Date published: 2025-9-7
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HSP90 Antibody (4F10) - Western Blotting - Image 31409
HSP 90 抗体 (4F10): sc-69703. 非转染: sc-117752 (A) 和人 HSP 90 转染: sc-114003 (B) 293T 全细胞裂解液中 HSP 90 表达的 Western 印迹分析.
HSP90 Antibody (4F10) - Western Blotting - Image 31409
HSP 90 抗体 (4F10): sc-69703. 非转染: sc-117752 (A) 和人 HSP 90 转染: sc-114003 (B) 293T 全细胞裂解液中 HSP 90 表达的 Western 印迹分析.
HSP90 Antibody (4F10) - Western Blotting - Image 54296
HSP 90 抗体 (4F10): sc-69703. 未转染 293T: sc-117752 (A), 转染人 HSP 90 的 293T: sc-114003 (B) 和 HeLa (C) 全细胞裂解液中 HSP 90 表达的 Western 印迹分析.
HSP90 Antibody (4F10) - Western Blotting - Image 412664
HSP90 抗体 (4F10)：sc-69703。K-562 (A) 和 HCT-116 (B) 细胞裂解液中 HSP 90 表达的 Western 印迹分析。所用检测试剂：m-IgG Fc BP-HRP：sc-525409。
HSP 90 抗体 (4F10): sc-69703. 非转染: sc-117752 (A) 和人 HSP 90 转染: sc-114003 (B) 293T 全细胞裂解液中 HSP 90 表达的 Western 印迹分析.

HSP90 抗体 (4F10): sc-69703

4.8(19)
寫評論提問

说明书
  • HSP90 抗体 4F10 是小鼠单克隆 IgG2b，HSP90抗体, 在157篇文献中引用，规格为50 µg/0.5 ml
  • 针对全长重组HSP 90，human起源
  • 推荐用于 human 来源的HSP 90 WB, IP 和 ELISA检测
  • 看 HSP90 alpha/beta (F-8): sc-13119，可查看有标记的 HSP90 抗体，包括AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 和 790
  • 关于如何获取HSP90 (4F10): sc-69703的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • 2b BP-HRP">m-IgG2b BP-HRP是HSP90 Antibody (4F10) 适用于 WB 应用。 的首选辅助检测试剂。该试剂现与HSP90 Antibody (4F10) 打包提供（请参阅下面的订购信息）。

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描述
基因信息
说明书与实验方案
研究信息

関連項目

额外HSP抗体，包括HSC 70, HSP 10, HSP 105, HSP 20, HSP 27, HSP 40, HSP 47, HSP 56, HSP 60, HSP 65, HSP 70, HSP 75, HSP 90, HSP 90α, HSP 90β和HSP 40-4

HSP 90抗体（4F10）是一种IgG2b小鼠单克隆HSP 90抗体（也称为HSP90抗体、HSP90AA1抗体或HSP90AB1抗体），可通过WB、IP和ELISA检测人源HSP 90蛋白。HSP 90抗体（4F10）可作为非偶联抗HSP 90抗体使用。热休克反应首次在果蝇唾液腺细胞中被描述，形态上由其多线染色体膨出模式的改变组成，涉及少数蛋白质的从头合成。后来在细菌鸡和哺乳动物细胞中发现了类似的热休克蛋白，随后在其他生物中进行了研究。一系列蛋白质，包括HSP 90、HSP 70、HSP 20-30和泛素，是由温度休克、化学物质和其他环境应激等损伤引起的。HSP 90和其他HSPs的主要功能是作为分子伴侣。HSP 90与糖皮质激素受体（GR）形成复合物，使非配体结合受体在转录上失活。HSP 90作为由HSP 56或亲环素D组成的异源复合物与GR结合，形成无受体复合物。HSP 90还以二聚体的形式与其他蛋白质如p60/STI1和p23存在，与雌激素和雄激素受体形成无受体复合物。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

HSP90 抗体 (4F10) 参考文献:

  1. PKC epsilon 是热休克反应中 hsp90 beta 基因的独特调节因子。  |  Wu, JM., et al. 2003. J Biol Chem. 278: 51143-9. PMID: 14532285
  2. HSP90 与癌症的伴侣作用。  |  Whitesell, L. and Lindquist, SL. 2005. Nat Rev Cancer. 5: 761-72. PMID: 16175177
  3. Hsp90 促进新性状的快速进化：多种真菌的抗药性。  |  Cowen, LE. and Lindquist, S. 2005. Science. 309: 2185-9. PMID: 16195452
  4. 通过可逆乙酰化调节分子伴侣 Hsp90 的功能。  |  Aoyagi, S. and Archer, TK. 2005. Trends Cell Biol. 15: 565-7. PMID: 16199163
  5. 人类基因组中的 HSP90 基因家族：对其分化和进化的见解。  |  Chen, B., et al. 2005. Genomics. 86: 627-37. PMID: 16269234
  6. Hsp90：蛋白质折叠和基因调控的伴侣。  |  Zhao, R. and Houry, WA. 2005. Biochem Cell Biol. 83: 703-10. PMID: 16333321
  7. Hsp90 调节仙台病毒感染细胞中干扰素调节因子 3 的激活和 TBK-1 的稳定。  |  Yang, K., et al. 2006. Mol Biol Cell. 17: 1461-71. PMID: 16394098
  8. 底物从伴侣 Hsp70 转移到 Hsp90。  |  Wegele, H., et al. 2006. J Mol Biol. 356: 802-11. PMID: 16403523
  9. 新生物素对 Hsp90 C 端结构域的伴侣功能和辅助伴侣相互作用的调节：香豆素类抗生素破坏 Hsp90 二聚化的证据。  |  Allan, RK., et al. 2006. J Biol Chem. 281: 7161-71. PMID: 16421106

