Date published: 2025-9-8
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IKKγ Antibody (F-12) - Western Blotting - Image 379865
IKKγ 抗体 (F-12): sc-166397. c4 (A), C2C12 (B), PC-3 (C), Jurkat (D), U-251-MG (E) 和 A-10 (F) 全细胞裂解液中 IKKγ 表达的 Western 印迹分析.
IKKγ 抗体 (F-12): sc-166397. c4 (A), C2C12 (B), PC-3 (C), Jurkat (D), U-251-MG (E) 和 A-10 (F) 全细胞裂解液中 IKKγ 表达的 Western 印迹分析.

IKKγ 抗体 (F-12): sc-166397

5.0(2)
寫評論提問

说明书
  • IKKγ抗体(F-12)是小鼠单克隆IgG1 κ, 在1篇文献中引用，规格为200 µg/ml
  • 针对全长IKKΓ，human起源
  • 推荐用于 mouse, rat 和 human 来源的IKKγ WB, IP 和 IF检测
  • 看 IKKγ (F-10): sc-166398，可查看有标记的 IKKγ 抗体，包括AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 和 790
  • m-IgG Fc BP-HRP和 1 BP-HRP">m-IgG1 BP-HRP是IKKγ Antibody (F-12) for WB applications. 的首选辅助检测试剂，这些试剂现与IKKγ Antibody (F-12) 打包提供（请参阅下面的订购信息）。

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関連項目

额外IKK抗体，包括IKK α, IKK β, IKK γ和IKK-epsilon

转录因子NFkB被抑制蛋白IkB以非活性形式保留在细胞质中。NFκB的激活需要IκB在特定的丝氨酸残基上被磷酸化，这会导致IκB的靶向降解。IκB激酶α（IKKα，以前称为CHUK）与IκB-α相互作用，并在丝氨酸32和36上特异性地磷酸化IκBα，这些位点是触发其降解的位点。IKKα似乎是响应促炎细胞因子而激活NFκB的关键。IKKα对IκB的磷酸化受到NFκB诱导激酶（NIK）的刺激，而NIK本身是响应TNF和IL-1而激活NFκB的中心调节因子。功能性的IKK复合物包含三个亚基，IKKα、IKKβ和IKKγ（也称为NEMO），每个亚基似乎都对IκB的磷酸化做出了重要贡献。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

订购信息

产品名称产品编号规格价格数量收藏夹

IKKγ 抗体 (F-12)

sc-166397
200 µg/ml
RMB2377.00

IKKγ (F-12): m-IgG Fc BP-HRP 套装

sc-540096
200 µg Ab; 10 µg BP
RMB2662.00

IKKγ (F-12): m-IgG1 BP-HRP 套装

sc-541901
200 µg Ab; 20 µg BP
RMB2662.00

I used to buy rabbit polyclonal NEMO antibody from your company for my IP and WB experiments. I just noted that you have stopped producing them. Do you still have some of these antibodies? or is the mouse monoclonal Ab any good for IP and WB? thank you

Asked by: Chung Lim
Thank you for your question. We no longer provide polyclonal antibodies. You can find all of our recommended monoclonal alternatives by following this link: https://www.scbt.com/scbt/browse/ikk-gamma-Antibodies/_/N-1w3ngw0. Our IKKγ Antibody (F-10): sc-166398 and IKKγ Antibody (B-3): sc-8032 are both recommended for IP and WB.
Answered by: SCBT Heidelberg Support
Date published: 2018-12-29

What blocking solution should I use with IKKγ (F-12): sc-166397 monoclonal antibody for Western blot?

Asked by: cjMara
Thank you for your question. We recommend blocking with Blotto (either TBS Blotto A: sc-2333 or TBS Blotto B: sc-2335). Our Western blot protocol can be found here: https://www.scbt.com/scbt/resources/protocols/western-immuno-blotting
Answered by: Technical Support
Date published: 2017-03-01
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Rated 5 out of 5 by from Positive WB data with Jurkat whole cell lysatePositive WB data with Jurkat whole cell lysate. -SCBT QC
Date published: 2015-03-06
Rated 5 out of 5 by from Great WB data in human and mouse transfectedGreat WB data in human and mouse transfected lysates. -SCBT QC
Date published: 2013-07-30
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IKKγ 抗体 (F-12) is rated 5.0 out of 5 by 2.
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