Date published: 2025-9-7
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HSP 90α Antibody (F-2) - Western Blotting - Image 344052
HSP 90α 抗体 (F-2): sc-515081. HeLa (A), Y79 (B), K-562 (C), NIH/3T3 (D) 和 KNRK (E) 全细胞裂解液中 HSP 90α 表达的 Western 印迹分析.
HSP 90α 抗体 (F-2): sc-515081. HeLa (A), Y79 (B), K-562 (C), NIH/3T3 (D) 和 KNRK (E) 全细胞裂解液中 HSP 90α 表达的 Western 印迹分析.

HSP 90α 抗体 (F-2): sc-515081

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说明书
  • HSP 90 alpha 抗体 F-2 是小鼠单克隆 IgG1 κ，HSP 90α抗体, 在18篇文献中引用，规格为200 µg/ml
  • 免疫原氨基酸序列342-382位于human物种的HSP 90α的内部区域内
  • HSP 90 alpha 抗体 (F-2) 推荐用于 WB, IP, IF, IHC(P) 和 ELISA，检测mouse, rat 和human 来源的 HSP 90α
  • 抗HSP 90 alpha抗体（F-2）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取HSP 90α (F-2): sc-515081的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP 和 m-IgG1 BP-HRP 是 HSP 90α 抗体 (F-2) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与HSP 90α 抗体 (F-2) 组合成套装提供。 (请参阅下面的订购信息)。

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描述
基因信息
说明书与实验方案
研究信息

関連項目

此外 HSP 抗体 包括 HSC 70, HSP 10, HSP 105, HSP 20, HSP 27, HSP 40, HSP 47, HSP 56, HSP 60和HSP 65

HSP 90α抗体（F-2）是一种小鼠单克隆IgG1 kappa轻链抗体，可通过蛋白质印迹（WB）、免疫沉淀（IP）、免疫荧光（IF）、免疫组织化学和酶联免疫吸附测定（ELISA）检测小鼠、大鼠和人类来源的HSP 90α蛋白 ）。抗HSP 90α抗体（F-2）有非结合型和多种结合型，包括琼脂糖、辣根过氧化物酶（HRP）、藻红蛋白（PE）、异硫氰酸荧光素（FITC）和多种Alexa Fluor®结合物。HSP 90α蛋白作为分子伴侣发挥着至关重要的作用，它协助包括类固醇激素受体和激酶在内的众多客户蛋白进行正确折叠和稳定，这些蛋白对于细胞信号传导和应激反应至关重要。这种伴侣活动对于在热休克或氧化应激等应激条件下维持细胞稳态尤为重要，HSP 90α有助于防止其他蛋白聚集和错误折叠。此外，HSP 90α还与各种辅助伴侣蛋白（如p60/stip1和p23）形成复合物，进一步调节活性和客户蛋白的相互作用。 HSP 90α调节关键信号蛋白的能力凸显了其在癌症生物学中的重要性，因为许多致癌蛋白的稳定性和功能都依赖于HSP 90α，这使得该蛋白成为治疗干预的潜在靶标。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

HSP 90α 抗体 (F-2) 参考文献:

  1. PKC epsilon 是热休克反应中 hsp90 beta 基因的独特调节因子。  |  Wu, JM., et al. 2003. J Biol Chem. 278: 51143-9. PMID: 14532285
  2. HSP90 与癌症的伴侣作用。  |  Whitesell, L. and Lindquist, SL. 2005. Nat Rev Cancer. 5: 761-72. PMID: 16175177
  3. Hsp90 促进新性状的快速进化：多种真菌的抗药性。  |  Cowen, LE. and Lindquist, S. 2005. Science. 309: 2185-9. PMID: 16195452
  4. 通过可逆乙酰化调节分子伴侣 Hsp90 的功能。  |  Aoyagi, S. and Archer, TK. 2005. Trends Cell Biol. 15: 565-7. PMID: 16199163
  5. 人类基因组中的 HSP90 基因家族：对其分化和进化的见解。  |  Chen, B., et al. 2005. Genomics. 86: 627-37. PMID: 16269234
  6. Hsp90：蛋白质折叠和基因调控的伴侣。  |  Zhao, R. and Houry, WA. 2005. Biochem Cell Biol. 83: 703-10. PMID: 16333321
  7. Hsp90 调节仙台病毒感染细胞中干扰素调节因子 3 的激活和 TBK-1 的稳定。  |  Yang, K., et al. 2006. Mol Biol Cell. 17: 1461-71. PMID: 16394098
  8. 底物从伴侣 Hsp70 转移到 Hsp90。  |  Wegele, H., et al. 2006. J Mol Biol. 356: 802-11. PMID: 16403523
  9. 新生物素对 Hsp90 C 端结构域的伴侣功能和辅助伴侣相互作用的调节：香豆素类抗生素破坏 Hsp90 二聚化的证据。  |  Allan, RK., et al. 2006. J Biol Chem. 281: 7161-71. PMID: 16421106

