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HSP 60 Antibody (LK1) - Western Blotting - Image 353947
HSP 60 抗体 (LK1): sc-59567. KNRK (A) 和 PC-12 (B) 全细胞裂解液以及小鼠胎盘 (C) 和人类肾脏 (D) 组织提取物中 HSP 60 表达的 Western 印迹分析.
HSP 60 抗体 (LK1): sc-59567. KNRK (A) 和 PC-12 (B) 全细胞裂解液以及小鼠胎盘 (C) 和人类肾脏 (D) 组织提取物中 HSP 60 表达的 Western 印迹分析.

HSP 60 抗体 (LK1): sc-59567

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说明书
  • HSP 60 抗体 LK1 是小鼠单克隆 IgG1 κ，HSP 60抗体, 在37篇文献中引用，规格为200 µg/ml
  • 针对human起源的重组HSP 60，其表位与氨基酸383-447对应
  • HSP 60 抗体 (LK1) 推荐用于 WB, IP, IF, IHC(P), FCM 和 ELISA，检测mouse, rat, human, bovine, porcine 和canine 来源的 only the mammalian (not bacterial) HSP 60
  • 抗HSP 60抗体（LK1）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取HSP 60 (LK1): sc-59567的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP, m-IgG1 BP-HRP 和 m-IgGκ BP-HRP 是 HSP 60 抗体 (LK1) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与HSP 60 抗体 (LK1) 组合成套装提供。 (请参阅下面的订购信息)。

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描述
基因信息
说明书与实验方案
研究信息

関連項目

此外 HSP 抗体 包括 HSC 70, HSP 10, HSP 105, HSP 20, HSP 27, HSP 40, HSP 47, HSP 56, HSP 60和HSP 65

HSP 60 抗体（LK1）是一种小鼠单克隆 IgG1 kappa 轻链抗体，可通过蛋白质印迹（WB）、免疫沉淀（IP）、免疫荧光（IF）、石蜡包埋切片免疫组织化学（IHCP）、流式细胞术（FCM）和酶联免疫吸附测定（ELISA）检测小鼠、大鼠、人、牛、猪和犬的 HSP 60 蛋白。抗HSP 60抗体（LK1）有非结合型和多种结合型，包括琼脂糖、辣根过氧化物酶（HRP）、藻红蛋白（PE）、异硫氰酸荧光素（FITC）和多种Alexa Fluor®结合物。HSP 60（又称热休克蛋白60）通过协助新合成的蛋白质正确折叠并组装到线粒体中，在线粒体功能中发挥着至关重要的作用。这个过程可以保持线粒体的完整性和功能，因为错误折叠的蛋白质会导致线粒体功能障碍，并导致包括神经退行性疾病和代谢综合征在内的各种疾病。HSP 60参与免疫反应，作为伴侣蛋白影响免疫细胞的激活。HSP 60与其他蛋白质（如HSP 10）的相互作用进一步凸显了HSP 60在细胞稳态和应激反应中的重要性。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

HSP 60 抗体 (LK1) 参考文献:

  1. 人类HSP 60肽反应性T细胞系在白塞氏病患者和健康对照中同样存在。  |  Saruhan-Direskeneli, G., et al. 2001. Immunol Lett. 79: 203-8. PMID: 11600199
  2. 白塞氏病皮肤粘膜病变的HSP 60表达。  |  Ergun, T., et al. 2001. J Am Acad Dermatol. 45: 904-9. PMID: 11712037
  3. 不育夫妇男性伴侣中的人类 60kd 热休克蛋白（HSP 60）精液抗体。  |  Eggert-Kruse, W., et al. 2002. Hum Reprod. 17: 726-35. PMID: 11870127
  4. 对患有沙眼衣原体相关输卵管因素不孕症的妇女进行TNF-α和HSP-60的免疫组织化学分析。  |  Zhao, H. and Li, H. 2004. J Huazhong Univ Sci Technolog Med Sci. 24: 630-2. PMID: 15791863
  5. γδT淋巴细胞亚群识别单个hsp-60表位。  |  O'Brien, RL., et al. 1991. Immunol Rev. 121: 155-70. PMID: 1718844
  6. 在实验性自身免疫性青光眼大鼠模型中，HSP 60免疫后血清自身抗体反应性的增强。  |  Joachim, SC., et al. 2010. Curr Eye Res. 35: 900-8. PMID: 20858111
  7. 冠心病患者外周血中可检测到 OxLDL- 和 HSP-60 抗原特异性 CD8（+）T 淋巴细胞  |  Ghio, M., et al. 2013. Clin Exp Med. 13: 251-5. PMID: 22886609
  8. 作为缺氧诱导因子（HIF）-1 转录活性和热休克蛋白（HSP）60 合子活性抑制剂的原碳硼酰苯氧基乙酰苯胺。  |  Li, G., et al. 2015. Bioorg Med Chem Lett. 25: 2624-8. PMID: 25981686
  9. 卵巢癌患者存活与60 kD热休克蛋白HSP-60的mRNA表达的相关性。  |  Kimura, E., et al. 1993. J Clin Oncol. 11: 891-8. PMID: 8098058
  10. 分枝杆菌60,000 MW热休克蛋白（hsp 60）的HLA-DR4限制性T细胞表位与人类hsp 60的序列同源区域不匹配。  |  Mustafa, AS., et al. 1996. Immunology. 87: 421-7. PMID: 8778028

