Date published: 2025-9-8
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ICAM-1/CD54 Antibody (15.2) - Western Blotting - Image 350714
ICAM-1 抗体 (15. 2): sc-107. 在 SK-N-MC (A), Jurkat (B), Raji (C), U266 (D) 和 TF-1 (E) 全细胞裂解液中 ICAM-1 表达的 Western 印迹分析.
ICAM-1 抗体 (15. 2): sc-107. 在 SK-N-MC (A), Jurkat (B), Raji (C), U266 (D) 和 TF-1 (E) 全细胞裂解液中 ICAM-1 表达的 Western 印迹分析.

ICAM-1/CD54 抗体 (15.2): sc-107

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说明书
  • ICAM-1/CD54抗体(15.2)是小鼠单克隆IgG1 κ, 在152篇文献中引用，规格为200 µg/ml
  • 针对ICAM-1阳性细胞制备
  • 抗-ICAM-1/CD54 抗体 (15.2) 推荐用于 WB, IP, IF, IHC(P) 和 FCM，检测mouse, rat 和human 来源的 ICAM-1
  • 抗ICAM-1/CD54抗体（15.2）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 还可偶联Alexa Fluor® 405 用于IF, IHC(P) 和FCM
  • 还提供无叠氮化物，用于生物学研究，sc-107 L，200 µg/0.1 ml
  • 关于如何获取ICAM-1/CD54 (15.2): sc-107的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP 和 m-IgG1 BP-HRP 是 ICAM-1/CD54 抗体 (15.2) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与ICAM-1/CD54 抗体 (15.2) 组合成套装提供。 (请参阅下面的订购信息)。

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描述
基因信息
说明书与实验方案
研究信息

関連項目

此外 ICAM 抗体 包括 ICAM-1, ICAM-2和ICAM-3

ICAM-1/CD54抗体（15.2）是一种IgG1 κ小鼠单克隆ICAM-1抗体，可通过WB、IP、IF、IHC（P）和FCM检测来自小鼠、大鼠和人类的ICAM-1。ICAM-1/CD54抗体（15.2）既可以是非偶联的抗ICAM-1抗体形式，也可以是多种偶联形式的抗ICAM-1抗体，包括琼脂、HRP、PE、FITC和多种Alexa Fluor®偶联物。细胞粘附分子（CAMs）是一类密切相关的细胞表面糖蛋白，参与生长期间的细胞间相互作用，被认为在胚胎发育和发展中发挥重要且独立的作用。内细胞粘附分子-1（ICAM-1），也称为CD54，是免疫球蛋白超家族的一种基本膜蛋白，可识别β2α1和β2αM积合体。ICAM-2作为淋巴细胞功能相关抗原-1（LFA-1）的配体，参与白细胞粘附。ICAM-3在人类嗜酸性粒细胞表面高度表达，结合配体时可能抑制嗜酸性粒细胞炎症反应和存活。ICAM-4，也称为LW糖蛋白，与积合体αLβ2、αMβ2、α4β1、αV家族和αIIbβ3相互作用，与不同积合体的选择性结合可能与许多红细胞相关疾病的病理学有关。最后，ICAM-5表达于大脑前叶神经元表面，结合CD11a/CD18，因此可能作为中枢神经系统中白细胞结合的粘附分子。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

订购信息

产品名称产品编号规格价格数量收藏夹

ICAM-1/CD54 抗体 (15.2)

