Date published: 2025-9-8

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IKKγ 抗体 (B-3): sc-8032

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说明书
  • IKKγ抗体(B-3)是小鼠单克隆IgG1 κ, 在42篇文献中引用,规格为200 µg/ml
  • 免疫1-419氨基酸,对应于human来源IKKγ全长
  • 抗-IKK gamma 抗体 (B-3) 推荐用于 WB, IP, IF 和 ELISA,检测mouse, rat 和human 来源的 IKKγ
  • 抗IKK gamma抗体(B-3)可与琼脂糖结合用于IP;与HRP结合用于WB、IHC(P)和ELISA;与藻红蛋白FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取IKKγ (B-3): sc-8032免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • 1 BP-HRP">m-IgG1 BP-HRP是IKKγ Antibody (B-3) 适用于 WB 应用。 的首选辅助检测试剂。该试剂现与IKKγ Antibody (B-3) 打包提供(请参阅下面的订购信息)。

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関連項目

IKKγ抗体(B-3)是一种小鼠单克隆IgG1 kappa轻链抗体,专门用于检测小鼠、大鼠和人类等多种物种中的IKKγ(也称为NEMO(NF-κB Essential Modulator))。IKKγ单克隆抗体(B-3)靶向全长人类IKKγ表位,跨越1-419个氨基酸,确保在各种应用中(如蛋白质印迹(WB)、免疫沉淀(IP)、免疫荧光(IF)和酶联免疫吸附测定(ELISA))具有全面的识别能力和可靠的性能。IKKγ在NF-κB信号传导通路中起着关键作用,是IKK复合物的重要调节亚基,对IκB蛋白的磷酸化降解至关重要。这个过程使转录因子NF-κB能够转移到细胞核中,激活与免疫反应、炎症和细胞存活相关的基因,因此IKKγ成为研究这些基本生物学机制的重要靶标。IKKγ的功能对于理解细胞如何响应促炎细胞因子和应激信号至关重要,凸显了其在各种生理和病理环境中的重要性。IKKγ单克隆抗体(B-3)有非结合型和多种结合型,包括辣根过氧化物酶(HRP)、藻红蛋白(PE)、异硫氰酸荧光素(FITC)和各种Alexa Fluor®结合物,为研究人员提供了满足其特定实验需求的多功能选择。IKKγ单克隆抗体(B-3)推荐用于研究NF-κB途径的复杂细节及其在癌症、自身免疫性疾病和慢性炎症等疾病中的影响。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

IKKγ 抗体 (B-3) 参考文献:

  1. IKK调控和人类遗传学。  |  Courtois, G. and Israël, A. 2011. Curr Top Microbiol Immunol. 349: 73-95. PMID: 20845108
  2. GSK-3β 通过 IKKγ/NEMO 控制 NF-kappaB 的活性  |  Medunjanin, S., et al. 2016. Sci Rep. 6: 38553. PMID: 27929056
  3. IKKγ/NEMO定位到多囊泡体中。  |  Wackernagel, LM., et al. 2022. Int J Mol Sci. 23: PMID: 35743220
  4. Rel/NF-kappa B/I kappa B 家族:关联与分离的亲密故事。  |  Verma, IM., et al. 1995. Genes Dev. 9: 2723-35. PMID: 7590248
  5. NF-kappa B:家庭价值观的一课。  |  Thanos, D. and Maniatis, T. 1995. Cell. 80: 529-32. PMID: 7867060
  6. CHUK是螺旋-环-螺旋和亮氨酸拉链相互作用蛋白家族的新成员,含有丝氨酸-苏氨酸激酶催化结构域。  |  Connelly, MA. and Marcu, KB. 1995. Cell Mol Biol Res. 41: 537-49. PMID: 8777433
  7. 参与 TNF, CD95 和 IL-1 诱导 NF-kappaB 的 MAP3K 相关激酶。  |  Malinin, NL., et al. 1997. Nature. 385: 540-4. PMID: 9020361
  8. IkappaB 激酶的鉴定和表征。  |  Régnier, CH., et al. 1997. Cell. 90: 373-83. PMID: 9244310
  9. 一种细胞因子反应型 IkappaB 激酶,可激活转录因子 NF-kappaB。  |  DiDonato, JA., et al. 1997. Nature. 388: 548-54. PMID: 9252186
  10. 肿瘤坏死因子(TNF)介导的激酶级联:核因子-kappaB 和 c-jun N 端激酶(JNK/SAPK)通路在 TNF 受体相关因子 2 上的分叉。  |  Song, HY., et al. 1997. Proc Natl Acad Sci U S A. 94: 9792-6. PMID: 9275204
  11. IkappaB 激酶复合物(IKK)包含两个激酶亚基 IKKalpha 和 IKKbeta,它们是 IkappaB 磷酸化和 NF-kappaB 激活所必需的。  |  Zandi, E., et al. 1997. Cell. 91: 243-52. PMID: 9346241
  12. NEMO 的补全克隆,NEMO 是 IkappaB 激酶复合物的一个组成部分,对 NF-kappaB 激活至关重要。  |  Yamaoka, S., et al. 1998. Cell. 93: 1231-40. PMID: 9657155