订购信息

产品名称产品编号规格价格数量收藏夹

HSP 60 抗体 (LK1)

sc-59567
200 µg/ml
RMB2377.00

HSP 60 (LK1): m-IgG Fc BP-HRP 套装

sc-528636
200 µg Ab; 10 µg BP
RMB2662.00

HSP 60 (LK1): m-IgGκ BP-HRP 套装

sc-521066
200 µg Ab, 40 µg BP
RMB2662.00

HSP 60 (LK1): m-IgG1 BP-HRP 套装

sc-543043
200 µg Ab; 20 µg BP
RMB2662.00

HSP 60 抗体 (LK1) AC

sc-59567 AC
500 µg/ml, 25% agarose
RMB3129.00

HSP 60 抗体 (LK1) HRP

sc-59567 HRP
200 µg/ml
RMB2377.00

HSP 60 抗体 (LK1) FITC

sc-59567 FITC
200 µg/ml
RMB2482.00

HSP 60 抗体 (LK1) PE

sc-59567 PE
200 µg/ml
RMB2580.00

HSP 60 抗体 (LK1) Alexa Fluor® 488

sc-59567 AF488
200 µg/ml
RMB2685.00

HSP 60 抗体 (LK1) Alexa Fluor® 546

sc-59567 AF546
200 µg/ml
RMB2685.00

HSP 60 抗体 (LK1) Alexa Fluor® 594

sc-59567 AF594
200 µg/ml
RMB2685.00

HSP 60 抗体 (LK1) Alexa Fluor® 647

sc-59567 AF647
200 µg/ml
RMB2685.00

HSP 60 抗体 (LK1) Alexa Fluor® 680

sc-59567 AF680
200 µg/ml
RMB2685.00

HSP 60 抗体 (LK1) Alexa Fluor® 790

sc-59567 AF790
200 µg/ml
RMB2685.00

Dear Sirs, can we have information on the double band of Hsp60 found with the Hsp60 LK1 mouse antibody, which we see in some Wb shown here? Thanks

Asked by: Federica
Thank you for your question. HSP 60 is subject to crosslinking and a multitude of post-translational modifications, which may contribute to observable molecular weight in western blot. Please contact our technical service team directly if you require additional information or troubleshooting advice.
Answered by: Tech Support Europe
Date published: 2024-09-19

Can HSP 60 (LK1): sc-59567 monoclonal antibody be used in IF or IHC with mouse tissue or cells? Will there be non-specific staining?

Asked by: Randy McDonald
Thank you for your inquiry. The use of mouse monoclonal antibodies with mouse samples is very common and typically poses no problem. To eliminate any potential cross-reactivity of an anti-mouse secondary detection reagent, HSP 60 (LK1): sc-59567 is available in a variety of direct conjugations, such as HRP, PE, FITC and AlexaFluors.
Answered by: Technical Support
Date published: 2017-02-24
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Rated 4 out of 5 by from Good antibodyUsing Immunohistochemistry of free floating samples (DAB method) of rat brain hippocampus, we got good signal.
Date published: 2020-12-29
Rated 4 out of 5 by from Anticorps validéTest de cet anticorps sur lignée humaine (kératinocyte et myoblaste) en cytomètrie en flux -> validé
Date published: 2018-03-21
Rated 5 out of 5 by from Worked pretty wellI got a free sample like 2-3 weeks ago. It worked really well in my cells. I used 1:500 for WB and the bands are clean and clear.
Date published: 2018-01-30
Rated 5 out of 5 by from strong and specific bandI tried this antibody at 1:1,000 dilution, works well.
Date published: 2017-11-02
Rated 4 out of 5 by from Good antibodyWorked well with western blotting at 1:500 dilution.
Date published: 2017-09-27
Rated 5 out of 5 by from Great antibody against HSP 60 (LK1)We purchased this antibody last month and I used in WB to detect the location of mitochondria in cell lysates. This antibody gave a clean and single band in the correct MW.
Date published: 2017-09-10
Rated 5 out of 5 by from Good signal in WBBand at the expected size in western blot for murine neuronal cell line lysate.
Date published: 2017-09-01
Rated 5 out of 5 by from suitable for WBwestern blot of A2780 cell lysate using 1:1000 dilution. Good choice !
Date published: 2017-08-26
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HSP 60 抗体 (LK1) is rated 4.4 out of 5 by 19.
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