Date published: 2025-9-8
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HNF-3β Antibody (H-4) - Western Blotting - Image 283494
HNF-3β (H-4): sc-374376. Hep G2 (A) 和 A549 (B) 核提取物中 HNF-3β 表达的 Western 印迹分析.
HNF-3β (H-4): sc-374376. Hep G2 (A) 和 A549 (B) 核提取物中 HNF-3β 表达的 Western 印迹分析.

HNF-3β 抗体 (H-4): sc-374376

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说明书
  • HNF-3β抗体(H-4)是小鼠单克隆IgG1 κ, 在56篇文献中引用，规格为200 µg/ml
  • 特异性抗原位于mouse物种的HNF-3β的C-terminus的氨基酸431-459之间
  • 抗-HNF-3 beta 抗体 (H-4) 推荐用于 WB, IP, IF 和 ELISA，检测mouse, rat 和human 来源的 HNF-3β; 也和以下物种反应，包括： 和 equine, canine, bovine and porcine
  • 抗HNF-3 beta抗体（H-4）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 可用于凝胶迁移和ChIP的TransCruz试剂(sc-374376 X, 200 µg/0.1 ml)
  • 关于如何获取HNF-3β (H-4): sc-374376的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgGκ BP-HRP是HNF-3β Antibody (H-4) 适用于 WB 应用。 的首选辅助检测试剂。该试剂现可与HNF-3β Antibody (H-4) 搭配使用（请参阅下面的订购信息）。有关其它 m-IgGκ BP 结合物，请参阅我们完整的小鼠 IgG 结合蛋白列表。

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描述
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说明书与实验方案
研究信息

関連項目

附加HNF抗体，包括HNF-1, HNF-1 α, HNF-1 β, HNF-3 α, HNF-3 β, HNF-3 γ, HNF-4 α和HNF-6

HNF-3β抗体（H-4）是一种IgG1 κ小鼠单克隆HNF-3β抗体（也称为HNF-3β抗体或FOXA2抗体），能够通过WB、IP、IF和ELISA技术检测小鼠、大鼠和人类来源的HNF-3β。HNF-3β抗体（H-4）既以非偶联的抗HNF-3β抗体形式提供，也以多种偶联形式的抗HNF-3β抗体提供，包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®偶联物。HNF-1（α和β）、HNF-3（α、β和γ）、HNF-4（α和γ）以及HNF-6部分组成了一个被称为肝细胞核因子家族的同蛋白家族。各种HNF-1异构体调节肝脏以及其他组织（如肾脏、小肠和胸腺）中的基因转录。HNF-3α、HNF-3β和HNF-3γ调节成年肝脏中众多肝细胞基因的转录。HNF-3α和HNF-3β还参与原肠胚形成事件，如体轴形成。HNF-4α和HNF-4γ对早期胚胎发育很重要。HNF-4α在肝脏、肾脏、胰腺、小肠、睾丸和结肠中表达；HNF-4γ在这些组织中除肝脏外均有表达。已显示HNF-6与HNF-3β的启动子结合，这表明HNF-6在肠内胚层上皮细胞分化中的潜在作用。有证据表明，HNF-6也可能是至少22个其他肝细胞富集基因的转录激活因子，包括细胞色素P450 2C13和α-1抗胰蛋白酶。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

订购信息

产品名称产品编号规格价格数量收藏夹

HNF-3β 抗体 (H-4)

