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HSP 60 Antibody (H-1) - Western Blotting - Image 354581
HSP 60 抗体 (H-1): sc-13115. Jurkat (A), HEK293T (B), F9 (C) 和 K-562 (D) 全细胞裂解液及人肾组织提取物 (E) 中 HSP 60 表达的 Western 印迹分析.
HSP 60 抗体 (H-1): sc-13115. Jurkat (A), HEK293T (B), F9 (C) 和 K-562 (D) 全细胞裂解液及人肾组织提取物 (E) 中 HSP 60 表达的 Western 印迹分析.

HSP 60 抗体 (H-1): sc-13115

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寫評論提問

说明书
  • HSP 60抗体(H-1)是小鼠单克隆IgG3 κ, 在162篇文献中引用，规格为200 µg/ml
  • 特异性抗原位于human物种的HSP 60的N-terminus附近的氨基酸31-50之间
  • 推荐用于 mouse, rat， human 和 来源的HSP 60 WB, IP, IF, IHC(P) 和 ELISA检测; 也和以下物种反应，包括： 和 equine, canine, bovine, porcine and avian
  • 看 HSP 60 (LK1): sc-59567，可查看有标记的 HSP 60 抗体，包括AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 和 790
  • 关于如何获取HSP 60 (H-1): sc-13115的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG3 BP-HRP是HSP 60 Antibody (H-1) 适用于 WB 和 IHC(P) 应用。 的首选辅助检测试剂，该试剂现与HSP 60 Antibody (H-1) 搭配使用（请参阅下面的订购信息）。

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描述
基因信息
说明书与实验方案
研究信息

関連項目

额外HSP抗体，包括HSC 70, HSP 10, HSP 105, HSP 20, HSP 27, HSP 40, HSP 47, HSP 56, HSP 60, HSP 65, HSP 70, HSP 75, HSP 90, HSP 90α, HSP 90β和HSP 40-4

HSP 60 抗体（H-1）是一种小鼠单克隆 IgG3 kappa 轻链抗体，可通过蛋白质印迹（WB）、免疫沉淀（IP）、免疫荧光（IF）、免疫组织化学和酶联免疫吸附测定（ELISA）等方法检测小鼠、大鼠和人类样本中的 HSP 60。抗HSP 60抗体（H-1）以非结合形式提供，可用于多种实验。HSP 60是热休克蛋白家族的重要成员，通过协助新合成的蛋白质正确折叠和组装，在线粒体功能中发挥关键作用。这个过程维持细胞内环境稳定，确保蛋白质达到功能构象，这对细胞代谢和能量产生至关重要。HSP 60还参与免疫反应，作为细胞表面标记物，帮助γ/δ T细胞识别受压或受损细胞，将细胞应激反应与免疫监控联系起来。HSP 60在线粒体和细胞表面具有双重功能，凸显其在细胞功能和免疫调节中的重要性，使HSP 60单克隆抗体（H-1）成为研究这些关键生物过程的宝贵研究工具。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

HSP 60 抗体 (H-1) 参考文献:

