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IFN-γ/Interferon gamma Antibody (E-10) - Western Blotting - Image 146558 — IFN-γ (E-10): sc-373727. 人重组 IFN-γ 融合蛋白的 Western 印迹分析.

IFN-γ (E-10): sc-373727. 人重组 IFN-γ 融合蛋白的 Western 印迹分析.

IFN-γ/Interferon gamma 抗体 (E-10): sc-373727

Name: IFN-&gamma;/Interferon gamma抗体 (E-10)
Brand: SCBIO
SKU: sc-373727
Price: 2377.0 CNY
Availability: InStock

3.5(6)

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说明书

IFN-γ/Interferon gamma 抗体 E-10 是小鼠单克隆 IgG_2a κ，IFN-γ/Interferon gamma抗体, 在15篇文献中引用，规格为200 µg/ml
特异性抗原位于human物种的IFN-γ的N-terminus的氨基酸25-50之间
IFN-γ/Interferon gamma 抗体 (E-10) 推荐用于WB, IP, IF 和 ELISA，检测human 来源的 precursor and mature IFN-γ
抗IFN-γ/Interferon gamma抗体（E-10）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
还可偶联Alexa Fluor^® 488, Alexa Fluor^® 546, Alexa Fluor^® 594 和 Alexa Fluor^® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
还可偶联Alexa Fluor^® 680 和 Alexa Fluor^® 790，用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR^®/Odyssey^®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
关于如何获取IFN-γ/Interferon gamma (E-10): sc-373727的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
m-IgGκ BP-HRP是IFN-γ/Interferon gamma Antibody (E-10) 适用于 WB 应用。的首选辅助检测试剂。该试剂现可与IFN-γ/Interferon gamma Antibody (E-10) 搭配使用（请参阅下面的订购信息）。有关其它 m-IgGκ BP 结合物，请参阅我们完整的小鼠 IgG 结合蛋白列表。

查看产品引用文献(14)

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関連項目

附加IFN抗体，包括IFN-α, IFN-α 1, IFN-α 13, IFN-α 2, IFN-β, IFN-γ和IFN-ω

IFN-γ抗体（E-10）是一种IgG2a κ小鼠单克隆IFN-gamma抗体（也称为IFNG抗体、干扰素gamma抗体、免疫干扰素抗体、IFN gamma抗体或干扰素-γ抗体），可通过WB、IP、IF和ELISA检测人源IFN-gamma蛋白。 IFN-γ抗体（E-10）既可作为非偶联抗IFN-gamma抗体形式，也可作为多种偶联形式的抗IFN-gamma抗体，包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®偶联物。干扰素(IFN)-γ是一种由CD4+/CD8+淋巴细胞和自然杀伤细胞产生的抗病毒和抗寄生虫剂，这些细胞通过抗原、有丝分裂原或同种异型抗原进行激活。 IFN-γ的产生可调节T细胞的生长和分化，并抑制B细胞的生长。 IFN-γ的合成可通过IL-2、FGF和EGF诱导。 IFN-γ的活性形式是同型二聚体，每个亚基包含六个螺旋。人IFN-γ的二聚体结构通过螺旋界面的非共价相互作用而稳定。 IFN-γ翻译前体是166个氨基酸，包括23个氨基酸的分泌序列。由于可变的糖基化和非变性条件，存在多种形式，包括二聚体和四聚体。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor^® 是Molecular Probes Inc., OR., USA的商标