订购信息

产品名称产品编号规格价格数量收藏夹

HSP90 抗体 (4F10)

sc-69703
50 µg/0.5 ml
RMB2377.00

HSP90 (4F10): m-IgG2b BP-HRP 套装

sc-548446
50 µg Ab; 10 µg BP
RMB2662.00

Does the antibody bind to the n-terminal ATP binding site of HSP90?

Asked by: Anonymous
Thank you for your question. The exact epitope recognized by this mouse monoclonal antibody has not yet been determined.
Answered by: Technical Support
Date published: 2020-07-26

Hello, I was wondering what are two bands presented on your Western image. Thanks

Asked by: Sanja
Thank you for your question. The Western blot data you are referring to is in a human HSP 90 transfected 293T whole cell lysate. This data is not representative of endogenous protein expression, and the multiple bands observed may be the result of incomplete products of translation. The expected molecular weight for endogenous HSP 90 is ~ 90 kDa, and if multiple bands are observed this may also be due to post-translational modifications of the protein.
Answered by: Technical Support
Date published: 2020-03-11

Can this antibody be used for mouse Hsp90 protein in western blotting?

Asked by: Arundhati
Thank you for your question. This antibody has not been tested for mouse use, and can therefore not be warrantied for this use. If you have any further questions, please contact Technical Service.
Answered by: Tech Service
Date published: 2019-05-24

This is Arun from india i am a research scholar and I am interested to buy this antibody before that I would like to test this antibody so how can i apply for free sample?

Asked by: Arun
Thank you for your question. We are not currently offering this specific antibody, HSP 90 (4F10): sc-69703, in a free sample size. As a possible alternative, we would recommend either our, HSP 90α (F-2): sc-515081, or our HSP 90α/β (F-8): sc-13119, both of which are available in a free sample size. Please contact one of our authorized distributors in India to request a sample. https://www.scbt.com/scbt/resources/distributors
Answered by: Technical Support
Date published: 2017-08-18

What is the recommended secondary reagent for detection of HSP 90 Antibody (4F10) via Western blot?

Asked by: Trav11
We recommend using one of our exclusive Mouse IgG Binding Proteins as a secondary detection reagent, such as m-IgGκ BP-HRP: sc-516102, for Western blot. A complete list of all available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Answered by: Tech Service 8
Date published: 2016-12-20

Would it be possible for this antibody to work in avian samples?

Asked by: Trav11
To check additional species reactivity not listed on our website, please contact our Technical Service department at 800-457-3801 and we will be more than happy to run sequence analysis for your species of interest.
Answered by: Tech Service 8
Date published: 2016-12-20
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Rated 5 out of 5 by from WBできれいに検出できました抗体はWBに使用しました。サンプルはJurkatとMT-2細胞株。抗体の希釈倍率は1000倍。ブロッキング剤は市販のブロッキング剤または5%スキムミルクを使用しました。どちらのブロッキング剤を使用してもバックグラウンドはかなり低く、きれいなバンドが検出されました。
Date published: 2022-05-10
Rated 4 out of 5 by from HSP 90good antibody for Western blot, very clean for human cell extracts.
Date published: 2020-01-22
Rated 5 out of 5 by from GOODAntibody showed perfect bands in western blotting.
Date published: 2019-10-16
Rated 5 out of 5 by from Excellent antibody!!!Very strong bands in Western blot in 1:250 dilution, and also in 1:500 dilution, in mouse tumor tissues. Thank you santa cruz!
Date published: 2018-05-11
Rated 5 out of 5 by from Great antibody for western blotThe batch of antibody I have worked well on BJAB, HeLa, MCF7, NIH/3T3, Jurkat and K562 total cell extracts for western Blot. The band is specific. The antibody was diluted 200 times into 5% BSA, TBST and incubated over-night at 4°C.
Date published: 2018-01-04
Rated 4 out of 5 by from HSP 90 Antibody (4F10) ReviewMouse anti-HSP 90 Antibody (4F10) SAMPLE works verywell in WB at 1:1000 in 5 % BSA with LN229 cells and can be reused. And the bands were clear.
Date published: 2017-12-27
Rated 5 out of 5 by from Effect is goodUsed this antibody for several times, the effect were good. Mainly used in prostate cancer cells, 1:100 start, sample volume of 60 micrograms. High cost performance.
Date published: 2017-02-14
Rated 5 out of 5 by from strong and clearWestern blot strong and clear signal was got, by using HL-60, gingival fibroblast (hGF) cell and PBMC samples.
Date published: 2017-01-11
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HSP90 抗体 (4F10) is rated 4.8 out of 5 by 19.
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