sc-107
200 µg/ml
RMB2377.00

ICAM-1/CD54 (15.2): m-IgG Fc BP-HRP 套装

sc-526450
200 µg Ab; 10 µg BP
RMB2662.00

ICAM-1/CD54 (15.2): m-IgG1 BP-HRP 套装

sc-531823
200 µg Ab; 20 µg BP
RMB2662.00

ICAM-1/CD54 抗体 (15.2) L

sc-107 L
200 µg/0.1 ml
RMB2377.00

ICAM-1/CD54 抗体 (15.2) AC

sc-107 AC
500 µg/ml, 25% agarose
RMB3129.00

ICAM-1/CD54 抗体 (15.2) HRP

sc-107 HRP
200 µg/ml
RMB2377.00

ICAM-1/CD54 抗体 (15.2) FITC

sc-107 FITC
200 µg/ml
RMB2482.00

ICAM-1/CD54 抗体 (15.2) PE

sc-107 PE
200 µg/ml
RMB2580.00

ICAM-1/CD54 抗体 (15.2) Alexa Fluor® 488

sc-107 AF488
200 µg/ml
RMB2685.00

ICAM-1/CD54 抗体 (15.2) Alexa Fluor® 546

sc-107 AF546
200 µg/ml
RMB2685.00

ICAM-1/CD54 抗体 (15.2) Alexa Fluor® 594

sc-107 AF594
200 µg/ml
RMB2685.00

ICAM-1/CD54 抗体 (15.2) Alexa Fluor® 647

sc-107 AF647
200 µg/ml
RMB2685.00

ICAM-1/CD54 抗体 (15.2) Alexa Fluor® 680

sc-107 AF680
200 µg/ml
RMB2685.00

ICAM-1/CD54 抗体 (15.2) Alexa Fluor® 790

sc-107 AF790
200 µg/ml
RMB2685.00

ICAM-1/CD54 抗体 (15.2) Alexa Fluor® 405

sc-107 AF405
200 µg/ml
RMB2685.00

Is it known which exactly epitope, domain, or region of ICAM-1 is bound by this antibody? Thank you in advance.

Asked by: Josep IBEC
Thank you for your question. No epitope mapping has been performed for this antibody yet, so its binding site on the target protein is not known.
Answered by: Tech Support Europe
Date published: 2021-03-24

É necessário fazer uma permeabilização prévia para este anticorpo? Pretendo usá-lo para IHC em parafina. Obrigada.

Asked by: AnaC1
Thank you for your question. We recommend using sodium citrate buffer (pH 6.0) with heat treatment for the antigen retrieval method when using this antibody on formalin-fixed paraffin-embedded tissue sections, or for IHC(P). Details for this protocol are found in the link below. https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2019-04-25

How many Flowcytometry and IHC tests could be conducted with your supplied antibody reagents for ICAM1, VE cad and ZO1? Its urgent so please let me know asap!

Asked by: Paroma
Thank you for your question. The recommended starting dilution for IHC is 1:50 (range of 1:25-1:500). The number of slides that can be stained depend on your dilution, as well as your working volume.
Answered by: Technical Support
Date published: 2019-01-26

What is the expected band size for ICAM-1 using sc-107 for western blot?

Asked by: anuja
Thank you for your question. The expected molecular weight for ICAM-1 is 85-110 kDa due to glycosylation. There is representative Western blot data on our website for this specific antibody.
Answered by: Technical Support
Date published: 2018-12-21

What negative control should be used when using the conjugated version of this antibody in flow cytometry?

Asked by: AbPolly
Thank you for your inquiry. We recommend using the isotype specific normal mouse IgG conjugated to the same fluorophore. For example, if you are using sc-107 PE, then you would want to use normal mouse IgG1 PE, sc-2866.
Answered by: Technical Support 12
Date published: 2017-01-04
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Rated 4 out of 5 by from Good antibodyWorks well in WB (1/100). strong and clear binding in HUVEC cells.
Date published: 2018-04-24
Rated 4 out of 5 by from Anticorps validéTest de cet anticorps sur lignée humaine (kératinocyte et myoblaste) en cytomètrie en flux -> validé
Date published: 2018-03-21
Rated 4 out of 5 by from lovely productwe like it, my work need a lot of this, this one tried one, works good,
Date published: 2017-07-24
Rated 5 out of 5 by from Great antibody for multiple purposesICAM-1 Antibody (15.2) is a very well cited antibody. Strong and specific results produced by IHC (P), WB, IF and FCM. Highly recommended!
Date published: 2017-01-11
Rated 4 out of 5 by from Very nice productGreat for if staining, wb and wb applications but not quite as good as the icam-1(g-5) clone; both are better than the polyclonal alternatives
Date published: 2016-12-31
Rated 5 out of 5 by from Good publishable data in ELISA and IHC inGood publishable data in ELISA and IHC in human HUVEC cells. -SCBT Publication Review
Date published: 2015-05-12
Rated 5 out of 5 by from Strong band in WB in SW480 and HeLa wholeStrong band in WB in SW480 and HeLa whole cell lysates. -SCBT QC
Date published: 2015-03-03
Rated 5 out of 5 by from Superb FCM data of human peripheral bloodSuperb FCM data of human peripheral blood leukocytes. -SCBT QC
Date published: 2015-02-18
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ICAM-1/CD54 抗体 (15.2) is rated 4.6 out of 5 by 10.
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