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IFN-γ/Interferon gamma Antibody (G-23) - Western Blotting - Image 2675
IFN-γ (G-23): sc-8423. 人重组 IFN-γ 的 Western 印迹分析.
IFN-γ/Interferon gamma Antibody (G-23) - Western Blotting - Image 2675
IFN-γ (G-23): sc-8423. 人重组 IFN-γ 的 Western 印迹分析.
IFN-γ/Interferon gamma Antibody (G-23) - Flow Cytometry - Image 5795
IFN-γ (G-23) FITC: sc-8423 FITC. PMA/ionomycin 刺激的全人类血液的 FCM 分析, 用 CD69 (FN50) PE: sc-18880 PE 染色. 细胞固定并透化, 然后用 IFN-γ (G-23) FITC: sc-8423 FITC 染色. 象限标记根据同型对照, 正常小鼠 IgG1: sc-2855 和 sc-2866 设置.
IFN-γ (G-23): sc-8423. 人重组 IFN-γ 的 Western 印迹分析.

IFN-γ/Interferon gamma 抗体 (G-23): sc-8423

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寫評論提問

说明书
  • IFN-γ/Interferon gamma抗体(G-23)是小鼠单克隆IgG1 κ, 在13篇文献中引用，规格为200 µg/ml
  • 针对代表human来源的成熟干扰素-γ（IFN-γ）的氨基酸22-166
  • IFN-γ/Interferon gamma 抗体 (G-23) 推荐用于WB, IP, IF, FCM 和 ELISA，检测human 来源的 precursor and mature IFN-γ
  • 抗IFN-γ/Interferon gamma抗体（G-23）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取IFN-γ/Interferon gamma (G-23): sc-8423的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP和 1 BP-HRP">m-IgG1 BP-HRP是IFN-γ/Interferon gamma Antibody (G-23) for WB applications. 的首选辅助检测试剂，这些试剂现与IFN-γ/Interferon gamma Antibody (G-23) 打包提供（请参阅下面的订购信息）。

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描述
基因信息
说明书与实验方案
研究信息

関連項目

额外IFN抗体，包括IFN-α, IFN-α 1, IFN-α 13, IFN-α 2, IFN-β, IFN-γ和IFN-ω

IFN-γ抗体（G-23）是一种鼠单克隆IgG1 kappa轻链抗体，可通过蛋白质印迹（WB）、免疫沉淀（IP）、免疫荧光（IF）、流式细胞术（FCM）和酶联免疫吸附测定（ELISA）检测人源干扰素γ（IFN-γ）。IFN-γ抗体（G-23）有非结合型和多种结合型，包括琼脂糖、辣根过氧化物酶（HRP）、藻红蛋白（PE）、异硫氰酸荧光素（FITC）和多种Alexa Fluor®结合物。γ干扰素在免疫反应中起着至关重要的作用，是一种抗病毒和抗寄生虫的物质，主要由CD4+/CD8+淋巴细胞和自然杀伤细胞在抗原、促有丝分裂素或同种抗原的激活下产生。IFN-γ可调节T细胞的生长和分化，同时抑制B细胞的增殖，从而维持免疫反应的平衡。IFN-γ的活性形式是一种同源二聚体，每个亚基由六个螺旋组成，这种二聚体结构通过螺旋界面上的非共价相互作用而稳定。IFN-γ的前体由166个氨基酸组成，其中包括一个23个氨基酸的分泌序列，IFN-γ的功能会受到多种翻译后修饰的影响，包括糖基化，导致在非变性条件下存在多种形式，如二聚体和四聚体。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

IFN-γ/Interferon gamma 抗体 (G-23) 参考文献:

  1. γ干扰素的抗病毒反应。  |  Costa-Pereira, AP., et al. 2002. J Virol. 76: 9060-8. PMID: 12186889
  2. 组蛋白去乙酰化酶1/MSin3A会破坏γ干扰素诱导的CIITA功能和主要组织相容性复合体II类增强体的形成。  |  Zika, E., et al. 2003. Mol Cell Biol. 23: 3091-102. PMID: 12697811
  3. 干扰素-γ：信号, 机制和功能概述。  |  Schroder, K., et al. 2004. J Leukoc Biol. 75: 163-89. PMID: 14525967
  4. γ干扰素激活多形核中性粒细胞功能  |  Ellis, TN. and Beaman, BL. 2004. Immunology. 112: 2-12. PMID: 15096178
  5. 激活干扰素γ刺激基因的一个子集需要卡巴激酶抑制剂。  |  Sizemore, N., et al. 2004. Proc Natl Acad Sci U S A. 101: 7994-8. PMID: 15148408
  6. 在角质形成细胞中，干扰素-γ 依赖性 MEKK4 通过 Pyk2 产生的酪氨酸磷酸化受 annexin II 和 SHP2 的调控。  |  Halfter, UM., et al. 2005. Biochem J. 388: 17-28. PMID: 15601262
  7. 神经元中IFN-γ/STAT1信号传导的独特方面。  |  Clark, DN., et al. 2022. Immunol Rev. 311: 187-204. PMID: 35656941
  8. 人类 IRF1 控制巨噬细胞对分枝杆菌的 IFN-γ 免疫。  |  Rosain, J., et al. 2023. Cell. 186: 621-645.e33. PMID: 36736301
  9. SEPTIN2抑制IFN-γ非依赖性促炎性巨噬细胞活化途径。  |  Fu, B., et al. 2023. Nat Commun. 14: 7441. PMID: 37978190
  10. 干扰素-γ 在免疫细胞调节中的作用。  |  Young, HA. and Hardy, KJ. 1995. J Leukoc Biol. 58: 373-81. PMID: 7561512
  11. 白细胞介素-18 概述：不仅仅是一种干扰素-γ 诱导因子。  |  Dinarello, CA., et al. 1998. J Leukoc Biol. 63: 658-64. PMID: 9620656
  12. IL-12 和 IL-18 对干扰素-γ 生成的调节。  |  Okamura, H., et al. 1998. Curr Opin Immunol. 10: 259-64. PMID: 9638361

