Date published: 2026-10-24

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ICAM-1/CD54 Antibody (6.5B5) - Western Blotting - Image 17219
ICAM-1 抗体 (6. 5B5): sc-18853. 在非还原条件下, 对 Raji (A), U266 (B) 和 SK-MEL-28 (C) 全细胞裂解液中 ICAM-1 的表达进行 Western 印迹分析.
ICAM-1 抗体 (6. 5B5): sc-18853. 在非还原条件下, 对 Raji (A), U266 (B) 和 SK-MEL-28 (C) 全细胞裂解液中 ICAM-1 的表达进行 Western 印迹分析.

ICAM-1/CD54 抗体 (6.5B5): sc-18853

4.8(9)
寫評論提問
说明书
  • ICAM-1/CD54抗体(6.5B5)是小鼠单克隆IgG1 κ, 在38篇文献中引用，规格为200 µg/ml
  • 针对TNF激活的human脐静脉内皮细胞
  • 抗-ICAM-1/CD54 抗体 (6.5B5) 推荐用于 WB, IP, IF 和 FCM，检测mouse, rat 和human 来源的 ICAM-1
  • 抗ICAM-1/CD54抗体（6.5B5）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • m-IgG Fc BP-HRP和 m-IgG1 BP-HRP是ICAM-1/CD54 Antibody (6.5B5) for WB applications. 的首选辅助检测试剂，这些试剂现与ICAM-1/CD54 Antibody (6.5B5) 打包提供（请参阅下面的订购信息）。
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描述
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関連項目

额外ICAM抗体，包括ICAM-1, ICAM-2和ICAM-3

ICAM-1/CD54抗体（6.5B5）是一种IgG1 κ小鼠单克隆ICAM-1抗体，可通过WB、IP、IF和FCM检测来自小鼠、大鼠和人类的ICAM-1。ICAM-1/CD54抗体（6.5B5）既可以是非偶联的抗ICAM-1抗体形式，也可以是多种偶联形式的抗ICAM-1抗体，包括琼脂、HRP、PE、FITC和多种Alexa Fluor®偶联物。细胞粘附分子（CAMs）是一类密切相关的细胞表面糖蛋白，参与生长期间的细胞间相互作用，被认为在胚胎发育和发展中发挥重要的、独立的作用。内细胞粘附分子-1（ICAM-1），也称为CD54，是免疫球蛋白超家族的一种基本膜蛋白，可识别β2α1和β2αM整合素。ICAM-2作为淋巴细胞功能相关抗原-1（LFA-1）的配体，参与白细胞粘附。ICAM-3在人类嗜酸性粒细胞表面高度表达，结合配体时可能抑制嗜酸性粒细胞炎症反应和存活。ICAM-4，也称为LW糖蛋白，与积合体αLβ2、αMβ2、α4β1、αV家族和αIIbβ3相互作用，与不同整合素的选择性结合可能与许多红细胞相关疾病的病理学有关。最后，ICAM-5表达于大脑前叶神经元表面，结合CD11a/CD18，因此可能作为中枢神经系统中白细胞结合的粘附分子。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

ICAM-1/CD54 抗体 (6.5B5) 参考文献:

  1. ICAM-1在肝癌发生和发展中的表达和作用。  |  Zhu, XW. and Gong, JP. 2013. Asian Pac J Cancer Prev. 14: 1579-83. PMID: 23679239
  2. 自噬促进细胞因子诱导的ICAM-1表达。  |  Wang, CY., et al. 2014. Innate Immun. 20: 200-13. PMID: 23751820
  3. 癌干细胞中的ICAM-1相关非编码RNA维持肝细胞癌中的ICAM-1表达。  |  Guo, W., et al. 2016. Clin Cancer Res. 22: 2041-50. PMID: 26667486
  4. ICAM-1在醛固酮诱导的动脉粥样硬化中的重要作用。  |  Marzolla, V., et al. 2017. Int J Cardiol. 232: 233-242. PMID: 28089144
  5. ICAM-1 E469K多态性在糖尿病肾病中的遗传和生物学效应。  |  Zhang, X., et al. 2020. J Diabetes Res. 2020: 8305460. PMID: 32626783
  6. 巨噬细胞 ICAM-1 在 LPS 诱导的内毒素血症中发挥着吞噬调节器的功能。  |  Zhong, H., et al. 2021. Inflamm Res. 70: 193-203. PMID: 33474594
  7. Hspa8和ICAM-1作为损伤诱导的γδT细胞活化的介质。  |  Johnson, MD., et al. 2022. J Leukoc Biol. 111: 135-145. PMID: 33847413
  8. Cordycerebroside A抑制ICAM-1依赖性M1单核细胞与骨关节炎滑膜成纤维细胞的粘附。  |  Lee, KT., et al. 2022. J Food Biochem. 46: e14108. PMID: 35165902
  9. NMT1维持ICAM-1以调节肿瘤细胞的粘附和迁移。  |  Wang, H., et al. 2023. Cell Signal. 109: 110739. PMID: 37269961
  10. 过敏患者上皮细胞上的ICAM-1：过敏炎症的特征。  |  Canonica, GW., et al. 1995. Int Arch Allergy Immunol. 107: 99-102. PMID: 7613226

