Date published: 2025-9-6
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HXK II Antibody (B-8) - Western Blotting - Image 377583
HXK II 抗体 (B-8): sc-374091. Hep G2 (A) 和 SJRH30 (B) 全细胞裂解液中 HXK II 表达的 Western 印迹分析.
HXK II 抗体 (B-8): sc-374091. Hep G2 (A) 和 SJRH30 (B) 全细胞裂解液中 HXK II 表达的 Western 印迹分析.

HXK II 抗体 (B-8): sc-374091

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说明书
  • HXK II 抗体 B-8 是小鼠单克隆 IgG2a κ，HXK II抗体, 在42篇文献中引用，规格为200 µg/ml
  • 针对human起源的HXK II内部区域内的氨基酸316-410进行
  • HXK II 抗体 (B-8) 推荐用于WB, IP, IF, IHC(P) 和 ELISA，检测human 来源的 HXK II
  • 抗HXK II抗体（B-8）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取HXK II (B-8): sc-374091的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP, m-IgG2a BP-HRP 和 m-IgGκ BP-HRP 是 HXK II 抗体 (B-8) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与HXK II 抗体 (B-8) 组合成套装提供。 (请参阅下面的订购信息)。

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相关产品
描述
基因信息
蛋白序列
说明书与实验方案
研究信息

関連項目

此外 HXK 抗体 包括 HXK I, HXK II和HXK III

HXK II抗体（B-8）是一种IgG2a κ小鼠单克隆HXK II抗体（也称为己糖激酶II抗体、己糖激酶-B抗体或肌肉型己糖激酶抗体），可通过WB、IP、IF、IHC(P)和ELISA检测人源的HXK II蛋白。HXK II抗体（B-8）既可以是非偶联的抗HXK II抗体形式，也可以是多种偶联形式的抗HXK II抗体，包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®偶联物。己糖激酶利用Mg-ATP作为磷酸供体，催化细胞内葡萄糖代谢的第一步，即葡萄糖转化为葡萄糖-6-磷酸。已经鉴定出四种己糖激酶同工酶，包括己糖激酶I（HXK I）、己糖激酶II（HXK II）、己糖激酶III（HXK III）和己糖激酶IV（HXK IV，也称为葡萄糖激酶或GCK）。己糖激酶I-III各自包含一个N端疏水性氨基酸簇。葡萄糖激酶缺乏N端疏水性簇。该疏水性簇被认为对于膜结合是必要的。这得到了以下发现的证实：葡萄糖激酶对葡萄糖的亲和力低于其他己糖激酶。HXK I已被证明在脑、肾和心脏组织以及肝癌细胞系中表达。HXK II参与脂肪和骨骼组织对葡萄糖的摄取和利用。在己糖激酶中，HXK III对葡萄糖的亲和力最高。葡萄糖激酶在胰腺β细胞中表达，作为葡萄糖传感器发挥作用，决定胰岛素分泌的"设定点"。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

HXK II 抗体 (B-8) 参考文献:

  1. 人类葡萄糖激酶基因：与早发非胰岛素依赖型（2 型）糖尿病有关的两个错义突变的分离, 特征和鉴定。  |  Stoffel, M., et al. 1992. Proc Natl Acad Sci U S A. 89: 7698-702. PMID: 1502186
  2. 肿瘤细胞中的葡萄糖磷酸化。对编码线粒体结合型己糖激酶的全长 cDNA 进行克隆, 测序和活性过表达。  |  Arora, KK., et al. 1990. J Biol Chem. 265: 6481-8. PMID: 2318862
  3. Hexokinase 2 as a novel selective metabolic target for rheumatoid arthritis.  |  Bustamante, MF., et al. 2018. Ann Rheum Dis. 77: 1636-1643. PMID: 30061164
  4. 微生物对己糖激酶 2 的调控将结肠炎中的线粒体代谢与细胞死亡联系在一起。  |  Hinrichsen, F., et al. 2021. Cell Metab. 33: 2355-2366.e8. PMID: 34847376
  5. 在帕金森病中，己糖激酶 2 的表达上调会促进乳酸的产生，从而诱导多巴胺能神经元凋亡。  |  Li, J., et al. 2022. Neurobiol Dis. 163: 105605. PMID: 34973450
  6. CRISPR/Cas9筛选揭示了己糖激酶2通过激活ACSL4-脂肪酸β氧化途径增强癌症干细胞和肿瘤发生。  |  Li, H., et al. 2022. Adv Sci (Weinh). 9: e2105126. PMID: 35603967
  7. 在急性髓细胞性白血病和正常造血干细胞和祖细胞中，代谢酶己糖激酶2定位在细胞核内，以保持干性。  |  Thomas, GE., et al. 2022. Nat Cell Biol. 24: 872-884. PMID: 35668135
  8. 己糖激酶 2 在小细胞肺癌中的非代谢功能：通过增加 USP11 介导的 CD133 稳定性来促进癌细胞干性。  |  Wang, J., et al. 2022. Cancer Commun (Lond). 42: 1008-1027. PMID: 35975322
  9. 己糖激酶2在炎症相关疾病及其抑制剂方面的研究进展。  |  Chen, J., et al. 2024. Eur J Med Chem. 264: 115986. PMID: 38011767
  10. 大鼠体内多种形式的己糖激酶：组织分布, 年龄依赖性和特性。  |  Katzen, HM. and Schimke, RT. 1965. Proc Natl Acad Sci U S A. 54: 1218-25. PMID: 5219826
  11. 人类己糖激酶 II：序列和与其他己糖激酶的同源性。  |  Deeb, SS., et al. 1993. Biochem Biophys Res Commun. 197: 68-74. PMID: 8250948
  12. 以融合蛋白形式表达的人III型己糖激酶羧基域的纯化和特征。  |  Palma, F., et al. 1996. Mol Cell Biochem. 155: 23-9. PMID: 8717435

订购信息

产品名称产品编号规格价格数量收藏夹

HXK II 抗体 (B-8)

sc-374091
200 µg/ml
RMB2377.00

HXK II (B-8): m-IgG Fc BP-HRP 套装

sc-529656
200 µg Ab; 10 µg BP
RMB2662.00

HXK II (B-8): m-IgGκ BP-HRP 套装

sc-522616
200 µg Ab, 40 µg BP
RMB2662.00

HXK II (B-8): m-IgG2a BP-HRP 套装

sc-547351
200 µg Ab; 10 µg BP
RMB2662.00

HXK II 抗体 (B-8) AC

sc-374091 AC
500 µg/ml, 25% agarose
RMB3129.00

HXK II 抗体 (B-8) HRP

sc-374091 HRP
200 µg/ml
RMB2377.00

HXK II 抗体 (B-8) FITC

sc-374091 FITC
200 µg/ml
RMB2482.00

HXK II 抗体 (B-8) PE

sc-374091 PE
200 µg/ml
RMB2580.00

HXK II 抗体 (B-8) Alexa Fluor® 488

sc-374091 AF488
200 µg/ml
RMB2685.00

HXK II 抗体 (B-8) Alexa Fluor® 546

sc-374091 AF546
200 µg/ml
RMB2685.00

HXK II 抗体 (B-8) Alexa Fluor® 594

sc-374091 AF594
200 µg/ml
RMB2685.00

HXK II 抗体 (B-8) Alexa Fluor® 647

sc-374091 AF647
200 µg/ml
RMB2685.00

HXK II 抗体 (B-8) Alexa Fluor® 680

sc-374091 AF680
200 µg/ml
RMB2685.00

HXK II 抗体 (B-8) Alexa Fluor® 790

sc-374091 AF790
200 µg/ml
RMB2685.00

Which specific antigen retrieval do you recommand for HXK II Antibody (B-8): sc-374091 for IHC(P)? Your protocol (immunoperoxidase-staining Antigen retrieval is described in step C) does not specify for this antibody specifically.

