Date published: 2025-9-6
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HuD Antibody (H-9) - Western Blotting - Image 12793
HuD 抗体 (H-9): sc-48421. 在 SK-N-SH (A) 和 SH-SY5Y (B) 全细胞裂解液以及小鼠脑 (C) 和大鼠脑 (D) 组织提取物中对 HuD 表达的 Western 印迹分析.
HuD 抗体 (H-9): sc-48421. 在 SK-N-SH (A) 和 SH-SY5Y (B) 全细胞裂解液以及小鼠脑 (C) 和大鼠脑 (D) 组织提取物中对 HuD 表达的 Western 印迹分析.

HuD 抗体 (H-9): sc-48421

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说明书
  • HuD 抗体 H-9 是小鼠单克隆 IgG2a κ，HuD抗体, 在21篇文献中引用，规格为200 µg/ml
  • 免疫human物种的HuD的氨基酸1-300
  • HuD 抗体 (H-9) 推荐用于 WB, IP, IF, IHC(P) 和 ELISA，检测mouse, rat 和human 来源的 HuD
  • 抗HuD抗体（H-9）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取HuD (H-9): sc-48421的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP, m-IgG2a BP-HRP 和 m-IgGκ BP-HRP 是 HuD 抗体 (H-9) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与HuD 抗体 (H-9) 组合成套装提供。 (请参阅下面的订购信息)。

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相关产品
描述
基因信息
蛋白序列
说明书与实验方案
研究信息

関連項目

此外 Hu 抗体 包括 HuC, HuD和HuR

HuD 抗体（H-9）是一种小鼠单克隆 IgG2a kappa 轻链抗体，可通过西部印迹（WB）、免疫沉淀（IP）、免疫荧光（IF）、石蜡包埋切片免疫组织化学（IHCP）和酶联免疫吸附试验（ELISA）检测小鼠、大鼠和人源的 HuD 蛋白。抗 HuD 抗体 (H-9) 有非共轭型和多种共轭型，包括琼脂糖、辣根过氧化物酶 (HRP)、藻红蛋白 (PE)、异硫氰酸荧光素 (FITC) 和多种 Alexa Fluor® 共轭物。HuD 蛋白是 RNA 结合蛋白 Elav-like 家族的成员，在有丝分裂后的神经元中表达，在神经元分化和维持过程中起着至关重要的作用。HuD 含有三个 RNA 识别基序（RRM），可与 mRNA 转录本 3'- 非翻译区中的富含 AU 的元件（ARE）特异性结合。这种相互作用可调节 mRNA 的稳定性和降解，从而影响与细胞生长和分化相关的基因表达水平。通过与 ARE 位点结合，HuD 可抑制目标 mRNA 的快速降解，从而提高转录本的稳定性，这对神经元的正常功能和发育至关重要。HuD 的动态调控，包括在细胞核和细胞质之间的穿梭，确保了 mRNA 靶点的可及性，使 HuD 单克隆抗体 (H-9) 成为研究神经元生物学和相关疾病分子机制的宝贵工具。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

HuD 抗体 (H-9) 参考文献:

  1. HuD 和其他 RNA 结合蛋白在神经发育和可塑性中的作用  |  Perrone-Bizzozero, N. and Bolognani, F. 2002. J Neurosci Res. 68: 121-6. PMID: 11948657
  2. RNA 结合蛋白 HuD：神经元分化、维持和可塑性的调控因子。  |  Deschênes-Furry, J., et al. 2006. Bioessays. 28: 822-33. PMID: 16927307
  3. HuD 剪接变体在神经元分化中的替代作用  |  Hayashi, S., et al. 2015. J Neurosci Res. 93: 399-409. PMID: 25332105
  4. HuD 与神经元发育和突触可塑性相关基因的环状 RNA 结合并对其进行调控  |  Dell'Orco, M., et al. 2020. Front Genet. 11: 790. PMID: 32849796
  5. HuD 在分化的神经母细胞瘤细胞和散发性 ALS 运动皮层氧化应激过程中调节 SOD1 的表达。  |  Dell'Orco, M., et al. 2021. Neurobiol Dis. 148: 105211. PMID: 33271327
  6. RNA 结合蛋白 HuD 控制胰岛素翻译  |  Lee, EK., et al. 2020. Mol Cell. 80: 1135. PMID: 33338402
  7. 与 ALS 相关的 FUS 突变会改变运动神经元的轴突生长，并影响 HuD/ELAVL4 和 FMRP 的活性。  |  Garone, MG., et al. 2021. Commun Biol. 4: 1025. PMID: 34471224
  8. RNA 结合蛋白 HuD 通过抑制 mTORC1 活性和提高 ARL6IP1 水平促进自噬和肿瘤应激存活。  |  Bishayee, K., et al. 2022. J Exp Clin Cancer Res. 41: 18. PMID: 35012594
  9. 解密HuD介导的神经元分化中Akt1-HuD的相互作用  |  Nishisaka, H., et al. 2024. Biochimie. 221: 20-26. PMID: 38244852
  10. 在肌萎缩性脊髓侧索硬化症的体外和体内模型中，HuD（ELAVL4）功能增益会损害神经肌肉接头并诱导细胞凋亡。  |  Silvestri, B., et al. 2024. bioRxiv.. PMID: 38464028