订购信息

产品名称产品编号规格价格数量收藏夹

IKKγ 抗体 (B-3)

sc-8032
200 µg/ml
RMB2377.00

IKKγ (B-3): m-IgG1 BP-HRP 套装

sc-531630
200 µg Ab; 20 µg BP
RMB2662.00

IKKγ 抗体 (B-3) AC

sc-8032 AC
500 µg/ml, 25% agarose
RMB3129.00

IKKγ 抗体 (B-3) HRP

sc-8032 HRP
200 µg/ml
RMB2377.00

IKKγ 抗体 (B-3) FITC

sc-8032 FITC
200 µg/ml
RMB2482.00

IKKγ 抗体 (B-3) PE

sc-8032 PE
200 µg/ml
RMB2580.00

IKKγ 抗体 (B-3) Alexa Fluor® 488

sc-8032 AF488
200 µg/ml
RMB2685.00

IKKγ 抗体 (B-3) Alexa Fluor® 546

sc-8032 AF546
200 µg/ml
RMB2685.00

IKKγ 抗体 (B-3) Alexa Fluor® 594

sc-8032 AF594
200 µg/ml
RMB2685.00

IKKγ 抗体 (B-3) Alexa Fluor® 647

sc-8032 AF647
200 µg/ml
RMB2685.00

IKKγ 抗体 (B-3) Alexa Fluor® 680

sc-8032 AF680
200 µg/ml
RMB2685.00

IKKγ 抗体 (B-3) Alexa Fluor® 790

sc-8032 AF790
200 µg/ml
RMB2685.00

What is the dilution of SC-8032 for WB?

Asked by: zheny
Thank you for your question. We recommend a starting dilution of 1:200 (1:100-1:1,000) for Western blotting.
Answered by: Technical Support
Date published: 2019-07-13

I am using the LiCor/Odyssey system. Which secondary antibody do you recommend using with IKKy Antibody (B-3): sc-8032?

Asked by: Trav11
We recommend using one of our our exclusive Mouse IgG Binding Proteins (m-IgGκ) conjugated to CFL 680 or CFL 790. They are suitable for use with Near-Infrared (NIR) detection systems, such as LI-COR/Odyssey and other comparable systems. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Answered by: Tech Service 8
Date published: 2017-01-30
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Rated 5 out of 5 by from Well characterized IKK gamma ab for WB, IF & IHCProduced more than a 2 dozen citations in the primarey literature on human, mouse and rat samples in a wide variety of applications.
Date published: 2017-01-06
Rated 5 out of 5 by from goodThis antibody is very good for WB in 293 and other human cells
Date published: 2016-12-05
Rated 5 out of 5 by from greatthis antibody is very good for Western blot in 293 cells and 549.
Date published: 2016-12-02
Rated 4 out of 5 by from IF images were goodIF images were good. Worked well on mouse cells.
Date published: 2015-10-12
Rated 4 out of 5 by from Used in IP in mouse cellsUsed in IP in mouse cells.
Date published: 2015-07-29
Rated 5 out of 5 by from WB Published with this antibody using humanWB Published with this antibody using human HEK293T. -SCBT Publication Review
Date published: 2015-05-14
Rated 5 out of 5 by from Exceptional immunofluorescence cytoplasmicExceptional immunofluorescence cytoplasmic staining in formalin-fixed HepG2 cells. -SCBT QC
Date published: 2015-04-13
Rated 5 out of 5 by from Great antibody for human IPGreat antibody for human IP, published using t-cells. -SCBT Publication Review
Date published: 2015-03-18
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IKKγ 抗体 (B-3) is rated 4.8 out of 5 by 9.
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