订购信息

产品名称产品编号规格价格数量收藏夹

IFN-γ/Interferon gamma 抗体 (G-23)

sc-8423
200 µg/ml
RMB2377.00

IFN-γ/Interferon gamma (G-23): m-IgG Fc BP-HRP 套装

sc-526523
200 µg Ab; 10 µg BP
RMB2662.00

IFN-γ/Interferon gamma (G-23): m-IgG1 BP-HRP 套装

sc-531896
200 µg Ab; 20 µg BP
RMB2662.00

IFN-γ/Interferon gamma 抗体 (G-23) AC

sc-8423 AC
500 µg/ml, 25% agarose
RMB3129.00

IFN-γ/Interferon gamma 抗体 (G-23) HRP

sc-8423 HRP
200 µg/ml
RMB2377.00

IFN-γ/Interferon gamma 抗体 (G-23) FITC

sc-8423 FITC
200 µg/ml
RMB2482.00

IFN-γ/Interferon gamma 抗体 (G-23) PE

sc-8423 PE
200 µg/ml
RMB2580.00

IFN-γ/Interferon gamma 抗体 (G-23) Alexa Fluor® 488

sc-8423 AF488
200 µg/ml
RMB2685.00

IFN-γ/Interferon gamma 抗体 (G-23) Alexa Fluor® 546

sc-8423 AF546
200 µg/ml
RMB2685.00

IFN-γ/Interferon gamma 抗体 (G-23) Alexa Fluor® 594

sc-8423 AF594
200 µg/ml
RMB2685.00

IFN-γ/Interferon gamma 抗体 (G-23) Alexa Fluor® 647

sc-8423 AF647
200 µg/ml
RMB2685.00

IFN-γ/Interferon gamma 抗体 (G-23) Alexa Fluor® 680

sc-8423 AF680
200 µg/ml
RMB2685.00

IFN-γ/Interferon gamma 抗体 (G-23) Alexa Fluor® 790

sc-8423 AF790
200 µg/ml
RMB2685.00

Dear Sirs, I am interested in antibody pair against human IFNγ, suitable for ELISA. Detection antibody shoul be HRP labeled or unlabeled. What could you recommend me? Thanks in advance. Kind regards, Danica

Asked by: Danica
Thank you for your question. It would be helpful if you could call 800-457-3801, allowing for a more interactive discussion of this and other related questions.
Answered by: Technical Service
Date published: 2022-04-29

Can IFN-γ (G-23): sc-8423 monoclonal antibody be used in IF or IHC with mouse tissue or cells? Will there be non-specific staining?

Asked by: Professor Griffin
Thank you for your inquiry. The use of mouse monoclonal antibodies with mouse samples is very common and typically poses no problem. To eliminate any potential cross-reactivity of an anti-mouse secondary detection reagent, IFN-γ (G-23): sc-8423 is available in a variety of direct conjugations, such as HRP, PE, FITC and AlexaFluors.
Answered by: Technical Support
Date published: 2017-02-24
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Rated 3 out of 5 by from Good signal by WBI compared this antibody with an alternative, and it worked just fine (WB).
Date published: 2021-05-20
Rated 5 out of 5 by from Great antibody for WB!Highly recommend! Works great for WB application probing in vivo mouse tissue lysates at 1:200.
Date published: 2018-03-23
Rated 4 out of 5 by from Good antibodyWorked well with western blotting at 1:500 dilution.
Date published: 2017-09-27
Rated 5 out of 5 by from Strong pulldown in IP in human 293Strong pulldown in IP in human 293, T98G cells. -SCBT Publication Review
Date published: 2015-05-07
Rated 5 out of 5 by from Good publishable data in IHC in human lung tissueGood publishable data in IHC in human lung tissue. -SCBT Publication Review
Date published: 2015-02-14
Rated 5 out of 5 by from Good specificity in WB in human dermal lesionGood specificity in WB in human dermal lesion tissue. -SCBT Publication Review
Date published: 2015-02-05
Rated 5 out of 5 by from Published in IP and WB in human HEK293 cellsPublished in IP and WB in human HEK293 cells. -SCBT Publication Review
Date published: 2015-02-05
Rated 5 out of 5 by from Produced nice FCM data for PMA/ionomycinProduced nice FCM data for PMA/ionomycin stimulated whole human blood, stained with CD69 PE. -SCBT QC
Date published: 2014-05-14
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IFN-γ/Interferon gamma 抗体 (G-23) is rated 4.6 out of 5 by 8.
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