订购信息

产品名称产品编号规格价格数量收藏夹

ICAM-1/CD54 抗体 (6.5B5)

sc-18853
200 µg/ml
CNY2422.00

ICAM-1/CD54 (6.5B5): m-IgG Fc BP-HRP 套装

sc-526581
200 µg Ab; 10 µg BP
CNY2715.00

ICAM-1/CD54 (6.5B5): m-IgG1 BP-HRP 套装

sc-531954
200 µg Ab; 20 µg BP
CNY2715.00

ICAM-1/CD54 抗体 (6.5B5) AC

sc-18853 AC
500 µg/ml, 25% agarose
CNY3189.00

ICAM-1/CD54 抗体 (6.5B5) HRP

sc-18853 HRP
200 µg/ml
CNY2422.00

ICAM-1/CD54 抗体 (6.5B5) FITC

sc-18853 FITC
200 µg/ml
CNY2527.00

ICAM-1/CD54 抗体 (6.5B5) PE

sc-18853 PE
200 µg/ml
CNY2625.00

ICAM-1/CD54 抗体 (6.5B5) Alexa Fluor® 488

sc-18853 AF488
200 µg/ml
CNY2738.00

ICAM-1/CD54 抗体 (6.5B5) Alexa Fluor® 546

sc-18853 AF546
200 µg/ml
CNY2738.00

ICAM-1/CD54 抗体 (6.5B5) Alexa Fluor® 594

sc-18853 AF594
200 µg/ml
CNY2738.00

ICAM-1/CD54 抗体 (6.5B5) Alexa Fluor® 647

sc-18853 AF647
200 µg/ml
CNY2738.00

ICAM-1/CD54 抗体 (6.5B5) Alexa Fluor® 680

sc-18853 AF680
200 µg/ml
CNY2738.00

ICAM-1/CD54 抗体 (6.5B5) Alexa Fluor® 790

sc-18853 AF790
200 µg/ml
CNY2738.00

What is the recommended secondary reagent to be used with this mouse monoclonal primary?

Asked by: jerojero
We recommend using one our exclusive Mouse IgG Binding Proteins as a secondary detection reagent. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Answered by: Technical Support 14
Date published: 2017-01-26
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Rated 4 out of 5 by Aida from It works for WB and FCM applicationsIt works quite well for both WB and FCM, however, to see de band in WB you have to use non-reducing conditions and thus, to see correctly actin I have to make two gels because actin does not look good under non-reducing conditions. But it works very well and the induction with TNF alpha in HUVEC cells is detected perfectly in both applications.
Date published: 2022-01-06
Rated 5 out of 5 by FZC123 from western BlotLast week, ICAM-1 antibody produced a strong bland in important experiment . My boss was satisfied with experiment result.
Date published: 2017-06-01
Rated 5 out of 5 by Germaine from Recommended for FCMStrong results in FCM in human peripheral blood leukocytes. Several good references for Flow in HUVECs in the literature too.
Date published: 2016-12-16
Rated 4 out of 5 by FL from Gives strong and unique band by westernGives strong and unique band by western-blot (non-reducing conditions!!), and nice staining by IF. Publication quality.
Date published: 2015-09-10
Rated 5 out of 5 by JL from Strong band in WB in human HPAECsStrong band in WB in human HPAECs, MLVECs. -SCBT Publication Review
Date published: 2015-04-12
Rated 5 out of 5 by HC from Robust signal in WB in RajiRobust signal in WB in Raji , U266 and SK-MEL-28 whole cell lysates. -SCBT QC
Date published: 2015-04-07
Rated 5 out of 5 by JM from Produced superb immunofluorescence membraneProduced superb immunofluorescence membrane staining in methanol-fixed HeLa cells. -SCBT QC
Date published: 2015-03-17
Rated 5 out of 5 by SH from Good publishable data in IHC in human endothelialGood publishable data in IHC in human endothelial cells. -SCBT Publication Review
Date published: 2015-01-11
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ICAM-1/CD54 抗体 (6.5B5) is rated 4.8 out of 5 by 9.
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