订购信息

产品名称产品编号规格价格数量收藏夹

HSP 90α 抗体 (F-2)

sc-515081
200 µg/ml
$316.00

HSP 90α (F-2): m-IgG Fc BP-HRP 套装

sc-527978
200 µg Ab; 10 µg BP
$354.00

HSP 90α (F-2): m-IgG1 BP-HRP 套装

sc-533351
200 µg Ab; 20 µg BP
$354.00

HSP 90α 抗体 (F-2) AC

sc-515081 AC
500 µg/ml, 25% agarose
$416.00

HSP 90α 抗体 (F-2) HRP

sc-515081 HRP
200 µg/ml
$316.00

HSP 90α 抗体 (F-2) FITC

sc-515081 FITC
200 µg/ml
$330.00

HSP 90α 抗体 (F-2) PE

sc-515081 PE
200 µg/ml
$343.00

HSP 90α 抗体 (F-2) Alexa Fluor® 488

sc-515081 AF488
200 µg/ml
$357.00

HSP 90α 抗体 (F-2) Alexa Fluor® 546

sc-515081 AF546
200 µg/ml
$357.00

HSP 90α 抗体 (F-2) Alexa Fluor® 594

sc-515081 AF594
200 µg/ml
$357.00

HSP 90α 抗体 (F-2) Alexa Fluor® 647

sc-515081 AF647
200 µg/ml
$357.00

HSP 90α 抗体 (F-2) Alexa Fluor® 680

sc-515081 AF680
200 µg/ml
$357.00

HSP 90α 抗体 (F-2) Alexa Fluor® 790

sc-515081 AF790
200 µg/ml
$357.00

Mouse anti-HSP90a/b Santa Cruz Biotech Cat. #sc-13119; RRID: AB_675659. Is this helpful for qPCR analysis? Any study is available?

Asked by: Shefa
Thank you for your question. This antibody has not been validated for qPCR .
Answered by: BlakeJ
Date published: 2024-03-25

What is the total volume provided of HSP 90α (F-2):sc-515081 monoclonal antibody?

Asked by: jenni
Thank you for your question. This antibody is provided as 200 µg in 1 ml total volume of PBS with < 0.1% sodium azide and 0.1% gelatin.
Answered by: Technical Support 7
Date published: 2017-01-13

Can HSP 90α (F-2): sc-515081 monoclonal antibody be used to stain formalin-fixed, paraffin-embedded (FFPE) tissue sections?

Asked by: jenni
Thank you for your question. Yes, HSP 90α (F-2): sc-515081 has been validated for use in IHC with paraffin-embedded sections. We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support 7
Date published: 2017-01-13
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Rated 1 out of 5 by from It doesn't functionI requested a sample to try this anti-HSP 90 antibody but unfortunately, it didn't function on my protein extract.
Date published: 2023-07-31
Rated 4 out of 5 by from Detection of hsp90 by Western blottingThe antibody sc-515081 is suitable to detect hsp90 by Western blotting (minigels, SDS-denaturing PA-gels, Tris-glycine for blotting) for human and other mammalian species. There is no or minimal background staining when the analysis is being performed with standard ECL.
Date published: 2020-04-09
Rated 5 out of 5 by from Great with western blottingWe received a sample of this antibody and it worked great with total mouse brain lysate in western blotting.
Date published: 2018-11-17
Rated 5 out of 5 by from Superb !!Very clear western blot data with strong signal; also great IHC staining
Date published: 2017-01-20
Rated 5 out of 5 by from great for IHCThis HSP90 alpha antibody shows great IHC staining and good results in WB too.
Date published: 2017-01-12
Rated 4 out of 5 by from WorkedHSP 90 expression in CD34+cells was identified by this antibody, although there some backgroud, the main band is obvious.
Date published: 2016-11-22
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HSP 90α 抗体 (F-2) is rated 4.0 out of 5 by 6.
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