订购信息

产品名称产品编号规格价格数量收藏夹

HuD 抗体 (H-9)

sc-48421
200 µg/ml
RMB2377.00

HuD (H-9): m-IgG Fc BP-HRP 套装

sc-528541
200 µg Ab; 10 µg BP
RMB2662.00

HuD (H-9): m-IgGκ BP-HRP 套装

sc-520939
200 µg Ab, 40 µg BP
RMB2662.00

HuD (H-9): m-IgG2a BP-HRP 套装

sc-547121
200 µg Ab; 10 µg BP
RMB2662.00

HuD 抗体 (H-9) AC

sc-48421 AC
500 µg/ml, 25% agarose
RMB3129.00

HuD 抗体 (H-9) HRP

sc-48421 HRP
200 µg/ml
RMB2377.00

HuD 抗体 (H-9) FITC

sc-48421 FITC
200 µg/ml
RMB2482.00

HuD 抗体 (H-9) PE

sc-48421 PE
200 µg/ml
RMB2580.00

HuD 抗体 (H-9) Alexa Fluor® 488

sc-48421 AF488
200 µg/ml
RMB2685.00

HuD 抗体 (H-9) Alexa Fluor® 546

sc-48421 AF546
200 µg/ml
RMB2685.00

HuD 抗体 (H-9) Alexa Fluor® 594

sc-48421 AF594
200 µg/ml
RMB2685.00

HuD 抗体 (H-9) Alexa Fluor® 647

sc-48421 AF647
200 µg/ml
RMB2685.00

HuD 抗体 (H-9) Alexa Fluor® 680

sc-48421 AF680
200 µg/ml
RMB2685.00

HuD 抗体 (H-9) Alexa Fluor® 790

sc-48421 AF790
200 µg/ml
RMB2685.00

Can you provide the binding epitope for this monoclonal antibody?

Asked by: yuki
Thank you for your question. This antibody is raised against amino acids 1-300 of HuD of human origin, the sepcific binding epitope is not characterized.
Answered by: Technical Service SH
Date published: 2023-09-25

HuD Antibody (H-9): sc-48421: Can you please provide the clone ID information?

Asked by: Leena Kumar
Thank you very much for your question. We produced this antibody against a recomb. protein fragment consisting of amino acids 1-300 of HuD of human origin. The same antigen was used for the other currently available antibody. https://www.scbt.com/scbt/browse/hud-Antibodies/_/N-11n1jcq The exact binding epitope for this monoclonal antibody has not been mapped, yet.
Answered by: Technical Support Europe
Date published: 2018-01-16

What is the total volume provided of HuD (H-9):sc-48421 monoclonal antibody?

Asked by: jenni
Thank you for your question. This antibody is provided as 200 µg in 1 ml total volume of PBS with < 0.1% sodium azide and 0.1% gelatin.
Answered by: Technical Support 4
Date published: 2017-01-13

Can HuD (H-9): sc-48421 monoclonal antibody be used to stain formalin-fixed, paraffin-embedded (FFPE) tissue sections?

Asked by: jenni
Thank you for your question. Yes, HuD (H-9): sc-48421 has been validated for use in IHC with paraffin-embedded sections. We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support 4
Date published: 2017-01-13
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Rated 5 out of 5 by from Strong signalHas good signal for paraffin-embedded sample for fluorescent immunohistochemistry.
Date published: 2017-05-16
Rated 5 out of 5 by from Superb for multiple applications !!Great IHC staining results; also strong WB; much better than polyclonal alternatives
Date published: 2017-01-19
Rated 5 out of 5 by from Great antibody for stainingThis HuD antibody shows good results for IHC in human tissue.
Date published: 2017-01-12
Rated 5 out of 5 by from great antibodyI tried this as a sample antibody from Santa Cruz and it really worked well on mouse intestine cryo sections at 1/ 400 dilution for myenteric ganglia.
Date published: 2016-12-23
Rated 5 out of 5 by from Working well in IF in zebrafishWorking well in IF in zebrafish.
Date published: 2016-08-08
Rated 5 out of 5 by from Were able to publish for use in IF in rat tissueWere able to publish for use in IF in rat tissue.
Date published: 2015-06-04
Rated 5 out of 5 by from Positive signal in WB in SKPositive signal in WB in SK-N-SH and SH-SY5Y whole cell lysates and mouse and rat brain tissue extracts. -SCBT QC
Date published: 2015-04-16
Rated 5 out of 5 by from Produced excellent immunoperoxidase nuclearProduced excellent immunoperoxidase nuclear and cytoplasmic staining in formalin fixed, paraffin-embedded human cerebral cortex tissue. -SCBT QC
Date published: 2015-03-15
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HuD 抗体 (H-9) is rated 5.0 out of 5 by 9.
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