包埋的人膀胱组织的免疫过氧化物酶染色显示尿道细胞膜染色.
Integrin αV/β3 (23C6) PE: sc-7312 PE. 人体外周血白细胞的 FCM 分析. 黑线直方图代表同型对照, 正常小鼠 IgG1: sc-2866.

Integrin αV/β3/CD51/CD61 抗体 (23C6): sc-7312

4.7(6)
寫評論提問

说明书
  • Integrin αV/β3/CD51/CD61抗体(23C6)是小鼠单克隆IgG1 κ, 在112篇文献中引用，规格为200 µg/ml
  • 针对来自human来源的破骨细胞瘤的破骨细胞
  • 抗-Integrin αV/β3/CD51/CD61 抗体 (23C6) 推荐用于 IP, IF, IHC(P) 和 FCM，检测mouse, rat, human, African green monkey, Cynomolgus (Macaca fascicularis), chicken, bovine, rabbit 和porcine 来源的 Integrin αV/β3
  • Integrin αV/β3/CD51/CD61 抗体 (23C6) 可偶联 藻红蛋白 或者 FITC 用于 IF, IHC(P) 和 FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 还可偶联Alexa Fluor® 405 用于IF, IHC(P) 和FCM
  • 关于如何获取Integrin αV/β3/CD51/CD61 (23C6): sc-7312的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • 1 BP-HRP">m-IgG1 BP-HRP是Integrin αV/β3/CD51/CD61 Antibody (23C6) 适用于 IHC(P) 应用。 的首选辅助检测试剂。该试剂现与Integrin αV/β3/CD51/CD61 Antibody (23C6) 打包提供（请参阅下面的订购信息）。

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描述
基因信息
说明书与实验方案
研究信息

関連項目

额外Integrin抗体，包括Integrin α 1, Integrin α 11, Integrin α 2, Integrin α 3, Integrin α 4, Integrin α 5, Integrin α 6, Integrin α 7, Integrin α 8, Integrin α 9, Integrin α E, Integrin α IIb, Integrin α IIIa, Integrin α IIIb, Integrin α L和Integrin α M

Integrin αV/β3 抗体（23C6）是一种小鼠单克隆 IgG1 kappa 轻链抗体，可通过免疫沉淀（IP）、免疫荧光（IF）、石蜡包埋切片免疫组织化学（IHCP）和流式细胞术检测小鼠、大鼠、人、非洲绿猴、食蟹猴 olgus（Macaca fascicularis）、鸡、牛、兔和猪，通过免疫沉淀（IP）、免疫荧光（IF）、石蜡包埋切片免疫组织化学（IHCP）和流式细胞术（FCM）检测。抗整合素αV/β3抗体（23C6）有结合和非结合两种形式，可满足各种实验需求。包括αV/β3在内的整合素对于介导细胞粘附至关重要，在细胞信号传导通路中发挥着重要作用。整合素αV/β3的结构是一个由非共价结合的α和β亚基组成的异源二聚体，能够与纤维粘连蛋白和玻璃粘连蛋白等细胞外基质中的多种配体以及纤维蛋白原等可溶性配体结合。这种结构多样性使得整合素能够参与细胞的迁移、增殖和存活，因此这些蛋白质对于组织发育和修复至关重要。整合素αV/β3与其他蛋白质（如细胞内粘附分子（ICAM））的相互作用凸显了其在细胞间相互作用和免疫反应中的重要性。在研究中使用整合素αV/β3 (23C6)抗体，可以深入了解细胞粘附和信号传导机制，这对于理解各种生理和病理过程至关重要。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

Integrin αV/β3/CD51/CD61 抗体 (23C6) 参考文献:

  1. 整合素：细胞粘附中的多功能性, 调节和信号传递。  |  Hynes, RO. 1992. Cell. 69: 11-25. PMID: 1555235
  2. 基于 RGD 的 PET 示踪剂用于受体整合素 αv β3 表达的成像。  |  Cai, H. and Conti, PS. 2013. J Labelled Comp Radiopharm. 56: 264-79. PMID: 24285371
  3. 癌相关成纤维细胞（CAF）衍生的IL32通过整合素β3-p38 MAPK信号传导促进乳腺癌细胞的侵袭和转移。  |  Wen, S., et al. 2019. Cancer Lett. 442: 320-332. PMID: 30391782
  4. 白细胞介素-8通过PI3K/Akt途径促进肝细胞癌整合素β3上调和细胞侵袭  |  Sun, F., et al. 2019. J Exp Clin Cancer Res. 38: 449. PMID: 31684995
  5. 整合素β3通过调节PTEN/Akt/mTOR和ERK1/2途径促进心肌细胞增殖并减轻缺氧诱导的细胞凋亡  |  Wei, L., et al. 2020. Int J Biol Sci. 16: 644-654. PMID: 32025212
  6. 整合素β3-PKM2通路介导的有氧糖酵解导致机械通气引起的肺纤维化。  |  Mei, S., et al. 2022. Theranostics. 12: 6057-6068. PMID: 36168620
  7. 阻断髌下脂肪垫中的骨桥蛋白-整合素 β3 信号传导可减轻小鼠骨关节炎。  |  Dai, B., et al. 2023. Adv Sci (Weinh). 10: e2300897. PMID: 37218542
  8. 血小板衍生的微粒可转移整合素β3，从而促进肿瘤浸润性T细胞的抗肿瘤作用。  |  Zhou, M., et al. 2024. Oncoimmunology. 13: 2304963. PMID: 38235317
  9. 整合素和信号转导途径：走过的路。  |  Clark, EA. and Brugge, JS. 1995. Science. 268: 233-9. PMID: 7716514
  10. 受体占据和聚集在整合素跨膜功能中的协同作用  |  Miyamoto, S., et al. 1995. Science. 267: 883-5. PMID: 7846531
  11. 上皮整合素  |  Sheppard, D. 1996. Bioessays. 18: 655-60. PMID: 8760339
  12. 可溶性因子与整合素介导的细胞锚定在控制细胞生长和分化中的合作。  |  Juliano, R. 1996. Bioessays. 18: 911-7. PMID: 8939069

订购信息

产品名称产品编号规格价格数量收藏夹

Integrin αV/β3/CD51/CD61 抗体 (23C6)

sc-7312
200 µg/ml
RMB2377.00

Integrin αV/β3/CD51/CD61 (23C6): m-IgG1 BP-HRP 套装

sc-531627
200 µg Ab; 20 µg BP
RMB2662.00

Integrin αV/β3/CD51/CD61 抗体 (23C6) FITC

sc-7312 FITC
200 µg/ml
RMB2482.00

Integrin αV/β3/CD51/CD61 抗体 (23C6) PE

sc-7312 PE
200 µg/ml
RMB2580.00

Integrin αV/β3/CD51/CD61 抗体 (23C6) Alexa Fluor® 488

sc-7312 AF488
200 µg/ml
RMB2685.00

Integrin αV/β3/CD51/CD61 抗体 (23C6) Alexa Fluor® 546

sc-7312 AF546
200 µg/ml
RMB2685.00

Integrin αV/β3/CD51/CD61 抗体 (23C6) Alexa Fluor® 594

sc-7312 AF594
200 µg/ml
RMB2685.00

Integrin αV/β3/CD51/CD61 抗体 (23C6) Alexa Fluor® 647

sc-7312 AF647
200 µg/ml
RMB2685.00

Integrin αV/β3/CD51/CD61 抗体 (23C6) Alexa Fluor® 680

sc-7312 AF680
200 µg/ml
RMB2685.00

Integrin αV/β3/CD51/CD61 抗体 (23C6) Alexa Fluor® 790

sc-7312 AF790
200 µg/ml
RMB2685.00

Integrin αV/β3/CD51/CD61 抗体 (23C6) Alexa Fluor® 405

sc-7312 AF405
200 µg/ml
RMB2685.00

I have the integrin alpha V and Beta 3 antibody sc7312 cat no. Can I use it for western blot ? I have attempted 2 times but I'm not getting the desired band.

Asked by: Enquiry for sc7312
Thank you for contacting Santa Cruz Biotechnology. To better assist you please contact technical service. Please call 800-457-3801 or email [email protected].
Answered by: BlakeJ
Date published: 2025-02-06

Mice do not make antibodies to mouse extracellular proteins in the great majority of cases; they are tolerized to them. The antibody used here recognizes mouse is true? Did you verified it?

