Date published: 2025-9-8
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Insulin Antibody (2D11-H5) - Immunohistochemistry - Image 347931
胰岛素抗体 (2D11-H5): sc-8033. 福尔马林固定, 石蜡包埋的人胰腺组织的免疫过氧化物酶染色显示朗格汉斯小体的胞浆染色.
胰岛素抗体 (2D11-H5): sc-8033. 福尔马林固定, 石蜡包埋的人胰腺组织的免疫过氧化物酶染色显示朗格汉斯小体的胞浆染色.

Insulin 抗体 (2D11-H5): sc-8033

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说明书
  • Insulin 抗体 2D11-H5 是小鼠单克隆 IgG1 κ，Insulin抗体, 在78篇文献中引用，规格为200 µg/ml
  • 免疫1-84氨基酸，对应于porcine来源Insulin全长
  • Insulin 抗体 (2D11-H5) 推荐用于 IP, IF, IHC(P) 和 ELISA，检测mouse, rat 和human 来源的 Insulin; 也和以下物种反应，包括： 和 bovine and porcine
  • 抗Insulin抗体（2D11-H5）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取Insulin (2D11-H5): sc-8033的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP和 1 BP-HRP">m-IgG1 BP-HRP是Insulin Antibody (2D11-H5) for IHC(P) applications. 的首选辅助检测试剂，这些试剂现与Insulin Antibody (2D11-H5) 打包提供（请参阅下面的订购信息）。

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描述
基因信息
说明书与实验方案
研究信息

関連項目

附加Insulin抗体，包括Insulin B

胰岛素抗体（2D11-H5）是一种小鼠单克隆IgG1 kappa轻链抗体，用于检测多种物种的胰岛素，包括小鼠、大鼠、人、牛和猪，可通过IP、IF、IHCP和ELISA应用。胰岛素小鼠单克隆抗体（2D11-H5）靶向全长猪胰岛素1-84个氨基酸组成的表位，确保牢固可靠的结合。胰岛素是由胰腺合成的一种关键肽类激素，通过促进细胞对葡萄糖的吸收和糖原合成，从而降低血糖浓度，在调节血糖水平方面发挥着至关重要的作用。胰岛素最初以前胰岛素的形式产生，随后加工成胰岛素原，最后形成成熟的胰岛素，由51个氨基酸组成，分为A链和B链，由两个二硫键连接。除了葡萄糖代谢，胰岛素还能提高细胞对单糖、氨基酸和脂肪酸的渗透性，因此对于整体细胞功能和能量平衡至关重要。胰岛素的分泌受到严格调控，在没有外部刺激的情况下，其基础水平得以维持，而当血糖水平升高时，其分泌量也会增加。胰岛素通过与靶细胞膜上的特定受体结合，启动一系列细胞内事件，从而促进代谢活动。胰岛素分泌或信号传导失调可导致高胰岛素血症和2型糖尿病等病症，这凸显了胰岛素在研究和临床中的重要性。胰岛素小鼠单克隆抗体（2D11-H5）适用于研究胰岛素在代谢、作用机制和失调病理学中的多方面作用的研究人员。胰岛素单克隆抗体（2D11-H5）有非结合型和多种结合型，包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®结合物。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

Insulin 抗体 (2D11-H5) 参考文献:

  1. 甜菊糖甙不会像磺酰脲类药物格列本脲那样导致基础胰岛素分泌增加或β细胞脱敏：体外研究。  |  Chen, J., et al. 2006. Life Sci. 78: 1748-53. PMID: 16260001
  2. 动态力量训练可提高肥胖者皮下脂肪组织的胰岛素敏感性以及肾上腺素能α 2A 和β通路之间的功能平衡。  |  Polak, J., et al. 2005. Diabetologia. 48: 2631-40. PMID: 16273345
  3. 一种适用于通过 RIA 法测定人胰岛素 C 肽的单克隆抗体。  |  Hilgert, I., et al. 1991. Hybridoma. 10: 379-86. PMID: 1916848
  4. 胰岛素受体和 IGF-1 受体胞质结构域的信号潜力差异。  |  Lammers, R., et al. 1989. EMBO J. 8: 1369-75. PMID: 2548842
  5. 胰岛素作用的分子机制。  |  Kahn, CR. 1985. Annu Rev Med. 36: 429-51. PMID: 2986528
  6. 超活性单体 des-[Phe(B25)] 人胰岛素突变体的溶液结构：阐明 des-[Phe(B25)] 胰岛素单体化和原生胰岛素二聚化的结构基础。  |  Jørgensen, AM., et al. 1996. J Mol Biol. 257: 684-99. PMID: 8648633
  7. 朗格汉斯胰岛细胞膜钙振荡与胰岛素释放  |  Soria, B. and Martin, F. 1998. Diabetes Metab. 24: 37-40. PMID: 9534007
  8. 原胰岛素：最新观察和争议。  |  Mackin, RB. 1998. Cell Mol Life Sci. 54: 696-702. PMID: 9711235
  9. β细胞葡萄糖敏感性受损和全身胰岛素敏感性受损是非糖尿病患者高血糖的预测因素。  |  Walker, M., et al. 2005. Diabetologia. 48: 2470-6. PMID: 16261308

