Date published: 2025-9-8

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HuD 抗体 (E-1): sc-28299

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说明书
  • HuD抗体(E-1)是小鼠单克隆IgG2a κ, 在30篇文献中引用,规格为200 µg/ml
  • 免疫human物种的HuD的氨基酸1-300
  • 抗-HuD 抗体 (E-1) 推荐用于 WB, IP, IF, IHC(P) 和 ELISA,检测mouse, rat 和human 来源的 HuD
  • 抗HuD抗体(E-1)可与琼脂糖结合用于IP;与HRP结合用于WB、IHC(P)和ELISA;与藻红蛋白FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取HuD (E-1): sc-28299免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRPm-IgG2a BP-HRP 是 HuD 抗体 (E-1) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与HuD 抗体 (E-1) 组合成套装提供。 (请参阅下面的订购信息)。

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関連項目

HuD抗体(E-1)是一种小鼠单克隆IgG2a kappa轻链抗体,可通过蛋白质印迹(WB)、免疫沉淀(IP)、免疫荧光(IF)、免疫组织化学和酶联免疫吸附测定(ELISA )。抗HuD抗体(E-1)有非结合型和多种结合型,包括琼脂糖、辣根过氧化物酶(HRP)、藻红蛋白(PE)、异硫氰酸荧光素(FITC)和多种Alexa Fluor®结合物。HuD,也称为ELAVL4,在调节mRNA稳定性和翻译方面发挥着至关重要的作用,尤其是在神经元分化与维持至关重要的后神经元中。HuD与Elav样蛋白家族的其他成员一起,包含三个RNA识别基序,可特异性结合靶mRNA 3'-非翻译区中的AU富集元素。这种相互作用可以防止这些mRNA快速降解,从而提高转录本的稳定性,这对神经元的功能和发育至关重要。HuD能够在细胞核和细胞质之间穿梭,由核出口信号调节,这进一步强调了HuD在响应细胞信号控制基因表达中的重要性。HuD单克隆抗体(E-1)使研究人员能够有效地研究mRNA调控动态和HuD在神经发生和肿瘤发生等各种生物过程中的作用。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

HuD 抗体 (E-1) 参考文献:

  1. 哺乳动物ELAV样蛋白HuD诱导神经元分化需要细胞质定位。  |  Kasashima, K., et al. 1999. Genes Cells. 4: 667-83. PMID: 10620013
  2. HuD 在神经系统功能和病理学中的作用  |  Perrone-Bizzozero, N. and Bird, CW. 2013. Front Biosci (Schol Ed). 5: 554-63. PMID: 23277068
  3. HuD/ELAVl4基因新的复杂性;对神经元发育、功能和功能障碍的影响。  |  Bronicki, LM. and Jasmin, BJ. 2013. RNA. 19: 1019-37. PMID: 23861535
  4. RNA 结合蛋白 HuD 是神经元和非神经元系统中的多功能因子  |  Jung, M. and Lee, EK. 2021. Biology (Basel). 10: PMID: 33922479
  5. RNA 结合蛋白 HuD (ELAVL4) 在神经系统疾病中的新作用  |  Silvestri, B., et al. 2022. Int J Mol Sci. 23: PMID: 36498933
  6. ELAV 类 RNA 结合蛋白在人类神经母细胞瘤中的不同活性。  |  Chagnovich, D., et al. 1996. J Biol Chem. 271: 33587-91. PMID: 8969226
  7. Elav 样蛋白与 GAP-43 mRNA 3'- 非翻译区的保守调控元件结合。  |  Chung, S., et al. 1997. J Biol Chem. 272: 6593-8. PMID: 9045688
  8. 鉴定 HuR 蛋白与 AUUUA 介导的 mRNA 衰变有关。  |  Myer, VE., et al. 1997. EMBO J. 16: 2130-9. PMID: 9155038
  9. 神经内分泌基因 Hel-N1 和 HuD 在小细胞肺癌中的差异表达:HuD 在变异表型中下调的证据。  |  King, PH. 1997. Int J Cancer. 74: 378-82. PMID: 9291425
  10. 神经发生过程中 Hu RNA 结合蛋白的顺序表达和作用  |  Wakamatsu, Y. and Weston, JA. 1997. Development. 124: 3449-60. PMID: 9310339
  11. 富含 AU 元素的结合蛋白 HuR(一种 ELAV 蛋白)稳定 RNA。  |  Peng, SS., et al. 1998. EMBO J. 17: 3461-70. PMID: 9628881
  12. 神经元特异性 hel-N1 和 HuD 作为神经母细胞瘤的新型分子标记物:HuD 信使 RNA 水平与良好预后特征的相关性。  |  Ball, NS. and King, PH. 1997. Clin Cancer Res. 3: 1859-65. PMID: 9815574