Asked by: Lianjiu su
Thank you for your question. Yes, we have verified the antibody for mouse species. The antibody is recommended for mouse, rat, human, African green monkey, Cynomolgus (Macaca fascicularis), chicken, bovine, rabbit and porcine.
Answered by: BlakeJ
Date published: 2024-10-24

可以做WB实验吗？

Asked by: 小吴220
感谢您的提问。抗-Integrin αV/β3/CD51/CD61 抗体 (23C6) 推荐用于 IP, IF, IHC(P) 和 FCM。不推荐用于WB实验。谢谢。 Thank you for your question.Anti-Integrin αV/β3/CD51/CD61 Antibody (23C6) is recommended for detection of Integrin αV/β3 by IP, IF, IHC(P) and FCM.Not recommended for WB experiments. Thank you.
Answered by: Jiajun Z
Date published: 2023-08-07

I've been using this antibody for immunohistochemistry on paraffin fixed and embedded samples. We tried different protocols but it doesn't have work, we don't have results. We used HiER recovery (4 seconds at 110°C and 10 seconds at 90°C) Continue part II

Asked by: Jav2022
Thank you for your question. Please contact technical support at [email protected] or call (800)-457-3801.
Answered by: BlakeJ
Date published: 2022-12-15

Part II. Primary antibody incubation of 1:750 and 1:250 overnight at 4°C. And 1:50 during 30 minutes at room temperature. But we don't have any results at any tissue that we tried (placenta, miometrium, endometrium, osteosarcoma and urotelial carcinoma)

Asked by: Jav2022
Thank you for your question. Please contact technical support at [email protected] or call (800)-457-3801.
Answered by: BlakeJ
Date published: 2022-12-15

What are the physical dimensions of the antibody? (ie in nanometers?)

Asked by: mel1100
Thank you for your question. It would be helpful if you could call 800-457-3801, allowing for a more interactive discussion of this and other related questions.
Answered by: Technical Service
Date published: 2022-07-05

这个抗体用于IP时稀释比例是多少呢？

Asked by: xin123123
Thanks for your questions.To 1 ml of the cell lysate, or approximately 100–1000 µg of total cellular protein, add 10 µg of primary antibody agarose conjugate.Alternatively, if primary antibody agarose conjugate is not available, incubate 1 ml cell lysate with 1–10 µl (i.e., 0.2–2 µg) primary antibody (optimal antibody concentration should be determined by titration). Here is the link to protocol for IP for your reference:https://www.scbt.com/zh/resources/protocols/immunoprecipitation-western-blots. 感谢您的提问。100-1000微克的总蛋白，建议10ug的琼脂糖偶联抗体。若使用未偶联琼脂糖的抗体建议0.2-2ug抗体，最佳抗体浓度需要实验滴定。我们公司推荐的IP实验流程见 链接：https://www.scbt.com/zh/resources/protocols/immunoprecipitation-western-blots.
Answered by: Technical Support 9
Date published: 2021-09-07

这个型号的抗体可以用于western blot实验吗

Asked by: dj123
感谢您的提问。Integrin αV/β3/CD51/CD61 抗体 (23C6): sc-7312 不推荐用于western blot 实验。 Thank you for your question. Integrin αV/β3/CD51/CD61 antibody (23C6): sc-7312 is not recommended for western blot.
Answered by: Technical Support 12
Date published: 2021-09-06
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Rated 5 out of 5 by from funziona beneCondizioni di utilizzo: blocking 5% milk 1h Ab I: 1:1000 tTBS ON Ab:II 1:3000 mouse 3%milk è visibile una netta banda tra 100-140 KDa osservata mediante westernblot
Date published: 2022-02-10
Rated 4 out of 5 by from Worked in IFShows nice and specific, but somewhat weak signal in immunofluorescence assay
Date published: 2019-05-14
Rated 4 out of 5 by from Anticorps validéTest de cet anticorps sur lignée humaine (kératinocyte et myoblaste) en cytomètrie en flux -> validé
Date published: 2018-03-21
Rated 5 out of 5 by from great signalGreat signal obtained with alpha V/ beta 3 Antibody (23C6) by FCM and IHC (P)
Date published: 2017-01-18
Rated 5 out of 5 by from Strong ihcNice ihc and flow results;need to see western blot data
Date published: 2017-01-02
Rated 5 out of 5 by from Mouse ES cell lines (E14Tg2a and B6GMouse ES cell lines (E14Tg2a and B6G-2) were investigated with this antibody in flow cytometry. Publishable results produced. No significant cross-reactivity with other proteins was observed -SCBT Publication Review
Date published: 2013-06-03
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Integrin αV/β3/CD51/CD61 抗体 (23C6) is rated 4.7 out of 5 by 6.
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