订购信息

产品名称产品编号规格价格数量收藏夹

Insulin 抗体 (2D11-H5)

sc-8033
200 µg/ml
RMB2377.00

Insulin (2D11-H5): m-IgG Fc BP-HRP 套装

sc-526511
200 µg Ab; 10 µg BP
RMB2662.00

Insulin (2D11-H5): m-IgG1 BP-HRP 套装

sc-531884
200 µg Ab; 20 µg BP
RMB2662.00

Insulin 抗体 (2D11-H5) AC

sc-8033 AC
500 µg/ml, 25% agarose
RMB3129.00

Insulin 抗体 (2D11-H5) HRP

sc-8033 HRP
200 µg/ml
RMB2377.00

Insulin 抗体 (2D11-H5) FITC

sc-8033 FITC
200 µg/ml
RMB2482.00

Insulin 抗体 (2D11-H5) PE

sc-8033 PE
200 µg/ml
RMB2580.00

Insulin 抗体 (2D11-H5) Alexa Fluor® 488

sc-8033 AF488
200 µg/ml
RMB2685.00

Insulin 抗体 (2D11-H5) Alexa Fluor® 546

sc-8033 AF546
200 µg/ml
RMB2685.00

Insulin 抗体 (2D11-H5) Alexa Fluor® 594

sc-8033 AF594
200 µg/ml
RMB2685.00

Insulin 抗体 (2D11-H5) Alexa Fluor® 647

sc-8033 AF647
200 µg/ml
RMB2685.00

Insulin 抗体 (2D11-H5) Alexa Fluor® 680

sc-8033 AF680
200 µg/ml
RMB2685.00

Insulin 抗体 (2D11-H5) Alexa Fluor® 790

sc-8033 AF790
200 µg/ml
RMB2685.00

É possivel utilizar o anticorpo Insulin Antibody (2D11-H5): sc-8033 para citometria de fluxo e imunofluorescência? Se sim, deve ser conjugado?

Asked by: Anonymous
Thank you for your question. We offer directly fluorescence-conjugated versions of Insulin Antibody (2D11-H5): sc-8033 that can be used in flow cytometry.
Answered by: Tech Support Europe
Date published: 2025-01-24

Can this antibody be used for western blot?

Asked by: MDAGirl
Thank you for your question. We recommend the antibody for IP, IF, IHC(P) and ELISA. Here is a publication for western blot : https://pubmed.ncbi.nlm.nih.gov/30386595/
Answered by: BlakeJ
Date published: 2024-06-06

Is this antibody and ready to ship overnight?

Asked by: Francis Kajumo
Thank you for your question. For stock and shipping please email customer care at [email protected]
Answered by: BlakeJ
Date published: 2023-03-22

Does this antibody capture proinsulin as well?

Asked by: Zoey111
Thank you for your question. Unfortunately, we do not have that information available for this insulin antibody.
Answered by: Technical Support
Date published: 2022-05-15

How to dilute this antibody? What volumes can i use to arrive at 1:200?

Asked by: anjan1983
Thank you for your question. This antibody is provided as 200µg in 1ml (1000µl), which means you have 1µg of antibody per 5µl of solution. So to make a 1:200 dilution, you need to take 1µg (5µl of solution) into 1ml of your diluent (milk/blocking reagent).
Answered by: Tech Service
Date published: 2019-08-30

what is the volume of primary antibody in the vial? sc-8033 200ug/ml?

Asked by: medha2001
Insulin Antibody (2D11-H5) is a mouse monoclonal IgG1 (kappa light chain) provided at 200 µg in a total volume of 1 ml PBS with 0.1 % gelatin and <0.1 % sodium azide.
Answered by: Tech Support Europe
Date published: 2019-08-30

Can this antibody be use to IF(frozen section)?

Asked by: Tienfen
Thank you for your question. This primary antibody is recommended and warrantied for IF (immunofluorescent staining of fresh or frozen tissues or cells fixed in methanol or acetone).
Answered by: Technical Support
Date published: 2019-04-22

Want to use antibody to distinguish proinsulin and mature insulin,what products are recommended?

Asked by: tinglan
Thank you for your question. Please contact our Technical Service Department and we can help recommend antibodies for the detection of proinsulin and mature insulin depending on what species and application(s) you need to use the antibodies in.
Answered by: Technical Support
Date published: 2024-04-25
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Rated 5 out of 5 by from GREAT FEATURES.I purchased this antibody two weeks ago. It worked very well on pancreatic section. I will buy this antibody if I need it later. Shirley
Date published: 2019-06-27
Rated 5 out of 5 by from Good Antibody for IHCGood signal on European seabass (Dicentrarchus labrax) fixed in Bouin's solution. Diluition 1:50
Date published: 2019-01-16
Rated 5 out of 5 by from Immunohistochemistry: excellent resultsIt works fine for Langerhans islets mouse and rat.
Date published: 2018-05-31
Rated 5 out of 5 by from Works great!Works in hES cells at 1:50 dilution for immunofluorescence
Date published: 2018-03-01
Rated 5 out of 5 by from Good anitibody for ELISAWe got 10 ug sample and used for ELISA. It gave a good result, with 1:300 dilution, and can detect ng/ml level human insulin.
Date published: 2018-02-20
Rated 5 out of 5 by from Easy to use on FFPE MouseWe received this as a sample. Before purchase a customer asked us to get a sample and work the antibody up. This is a straight forward, very easy, great looking antibody. Use a general protocol, sodium citrate pH6.0 dilution 1:100 - 1:250.
Date published: 2018-02-10
Rated 4 out of 5 by from Very goodgot a free sample and used to perform western blot analysis on mouse hippocampi. The detected band is very clear, and seems specific.
Date published: 2018-02-08
Rated 5 out of 5 by from Works beautifullyWorks beautifully to detect Insulin in murine tissues as confirmed Insulin KO mouse,1:600 dilution been used ,perfect result.
Date published: 2017-07-23
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Insulin 抗体 (2D11-H5) is rated 4.9 out of 5 by 22.
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