  1. 暴露于致死和亚致死浓度亚砷酸钠的培养人尿道细胞（UROtsa）中 hsp 27、hsp 60、hsc 70 和 hsp 70 应激反应基因的表达。  |  Rossi, MR., et al. 2002. Environ Health Perspect. 110: 1225-32. PMID: 12460802
  2. hsp60 的抗折叠活性将蛋白质导入线粒体基质与导出到膜间隙相结合。  |  Koll, H., et al. 1992. Cell. 68: 1163-75. PMID: 1347713
  3. 伴侣素 Hsp60 防止蛋白质在热应力下变性  |  Martin, J., et al. 1992. Science. 258: 995-8. PMID: 1359644
  4. 热休克蛋白 Hsp60 反应性 gamma delta 细胞：具有高度集中特异性的大型多样化 T 淋巴细胞亚群。  |  O'Brien, RL., et al. 1992. Proc Natl Acad Sci U S A. 89: 4348-52. PMID: 1584768
  5. 血清抗体对HSP 60和HSP 70的诊断意义。  |  Bleotu, C., et al. 2014. Virulence. 5: 828-31. PMID: 25483863
  6. 淫羊藿素对缺氧缺糖诱导损伤的PC12细胞中HIF-1α、HSP-60和HSP-70表达的影响。  |  Mo, ZT., et al. 2017. Pharm Biol. 55: 848-852. PMID: 28140748
  7. 线粒体伴侣 HSP-60 通过 p38 MAP 激酶信号调节抗菌免疫力。  |  Jeong, DE., et al. 2017. EMBO J. 36: 1046-1065. PMID: 28283579
  8. 热休克蛋白，重点介绍HSP 60。  |  Malik, JA. and Lone, R. 2021. Mol Biol Rep. 48: 6959-6969. PMID: 34498161
  9. 温度变化后大肠杆菌中单个多肽的瞬时合成率。  |  Lemaux, PG., et al. 1978. Cell. 13: 427-34. PMID: 350413
  10. 氨基酸类似物和热休克对鸡胚成纤维细胞基因表达的影响。  |  Kelley, PM. and Schlesinger, MJ. 1978. Cell. 15: 1277-86. PMID: 569556
  11. 人类外周γ-δT 细胞可识别 Daudi Burkitt 淋巴瘤细胞上的 hsp60 分子。  |  Kaur, I., et al. 1993. J Immunol. 150: 2046-55. PMID: 8094731
  12. 人类hsp 60基因的遗传复杂性。  |  Pochon, NA. and Mach, B. 1996. Int Immunol. 8: 221-30. PMID: 8671607

订购信息

产品名称产品编号规格价格数量收藏夹

HSP 60 抗体 (H-1)

sc-13115
200 µg/ml
RMB2377.00

HSP 60 (H-1): m-IgG3 BP-HRP 套装

sc-550544
200 µg Ab; 40 µg BP
RMB2662.00

HSP 60 (H-1) 中和勝肽

sc-13115 P
100 µg/0.5 ml
RMB511.00

Can I use this mouse monoclonal antibody for immunohistochemical staining of mouse tissue?

Asked by: DefinitelyNotMatt
Thank you for your question. We recommend using a directly conjugated mouse monoclonal primary antibody, catalog number SC-59567. This antibody is currently available conjugated to phycoerythrin (sc-59567 PE), fluorescein (sc-59567 FITC), Alexa Fluor® 488 (sc-59567 AF488) or Alexa Fluor® 647 (sc-59567 AF647). Please contact Technical Service if you have further questions concerning this product.
Answered by: Technical Support 12
Date published: 2017-01-10
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Rated 3 out of 5 by from Discreto funzionamentoHo effettuato un solo esperimento prova per valutare la reattività nell'equino e ha funzionato, ma vanno sicuramente migliorate le condizioni di ibridazione
Date published: 2023-07-14
Rated 4 out of 5 by from Segnale molto forteSono soddisfatta di questo anticorpo, l'ho usato su lisati totali di precursori neurali umani trattati con CCCP a diverse concentrazioni e diversi tempi. Ho avuto un segnale della proteina molto evidente già a pochi minuti.
Date published: 2022-05-20
Rated 4 out of 5 by from Good antibody sensitivityGood antibody sensitivity. Fast visualization and calculation process.
Date published: 2021-04-27
Rated 4 out of 5 by from GoodIt worked well in Hs578t and MDA-MB-157 cell lines ( 2% milk-1:1000).
Date published: 2017-10-31
Rated 5 out of 5 by from A very good antibodyA good antibody for WB, used in sub cellular fractionation of human HeLa cells
Date published: 2017-10-03
Rated 4 out of 5 by from Good antibodyWorked well with western blotting at 1:500 dilution.
Date published: 2017-09-27
Rated 5 out of 5 by from Good signal in WBBand at the expected size in western blot for mouse neuronal cell line.
Date published: 2017-09-01
Rated 5 out of 5 by from A great choice when studying HSP 60A smart choice for you HSP 60 detection needs. As long as you are performing a recommended application, this antibody will help you get the results you desire. It has nearly 50 citations to its name.
Date published: 2017-01-06
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HSP 60 抗体 (H-1) is rated 4.6 out of 5 by 16.
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