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IFN-γ/Interferon gamma 抗体 (F3): sc-59993

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  • IFN-γ/Interferon gamma 抗体 F3 是大鼠单克隆 IgG2a, 在6篇文献中引用,提供 100 µg/ml
  • 免疫mouse物种的IFN-γ
  • 推荐用于 mouse 来源的IFN-γ WB 和 IP检测
  • 目前,我们还没有完成IFN-γ/Interferon gamma 抗体 (F3)的首选二抗检测试剂的鉴定。这项工作正在进行中。
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IFN-γ 抗体(F3)是一种大鼠单克隆 IgG2a 抗体,可通过免疫印迹(WB)和免疫沉淀(IP)检测小鼠样本中的干扰素(IFN)-γ。IFN-γ 在免疫反应中起着至关重要的作用,是一种由活化的 CD4+/CD8+ 淋巴细胞和自然杀伤细胞产生的强效抗病毒和抗寄生虫药物。IFN-γ 可调节 T 细胞的生长和分化,同时抑制 B 细胞的增殖,从而形成适应性免疫反应。IFN-γ 的活性形式是一种同源二聚体,每个亚基由六个螺旋组成,这种二聚体结构通过螺旋界面上的非共价相互作用而稳定。IFN-γ 的前体由 166 个氨基酸组成,其中包括 23 个氨基酸的分泌序列,其功能通过可变糖基化进一步增强,从而形成多种形式,在非变性条件下可以二聚体和四聚体形式存在。抗 IFN-γ 抗体(F3)是研究人员在小鼠模型中研究免疫反应和细胞因子信号转导复杂机制的重要工具。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

IFN-γ/Interferon gamma 抗体 (F3) 参考文献:

  1. 肿瘤发生中IFN-γ信号传导途径的组成部分。  |  Blanck, G. 2002. Arch Immunol Ther Exp (Warsz). 50: 151-8. PMID: 12098930
  2. IFN-γ及其受体亚基IFNGR1被招募到IFN-γ激活基因启动子位点的IFN-γ激活序列元件中:IFNGR1转激活活性的证据。  |  Ahmed, CM. and Johnson, HM. 2006. J Immunol. 177: 315-21. PMID: 16785527
  3. 一种新型内化基序可调节人类IFN-γ R1的内吞作用。  |  Yancoski, J., et al. 2012. J Leukoc Biol. 92: 301-8. PMID: 22595141
  4. IFN-γ信号通过调节黑素体成熟来维持皮肤色素沉着平衡。  |  Natarajan, VT., et al. 2014. Proc Natl Acad Sci U S A. 111: 2301-6. PMID: 24474804
  5. IFN-γ AU-rich元件的去除可促进小鼠慢性IFN-γ表达和自身免疫。  |  Hodge, DL., et al. 2014. J Autoimmun. 53: 33-45. PMID: 24583068
  6. 宫颈癌组织中IFN-γ基因的甲基化模式。  |  Ma, D., et al. 2014. Sci Rep. 4: 6331. PMID: 25208560
  7. 毛蕊花糖苷的抗病毒作用及其潜在的IFN-γ诱导作用。  |  Song, X., et al. 2016. Food Funct. 7: 3017-30. PMID: 27326537
  8. 组胺受体拮抗剂抑制小鼠脾细胞IFN-γ的产生。  |  Kamei, M., et al. 2018. Int J Mol Sci. 19: PMID: 30562962
  9. 体外SARS-CoV-2刺激后,IFN-γ+细胞反应和IFN-γ释放一致。  |  Vallejo, A., et al. 2021. Eur J Clin Invest. 51: e13636. PMID: 34164811
  10. 巨噬细胞中IFN-γ诱导的CIITA涉及JAK和JNK激活的STAT1。  |  Tur, J., et al. 2021. Immunobiology. 226: 152114. PMID: 34303919

订购信息

产品名称产品编号规格价格数量收藏夹

IFN-γ/Interferon gamma 抗体 (F3)

sc-59993
100 µg/ml
CNY2422.00

What region of the antigen does this antibody bind to? I'm looking at sc373727 which is specific to an epitope mapping between amino acids 25-50 at the N-terminus of human IFNg. Depends on where sc59993 binds to, I may be able to use these as FRET pair.

Asked by: ChuR
Thank you for your question. It would be helpful if you could call 800-457-3801, allowing for a more interactive discussion of this and other related questions.
Answered by: Technical Service
Date published: 2022-03-02

What blocking solution should I use with IFN-γ (F3): sc-59993 monoclonal antibody for Western blot?

Asked by: Cweed
Thank you for your question. We recommend blocking with Blotto (either TBS Blotto A: sc-2333 or TBS Blotto B: sc-2335) Our Western blot protocol can be found here: https://www.scbt.com/scbt/resources/protocols/western-immuno-blotting
Answered by: Technical Support
Date published: 2017-04-05
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Rated 3 out of 5 by MS from Published data for WB in mouse melanoma cellPublished data for WB in mouse melanoma cell lines; B16F10 and B16LNAD, human embryo kidney 293T cells. -SCBT Publication Review
Date published: 2015-02-25
Rated 2 out of 5 by FL from Positive band detected in WB for mouse recombinantPositive band detected in WB for mouse recombinant IFN- . -SCBT QC
Date published: 2013-12-22
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IFN-γ/Interferon gamma 抗体 (F3) is rated 2.5 out of 5 by 2.
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