Date published: 2025-9-8

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HURP 抗体 (D-12): sc-376760

5.0(2)
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说明书
  • HURP抗体(D-12)是小鼠单克隆IgG3 (kappa轻链),规格为200 µg/ml
  • 特异性抗原位于human物种的HURP的内部区域内的氨基酸417-451之间
  • 推荐用于 human 来源的HURP WB, IP, IF, IHC(P) 和 ELISA检测
  • m-IgG3 BP-HRP 是HURP 抗体 (D-12) 在 WB and IHC(P) 应用中的首选二抗检测试剂。该试剂现与HURP 抗体 (D-12) 组合成套装提供。 (请参阅下面的订购信息)。

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    関連項目

    HURP(肝癌上调蛋白),也被称为DLGAP5(与Disks large相关蛋白5)、DLG7或DLG1,是一个由846个氨基酸组成的蛋白质,它定位于细胞核和细胞质,特别是在有丝分裂细胞的纺锤极中。HURP在睾丸、结肠、骨髓、胎盘和胎儿肝脏中表达,被认为是一种细胞周期调节因子,与Cdc2 p34相互作用,并在上皮细胞中介导粘附连接的组装和分化。HURP在细胞周期的G2/M期上调,并可能通过细胞周期控制在致癌作用和肿瘤转化中发挥作用。在DNA损伤时,HURP被ATM或ATR磷酸化。此外,HURP还受到泛素诱导的蛋白酶体降解的调控。由于选择性剪接事件的存在,HURP存在两种异构体。

    仅限研究使用。不适用于诊断和治疗用途。

    Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

    LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

    HURP 抗体 (D-12) 参考文献:

    1. HURP 在非小细胞肺癌中的预后和预测价值。  |  Wang, Q., et al. 2018. Oncol Rep. 39: 1682-1692. PMID: 29484418
    2. 在细胞迁移过程中,PRMT5/HURP 轴通过稳定乙酰基微管蛋白和高尔基体来延缓高尔基体的重新定位。  |  Chiu, SC., et al. 2022. J Cell Physiol. 237: 1033-1043. PMID: 34541678
    3. 有证据表明,在动子核心-微管中存在 HURP/EB 自由混合核苷酸区。  |  Castrogiovanni, C., et al. 2022. Nat Commun. 13: 4704. PMID: 35948594
    4. JMJD6/HURP轴通过NF-κB依赖的中心体重新定位和Cdc42介导的高尔基体重新定位促进细胞迁移。  |  Huang, YJ., et al. 2022. J Cell Physiol. 237: 4517-4530. PMID: 36250981
    5. 新月形高尔基体带由 Ajuba/PRMT5/Aurora-A 复合物修饰的 HURP 形成。  |  Chiu, SC., et al. 2023. Cell Commun Signal. 21: 156. PMID: 37370099
    6. HURP 在分裂期的定位是一个多步骤过程的结果,需要在 Ser627 残基上进行磷酸化。  |  Didaskalou, S., et al. 2023. Front Cell Dev Biol. 11: 981425. PMID: 37484914
    7. HURP 和 Kif18A 在有丝分裂纺锤体调控中的分子相互作用  |  Perez-Bertoldi, JM., et al. 2024. bioRxiv.. PMID: 38645125
    8. HURP与β-微管蛋白的长春花结构域结合导致癌症抗药性。  |  Saju, A., et al. 2024. Nat Commun. 15: 8844. PMID: 39397030
    9. HURP 可调节 Kif18A 的招募和活性,从而协同控制微管动力学。  |  Perez-Bertoldi, JM., et al. 2024. Nat Commun. 15: 9687. PMID: 39516196
    10. HURP 通过稳定微管并与 TPX2 协同作用,促进纺锤体的组装。  |  Valdez, VA., et al. 2024. Nat Commun. 15: 9689. PMID: 39516491

    订购信息

    产品名称产品编号规格价格数量收藏夹

    HURP 抗体 (D-12)

    sc-376760
    200 µg/ml
    RMB2377.00

    HURP (D-12): m-IgG3 BP-HRP 套装

    sc-550462
    200 µg Ab; 40 µg BP
    RMB2662.00

    HURP (D-12) 中和勝肽

    sc-376760 P
    100 µg/0.5 ml
    RMB511.00

    What is the difference between this Ab and Ab HURP (E-7):sc-377004 ?(except the fact that is a monoclonal IgG3 versus IgG1).One is more recommended for a specific application?Do both work for IF after glutaraldhyde fixation? Thanks

    Asked by: cedricc
    Thank you for your question. The antibodies differ in their Ig isotypes and application recommendations. They have been raised against the same epitope region but are specific against different target sites within those. HURP Antibody (D-12): sc-376760 is only recommended for human cells and tissues, but apart from that, either of the two antibodies would be suitable for IF applications.
    Answered by: Tech Support Europe
    Date published: 2020-11-02

    What application is the blocking peptide sc-376760 P appropriate for?

    Asked by: cjMara
    Thank you for your question. The blocking peptide is intended for use as a negative control, by pre-adsorbing the mouse monoclonal antibody against the antigen. For full protocol details, please contact our Technical Services Department or view our online protocol here: https://www.scbt.com/scbt/resources/protocols/peptide-neutralization
    Answered by: Technical Support
    Date published: 2017-02-27
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    Rated 5 out of 5 by from Great immunoperoxidase nuclear and cytoplasmicGreat immunoperoxidase nuclear and cytoplasmic staining in formalin fixed, paraffin-embedded human testis tissue. -SCBT QC
    Date published: 2015-05-14
    Rated 5 out of 5 by from Good Western Blot data of HURP expression in KGood Western Blot data of HURP expression in K-562 nuclear extract. -SCBT QC
    Date published: 2014-09-12
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    HURP 抗体 (D-12) is rated 5.0 out of 5 by 2.
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