Date published: 2025-9-6

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hnRNP U 抗体 (3G6): sc-32315

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说明书
  • hnRNP U 抗体 3G6 是小鼠单克隆 IgG1 κ,hnRNP U抗体, 在52篇文献中引用,规格为200 µg/ml
  • 针对human起源的hnRNP U全长重组蛋白
  • hnRNP U 抗体 (3G6) 推荐用于 WB, IP, IF, IHC(P) 和 ELISA,检测mouse, rat 和human 来源的 hnRNP U
  • 抗hnRNP U抗体(3G6)可与琼脂糖结合用于IP;与HRP结合用于WB、IHC(P)和ELISA;与藻红蛋白FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取hnRNP U (3G6): sc-32315免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRPm-IgGκ BP-HRP 是 hnRNP U 抗体 (3G6) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与hnRNP U 抗体 (3G6) 组合成套装提供。 (请参阅下面的订购信息)。

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関連項目

hnRNP U抗体(3G6)是一种小鼠单克隆IgG1 kappa轻链抗体,可通过蛋白质印迹(WB)、免疫沉淀(IP)、免疫荧光(IF)、石蜡包埋切片免疫组织化学(IHCP)和酶联免疫吸附测定(ELISA)检测小鼠、大鼠和人类来源的hnRNP U蛋白。抗hnRNP U抗体 (3G6)有非结合型和多种结合型,包括琼脂糖、辣根过氧化物酶(HRP)、藻红蛋白(PE)、异硫氰酸荧光素(FITC)和多种Alexa Fluor®结合物。hnRNP U蛋白,也称为HNRNPU、GRIP120或p120, 通过参与前mRNA处理和运输,在调节基因表达方面发挥着至关重要的作用。hnRNP U蛋白主要位于核质中,与RNA聚合酶II转录物相互作用,促进异质核RNA(hnRNA)的成熟。hnRNP U蛋白在核质中的定位能够有效处理和运输前mRNA,这对于正常的基因表达和细胞功能至关重要 。hnRNP U蛋白与DNA的支架附着区域结合,影响染色质结构和基因可及性。hnRNP U蛋白通过干扰RNA聚合酶II羧基末端域的磷酸化来抑制RNA聚合酶的延伸,凸显了hnRNP U蛋白在转录调控中的重要性。hnRNP U蛋白与糖皮质激素受体的相互作用表明其在调节各种细胞过程的激素反应中起着关键作用。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

hnRNP U 抗体 (3G6) 参考文献:

  1. hnRNP U 可抑制 TFIIH 的羧基末端结构域磷酸化,并抑制 RNA 聚合酶 II 的延伸。  |  Kim, MK. and Nikodem, VM. 1999. Mol Cell Biol. 19: 6833-44. PMID: 10490622
  2. 一氧化氮供体诱导 HL-60 细胞凋亡过程中的 Caspase 激活和细胞色素 c 释放。  |  Yabuki, M., et al. 2000. Free Radic Res. 32: 507-14. PMID: 10798716
  3. hnRNP U 蛋白的一级结构和结合活性:通过 RGG 框结合 RNA。  |  Kiledjian, M. and Dreyfuss, G. 1992. EMBO J. 11: 2655-64. PMID: 1628625
  4. 在人体细胞中,hnRNP-U/SAF-A 由两种不同多聚腺苷酸化的 mRNA 编码。  |  Fackelmayer, FO. and Richter, A. 1994. Biochim Biophys Acta. 1217: 232-4. PMID: 7509195
  5. 支架/基质附着区结合蛋白 hnRNP-U (SAF-A) 在体内直接与染色体 DNA 结合:一项化学交联研究。  |  Göhring, F. and Fackelmayer, FO. 1997. Biochemistry. 36: 8276-83. PMID: 9204873
  6. 糖皮质激素受体与 RNA 结合核基质蛋白 hnRNP U 有关。  |  Eggert, M., et al. 1997. J Biol Chem. 272: 28471-8. PMID: 9353307
  7. 异质核核糖核蛋白颗粒 U 与水泡性口炎病毒的领导 RNA 序列之间的特异性相互作用。  |  Gupta, AK., et al. 1998. J Virol. 72: 8532-40. PMID: 9765391
  8. 四种 hnRNP 蛋白在 HeLa 细胞间期核和核基质中与转录位点, 核斑点以及相互之间的空间组织关系。  |  Mattern, KA., et al. 1999. Exp Cell Res. 246: 461-70. PMID: 9925762