LI-COR^®和 Odyssey^® 是LI-COR Biosciences的注册商标

订购信息

产品名称	产品编号	规格	价格	数量	收藏夹
IFN-γ/Interferon gamma 抗体 (E-10)	sc-373727	200 µg/ml	RMB2377.00
IFN-γ/Interferon gamma (E-10): m-IgGκ BP-HRP 套装	sc-522473	200 µg Ab, 40 µg BP	RMB2662.00
IFN-γ/Interferon gamma 抗体 (E-10) AC	sc-373727 AC	500 µg/ml, 25% agarose	RMB3129.00
IFN-γ/Interferon gamma 抗体 (E-10) HRP	sc-373727 HRP	200 µg/ml	RMB2377.00
IFN-γ/Interferon gamma 抗体 (E-10) FITC	sc-373727 FITC	200 µg/ml	RMB2482.00
IFN-γ/Interferon gamma 抗体 (E-10) PE	sc-373727 PE	200 µg/ml	RMB2580.00
IFN-γ/Interferon gamma 抗体 (E-10) Alexa Fluor^® 488	sc-373727 AF488	200 µg/ml	RMB2685.00
IFN-γ/Interferon gamma 抗体 (E-10) Alexa Fluor^® 546	sc-373727 AF546	200 µg/ml	RMB2685.00
IFN-γ/Interferon gamma 抗体 (E-10) Alexa Fluor^® 594	sc-373727 AF594	200 µg/ml	RMB2685.00
IFN-γ/Interferon gamma 抗体 (E-10) Alexa Fluor^® 647	sc-373727 AF647	200 µg/ml	RMB2685.00
IFN-γ/Interferon gamma 抗体 (E-10) Alexa Fluor^® 680	sc-373727 AF680	200 µg/ml	RMB2685.00
IFN-γ/Interferon gamma 抗体 (E-10) Alexa Fluor^® 790	sc-373727 AF790	200 µg/ml	RMB2685.00
IFN-γ/Interferon gamma (E-10) 中和勝肽	sc-373727 P	100 µg/0.5 ml	RMB511.00

What application is the blocking peptide sc-373727 P appropriate for?

Asked by: Trav11

Thank you for your question. The blocking peptide is intended for use as a negative control, by pre-adsorbing the mouse monoclonal antibody against the antigen. For full protocol details, please contact our Technical Services Department or view our online protocol here: https://www.scbt.com/scbt/resources/protocols/peptide-neutralization

Answered by: Technical Support

Date published: 2017-02-28

Rated 3 out of 5 by MuGu from better results with sc-8423pref. the sc-8423 product over this when working with mouse samples.

Date published: 2018-03-23

Rated 1 out of 5 by Mauro from Holy (and expensive) waterTried the free IFNgamma (E-10) in Western blot (1:100 as suggested by datasheet) with olfactory mesenchymal stem cells (oMSC) and mouse cortex protein extracts to check for signal in these two models. Not a single band was detected. Everything was done properly. I have tested 8 (eight!) mouse monoclonal antibodies from Santa Cruz and not a single one gave any band except one that gave an aspecific signal (I would like to upload a picture, but it is not allowing me). I will not loose more time with them and I strongly advice you to do the same. Good luck, fellow researchers.

Date published: 2017-11-17

Rated 3 out of 5 by Tsen from Good antibodyWorked well with western blotting at 1:500 dilution.

Date published: 2017-09-27

Rated 4 out of 5 by yuanye from A GOOD PRODUCTI used the antibody in western blot, and to test the mice liver samples. So, that's good!

Date published: 2017-09-19

Rated 5 out of 5 by 赵忠芳12 from very usefulIFN-gama antibody effect is good, immunohistochemical staining uniform, positive coloring significantly.

Date published: 2017-07-21

Rated 5 out of 5 by JM from Produced positive Western blot data of humanProduced positive Western blot data of human recombinant IFN- fusion protein. -SCBT QC

Date published: 2014-03-15

IFN-γ/Interferon gamma 抗体 (E-10) is rated 3.5 out of 5 by 6.

Santa Cruz Biotechnology, Inc. 是生物医学研究市场产品开发的世界领导者。请拨打0086-021-60936350联系我们。
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IFN-γ/Interferon gamma 抗体 (E-10): sc-373727

IFN-γ/Interferon gamma 抗体 (E-10) 规格

替代产品名称: IFN-γ

产品克隆名称: E-10

分类: 一抗

Clonality: 单克隆抗体

抗体同型: IgG_2a kappa light chain

氨基酸: 25-50

抗原表位靶标: IFN-γ

抗原表位种属: human

产品反应性/检测: precursor and mature IFN-γ

种属反应性/检测: human

其它哺乳动物反应性: N/A

其它非哺乳动物反应性: N/A

应用: WB, IP, IF, ELISA

瓶的信息: Each vial contains 200 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

研究引文: 15篇各种科学出版物的引文

優選的 IFN-γ/Interferon gamma 抗體是 IFN-γ/Interferon gamma 抗體 (G-23): sc-8423.