sc-374376
200 µg/ml
RMB2377.00

HNF-3β (H-4): m-IgGκ BP-HRP 套装

sc-522716
200 µg Ab, 40 µg BP
RMB2662.00

HNF-3β 抗体 (H-4) X

sc-374376 X
200 µg/0.1 ml
RMB2377.00

HNF-3β 抗体 (H-4) AC

sc-374376 AC
500 µg/ml, 25% agarose
RMB3129.00

HNF-3β 抗体 (H-4) HRP

sc-374376 HRP
200 µg/ml
RMB2377.00

HNF-3β 抗体 (H-4) FITC

sc-374376 FITC
200 µg/ml
RMB2482.00

HNF-3β 抗体 (H-4) PE

sc-374376 PE
200 µg/ml
RMB2580.00

HNF-3β 抗体 (H-4) Alexa Fluor® 488

sc-374376 AF488
200 µg/ml
RMB2685.00

HNF-3β 抗体 (H-4) Alexa Fluor® 546

sc-374376 AF546
200 µg/ml
RMB2685.00

HNF-3β 抗体 (H-4) Alexa Fluor® 594

sc-374376 AF594
200 µg/ml
RMB2685.00

HNF-3β 抗体 (H-4) Alexa Fluor® 647

sc-374376 AF647
200 µg/ml
RMB2685.00

HNF-3β 抗体 (H-4) Alexa Fluor® 680

sc-374376 AF680
200 µg/ml
RMB2685.00

HNF-3β 抗体 (H-4) Alexa Fluor® 790

sc-374376 AF790
200 µg/ml
RMB2685.00

HNF-3β (H-4) 中和勝肽

sc-374376 P
100 µg/0.5 ml
RMB511.00

HNF-3β抗体(H-4): sc-374376の無料試供品をいただきたいのですが，可能でしょうか？ワークすれば購入の予定です．

Asked by: Anonymous
Thank you for your question. Please contact our Technical Service Department for more information on how to receive a free 10 ug sample of this monoclonal antibody. Our Asian Technical Service team is available at (+86) 021-60936350 or [email protected] or by live chat on our website.
Answered by: Technical Support 15
Date published: 2019-06-05

Hi, I plan on using this antibody for immunohistochemistry on floating brain tissue, would it be possible to get a sample of the antibody to test before we buy it? Thanks.

Asked by: Bondarenko
Thank you for your question. Please contact Technical Service for information on how to obtain a sample.
Answered by: Technical Support
Date published: 2018-12-14

What application is the blocking peptide sc-374376 P appropriate for?

Asked by: Germaine
Thank you for your question. The blocking peptide is intended for use as a negative control, by pre-adsorbing the mouse monoclonal antibody against the antigen. For full protocol details, please contact our Technical Services Department or view our online protocol here: https://www.scbt.com/scbt/resources/protocols/peptide-neutralization
Answered by: Technical Support
Date published: 2017-02-24
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Rated 5 out of 5 by from Very good antibodyWorks as we expected. Tested by immunofluorescence. Great
Date published: 2018-12-18
Rated 5 out of 5 by from Nuclear localization in IFThe sample of the antibody worked quite well for IF in a dilution of 1:200
Date published: 2018-01-24
Rated 3 out of 5 by from Works but not specificWe bought this antibody to detect FOXA2 in prostate cancer cell lines. Based on published literature, FOXA2 protein is expressed in PC-3 cells but not in LNCaP cells. As the figure showed, the antibody works but can pick up non-specific bands in LNCaP cells.
Date published: 2017-12-15
Rated 4 out of 5 by from ICC on iPSC DA neuronal cultureI tested the antibody for an ICC on iPSC DA neuronal culture, fixed 20min in paraformaldehyde 4%. Dilution: 1/100. It worked but there is a little bit of noise.
Date published: 2017-10-31
Rated 4 out of 5 by from Immunohistochemistry free floatingI tested the antibody for an immunohistochemistry on mouse embryo spinal cord at E12.5, fixed 1h in paraformaldehyde 4%. Dilution: 1/100, Permeabilization solution: PBS 1X- Triton 0,2% - Goat serum 10%. It worked but there is a little bit of noise.
Date published: 2017-10-27
Rated 4 out of 5 by from gute Western BlotergebnisseWB mit Zelllysaten, Verdünnung 1:200, gute Signale mit ein wenig Backgroundsignal
Date published: 2017-10-18
Rated 5 out of 5 by from HNF-3beta/FOXA2 in neonatal rat brainClear immunoreactivity in neonatal (PN2) rat brain, and absence of immunoreactivity in adult rat brain. Note only specific brain regions were examined. Used at 1:50, signal may be stronger with different dilutions/conditions.
Date published: 2017-08-22
Rated 5 out of 5 by from great featuresWB on mice Liver sample，diluted with 1：200,which shows good quality and got bands very clearly.
Date published: 2017-04-30
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HNF-3β 抗体 (H-4) is rated 4.5 out of 5 by 10.
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