订购信息

产品名称产品编号规格价格数量收藏夹

hnRNP U 抗体 (3G6)

sc-32315
200 µg/ml
RMB2377.00

hnRNP U (3G6): m-IgG Fc BP-HRP 套装

sc-528460
200 µg Ab; 10 µg BP
RMB2662.00

hnRNP U (3G6): m-IgGκ BP-HRP 套装

sc-520843
200 µg Ab, 40 µg BP
RMB2662.00

hnRNP U 抗体 (3G6) AC

sc-32315 AC
500 µg/ml, 25% agarose
RMB3129.00

hnRNP U 抗体 (3G6) HRP

sc-32315 HRP
200 µg/ml
RMB2377.00

hnRNP U 抗体 (3G6) FITC

sc-32315 FITC
200 µg/ml
RMB2482.00

hnRNP U 抗体 (3G6) PE

sc-32315 PE
200 µg/ml
RMB2580.00

hnRNP U 抗体 (3G6) Alexa Fluor® 488

sc-32315 AF488
200 µg/ml
RMB2685.00

hnRNP U 抗体 (3G6) Alexa Fluor® 546

sc-32315 AF546
200 µg/ml
RMB2685.00

hnRNP U 抗体 (3G6) Alexa Fluor® 594

sc-32315 AF594
200 µg/ml
RMB2685.00

hnRNP U 抗体 (3G6) Alexa Fluor® 647

sc-32315 AF647
200 µg/ml
RMB2685.00

hnRNP U 抗体 (3G6) Alexa Fluor® 680

sc-32315 AF680
200 µg/ml
RMB2685.00

hnRNP U 抗体 (3G6) Alexa Fluor® 790

sc-32315 AF790
200 µg/ml
RMB2685.00

I am using the LiCor/Odyssey system. Which secondary antibody do you recommend using with sc-32315: hnRNP U (3G6)?

Asked by: cjMara
We recommend using one of our our exclusive Mouse IgG Binding Proteins (m-IgGκ) conjugated to CFL 680 or CFL 790. They are suitable for use with Near-Infrared (NIR) detection systems, such as LI-COR/Odyssey and other comparable systems. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Answered by: Technical Support 3
Date published: 2017-01-11

What is the exact epitope recognized by sc-32315: hnRNP U (3G6)?

Asked by: cjMara
hnRNP U Antibody (3G6) is a mouse monoclonal raised against recombinant full length protein of hnRNP U of human origin. The exact epitope has not been determined.
Answered by: Technical Support 3
Date published: 2017-01-11
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Rated 5 out of 5 by from It works perfectly in WBhnRNP U Antibody (3G6): sc-32315. Western blot analysis of hnRNP U expression in human Neural Progenitor cells (hNPCs) after nuclear (N) and cytoplasmic (C) fractionation.
Date published: 2021-09-03
Rated 5 out of 5 by from Immunoflorescence Review for hnRNP U AbRecently purchased this and will be purchasing more. Worked great for immunoflorescence.
Date published: 2019-01-30
Rated 4 out of 5 by from Good for WBSpecific single band is detected. No band detected for KO cell lysates.
Date published: 2018-08-29
Rated 5 out of 5 by from a fantastic antibody for WBThis antibody recognizes a clear and strong band of predicted M.W. on WB (1:2000 dilution, 40 ug HeLa extract).
Date published: 2017-09-03
Rated 5 out of 5 by from 抗体很好用申请的小样产品,在人肺鳞癌SK-MES-1细胞做了wb实验,实验结果挺好的,抗体效果不错,上样25ug做出来效果很好,无杂带,条带清晰无杂带,显影2秒钟就出条带。
Date published: 2017-03-04
Rated 5 out of 5 by from 抗体功能很好这个抗体非常好用,之前试用小样后发现很好用,我做的是免疫荧光,小鼠组织,阳性信号非常强。所以一个月前订了一支抗体。
Date published: 2017-02-24
Rated 5 out of 5 by from OutstandingGreat western data; strong, specific signal; also superb IHC
Date published: 2017-01-29
Rated 5 out of 5 by from Great antibody for IFThe hnRNP U Antibody (3G6): sc-32315 works very well for immunofluorescence showing staining localized to the nucleus.
Date published: 2017-01-13
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hnRNP U 抗体 (3G6) is rated 4.7 out of 5 by 18.
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