Date published: 2025-9-7
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IKKγ Antibody (F-10) - Western Blotting - Image 379864
IKKγ 抗体 (F-10): sc-166398. PC-3 (A), U-251-MG (B), c4 (C), C2C12 (D), A-10 (E) 和 C6 (F) 全细胞裂解液中 IKKγ 表达的 Western 印迹分析.
IKKγ 抗体 (F-10): sc-166398. PC-3 (A), U-251-MG (B), c4 (C), C2C12 (D), A-10 (E) 和 C6 (F) 全细胞裂解液中 IKKγ 表达的 Western 印迹分析.

IKKγ 抗体 (F-10): sc-166398

4.4(5)
寫評論提問

说明书
  • IKK gamma 抗体 F-10 是小鼠单克隆 IgG1 κ，IKKγ抗体, 在5篇文献中引用，规格为200 µg/ml
  • 针对全长IKKΓ，human起源
  • IKK gamma 抗体 (F-10) 推荐用于 WB, IP 和 IF，检测mouse, rat 和human 来源的 IKKγ
  • 抗IKK gamma抗体（F-10）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取IKKγ (F-10): sc-166398的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP、 1 BP-HRP">m-IgG1 BP-HRP和m-IgGκ BP-HRP是IKKγ Antibody (F-10) 适用于 WB 应用。 的首选辅助检测试剂。这些试剂现与IKKγ Antibody (F-10) 打包提供（请参阅下面的订购信息）。

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描述
基因信息
说明书与实验方案
研究信息

関連項目

额外IKK抗体，包括IKK α, IKK β, IKK γ和IKK-epsilon

IKKγ抗体（F-10）是一种IgG1 κ型小鼠单克隆IKKγ抗体（也称为NEMO抗体），该抗体可通过WB, IP和IF检测小鼠、大鼠和人类来源的IKKγ蛋白。IKKγ抗体（F-10）既可作为非偶联形式的抗IKKγ抗体，也可作为多种偶联形式的抗IKKγ抗体，包括琼脂糖、辣根过氧化物酶（HRP）、藻红蛋白（PE）、异硫氰酸荧光素（FITC）和多种Alexa Fluor®偶联物。转录因子NFκB以非活性形式被抑制蛋白IκB保留在细胞质中。NFκB的激活需要IκB在特定的丝氨酸残基上被磷酸化，这会导致IκB的靶向降解。IκB激酶α（IKKα，以前称为CHUK）与IκB-α相互作用，并在丝氨酸32和36上特异性地磷酸化IκBα，这些位点是触发其降解的位点。IKKα似乎是响应促炎细胞因子而激活NFκB的关键。IKKα对IκB的磷酸化受到NFκB诱导激酶（NIK）的刺激，而NIK本身是响应TNF和IL-1而激活NFκB的中心调节因子。功能性的IKK复合物包含三个亚基，IKKα、IKKβ和IKKγ（也称为NEMO），每个亚基似乎都对IκB的磷酸化做出了重要贡献。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

订购信息

产品名称产品编号规格价格数量收藏夹

IKKγ 抗体 (F-10)

sc-166398
200 µg/ml
RMB2377.00

IKKγ (F-10): m-IgG Fc BP-HRP 套装

sc-528955
200 µg Ab; 10 µg BP
RMB2662.00

IKKγ (F-10): m-IgGκ BP-HRP 套装

sc-521590
200 µg Ab, 40 µg BP
RMB2662.00

IKKγ (F-10): m-IgG1 BP-HRP 套装

sc-543234
200 µg Ab; 20 µg BP
RMB2662.00

IKKγ 抗体 (F-10) AC

sc-166398 AC
500 µg/ml, 25% agarose
RMB3129.00

IKKγ 抗体 (F-10) HRP

sc-166398 HRP
200 µg/ml
RMB2377.00

IKKγ 抗体 (F-10) FITC

sc-166398 FITC
200 µg/ml
RMB2482.00

IKKγ 抗体 (F-10) PE

sc-166398 PE
200 µg/ml
RMB2580.00

IKKγ 抗体 (F-10) Alexa Fluor® 488

sc-166398 AF488
200 µg/ml
RMB2685.00

IKKγ 抗体 (F-10) Alexa Fluor® 546

sc-166398 AF546
200 µg/ml
RMB2685.00

IKKγ 抗体 (F-10) Alexa Fluor® 594

sc-166398 AF594
200 µg/ml
RMB2685.00

IKKγ 抗体 (F-10) Alexa Fluor® 647

sc-166398 AF647
200 µg/ml
RMB2685.00

IKKγ 抗体 (F-10) Alexa Fluor® 680

sc-166398 AF680
200 µg/ml
RMB2685.00

IKKγ 抗体 (F-10) Alexa Fluor® 790

sc-166398 AF790
200 µg/ml
RMB2685.00

Can IKKγ (F-10): sc-166398 monoclonal antibody be used in IF or IHC with mouse tissue or cells? Will there be non-specific staining?

Asked by: DefinitelyNotMatt
Thank you for your inquiry. The use of mouse monoclonal antibodies with mouse samples is very common and typically poses no problem. To eliminate any potential cross-reactivity of an anti-mouse secondary detection reagent, IKKγ (F-10): sc-166398 is available in a variety of direct conjugations, such as HRP, PE, FITC and AlexaFluors.
Answered by: Technical Support
Date published: 2017-02-24
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Rated 5 out of 5 by from Good antibody for detecting endogenous NEMOExcellent antibody for detecting endogenous NEMO. It is also good for IP.
Date published: 2017-09-22
Rated 2 out of 5 by from MW band wrong size.I previously used a Santa Cruz anti-IKKg antibody and liked it very much. As it was discontinued, I tried this one instead. There is a faint band that is almost the right size (a bit low), and several strong bands of completely the wrong size.
Date published: 2017-03-07
Rated 5 out of 5 by from Works wellantibody works well in WB of lysis of MEFs/BMDM cells
Date published: 2016-12-02
Rated 5 out of 5 by from Highly potent and very specific antibodyThis monoclonal antibody was independently tested in-house by Kinexus Bioinformatics Corporation and validated for use for Western blotting. There was very strong detection of the ~48 kDa target protein in mouse tissues (brain/testes/thymus/muscle mix). No major cross-reactivities were observed at 2 µg/ml antibody concentration with off-target proteins in the mouse tissues tested.
Date published: 2016-11-10
Rated 5 out of 5 by from Fantastic WB data in mouse and human transfectedFantastic WB data in mouse and human transfected lysates. -SCBT QC
Date published: 2013-02-02
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IKKγ 抗体 (F-10) is rated 4.4 out of 5 by 5.
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