Asked by: Nina 44
Thank you for your question. For antigen retrieval in experiments with this antibody we recommend heat treatment: Heat treatment (recommended method): Place slides in a container and cover with 10 mM sodium citrate buffer, pH 6.0 (sc-294091); or with 50 mM glycine-HCl buffer (sc-29096), pH 3.5, with 0.01% (w/v) EDTA (sc-29092). Heat at 95° C for 5 minutes. Top off with fresh buffer and heat at 95° C for 5 minutes (optimal incubation time may vary for each tissue type). Allow slides to cool in the buffer for approximately 20 minutes. Wash in deionized H2O three times for 2 minutes each. Aspirate excess liquid from slides.
Answered by: Tech Support Europe
Date published: 2022-06-21

Can HXK II Antibody (B-8): sc-374091 be used to stain formalin-fixed, paraffin-embedded (FFPE) tissue sections? Which antigen retrieval do you recommand?

Asked by: Nina 44
Thank you for your question. sc-374091 is recommended to be used in IHC(P). You can find a detailed protocol by following the link below: https://www.scbt.com/resources/protocols/immunoperoxidase-staining Antigen retrieval is described in step C.
Answered by: Tech Support Europe
Date published: 2022-06-21

Does this antibody only recognise human HK2 or is there other species reactivity?

Asked by: ABquestion
Thank you for your question. This mouse monoclonal antibody has only been validated for human use at this time, and can not be recommended or warrantied for use on mouse samples. If you have any further questions, please contact Technical Service.
Answered by: Technical Support
Date published: 2018-12-11

What is the recommended starting dilution for Western blot for HXK II (B-8): sc-374091?

Asked by: jenni
Thank you for your question. The recommended starting dilution for Western blot is 1:100 with a dilution range of 1:100-1:1000. This information, along with other technical specifications, can be found in the “datasheet” link at the top of the product page, for which I have provided the link: https://datasheets.scbt.com/sc-374091.pdf If you have any further questions or concerns, please contact our Technical Service department by calling 800-457-3801 option 2, emailing [email protected], or using the Live Chat function on our website.
Answered by: Technical Support 13
Date published: 2017-01-26
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Rated 5 out of 5 by from 非常好用靶蛋白条带单一且清晰，与理论分子量完全吻合，无杂带干扰，特异性堪称完美 -即便在样本蛋白浓度极低时，依然能精准锁定目标条带，无交叉反应
Date published: 2025-09-04
Rated 4 out of 5 by from This antibody worked well in WBThis antibody worked well in WB using human human macrophages
Date published: 2025-06-06
Rated 5 out of 5 by from Easy to use, works as intendedWorks as intended, used it in HEK293T lysates in WB (1:1000).
Date published: 2023-12-21
Rated 4 out of 5 by from Strong clear WB signalUsed this antibody in WB applications and to validate KO cell lines. Signal was bright and clear and antibody was used more diluted than recommended
Date published: 2021-02-25
Rated 5 out of 5 by from Works wellWorks well with human cancer cell lines. Was used to confirm siRNA efficacy
Date published: 2018-12-20
Rated 5 out of 5 by from WBI got clear bands and correct position by wextern blot (1：200).
Date published: 2018-03-01
Rated 3 out of 5 by from Works but was hoping for betterUsed on human stem cells. 20 ug loaded, 1:100 dilution. I got clear bands the first time. but the second time I reused/recycled the antibody, I got background and not so clear bands. So perhaps better to you a freshly prepared antibody every time.
Date published: 2018-02-14
Rated 4 out of 5 by from Good result for WBWestern Blot analysis of Hexokinase 2 expression in human endothelial whole cell lysates (both lanes)
Date published: 2018-02-12
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HXK II 抗体 (B-8) is rated 4.4 out of 5 by 17.
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