订购信息

产品名称产品编号规格价格数量收藏夹

HuD 抗体 (E-1)

sc-28299
200 µg/ml
RMB2377.00

HuD (E-1): m-IgG Fc BP-HRP 套装

sc-525519
200 µg Ab; 10 µg BP
RMB2662.00

HuD (E-1): m-IgG2a BP-HRP 套装

sc-546302
200 µg Ab; 10 µg BP
RMB2662.00

HuD 抗体 (E-1) AC

sc-28299 AC
500 µg/ml, 25% agarose
RMB3129.00

HuD 抗体 (E-1) HRP

sc-28299 HRP
200 µg/ml
RMB2377.00

HuD 抗体 (E-1) FITC

sc-28299 FITC
200 µg/ml
RMB2482.00

HuD 抗体 (E-1) PE

sc-28299 PE
200 µg/ml
RMB2580.00

HuD 抗体 (E-1) Alexa Fluor® 488

sc-28299 AF488
200 µg/ml
RMB2685.00

HuD 抗体 (E-1) Alexa Fluor® 546

sc-28299 AF546
200 µg/ml
RMB2685.00

HuD 抗体 (E-1) Alexa Fluor® 594

sc-28299 AF594
200 µg/ml
RMB2685.00

HuD 抗体 (E-1) Alexa Fluor® 647

sc-28299 AF647
200 µg/ml
RMB2685.00

HuD 抗体 (E-1) Alexa Fluor® 680

sc-28299 AF680
200 µg/ml
RMB2685.00

HuD 抗体 (E-1) Alexa Fluor® 790

sc-28299 AF790
200 µg/ml
RMB2685.00

Is HuD (E-1): sc-28299 monoclonal antibody available directly conjugated to HRP or fluorescent dyes?

Asked by: jenniferc
Thank you for your question. All requests for directly conjugated versions of this antibody should be directed to our Special Orders team at [email protected] Alternatively, consider using HuD (H-9): sc-48421, which is available conjugated to agarose, HRP, FITC, PE, and more. (https://www.scbt.com/scbt/product/hud-antibody-h-9)
Answered by: Technical Support 7
Date published: 2017-01-22
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Rated 4 out of 5 by from goodWorks very well for Western Blotting at the dilution of 1:1000 overnight or 1 h at room temperature. Works very well also for Immunoprecipitation (4 g for 500 g total protein lysate).
Date published: 2021-04-22
Rated 1 out of 5 by from Antibody not specificBe careful with this antibody. It has been produced using almost the full ELAVL4 protein without taking into account the high sequence homology between ELAVL4 and ELAVL2 (91%), and with ELAVL3 (85%). I have observed the same specificy for ELAVL4, ELAVL2 and ELAVL4 using this antibody I'm really disappointed!!
Date published: 2018-10-24
Rated 5 out of 5 by from Excellent HuD antibody for WBThe E-1 clone works well in WB for mouse, rat and human.
Date published: 2016-12-22
Rated 5 out of 5 by from Produced great immunoperoxidase nuclear stainingProduced great immunoperoxidase nuclear staining in formalin fixed, paraffin-embedded human cerebellum tissue. -SCBT QC
Date published: 2015-05-05
Rated 5 out of 5 by from Good publishable data in WB in human MedulloblastomaGood publishable data in WB in human Medulloblastoma Cells. -SCBT Publication Review
Date published: 2015-02-19
Rated 5 out of 5 by from Robust signal in WB in SKRobust signal in WB in SK-N-SH whole cell lysate. -SCBT QC
Date published: 2015-02-17
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HuD 抗体 (E-1) is rated 4.2 out of 5 by 6.
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